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Citations for Qiagen :
1751 - 1800 of 3689 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Each 1-kb fragment was purified using a gel extraction kit (Qiagen) and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM imidazole (final concentration) and 1 ml Ni-NTA resin (Qiagen) pre-washed in 25mM Hepes ...
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bioRxiv - Microbiology 2021Quote: ... multivorans 1 genomic DNA (gDNA, extracted with DNeasy Blood & Tissue Kit, Qiagen), cleaned up using the Monarch PCR Purification kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... amplicons were subjected to the QIAseq 1-Step Amplicon Library Kit (Qiagen) for Illumina compatible next generation sequencing (NGS ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: ... containing 10μl of 1% v/v β-mercaptoethanol TCL buffer (Qiagen # 1031576) using a BD AriaII cell sorter ...
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bioRxiv - Immunology 2020Quote: ... Tissue was homogenized at 30hz for 1 minute in a TissueLyser (Qiagen) then briefly spun at maximum RPM in a benchtop centrifuge to pellet large debris ...
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bioRxiv - Genomics 2020Quote: ... and purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from 1 million cells by RNeasy columns (QIAGEN). We used 2 µg of RNA to perform reverse transcription with random primers using TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix TransGen Biotech ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was mixed with 1 ml Ni-NTA-Agarose slurry (Qiagen) in an overhead rotator (10 rpm ...
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bioRxiv - Microbiology 2023Quote: ... Dilutions of 104-101 plasmid copies contained 1 ng carrier RNA (QIAGEN). QPCR was performed in a LightCycler 480 Instrument II (Roche ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 1 × 106 cells using the RNeasy kit (Qiagen), and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: THP-1 and primary macrophage RNA was extracted using RNeasy kit (Qiagen), followed by cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended with 1 mL of QIAzol (QIAGEN Inc.; Germantown, MD) and transferred to tissue homogenizing CKMix tubes 2mL (Bertin Instruments ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using QuantiNova SYBR Green 1 Step kit (Qiagen) with 40 cycles of amplification at 57ᵒC performed on an ABI 7500 (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour and then transfected with siRNAs (Qiagen, Cat. No. 1027417) using lipofectamine RNAiMAX (Thermo-Fisher Scientific #13778 ...
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bioRxiv - Physiology 2023Quote: ... Agarose beads were collected in 1 ml propylene gravity flow columns (Qiagen) and washed with 10 ml Resuspension buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 200 µg mL-1 RNaseA (Qiagen, 19101), 50 µg mL-1 propidium iodide (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were homogenised in 1 ml QIAzol lysis reagent (Qiagen, Hilden, Germany) with a handheld tissue homogeniser (Appleton Woods Ltd ...
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bioRxiv - Cancer Biology 2023Quote: ... with the addition of 1% B- mercaptoethanol using the TissueLyserII (85300, Qiagen) in combination with 5mm stainless steel beads (69989 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Cells were harvested in 1 mL QIAzol Lysis Reagent (Qiagen, Hilden, Germany) for 5 min at RT and 200 µL chloroform (Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... These tissue pools were directly immersed in 1 ml QIAzol (Qiagen, Germany) and stored at -80°C until further processing ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1 mL of Ni-NTA agarose (Qiagen) pre-equilibrated with lysis buffer via nutation at 4 °C for 1 hr ...
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bioRxiv - Neuroscience 2024Quote: ... The frozen samples were pulverized for 1 min using a TissueLyser (Qiagen) set to 25 Hz for 1 min.
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bioRxiv - Microbiology 2024Quote: ... 1 mL of Ni-NTA agarose resin (Qiagen, 0.5 mL bed volume) was equilibrated in lysis buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... The resulting 1.5 L bacterial culture was incubated at 37°C for 2h and a Maxiprep performed using Qiagen MaxiPlus kit (Qiagen #12963) to a resulting 1 mg of plasmid DNA ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were transfected with 50 nM double-stranded siRNA oligonucleotides (Supplementary Table 3) using HiPerFect Transfection Reagent (Qiagen, Crawley, UK).
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bioRxiv - Immunology 2021Quote: ARNO siRNA (Mm_Pscd2_3) or a negative non-specific siRNA control (Allstar siRNA) were transfected into SFs using HiPerFect transfection reagent (all Qiagen,UK) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted from 3-week-old leaves of ALO seedling using the QIAGEN® Genomic DNA Extraction Kit (Cat. 13323, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... 3-5 million cells were then harvested and genomic DNA was isolated using QIAamp DNA mini kit (QIAGEN, prod. number 51304). The following L1 recovery steps were adapted from24,52 with modifications ...
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bioRxiv - Neuroscience 2019Quote: ... Synthesis of cDNA was performed on 0.3-1.0 μg of normalized total RNA from each sample using QuantiTect Reverse Transcription kit (Cat No. 205313, Qiagen, Valencia, CA) which included a DNase treatment step to remove any residual genomic DNA contamination ...
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bioRxiv - Genomics 2021Quote: ... DNA corresponding to the size of mononucleosomes (100-200 bp) was re-isolated from a 3% agarose gel using MinElute Gel Extraction Kit (Qiagen, # 28604). As input ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Approximately half the volume of each feces sample (100-200 mg) was immersed in 3 ml of Inhibit EX Buffer (QIAGEN, Hilden) and stored at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The following primers were used to assess the relative level of GAT-3 mRNA: Slc6a11 Rn.10545 in comparison to that of cyclophilin A (Peptidylprolyl isomerase A) used as housekeeping gene (Qiagen, UK). Real-time-PCR was performed on the CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from 3 biological replicates of each U2OS time point according to manufacture instruction using the RNeasy Plus Mini Kit (QIAGEN, #74134). Isolated RNA was reversely transcribed by using first-strand cDNA synthesis kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR was performed in an Applied Biosystems QuantStudio 3 thermocycler using the QuantiNova Probe SYBR Green PCR Kit (Qiagen, Germany); mix proportions and cycling parameters were used as described in manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The lysate was then clarified by centrifugation at 16,000 x g for 45 minutes and then applied to 3 mL Ni-NTA resin (Qiagen, Valencia, CA) equilibrated with buffer A ...
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bioRxiv - Microbiology 2022Quote: ... and the amplified DNA bands from the 5’ and 3’ ends were individually excised and purified with QIAquick® Gel Extraction Kit (QIAGEN). Purified PCR products were cloned into pJET1.2/blunt plasmid (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: 1-3 million of feeder-independent mESCs were harvested for genomic DNA extraction by using a QIAamp DNA Mini Kit (QIAGEN, 51306). Genomic DNA concentration was determined with a Nanodrop spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... and organoids at the end of passage one subjected to two weeks of differentiation (n=3) using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and RNA was purified after 3 days from the second transfection using the RNeasy Plus Mini Kit (Qiagen, Catalog number 74134) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... Cells from 3 independent biological replicates of MG1655 in each modified medium were treated with RNA protect bacteria reagent (Qiagen, Germany), to prevent degradation of RNA ...
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bioRxiv - Genomics 2022Quote: ... Cells were maintained at 37°C in 5% CO2 for 3 days before collecting genomic DNA using DNeasy Blood & Tissue Kits (Qiagen, 69504) and sequencing.
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from the stool of enrolled mice (3 mice per group) using the QIAamp DNA Stool Mini kit (Qiagen, Germany). The extracted DNA was amplified using primers targeting the V1 to V3 hypervariable regions of bacterial 16S rRNA gene (27F ...
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bioRxiv - Immunology 2019Quote: Total RNA yields from the PBMC and stimulated PBMCs were in the range of 3 micrograms (50-100 ng/μl in 30μl Qiagen EB buffer) determined by absorbance at 260nm on the NanoDropTM One (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from aerial parts of 3-week-old seedlings grown on soil using either RNeasy Plant Mini Kit (Qiagen, 74904) or Monarch Total RNA Miniprep Kit (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... RT-PCR products were gel purified in 3% agarose gel and cleaned up using QIAGen gel extraction kit (QiaGen, Hilden, Germany).
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bioRxiv - Cell Biology 2021Quote: ... The plasmid DNA was extracted from 3-9 positive colonies where possible after blue-white screening using a QIAprep® Spin Miniprep Kit (Qiagen) and sequenced using the standard T7 primer at Eurofins Genomics ...
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bioRxiv - Cancer Biology 2022Quote: ... and GSC6-27 HEPACAM shRNA (n=3) cultures were washed in cold 1X PBS and total RNA was extracted using RNeasy Plus Mini Kit (Qiagen, 74134) following manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... Trimming of the obtained sequencing reads and mapping to the pea aphid genome (version 3) were performed with the program CLC Genomics Workbench (Qiagen Bioinformatics) (mapping parameter ...