Labshake search
Citations for Qiagen :
1501 - 1550 of 4034 citations for 7 trifluoromethyl 1 2 3 4 tetrahydro 5 nitroisoquinoline hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Single cells of Desulfovibrio or Methanococcus were sorted into wells of a 96-well plate containing 3 µl of PBS and Buffer D2 from Repli-G single cell kit (Qiagen) by using Influx flow cytometer (BD) ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: 3-10k cells were FACS sorted into buffer RLT with β-mercaptoethanol and total RNA prepared using RNAeasy Micro (Qiagen) with on column DNase I treatment ...
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bioRxiv - Immunology 2019Quote: For every qPCR assay a total of 3 biological samples (12 larvae each) were collected in QIAzol lysis reagent (Qiagen) and RNA was extracted using the miRNeasy mini kit (Qiagen ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from the rosette leaves of healthy 3-week-old plants (before yellow necrotic lesions develop) using the RNeasy Plant Mini Kit (Qiagen). All results shown include data from three biological replicates ...
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bioRxiv - Plant Biology 2019Quote: ... RNA for ISO-seq was extracted from cells grown for 3 days in mating inducing media (Sørensen et al., 2014) using the RNeasy Mini kit (Qiagen, USA). Nuclei were isolated from the cell pellets (Raimundo et al. ...
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bioRxiv - Immunology 2021Quote: ... Each AES was then amplified by PCR on a 3 kb fragment using primer X5 and X6 and purified using QIAquick PCR Purification kit (Qiagen). For gene yrbE4A ...
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bioRxiv - Immunology 2021Quote: Total RNA extracted from lungs on dpi 3 was reverse transcribed as described above and was mixed with RT2 SYBR Green ROX qPCR Mastermix (Qiagen) to perform RT² Profiler™ PCR Array Mouse Hypoxia Signaling Pathway (Qiagen ...
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Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... The temperature was increased from 25 °C to 95 °C at 3 °C per minute and fluorescence was measured at 610 nm in a Rotor-Gene Q thermocycler (Qiagen). The experiment was performed in technical triplicates ...
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bioRxiv - Neuroscience 2019Quote: ... The genomic region flanking the CRISPR cleavage site was amplified by PCR using specific Surv-F and Surv-R primers to amplify 900 bp surrounding the genomic cleavage site (Supplementary Table 3) and the resulting products were purified using PCR Cleanup kit (Qiagen). Cleavage assays were performed using 400ng of PCR products that were denatured by heating at 95°C then reannealed to form heteroduplex DNA using a thermocycler ...
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bioRxiv - Plant Biology 2020Quote: ... and LIC2 adaptor at 3’(5’-gA ggA gAA gAg CCg Tcg-3’) by overhang PCR amplification and purified by gel-extraction using QIAquick Gel Extraction Kit (Qiagen). Then ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... Transcripts were stabilized by mixing 3 mL of cell cultures at the mid-log phase with 6 mL of RNAprotect Bacteria Reagent (Qiagen). Samples were immediately vortexed for 5 sec ...
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bioRxiv - Systems Biology 2020Quote: ... For extraction of RNA from frozen liver the Qiagen TissueLyser instrument with 3 mm stainless steel beads and the RNeasy Mini Kit (Qiagen) was used ...
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bioRxiv - Microbiology 2021Quote: ... and RNA was isolated from 3 biological replicates of each strain when the cultures had reached OD600 = 0.1 using the RNeasy mini kit (Qiagen #74106). RNA-Seq was performed at the Genomics Core Facility at Princeton University ...
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bioRxiv - Neuroscience 2020Quote: ... Genomic DNA from ∼250,000 neurons per sample (n = 3 samples per group) was extracted and purified (DNeasy Blood and Tissue DNA extraction kit, Qiagen) prior to RRBS (Ovation RRBS Methyl-Seq System ...
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bioRxiv - Microbiology 2020Quote: ... and supplemented with 1X EDTA and 1X Halt Protease inhibitor cocktail (Thermo-Scientific, CAT No.:78440) and were lysed using bead beating (50 Hz for 3 five-minute cycles, TissueLyzer II (Qiagen) with 0.1 mm silica beads) ...
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bioRxiv - Cell Biology 2022Quote: ... each sample was from a separate NRVM isolation from a total of 3 isolations) and homogenized in RLT buffer (Qiagen) using green RINO homogenization tubes following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: 400–1000 ng partial fragments of tRNA-Tyr were digested in 3 μl aliquot with 20 ng RNase A (QIAGEN) in 10 mM NH40Ac pH 7 ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA was isolated from 3×106 cells by spin-column based DNA purification according to the manufacturer instruction (DNeasy, Qiagen). Knockout efficiency was analyzed on the genomic level through TIDE analysis (Tracking of Indels by DEcomposition) ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from single-cell clone or bulk GFP+ and GFP- cells (n = 3 with cells from three different mice in each group) using QIAzol lysis reagent (Qiagen). DNA was removed using the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Neuroscience 2019Quote: ... was extracted from P7 dissected neocortices separated from meninges of Ctrl and Nr2f1cKO mice (n=3) using the RNeasy Mini kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and were ligated by PCR using primers Lpg499-DR and Lpg499-UF to generate a 3-kb fragment that was purified by a gel extraction kit (Qiagen). The purified 3-kb fragment was introduced into the KS79 strain by natural transformation (76 ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from control and INPP5ED477N/D477N organoids (n=3 samples per genotype) using an RNeasy Plus Micro Kit (Qiagen) and reverse transcribed using Superscript™ IV VILO™ Master ezDNase enzyme (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA was isolated from the rosette leaves (5th and 9th) of healthy 3-week-old plants using the RNeasy Plant Mini Kit (Qiagen) including DNase I treatment ...
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bioRxiv - Neuroscience 2021Quote: ... day 10 embryoid bodies (EBs) and D45-47 cortical organoids (3 pooled EBs or COs per sample) using RNeasy Mini kit (Qiagen). Approximately 0.5-1ug of RNA was used as input to generate cDNA using Superscript IV Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were washed with PBS to remove as much media as possible prior to resuspension in 3 mL RNA Protect Bacteria Reagent (Qiagen). After incubating for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... Amplified DNA was run on a gel and a band the length of Nectin-3 (∼1800 bp) was cut from the gel and purified (Qiaquick Gel Extraction Kit, Qiagen). Sequences were then amplified (KOD hot start DNA polymerase ...
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bioRxiv - Immunology 2022Quote: ... A549-ORF7a and A549-ORF7b cells were seeded (3×10E5) in 6-well plates and lysed using RLT buffer for RNA isolation (RNeasy mini kit, Qiagen). Each sample was performed in triplicate ...
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bioRxiv - Molecular Biology 2019Quote: ... the plasmid DNA of mcr-1-producing Escherichia fergusonii (ICDC-ZG2016M34-3) which was acted as a representative sample for optimization of reaction condition and sensitivity detection was acquired by QIAGEN Plasmid Kits ...
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bioRxiv - Microbiology 2019Quote: RNA was harvested from late-log phase Mtb and the 3’ end of Mcr11 was mapped using the miScript Reverse Transcription Kit (Qiagen) (88 ...
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bioRxiv - Microbiology 2020Quote: ... templates containing a T7 phage promoter sequence (5’-TAATACGACTCACTATAGGG-3’) were amplified by PCR using corresponding primer pairs and were purified with a QIAquick PCR purification kit (Qiagen). The primers used are listed in S1 Table ...
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bioRxiv - Cell Biology 2019Quote: The indicated tissues and SSC were isolated from Mmp14wt/wt and Mmp14Y573D/Y573 mice (3 mice/tissue/genotype) and total RNA was extracted using the RNeasy kit (Qiagen). The subsequent steps were carried out by personnel of the Genome Technology Center of NYU School of Medicine ...
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bioRxiv - Developmental Biology 2020Quote: E9.5 hindbrain spanning rhombomeres 1-6 were dissected from wild type and mutant embryos (n=3) to obtain total mRNA preparations (miRNeasy Micro Kit, Qiagen). Sequencing libraries were prepared following the SMART-Seq v4 Ultra Low Input RNA (TaKaRa ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Immunology 2021Quote: ... genomic DNA was extracted from cells 3 d after electroporation using a QIAamp DNA Mini Kit or Micro Kit (QIAGEN) per the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T and Neuro2a cells were harvested 3 days post-transfection and genomic DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... total RNA was isolated from 0.4×106 FACS-sorted B220hi CD138- and B220low CD138+ B cells (derived from 3 biological replicates after eight days in culture) using the RNeasy Mini Kit (Qiagen). cDNA was generated from polyadenylated transcripts employing the SMART-Seq v4 ultra low input RNA kit (Takara Bio) ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was extracted from edited and unedited cells 3 days post nucleofection using the DNeasy Blood and Tissue Kit (Qiagen). PCRs were performed with primers spanning the gRNA binding site (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures that were cultivated on one filter membrane (3 cultures) were transferred as one sample into RLT buffer (QIAGEN) and RNA was isolated according to the manufacturer’s instructions (RNeasy Plus Micro Kit ...
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bioRxiv - Biophysics 2022Quote: ... The liquid culture was then grown for 3 hours at 37°C before the plasmid extraction using a miniprep kit (QIAGEN). Prepared DNA was sequenced by the Microbial Genome Sequencing Center (https://www.seqcenter.com/ ...
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bioRxiv - Cell Biology 2022Quote: RNA was extracted from cryo-pulverized inguinal adipose tissue from freely-fed 14-day-old mice (n=3 male and female for PTPN23H/H and PTPN23+/+) using the RNeasy Mini Kit (74104; Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... genomic DNA was isolated 3 or 8 days after editing by a QIAamp DNA Mini Kit or Micro Kit (QIAGEN). The genomic region flanking the CRISPR/Cas9 cleavage site was PCR-amplified ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed 3 times with PBS to eliminate unbound FVVs and RNAs were extracted using RNeasy plus mini Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... The process was repeated twice (3 in total) and the leukocyte enriched pellet was lysed in 350 µl RLT buffer (Qiagen) and stored at −20 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The tissue was then ground to a fine powder using 3/16 inch (4.76mm) ball bearings in a Tissuelyzer II (Qiagen, Germany) in the presence of dry ice in the pockets around tube holders ...
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bioRxiv - Plant Biology 2023Quote: ... the resulting dried leaves were then ground to powder with a 3-mm glass bead placed in a tube with a Tissuelyser mill (Qiagen). The ground samples were incubated at 4°C in DNA extraction buffer (200 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 20 ovaries were dissected from 3-day-old adult females and DNA extraction was performed using the Gentra Puregene Blood and Tissue kit (Qiagen). DNA samples used for PacBio sequencing were produced using the protocol described in (Ellegaard et al. ...
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bioRxiv - Neuroscience 2024Quote: Genomic DNA was isolated from ipsilateral and contralateral ventral midbrain hemispheres at 3 months after AAV-GFP or AAV-Cre-GFP injections using the DNeasy Blood and Tissue kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... tissues were placed in 300μL of sterile PBS containing sterile glass beads and mechanically lysed at a frequency of 20 shakes per seconds for 3 minutes in a TissueLyser II (Qiagen). Negative controls consisted of tubes containing PBS and beads but no sample ...
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bioRxiv - Microbiology 2024Quote: ... mosquitoes were homogenized in 500 μl ice-cold 1X Phosphate Buffered Saline buffer with two ice-cold steel bearing balls (3 mm diameter, LOUDET) using a TissueLyser II (Qiagen) and clarified through centrifugation ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was isolated from untreated or RNP-transfected plerixafor-mobilized HD and SCD HSPCs (n=3 for each group) using the RNeasy Kit (QIAGEN) that includes a DNAse treatment step ...