Labshake search
Citations for Qiagen :
1451 - 1500 of 10000+ citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... The supernatant was 0.2 µM filtered and was incubated overnight at 4°C with 5 mL of Ni-NTA beads (Qiagen). XXX
-
bioRxiv - Cell Biology 2022Quote: ... The tagmentation reaction mixture was transferred in a 1.5ml low-binding tube and incubated at 37°C for 30 min and stopped by the addition of 250μl Buffer PB (Qiagen). The tagmented chromatin was purified using the MinElute PCR purification kit (Qiagen 28004) ...
-
Altered motility in response to iron-limitation is regulated by lpdA in uropathogenic E. coli CFT073bioRxiv - Microbiology 2023Quote: ... Strains were cultured for five hours at 37°C with aeration before harvest and treatment with bacterial RNAprotect (Qiagen). Bacterial pellets were stored at -80°C until RNA isolation.
-
bioRxiv - Bioengineering 2024Quote: Ten millilitres of culture were pelleted by centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
-
bioRxiv - Microbiology 2023Quote: ... The soluble lysates obtained after two rounds of centrifugation at 18,000g and 4°C were incubated with glutathione agarose beads (Pierce) or Ni2+-NTA beads (QIAGEN). Recombinant proteins were eluted from the beads using 10 mM reduced glutathione in a Tris (50 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2024Quote: ... The culture was then combined with LB + 25 µg/mL chloramphenicol + 0.2% glucose (w/v) culture for overnight growth (37 °C) and then miniprepped (Qiagen).
-
bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The soluble lysate obtained after centrifugation at 13,000 rpm for 30 minutes at 4°C was incubated with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... DNA on the exterior of filtered capsids was digested for 1 h at 25°C with 20.3 units DNase (79254; Qiagen) supplemented with 10 mM MgCl2 ...
-
bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified with a DNA gel extraction kit (QIAquick Gel Extraction Kit, Qiagen) and used for library preparation with TruSeq/ChIP-Seq reagents (Illumina) ...
-
bioRxiv - Developmental Biology 2021Quote: ... Total RNA isolation was then carried out using a RNA extraction Kit (RNeasy Mini Kit, Qiagen). scRNA-seq was performed using the Smart-seq2 protocol68 ...
-
bioRxiv - Molecular Biology 2020Quote: ... total DNA was purified from supernatant using commercially available kit (DNeasy 96 Blood & Tissue kit, Qiagen). The HBV DNA was amplified in a real-time PCR assay using LightCycler 480 (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: ... The RNA was purified with the PeqGOLD Total RNA Kit (PeqLab) or RNeasy Mini Kit (Qiagen) following the manufacturer’s instruction and an additional DNAse I digestion was performed on the column provided in the kit ...
-
bioRxiv - Microbiology 2019Quote: ... using the QIAamp Viral RNA Mini Kit or QIAamp Circulating Nucleic Acid kit (Qiagen, Hilden, Germany). Total nucleic acid was eluted in two centrifugation steps with 40 µl of Buffer AVE each ...
-
bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were purified using the QIAprep Spin Miniprep Kit or the HiSpeed Plasmid Maxi Kit (QIAGEN). Nucleotide sequences of all cloned constructs were confirmed by DNA sequencing (Eurofins Genomics) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... RNA eluate was purified with the RNeasy RNA clean up kit RNeasy Mini Kit (Qiagen, NL) as per protocol ...
-
bioRxiv - Genetics 2020Quote: ... dhfr products were excised from the gel using commercial kits (QIAquick Gel Extraction Kit, Qiagen, Germany). 20 μl of eluted DNA was then purified using AMPure XP Magnetic Beads (1X ...
-
bioRxiv - Biochemistry 2020Quote: Total RNA was prepared from frozen plant material using plant RNA purification kit (QIAGEN RNeasy Kit). Following electrophoresis (1.5%(w/v ...
-
bioRxiv - Microbiology 2021Quote: ... The QIAquick Gel Extraction Kit and the QIAquick PCR Purification Kit (QIAGEN Inc, Valencia, CA, USA) were used for PCR- product clean-up ...
-
bioRxiv - Cell Biology 2020Quote: ... and was retro-transcribed using the Qiagen reverse transcription kit (miScript II RT Kit, #218161, Qiagen) and pre-amplified using miScript PreAMP PCR Kit (# 331451 ...
-
bioRxiv - Bioengineering 2022Quote: RNeasy Plus Mini Kits for RNA extraction and QuantiTect Reverse Transcriptase Kits were purchased from Qiagen. β-mercaptoethanol was purchased from Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... and then the total RNA was extracted using an RNA isolation kit (RNeasy Mini Kit, Qiagen). Ribosomal RNA was depleted using Ribo-ZERO magnetic kit (Epicentre) ...
-
bioRxiv - Microbiology 2019Quote: ... RNA was extracted with the RNeasy Mini Kit (tissues) or Viral RNA Mini Kit (serum) (Qiagen). ZIKV RNA levels were determined by TaqMan one-step quantitative reverse transcription PCR (qRT-PCR ...
-
bioRxiv - Microbiology 2019Quote: ... This was preferred over rapid gDNA isolation kits such as the DNeasy Blood & Tissue kit (Qiagen), as the latter isolation kit is strongly biased towards shorter DNA fragments (predominantly 30kb in length compared to up to 150kb for the 100/G columns ...
-
bioRxiv - Bioengineering 2019Quote: RNA was extracted from cells using the RNeasy Mini Kit or RNeasy plus Micro Kit (Qiagen) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... Whole genome amplification (WGA) kit (REPLI-g® Mini Kit) was obtained from Qiagen (Venlo, Netherlands). Tryptic soy broth (TSB ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was extracted from cells harvested by trypsin with RNA extraction kit (Qiagen, RNeasy Mini Kit), and each group was done in duplicates ...
-
bioRxiv - Cancer Biology 2022Quote: ... and lysed using the Qiagen RNA Extraction Kit (RNEasy kit, catalog no. 74104, Qiagen, Hilden, Germany) following the recommended protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... RNeasy Plus Mini Kits for RNA extraction and QuantiTect Reverse Transcriptase Kits were purchased from Qiagen. Roche Light Cycler-DNA Master SYBR Green I mastermix and Corning Axygen 384-well PCR microplates were purchased through the MIT BioMicro Center / KI Genomics Core ...
-
bioRxiv - Microbiology 2023Quote: ... gDNA was extracted using an isopropanol-ethanol purification kit (Puregene Yeast/Bact. Kit B from Qiagen) and following the manufacturer’s protocol including a 60-minute RNase A treatment ...
-
bioRxiv - Genomics 2023Quote: ... The DNA was further purified using the Blood & Cell Culture DNA Midi Kit (Qiagen kit #13343) following the manufacturer’s protocol after one hour of protease digestion ...
-
bioRxiv - Immunology 2023Quote: ... RNA was isolated from single cell suspensions using a commercial kit (Qiagen RNeasy Mini Kit, Qiagen) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2023Quote: ... RNA from each strain was extracted using a commercial kit (miRNeasy Mini Kit, Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... SDS and an RNA/DNA All-prep kit: DNeasy Blood and Tissue kit (Qiagen, Valencia, CA). DNA for 16S rRNA sequencing was processed using the Earth Microbiome Project protocols (28,41–44 ...
-
bioRxiv - Bioengineering 2023Quote: ... Total RNA was prepared using an RNA purification kit (RNeasy Mini kit; Qiagen Inc., Valencia, CA). The isolated RNA was reverse transcribed using the PrimeScript™ RT reagent kit (Takara Bio Inc.) ...
-
bioRxiv - Genomics 2023Quote: ... All extractions were made using a commercial kit (RNeasy Plus Mini Kit; Qiagen, Toronto, ON, Canada) with quality control being determined using a combination of a Nanodrop (260/280 & 260/230 ratios ...
-
bioRxiv - Immunology 2023Quote: ... RNA was isolated from single cell suspensions using a commercial kit (Qiagen RNeasy Mini Kit, Qiagen) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... RNeasy Mini Kit (cat#74104) and QIAprep Spin Miniprep Kit (cat# 27106) were bought from Qiagen. The scrather was purchased from SPL life sciences ...
-
bioRxiv - Genetics 2024Quote: ... All PCR products were purified using QIAquick PCR Purification Kits or MinElute PCR Purification Kits (QIAGEN). Plasmid and genomic sequences were verified through Sanger sequencing (Eurofins Genomics ...
-
bioRxiv - Genetics 2024Quote: ... and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN). All PCR products were purified using QIAquick PCR Purification Kits or MinElute PCR Purification Kits (QIAGEN) ...
-
bioRxiv - Microbiology 2024Quote: ... and six DNA extraction kit controls using the DNeasy PowerSoil Pro Kit (Qiagen®, Hilden, Germany) and following the manufacturer’s instructions with a few modifications as described below ...
-
bioRxiv - Plant Biology 2020Quote: ... MiniElute PCR Purification Kit (Qiagen, Germany) was used for cleaning ChIP DNA and two rounds of in vitro transcription by T7 RNA polymerase followed according to a linear DNA amplification (LinDA ...
-
bioRxiv - Neuroscience 2021Quote: ... the RNeasy Mini kit (Qiagen, 74104) was used and cells were directly lysed in the provided RLT lysis buffer in the cell culture plate ...
-
bioRxiv - Cancer Biology 2021Quote: ... using the RNeasy Mini kit (Qiagen). Purified RNA was reverse transcribed to cDNA using Transcriptor Reverse Transcriptase (Roche) ...
-
bioRxiv - Microbiology 2020Quote: ... QIAquick Gel Extraction Kit (Qiagen, UK) following the instructions of the manufacturer ...
-
Huge and variable diversity of episymbiotic CPR bacteria and DPANN archaea in groundwater ecosystemsbioRxiv - Microbiology 2020Quote: ... solution C1 (Qiagen DNAeasy PowerSoil Kit) was added to the Powerbead solution ...
-
bioRxiv - Microbiology 2020Quote: ... and QIAamp DNA Mini Kit (Qiagen) were used for plasmid preparations and H ...
-
bioRxiv - Cancer Biology 2021Quote: ... or miScript II RT Kit (Qiagen). Noteworthy ...
-
bioRxiv - Cell Biology 2020Quote: ... using the RNeasy Kit (Qiagen # 74104) following the manufacturer’s instructions including the on-column DNase I digestion (Qiagen #79254).