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Citations for Qiagen :
1451 - 1500 of 1826 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Phoenix-Eco or Phoenix-Eco alpha-V beta-3 were transfected with LZRS-vector constructs using Effectene transfection reagent (Qiagen, 301425) as follows ...
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bioRxiv - Bioengineering 2019Quote: ... The bacterial pellet was processed to purify all containing DNA by alkaline lysis and column purification (Qiagen miniprep spin kit, Qiagen, Inc., Germany).
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bioRxiv - Microbiology 2019Quote: ... The resulting 11.4kb HIV vector-containing fragments free of plasmid backbone were purified from agarose using QIAquick Gel Extraction Kit (Cat No./ID: 28706 Qiagen, Germantown, MD). A 304 bp zip code-containing insert fragment pool was generated by PCR using NL4-3 GPP or GPV- as template ...
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bioRxiv - Bioengineering 2020Quote: ... the resulting supernatant containing soluble LCC was subjected to purification by immobilized metal ion chromatography (IMAC) using Ni-NTA Superflow (Qiagen, Hilden, Germany). Protein elution using 250 mM imidazole was then dialyzed against 100 mM potassium phosphate buffer (pH 8 ...
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bioRxiv - Molecular Biology 2019Quote: The genomic DNA was extracted from the blood and the microfilaria-containing tissues using the QIAGEN DNA tissues extraction kit (QIAGEN, Hilden, Germany), following the manufacturer’s recommendations ...
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bioRxiv - Physiology 2020Quote: ... existing media was removed and the resulting myotubes received either 750 µl of fresh DM alone or 750 µl DM containing 9 µl HiPerFectTransfection™ (Qiagen, UK) and 20 nmol Flexitube GeneSolutions™ (siUBR5 ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged proteins were recovered from the clarified lysate using a drip column containing a 5.0 ml bed volume of Ni-NTA resin (Qiagen, Mississauga, ON, Canada). After extensive washing with buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... A final 370,000 x g centrifugation at 4°C for 45 min yielded a myosin-containing supernatant that was loaded onto a 1 mL nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Cat# 30210) column equilibrated with column buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1,000 T-cells/CAR T-cells were FACS-sorted in single PCR tubes containing 4 ul of QIAGEN lysis buffer from QIAseq FX Single Cell RNA Library Kit (QIAGEN, Hilden, Germany) and stored at −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and areas containing >30% tumor cells as determined by an expert pathologist were macrodissected and deparaffinated using Deparaffinization Solution (Qiagen, Hilden, Germany). DNA was extracted and purified with GeneRead DNA FFPE Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Genetics 2022Quote: ... The PCR amplification was carried out in a 10-μl reaction volume containing 8 μl of Taq PCR Master Mix (Qiagen, Hilden, Germany), 10 μM of each primer (1 μl) ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampus were dissected and either quick frozen on dry ice for processing for western blotting or placed into tubes containing RNAlater (Qiagen; Hilden, Germany) for RNA extraction and stored at −80°C until further processing.
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bioRxiv - Developmental Biology 2019Quote: ... the intestines were transferred to autoclaved Eppendorf tubes containing 350μL of RLT Buffer plus from the RNeasy Plus Micro Kit (QIAGEN Cat. No 74034) with 1% v/v of 2-mercaptoethanol (Sigma-Aldrich M6250) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was extracted from cells grown in M9 media containing pyruvate (0.4% w/v) as sole carbon source by column capture (Qiagen RNeasy Mini Kit). Purified RNA was subjected to additional DNase treatment (Turbo™ DNase ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Genetics 2023Quote: ... amplifications were performed in reaction volumes of 25 μL using a reaction mix containing 1x Type-it Multiplex PCR Master Mix (Qiagen, Gmbh, Hilden), 0.2 μM of each forward and reverse primer pair ...
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bioRxiv - Genetics 2023Quote: ... Frozen tissue samples were homogenised by adding 600 uL RLT containing 1% β-Mercaptoethanol and a 5mm stainless steel bead (Qiagen # 69989) and processing for 40 seconds at 15 Hz with the TissueLyser II ...
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bioRxiv - Immunology 2023Quote: ... Spleens were harvested at indicated timepoints and homogenized in a 2ml homogenizer tube (Fisher Brand Cat. 14-666-315) containing 1ml sterile PBS and one 5mm stainless steel bead (QIAGEN Cat. 69989). For oral infection ...
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bioRxiv - Molecular Biology 2024Quote: ... We typically dissected about 20 slices per HVC and transferred the tissue into an Eppendorf tube containing 340 μl of RLT buffer mixture (with DTT, Qiagen, Valencia, California). We used these rather thin sections for dissection of HVC to avoid contamination of the samples with non-HVC tissues ...
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bioRxiv - Evolutionary Biology 2021Quote: Protein sequence alignments and tree production were made using the CLC main work bench (ver. 5.5.1, Qiagen) software package ...
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bioRxiv - Physiology 2019Quote: ... RNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit according to manufacturer’s instructions (Qiagen, 80004). RNA quality was determined by Agilent 2200 TapeStation and quantified by Qubit (Life Technologies) ...
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bioRxiv - Plant Biology 2019Quote: Sitting-drop vapour-diffusion experiments identified suitable crystallisation conditions for native protein from the PACT screen (QIAGEN), with optimisation identifying the best conditions for crystal formation as 6 mg/mL protein combined 1:1 with 8% PEG 1500 ...
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bioRxiv - Microbiology 2019Quote: ... The DNA from the appendix samples was obtained using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN), before the DNA extraction ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nic96186-839-VHH-SAN12 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 8% (w/v) PEG 8,000 and 0.1 M tri-sodium citrate pH 5.0 (Protein Complex suite, Qiagen). Hanging drops of 1 ml protein at 6mg/ml and 1 ml of precipitant (7-10% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... and His-tagged protein was purified from cell lysate using a Ni-NTA spin column (Qiagen, 31014). The resulting product was run on a 12% Bis-Tris PAGE gel (Thermofisher ...
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bioRxiv - Molecular Biology 2020Quote: Nme2Cas9 protein was expressed in Rosetta (DE3) cells and purified using a Ni2+-NTA agarose column (QIAGEN) as previously described24 ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2 mM TCEP) the expressed protein was extracted from the supernatant using NiNTA resin (QIAGEN, Germantown, MD). The resulting eluate from the NiNTA resin using lysis buffer supplemented with 250 mM Imidazole pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 6xHis tagged protein were purified by immobilized metal ion chromatography using nickel-NTA super flow resin (Qiagen) as previously described with slight modifications46 ...
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bioRxiv - Plant Biology 2021Quote: Alignments of histone DNA and protein sequences was performed using CLC Main Workbench software version 8.1 (QIAGEN). Predictions of intrinsic disorder were undertaken by PONDR (58) ...
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bioRxiv - Immunology 2022Quote: ... https://www.beiresources.org/).Protein was purified using gravity flow purification with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen, Germany) and concentrated and buffer exchanged in Amicon centrifugal units (EMD Millipore ...
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bioRxiv - Immunology 2019Quote: His-tag purified recombinant proteins were expressed in E.coli and purified by Ni+-NTA-agarose column (Qiagen). Then ...
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bioRxiv - Biochemistry 2019Quote: ... Expressed protein was purified by elution from a nickel-nitrilotriacetic acid agarose (Ni-NTA) (Qiagen, Germantown, MD) column with 0.5 M imidazole and buffer exchanged into TEV buffer (25 mM NaHPO4 ...
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bioRxiv - Genomics 2021Quote: ... RNA-protein complexes were then incubated with Ni-NTA sepharose (Qiagen, 100 μl of slurry per sample) overnight at 4°C and extensively washed ...
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bioRxiv - Developmental Biology 2020Quote: ... The significantly changed proteins were used as inputs for Ingenuity Pathway Analysis (IPA, Version 03-13, Qiagen). The IPA predicted the altered pathways ...
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bioRxiv - Biochemistry 2021Quote: ... All the protein samples were expressed in BL21(DE3) and purified using Ni-NTA Sepharose column (QIAGEN) and Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... The polyhistidine-tagged recombinant proteins were then purified from bacterial lysates with Ni2+-NTA agarose beads (QIAGEN) and washed by three different washing buffers (Washing buffer 1 contained 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA were isolated from equivalent amounts of HDL total protein using the miRNeasy Mini Kit (Qiagen). For sRNA analyses ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... 0.2 µg of AFP1-His proteins was incubated with 20 µl Ni-NTA agarose beads (Qiagen, Germany) in buffer (25 mM Tris-Cl ...
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bioRxiv - Immunology 2020Quote: ... HIS-tagged DR protein monomers were produced by Hi5 insect cells and purified using Ni-NTA (Qiagen) and size exclusion columns (AKTA ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was isolated using the All prep bacterial DNA/RNA/protein kit following the manufacturer’s (Qiagen) protocol ...
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bioRxiv - Microbiology 2022Quote: ... The biomolecular extraction was then performed using the commercial AllPrep DNA/RNA/Protein Mini Kit (Qiagen-80004), conducted on a customised robotic system owned by the lab (Tecan-LU_UNILU_EWS_EXTRACTION_EU-0908-Freedom EVO 200) ...
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bioRxiv - Plant Biology 2023Quote: ... The His-ZmTPL2N-His recombinant protein was purified using Pierce Ni-NTA resin (QIAGEN, Cat. No. 30210) according to the product protocol ...
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bioRxiv - Immunology 2023Quote: ... Protein exaction from the deparaffinised sample was carried out using the Qiagen Qproteome kit (Qiagen, Hilden, Germany) as per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... CBS proteins with a TEV cleavable N-terminal His-tag were purified using Ni-NTA agarose (Qiagen) resin and were treated to gel filtration using a Superose 6 Increase 16/600 column or Superdex 200 Hiload 16/600 column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from all the cell lines using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). The total RNA was then treated with Turbo DNA-free kit (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: Melting curves of proteins were measured using the Rotor-Gene Q real-time PCR cycler (Qiagen, Germany). 25 μl samples with a protein concentration of approximately 0.5 mg/ml in a buffer containing 100 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA for each Klebsiella strain was extracted using the AllPrep Bacterial DNA/RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... supernatants were harvested and His-tagged tIgM-Fc (tFcµ) protein was purified using Ni-NTA Agarose (Qiagen) and subsequently ...