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Citations for Qiagen :
1601 - 1650 of 2499 citations for 6 Amino 5 2 2 diethoxyethyl 4 hydroxy Pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Expressed proteins were subsequently purified by Ni-chelate chromatography (Qiagen, 5 ml column) and SEC (S75 ...
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bioRxiv - Biochemistry 2019Quote: ... The filtered supernatant was loaded onto a Ni2+ -chromatography column (5 ml, Qiagen) and washed in 20 CV buffer (50 mM Tris ...
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bioRxiv - Immunology 2021Quote: RNA was isolated from ~5 x 105 cells using RNeasy mini kits (Qiagen), typically yielding 100-400 ng RNA ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were homogenized using 5 mm steel beads and a Tissuelyser II (Qiagen) operated at 30 Hz for 5 min ...
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bioRxiv - Pathology 2021Quote: PDGFRα+ and CD56+ cells (passages 3 to 5) were lysed in Qiazol (Qiagen) followed by chloroform/isopropanol total RNA extraction ...
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bioRxiv - Immunology 2020Quote: ... 5 U/ml dispase (Stemcell) and 50 mg/ml DNase I mix (Qiagen) in complete RPMI1640 medium ...
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bioRxiv - Molecular Biology 2021Quote: ... genomic DNA was isolated from 5 million cells using the DNAeasy kit (Qiagen). 4ug gDNA was digested with NlaIII or MseI ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10x CoralLoad PCR buffer and 5 µl of TopTaq Master Mix 2x (Qiagen). An initial denaturation cycle of 94°C for 6 min was followed by 35 cycles of 94°C for 45 s ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Physiology 2022Quote: ... mRNA samples were concentrated to ≤ 5 µl by MinElute column (QIAGEN, Cat. 74204). For generation of RNA-seq libraries ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed with 3 column volumes of lysis buffer and proteins were eluted stepwise with lysis buffer supplemented with 50 and 250 mM of imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The cleavage products were passed through a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed with 5 column volumes of lysis buffer supplemented with 50 mM imidazole to remove the cleaved tag and the TEV protease ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μl of 10% SDS and 5 ul of Proteinase K (Qiagen #19131) were added to each sample ...
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bioRxiv - Biophysics 2020Quote: ... This periplasmic extract was then incubated with 5 ml Ni-NTA beads (Qiagen) at 4°C for 15 minutes and the resin slurry was packed on an open chromatography column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 5mM EDTA (pH 8) together with a 5 mm stainless steel bead (Qiagen) and macerated at 1,200 rpm for 2 mins in the Geno/grinder followed by a 10-minute centrifugation (17,000 x g ...
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bioRxiv - Immunology 2019Quote: ... total RNA (5 mice per group) was extracted using RNeasy Micro Kit (QIAGEN). Samples were sent to Admera Health for sequencing and analysis ...
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bioRxiv - Zoology 2021Quote: ... 0.1 μL of 5 U/μL Taq DNA Polymerase (QIAGEN, Valencia, CA, USA), 0.2 μL of U19 fluorescent dye ...
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bioRxiv - Immunology 2020Quote: ... miRNA inhibitors (miRCURY LNA miRNA power inhibitor with or without 5’-FAM, Qiagen) were added directly to culture media at the time of activation and were taken up by the cells by spontaneous translocation across the cell membrane ...
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bioRxiv - Pathology 2019Quote: ... with sterile 5 mm stainless steel beads and processed using Tissuelyzer LT (Qiagen). The homogenate was lyzed with proteinase K and subjected to DNA extraction using the QIAmp DNA mini kit (Qiagen) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied to three 5 ml Ni-NTA Superflow cartridges (Qiagen). The resin was washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was applied to a 5 ml Ni-NTA Superflow cartridge (Qiagen) in order to remove the uncleaved fusion protein and His6-TEV protease ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... The supernatant was treated for 5 minutes at room temperature with RNAse (Qiagen) and then centrifuged again ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA was extracted from 5 organoids using the RNeasy Plus MiniKit (Qiagen). Purity was determined byA260–A280 ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS, PT0017_Covaris_20ng_XTHS, PT0017_Qiagen_20ng_Illumina ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted from 5 ml samples using the miRNAeasy mini kit (Qiagen), treated with TURBO DNase (Ambion ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg/mL DNase I (AppliChem) and 200 µg/mL RNase A (Qiagen) using 4 cycles of 15 s sonification at 30% duty cycle and 30% output control ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialysate was loaded onto 5 ml Ni2+-NTA column (Qiagen, Valencia, CA). After washing the column with buffer A containing 20 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... 37°C in 5% CO2 and purified with a RNeasy mini kit (Qiagen) according to the protocol for Gram-positive bacteria ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was mixed with 5 ml of Ni-NTA agarose (QIAGEN) pre-equilibrated with solubilization buffer for 60 min ...
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bioRxiv - Molecular Biology 2023Quote: mRNA was extracted from ∼5×106 cells using the miRNeasy Mini Kit (QIAGEN). Library preparation and sequencing was conducted by City of Hope Integrative Genomics Core ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples are next loaded onto a 5 mL Hitrap S column (Qiagen) pre-equilibrated with 50 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA from 5×107 cells was extracted using RNeasy mini kit (Qiagen). One μg total RNA was used for cDNA synthesis using Quantinova Reverse Transcription kit (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... homogenized using 5-mm steel beads and a tissue lyser (Qiagen TissueLyser II) 6x at 25 Hz for 15s ...
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bioRxiv - Cancer Biology 2022Quote: A 100 µL slurry of 5% Ni-NTA magnetic beads (Qiagen, part# 36113) was placed in 12 1.5 mL Eppendorf tubes and the tubes were placed in a magnetic stand and the supernatant was removed ...
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bioRxiv - Microbiology 2022Quote: ... (5) hybrid libraries were then eluted in 40μL of elution buffer (Qiagen, 19086).
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bioRxiv - Microbiology 2023Quote: ... or a 1.5 ml tube with 5 volumes of RNAlater (Qiagen, Valencia, CA) and preserved at -80 °C for RT-PCR.
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bioRxiv - Microbiology 2023Quote: ... containing 500 µl PBS and one 5 mm steel bead (Qiagen, Hilden, Germany) prior to bead-beating for 20 seconds to disrupt the cuticle ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was loaded onto a 5 mL StrepTactin Superflow Plus catridge (Qiagen) using a 1 mL/min flow rate and washed with Cyclin B1 wash buffer and eluted with wash buffer containing 2 mM desthiobiotin ...
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bioRxiv - Molecular Biology 2024Quote: Gcn4 and Med15 supernatants were mixed with 5 ml Ni-NTA beads (Qiagen) and incubated at 4°C for 1 hour ...
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bioRxiv - Physiology 2024Quote: ... and 5 μl of QuantiFast SYBR Green RT-PCR Master Mix (Qiagen, Germany). Reverse transcription was initiated with a hold at 50 °C for 10 min (cDNA synthesis) ...
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bioRxiv - Biochemistry 2024Quote: ... the supernatant was applied to 5 ml of Ni-NTA agarose beads (Qiagen). The beads were washed with 200 ml of wash buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... and TY1C2 were mixed with 5 μg/ml of RNase A (19101, QIAGEN) and 1 mg/ml of Proteinase K (EO0491 ...
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bioRxiv - Cancer Biology 2021Quote: ... cfDNA was extracted from approximately 4□ml of plasma (QIAamp Circulating Nucleic Acid kit, Qiagen) and then constructed into sequencing libraries with end repair ...
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bioRxiv - Genomics 2020Quote: Testis tissue was homogenized at 4°C in 1ml QIAzol Lysis Reagent (Qiagen, Hilden, Germany) together with RNase-Free Zirconium Oxide Beads (NextAdvance ...
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bioRxiv - Genomics 2019Quote: ... cfDNA was extracted from 1 or 4 ml of urine according to manufacturer recommendations (Qiagen Circulating Nucleic Acid Kit ...
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bioRxiv - Biophysics 2020Quote: ... 4 °C) and the supernatant was then incubated with 200 µl Ni-NTA (#30230, Qiagen) beads for 1 hour at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... 4 μl of PCR products were subsequently added to 21 μl PyroMark Master Mix (Qiagen) containing 10 pmol of barcoded primers (adapted from NEXTflexTM 16S V1-V3 Amplicon Seq Kit ...