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Citations for Qiagen :
1701 - 1750 of 10000+ citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: DNA was purified for sequencing from cells infected in six well plates using a blood and cell culture DNA mini kit (Qiagen). Samples were sequenced at the Northwestern University Genomics Core Facility using NextGen Illumina HiSeq SR500 sequencing ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was extracted from puromycin selected SUDHL4 cells and untreated WT cells using the QIAamp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the cell culture supernatants were collected and cells were lysed using RLT Buffer from the RNeasy Mini Kit (#74106; Qiagen).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondria isolation from HEK293T cells was performed after 24 hours of cell transfection using the Q proteome Mitochondria Isolation Kit (QIAGEN). Cell lysis and mitochondria homogenisation were conducted as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... phenol-free total RNA was extracted from cells directly in cell culture wells using RNeasy Plus Micro Kit with gDNA eliminator columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cell lines grown in 6-well plates or from cell pellets using the RNAeasy Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted from each cell group cultured on cell culture dishes using the RNeasy Plus Mini Kit (74134; Qiagen) and converted to cDNA using the Verso cDNA Synthesis Kit (AB1453A ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Immunology 2021Quote: ... Total RNA from the cells and EVs were first isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 g x 10 min) and from infected cells using QIAamp Viral RNA Mini Kit and RNeasy Plus mini kit (Qiagen, Hilden, Germany), respectively ...
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bioRxiv - Genetics 2021Quote: ... and that of cultured cells was obtained using the AllPrep DNA/RNA/miRNA Universal kit or the miRNeasy mini kit (QIAGEN, Hilden, Germany). The samples were reverse-transcribed into cDNA with the High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Total RNA from the cells and EVs was isolated and purified using an RNeasy Mini Kit and a miRNeasy Serum/Plasma kit (Qiagen, Hilden, Germany), respectively ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... was extracted from whole cell RNA extracts (total RNA) using the miRNeasy Mini Kit and the RNeasy MiniElute Cleanup Kit (both Qiagen, Hilden, Germany). Extraction was performed according to the manufactureŕs instructions.
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bioRxiv - Microbiology 2023Quote: ... Clarified cell lysate was processed using a Qiagen RNeasy kit and in-column treatment with a DNase I kit (Qiagen, Germantown, MD) for purification of total RNA ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from Min6 cells using RNeasy Plus mini kit and from pancreatic islets using RNeasy Plus micro kit (Qiagen, ref 74034) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in a total volume of 10 μl having 5 μl Rotor-Gene SYBR® Green PCR Kit (Qiagen, NSW, Australia), 0.3 μl each of 10 μM forward and reverse primer and 2 μl of genomic DNA ...
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bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
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bioRxiv - Microbiology 2021Quote: ... Intact bacterial cells were pelleted by centrifugation at 10,000 x g for 5 min at room temp and DNA extracted from the pellet using a DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). DNA concentration was assessed by the QubitTM dsDNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Following the manufacturer’s instructions RNA was reverse-transcribed in a 20 μl reaction volume (42°C, 30 min; 95°C, 5 min) using a QuantiTect Reverse Transcription Kit (Qiagen, Valencia, CA, USA). cDNA was then amplified using a SYBR Green I Master mix (Roche ...
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bioRxiv - Genomics 2021Quote: ... we pooled 1.5 mg RNA from each sample (mixed-stage, male-enriched, and starved) and used the RNeasy MinElute Cleanup kit (Qiagen, catalog no. 74204) to further purify and concentrate the pooled RNA ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 minutes at 300xg and pellets resuspended in Buffer RLT Plus (RNeasy Plus Mini Kit from Qiagen, Germantown, MD, USA), and frozen at −80°C ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Bioengineering 2023Quote: ... The RT-qPCR experiments used Student’s t-test of the experimental group compared to the sham control (Qiagen GeneGlobe RT2 Profiler PCR Data Analysis) ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA from 60 million unsorted cells (500× coverage of the GeCKO v2 library) and 6 million sorted cells was isolated using Blood & Cell Culture DNA Midi Kit (QIAGEN, 13343) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... total RNA was extracted from cells from stable-cell lines created using a RNeasy Plus Mini Kit (Qiagen, catalog number: 74134) following the Manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were then rinsed with 1X PBS and processed for subcellular fractionation (Qproteome Cell Compartment Kit, Qiagen; following manufacturer’s protocol). Nuclear fractions were isolated and protein precipitated by incubation in 4x volume of ice-cold acetone for 15 minutes followed by pelleting (10 min ...
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bioRxiv - Microbiology 2020Quote: Total cellular DNA was extracted from cells (cell lines or PBMCs) using a QIAmp DNA Blood mini kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from untreated cells (24 h) and IFNγ-treated cells (6 or 24 h) were extracted with RNeasy plus mini kit (Qiagen, 74134) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted for each of the persistently infected cells as well as the cell lines without NDV infection (negative control) using Qiagen RNeasy micro kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysis and RNA extraction from siRNA transfected cells were performed using the RNeasy RNA extraction kit (Qiagen Inc, Germantown, MD). 1 µg of total RNA was reverse transcribed with the High-capacity RNA-to-cDNA kit (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: E3 and E4 primary microglia were plated at 5×106 cells/well in 6-well plates and RNA was extracted from the cells using the RNEasy Plus Mini Kit (Qiagen #74136) and converted to cDNA using High-Capacity RNA-to-cDNA kit (Thermo #4387406 ...
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bioRxiv - Genomics 2019Quote: ... and the remaining cells in single-cell capture (T1, T2, and T3 samples) using the QIAamp DNA Mini kit (Qiagen, 51304), and 500 ng of DNA were used to construct exome sequencing libraries with the same reagents as the single cell DNA-seq.
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bioRxiv - Genomics 2021Quote: ... the cells were collected and total RNA was extracted from the cells using the Qiagen RNeasy® Plus Kit (Qiagen, 74134). One µg of RNA was then used to synthesize cDNA using the Bio-RAD iScript cDNA Synthesis Kit (Bio-RAD ...
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bioRxiv - Cell Biology 2019Quote: ... MCMBP depleted cells using siRNA and MCMBP-KO (clone #1 and #2) cells was isolated using RNeasy Mini Kit (Qiagen, 74104). The cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A pellet of 200-400 µl of animals was resuspended in 400 µl Cell Lysis Solution (Qiagen Gentra Puregen Cell kit) with 5 µl proteinase K (20 mg/ml ...
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bioRxiv - Evolutionary Biology 2022Quote: ... log-phase cells in the range of 1–2 × 108 cells using the Qiagen DNeasy® Blood and Tissue kit (Qiagen). We resuspended the genomic DNA in 10 mM Tris-HCl pH 8.5 and stored it at 4° until submission to the McDonnell Genome Institute at Washington University in St ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from CD33+ ITGA7-sorted primary AML cells (> 4x105 cells/sample) using the RNeasy Mini kit (Qiagen, 74106) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Molecular Biology 2024Quote: HBEC cells (5e5) from different treatments were mixed mouse RPE1 cells (5e5) for harvesting total RNA using RNeasy kits (Qiagen #74104) for reverse transcription with iScriptTM reverse transcription supermix (Bio-Rad #1708840 ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼5×107 live cells were harvested per replicate and cell pellets underwent same-day nuclei preparation using Qiagen Blood & Cell Culture DNA Maxi Kit (Qiagen, #13362) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cells were seeded in 6-well and 12-well plates at a density of 1.25×105-5×105 cells per well and transfected with miR-320a mimic (Qiagen, 339173 YM00471432) or antagomir (GenePharma ...
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bioRxiv - Immunology 2021Quote: ... Single cells were sorted into individual wells of a 96-well plate containing 5 μl of lysis buffer (TCL buffer (Qiagen #1031576) with 1% of 2-b-mercaptoethanol) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...