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Citations for Qiagen :
1751 - 1800 of 3706 citations for Monocyclohexyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted from stationary broth culture using genomic DNA-Tip 100/G system and buffer set (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Microbiology 2023Quote: A total of 100 ng total RNA was used for library preparation via the QIAseq miRNA Library Kit (#331502, Qiagen) according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cDNA was subsequently isolated by separation in a 3% agarose gel and isolation of cDNAs 200 to 600 base pairs in length (corresponding to 100 to 500 nucleotide RNAs plus adaptors) using QIAquick gel extraction kit (Qiagen) according to manufacturer’s recommendation ...
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bioRxiv - Genomics 2023Quote: ... 50 μl each input and purified RNA was adjusted to 100 μl with nuclease-free water and purified on RNeasy columns (Qiagen) using the modified protocol as described (Duffy and Simon ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 350□μl of 100% ethanol was added to the sample and loaded onto a RNeasy MinElute column (QIAGEN). RNA was isolated using RNeasy Mini kit (#74104 ...
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bioRxiv - Cancer Biology 2022Quote: ... Short-fragment size-selection was performed by cutting out gel bands between 100-200 bp and subsequent purification using the QiaQuick gel extraction kit (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was spun out of the Feather cartridge at 4000x g and added 100 µL of Ni-NTA agarose resin (Qiagen) to capture HIS-tagged proteins ...
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bioRxiv - Plant Biology 2023Quote: ... The DNA was extracted from approximately 100 mg of the plant tissues using a DNA isolation kit (PowerSoil, Qiagen, USA) following the manufacturer’s instructions ...
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Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomatobioRxiv - Plant Biology 2023Quote: ... DNA was extracted from 100 mg of ground frozen leaf and fruit tissue using Qiagen DNeasy Plant Mini Kit (Qiagen) following the manufacturer’s instructions and resuspended in 30 μL H2O ...
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bioRxiv - Microbiology 2023Quote: ... equal amounts (200 µl) of FruK and Cra cell lysates were mixed with 100 µl of nickel-nitrilotriacetic acid beads (Qiagen). After overnight incubation at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 100 to 150 mg of powder was used to extract total RNA using the RNeasy Plant Mini Kit from QIAGEN. On-column DNase treatment was performed using the RNase-free DNase kit from Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... the upper aqueous phase containing RNA was mixed with equal amounts of 100% ethanol and was then loaded onto an RNA spin column (Qiagen) and centrifuged for 30 seconds at 8000 rpm ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from the bacterial strains grown in VL55 medium with 5 mM pyruvate using the Qiagen Genomic-tip 100/G DNA isolation kit and associated DNA buffers (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription of 100 ng total RNA from each sample to cDNA was accomplished with the QuantiTect Reverse Transcription Kit (Qiagen) at 42°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
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bioRxiv - Microbiology 2024Quote: ... Pure plant gDNA was extracted from 100 mg of healthy potato (Solanum tuberosum) tissue using the Qiagen DNeasy Plant Mini Kit (Qiagen). Crude lysate from plant material was extracted using the Plant Material Lysis Kit (Optigene ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was isolated from 10-100 mg lyophilized leaf material using the DNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). DNA amount and quality were measured with the QubitTM 4 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: Total RNA was extracted from endothelial cells after stimulation with ImP (100 nM) or control for 12 h using the RNeasy Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Plants were ground in liquid N2 using a pestle and mortar and mRNA was extracted from 100 mg of leaf powder using the RNeasy Plant Mini kit (Qiagen) according to manufacture instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Seedlings were ground in liquid N2 using a pestle and mortar and mRNA was extracted from 100 mg of leaf powder using the RNeasy Plant Mini kit (Qiagen) according to manufacture instructions ...
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2019Quote: ... The filtered supernatant was loaded onto a Ni2+ -chromatography column (5 ml, Qiagen) and washed in 20 CV buffer (50 mM Tris ...
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bioRxiv - Cancer Biology 2020Quote: ... and digested with protease K in a concentration of 20 mg/ml (Qiagen). After several washing steps ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was then loaded onto 3 ml of Ni-NTA resin (Qiagen) by gravity at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Nhp6 was pulled down with 0.5 ml packed Ni-NTA agarose beads (Qiagen) for 3 hours at 4°C with gentle agitation ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was incubated with 2.0 ml of Ni-NTA resin (Qiagen, 30210) for 1 h at 4°C with rotation and the resin was applied to a column ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed with 3 column volumes of lysis buffer and proteins were eluted stepwise with lysis buffer supplemented with 50 and 250 mM of imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleavage products were passed through a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with 5 column volumes of lysis buffer supplemented with 50 mM imidazole to remove the cleaved tag and the TEV protease ...
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bioRxiv - Immunology 2020Quote: ... coli in LB Broth with 100ug/mL ampicillin using Qiaprep Spin columns (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... This periplasmic extract was then incubated with 5 ml Ni-NTA beads (Qiagen) at 4°C for 15 minutes and the resin slurry was packed on an open chromatography column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Immunology 2021Quote: ... with 10 μL/mL β-mercaptoethanol and mechanically disrupted with a TissueRuptor (Qiagen), before RNA extraction ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to three 5 ml Ni-NTA Superflow cartridges (Qiagen). The resin was washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) in order to remove the uncleaved fusion protein and His6-TEV protease ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Genomics 2021Quote: ... 1XPIC) with or without 0.1l of 100mg/ml RNaseA (Qiagen cat. no. 19101) for 15 min on ice briefly tapped few times during incubation time and then centrifuged at 1000g for 5 min at 40C ...
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bioRxiv - Biochemistry 2020Quote: ... 14 and 22 mg/mL for initial screening using commercially available screens (Qiagen) by sitting-drop vapor diffusion using a Crystal Gryphon robot (Art Robbins Instruments) ...
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bioRxiv - Molecular Biology 2021Quote: ... Filters were stored in 1.5 mL tubes with tissue lysis buffer ATL (QIAGEN) and proteinase K (QIAGEN) ...
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bioRxiv - Genomics 2021Quote: ... >300mAU (500μL of >600 mAU/ml solution) Proteinase K (Qiagen Cat No. 19131) was added to the sample and incubated at 55 °C for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from four ml of overnight culture (DNeasy kit, QIAGEN).
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was loaded on a 10 ml Ni-NTA Superflow column (QIAGEN), washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from 5 ml samples using the miRNAeasy mini kit (Qiagen), treated with TURBO DNase (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... The media was then incubated with 1.5 mL of Ni-NTA beads (Qiagen) at 4°C on a rolling bed for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... Two ml of a 50% slurry of washed Ni-NTA beads (Qiagen # 30210) was added to the supernatant of the 2-liter culture ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialysate was loaded onto 5 ml Ni2+-NTA column (Qiagen, Valencia, CA). After washing the column with buffer A containing 20 mM imidazole ...