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Citations for Qiagen :
1651 - 1700 of 2414 citations for 8 Chloro 2 methylimidazol 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Dual RNA-seq identifies proteins and pathways modulated during Clostridioides difficile colonisationbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 1 mL buffer RLT from the RNeasy mini kit (Qiagen, Germany) with a 1:200 dilution of β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The lysates were then incubated for 1 h with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) at the indicated temperatures (4°C or room temperature) ...
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bioRxiv - Microbiology 2023Quote: Total DNA was extracted from frozen cell pellets of 0.3-1×106 PBMCs (Qiagen, Germantown, MD), and concentrated using an ethanol precipitation protocol with GlycoBlue following manufacturer’s instructions (Thermofisher ...
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bioRxiv - Biochemistry 2023Quote: ... The cell lysate was clarified by centrifugation and incubated with 1 mL Ni-NTA resin (Qiagen). The precursor protein was purified using the standard protocol from Qiagen for purifying protein from Ni-NTA resin under denaturing conditions with urea ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... with each 20 μL reaction consisting of 1× QuantiFAST reaction mix containing ROX reference dye (Qiagen), 0.66 µM forward and reverse primers ...
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bioRxiv - Plant Biology 2023Quote: ... 1 µg of RNA was reverse transcribed into cDNA using the QuantiTect Reverse Transcription Kit (Qiagen). Transcript quantification was performed in 96-well plates ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour at room temperature followed by isolation with a minElute RNA Cleanup Kit (Qiagen). RNA concentrations were measured by Nanodrop and total RNA integrity assayed using an Agilent Bioanalyzer ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg of total mRNA from each sample was retrotranscribed using QuantiTech reverse Transcription Kit (Qiagen). Real-Time PCR was performed using SYBR Green Master Mix (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were loaded onto a 1 mL Ni-NTA Superflow column (Qiagen, Valencia, CA, USA) that had been equilibrated with 10 column volumes of wash 1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant proteins were purified on Ni-NTA columns (1 ml Ni-NTA Agarose; Qiagen Cat # 30210), washed with 20 ml hexokinase buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... Bacteria (∼1 × 109 cells) were removed from the cultures and suspended in RNAprotect bacteria reagent (Qiagen), pelleted by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... genomic DNA was extracted from 1 ml of overnight culture (DNeasy Blood and Tissue Kit—Qiagen) pre-treated with 100 μg of lysostaphin (Sigma cat ...
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bioRxiv - Microbiology 2023Quote: ... These were harvested by centrifugation and resuspended in 1 mL of RNA Protect Bacteria Reagent (Qiagen) with 30µL of 20 mg/mL lysozyme ...
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bioRxiv - Genomics 2023Quote: ... cfDNA was isolated from 1 mL of plasma using the QIAGEN Circulating Nucleic Acids Kit (QIAGEN), eluted in AE buffer ...
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bioRxiv - Physiology 2023Quote: ... PCR was performed using 1 μl of cDNA and Top Taq DNA polymerase and buffer (Qiagen). The reactions were performed for 30 s at 94 °C ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: Bulk or flow-sorted THP-1 macrophages were lysed with β-ME-supplemented Buffer RLT (Qiagen). RNA was isolated from the lysate using an RNEasy plus kit (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells were transfected with 1 μg of pUbg-R252 plasmid using Effectene transfection reagent (QIAGEN) in a 60 mm dish following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1 mL of PE buffer and 100 µL of EB buffer (MinElute PCR Purification Kit, QIAGEN). The silica columns were placed in a collection tube to dry and later in a 1.5 mL DNA lo-bind tube (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of PBS-AB was added to each tissue with Tungsten carbide 3mm beads (Qiagen). Samples were homogenized for 15 min and then centrifuged at 15,000 g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA (gDNA) was extracted from 1-5×106 cells using DNeasy Blood and Tissue Kit (Qiagen) and 250 ng gDNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was recovered using a chloroform-ethanol procedure (Supplementary protocol 1) and diluted in AE buffer (Qiagen). A pair-ended library was constructed by Eureka Genomics (Hercules ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA was extracted from 1 ml of overnight culture using a QIAamp DNA Mini Kit (Qiagen). Illumina MiSeq sequencing operated by the Functional Genomics Centre Zurich and Novogene (Cambridge ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibodies were used at the following dilutions: 1:1000 anti-penta-His mouse monoclonal (Qiagen), 1:5000 anti-cMyc mouse monoclonal (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... and also performed direct mapping against reference genome Wuhan/Hu-1/2019 (NCBI Nucleotide – NC_045512, GenBank – MN908947) using the CLC Genomics Suite v5.1.0 (QIAGEN). We then used SAMtools v1.3 to sort the aligned bam files and generate alignment statistics (WysokerA ...
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bioRxiv - Immunology 2021Quote: DNA was isolated from 1 × 106 PBMC using the DNeasy Blood & Tissue Kit (Qiagen, Cat No 69506), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from CTi400 and N/TERT-1 cells using RNeasy Plus mini kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Beads were washed 4 times with 1 ml of IP buffer and 700 μl of Qiazol (Qiagen) was directly added to the beads ...
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bioRxiv - Microbiology 2019Quote: Total RNA from PGC fibroblast and DF-1 cells were extracted using an RNeasy mini kit (Qiagen), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were harvested and total RNA was extracted using the RNeasy Plus Mini kit (Qiagen). cDNA was synthesized from 0.1 μg total RNA using Superscript IV reverse transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... endobioticum pathotype 1(D1) isolate MB42 reference genome (31) in CLC genomics workbench v11.0.1 (Qiagen, the Netherlands), and mappings were improved with the local realignment tool (default settings ...
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bioRxiv - Genetics 2020Quote: ... cDNA synthesis was performed with 1 mg of total RNA using miScript II Reverse Transcription Kit (QIAGEN). See Supplemental Information for primers used in these experiments ...
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bioRxiv - Cell Biology 2021Quote: ... SF9 cells at 1×106 SF9 cells/ml were grown in 24 well round bottom plates (Qiagen) and infected with baculovirus of the indicated single subunits ...
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bioRxiv - Synthetic Biology 2021Quote: mESC gDNA was purified from 1-5 million cells using the QIAamp DNA mini kit (Qiagen 51306) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from 1 × 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Whole RNA (1-3 μg total per 10 μL volume) was isolated using RNAeasy mini kit (QIAGEN) or TRIzol (Life Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then vortexed briefly and 1 mL of ice-cold QIAzol Lysis Reagent (Qiagen, cat. 79306) was added to each sample ...
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bioRxiv - Microbiology 2020Quote: ... amplicons were purified from excised gel slices (1% agarose) using the Qiagen MinElute Gel Extraction kit (Qiagen). Each amplicon was quantified using Quant-IT HS reagents (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...
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bioRxiv - Microbiology 2021Quote: ... stored at −20° C and characterized by immnoblotting using mouse anti-2xStrep tag (Qiagen #34850, 1:1,000), and secondary goat anti-mouse IgG IRDye® 800CW (LI-COR # 926-32210 ...
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bioRxiv - Microbiology 2021Quote: ... Five hundred microliters of exponentially growing cells were stabilized in 1 mL of RNAprotect Bacteria Reagent (Qiagen). After treatment of the culture pellet with lysozyme (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) and cloned in pBluescript SK (+ ...
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bioRxiv - Neuroscience 2020Quote: The obtained ~2Kb amplicons were gel purified from 1% agarose gel using QIAquick Gel Extraction Kit (Qiagen) followed by clean-up with QIAquick PCR purification kit (Qiagen) ...
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“A Proteogenomic workflow reveals distinct molecular phenotypes related to breast cancer appearance”bioRxiv - Systems Biology 2020Quote: ... were added to each sample tube together with 400μL a 1% β-mercaptoethanol in RLT buffer (Qiagen) and 2μL of antifoam agent (ID 19088 ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... at a ratio of 1:0.6 with a final elution in 25 μl of EB Buffer (Qiagen). The cDNA was then quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2022Quote: RNA was extracted from skin biopsies (~1 cm2) taken from the upper backs of mice (Qiagen #74106). Each RNA sample was collected in 40 μL of ultrapure water (Thermo Fisher #10977) ...
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bioRxiv - Molecular Biology 2022Quote: ... including of multiple steps showed below: 1) Total DNA was extracted using QIAamp DNA Mini Kit (Qiagen); 2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1000:1 v/v) and DNA from individual colonies were isolated using QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... and passed through 1 mL of glutathione–Sepharose 4B and then onto a Ni-NTA column (QIAGEN) to capture the residual GST- and His-tag fragments ...