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Citations for Qiagen :
1751 - 1800 of 2361 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... or Disp-/- cells were seeded into six-well plates and transfected with 1 µg Shh constructs together with 0.5 µg Scube2 or empty cDNA3.1 by using Polyfect (Qiagen). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of the isolated total RNA was transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). The quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of total DNase-treated RNA using the QuantiTect Reverse Transcription Kit (Qiagen) in 20 µL final volume according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Primers targeting the Mor-1 promotor were designed using the PyroMark Assay Design 2.0 software (Qiagen, Hilden, Germany) and were based on the Ensembl mRatBN7.2 genome build (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: ... The amplified product was diluted with a ratio of 1 part cDNA to 20 parts EB (Qiagen, 1014609), and concentrations were measured with a dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit ...
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bioRxiv - Immunology 2024Quote: Total RNA was purified from 0.5-1 million γδ T cells using the RNeasy Plus mini kit (Qiagen) according to the manufacturer’s instructions and the TCR library was prepared using iRepertoire arm-PCR platform-RNA-iR-Complete Dual Index Primer Kit - (iRepertoire) ...
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bioRxiv - Plant Biology 2024Quote: ... TAW1 and its homologues with their annotation IDs are listed in Supplementary Table 1. Amino acid sequences were aligned using CLC Workbench (v. 24.0.1) (Qiagen). Unrooted phylogenetic trees were generated by the neighbour-joining method with 1000 bootstrap replicates in CLC Workbench.
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from approximately 1 million cells using the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) and following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1×106 mNPCs were collected on two separate days and resuspended in 600 µl RLT buffer (#79216, Qiagen). RNA was isolated using RNeasy column purification (#74104 ...
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bioRxiv - Genomics 2024Quote: ... the isolated footprinted DNA (1 µg per sample) was bisulfite converted with the Epitect bisulfite conversion kit (QIAGEN), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... We plotted the change in fluorescence versus temperature (dF/T) using Rotor-Gene Q software (version 2.3.5, build 1; QIAGEN) and compared HRM peaks of amplified samples to bulk genomic DNA and plasmid controls.
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 0.08 µg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 µg of pAAV2-G12-AkaLuc (0.4 µg per well) using Effectene transfection reagent (Qiagen). Culture media was changed 8 h after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of primer mix (with a fluorescently labelled forward primer) at 0.2 μM and 1 μL QIAGEN Multiplex PCR Master Mix (QIAGEN). PCR cycling conditions were 95 °C for 10 min followed by 35 cycles of 95 °C for 30 s ...
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bioRxiv - Pathology 2022Quote: ... RNA was isolated from maternal and fetal tissues (Table 1) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The tissue was homogenized for 1 min at 28.5 Hz in a TissueLyser (Qiagen TissueLyser II, Retsch GmbH, Germany) before samples were rotated end over end for 1 hour at 4°C and centrifuged at 18320g for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were then resuspended in 500 μL of supernatant and stabilized with 1 mL of RNAprotect Bacteria Reagent (Qiagen). The RNeasy® Protect Bacteria Mini Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...
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bioRxiv - Molecular Biology 2021Quote: HIV-1 viral RNA was extracted from 20-50 ul of plasma using QIAamp Viral RNA mini kit (Qiagen). qRT-PCR was performed using a TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genomics 2020Quote: ... the final clean-up was performed at 0.9:1 ratio of beads to PCR products and elution again done in 16μl EB (Qiagen, Germany). Samples were diluted to 8pM final concentration before sequencing on an Illumina Miseq (2×25nt).
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bioRxiv - Pathology 2020Quote: Total RNA was extracted from the BALF sample of patient 1 using the RNeasy Plus Universal Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HOS cells were cotransfected with a Rev/Env-encoding pSVIIIenv plasmid and a Tat-encoding plasmid at a 1:0.125 ratio using the Effectene transfection reagent (Qiagen). At 48 h after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed on cDNA dilutions of 1/20 in the presence of 7.5-pmol primers with QuantiTect SYBR Green PCR Master Mix (Qiagen) on a Lightcycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed according to the manufacturer’s recommendations on 1 μg of RNA with the QuantiTect Reverse Transcription Kit (Qiagen), which includes a genomic DNA–removal step ...
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bioRxiv - Microbiology 2020Quote: ... A total of OD600 = 0.3 in 1 ml of cells was pelleted and gDNA was extracted using DNeasy Blood & Tissue kit (Qiagen) using the Gram-negative bacterial protocol followed by quantification using Qubit ...
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bioRxiv - Plant Biology 2021Quote: ... ABI1 and ABI2 with primers listed in Supplemental Table 1 using the Rotor Gene SYBR Green PCR Kit (Qiagen) according to manufacturers’ protocol in a Rotor gene Q cycler (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... Fastq files were mapped to KSHV genome (GenBank accession no. AP017458 for SPEL, and NC_003409 for BCBL-1) by CLC Genome Workbench (version 14, Qiagen), and the coverage data were obtained using Integrative Genomics Viewer (version 2.50 ...
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bioRxiv - Microbiology 2020Quote: Each dissected compartment (legs and thoraxes) was homogenized individually 3 x 1 minute at 30 Hertz using TissueLyser (Qiagen) and glass beads (#11079110 ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... after which a second volume of 400μL a 1% β-mercaptoethanol in RLT buffer (AllPrep DNA/RNA Minikit, Qiagen) was added after steel bead removal ...
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bioRxiv - Physiology 2021Quote: ... 10-50 mg tissue was homogenized (1 mM EDTA and 4 mM sodiummetabisulfite, pH 7.4) using a TissueLyser II (Qiagen). Samples where adjusted to the same weight/volume percentage by adding a volume of homogenization buffer and the NA content was measured by ELISA (BA E-5200 ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mg of freeze-dried and ground roots were mixed with 1.6 mL of 1 M perchloric acid and homogenized in a TissueLyser II (Qiagen) in microtubes containing 6 glass beads of 2.8 mm in diameter ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Genetics 2022Quote: Total RNA was isolated from PWS and control INS-1 lines grown as above (7.5 mM glucose) by Trizol harvest and miRNeasy (Qiagen) column purification ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was washed three times with ice-cold PBS and suspended in 1 ml TRIzol™ reagent (QIAGEN). Instead ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×104 cells of each sample were lysed in 100 µl of RLT Plus Lysis Buffer (Qiagen, Hilden, Germany) supplemented with 1% 2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... and stored pellets at −80 °C for <1 week before RNA extraction using RNeasy Protect Bacteria Mini Kit (Qiagen) according to the manufacturer’s instructions (protocol #5) ...
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bioRxiv - Cancer Biology 2022Quote: 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and WES was conducted and sequenced by BGI ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of bulk genomic DNA was extracted from gastric tumors using the DNeasy Blood and Tissue Kit (Qiagen) and sonicated using the Covaris instrument ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... miR-67 (miR control) or empty vector at a ratio of 1:3 using a lipofection protocol (Effecten, Qiagen) and processed at DIV15 ...
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bioRxiv - Microbiology 2020Quote: Airway epithelia cultured on 6.5-mm Transwell membranes were washed and treated for 1 minute at RT with RLT buffer (Qiagen) with 1% β-mercaptoethanol (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... cancer-associated fibroblasts (CAFs) and C666-1 cells using RNeasy Mini kit per the manufacturer’s instructions (Qiagen, Hilden, Germany). RNA integrity number (RIN ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were confirmed by agarose gel electrophoresis using 0.8-1% agarose and purified using PCR purification kit (Qiagen). In case of NS1 gene ...
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bioRxiv - Microbiology 2021Quote: ... with sequences and concentrations listed in Table 1 in combination with QuantiTect Multiplex PCR Kit (Qiagen, Germantown, MD, USA). PMAxx (Biotium ...
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bioRxiv - Microbiology 2022Quote: Frozen tissue was partially thawed and submerged in lysis buffer containing 1% β-mercaptoethanol and 0.5% Reagent DX (Qiagen) before tissues were homogenised together with TissueRupture (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...