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Citations for Qiagen :
1451 - 1500 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Next RNA and protein were digested and DNA was recovered by Qiagen PCR purification kit ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellets were then resuspended in 90 μl Buffer ALO (Qiagen) supplemented with 0.4X NuPAGE™ LDS Sample Buffer (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged protein was purified on a 1ml Ni-NTA column (Qiagen) prewashed in buffer A containing 500mM imidazole w/o β−mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were then purified with Ni-NTA agarose beads (QIAGEN; 30210) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... His10 proteins were purified by using Ni-NTA agarose (QIAGEN, Catalog # 30230), followed by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The 6x-His-tagged proteins were captured using Ni-NTA Superflow (QIAGEN) and the His-tag was cleaved at 4 °C for 16-18 hours by human thrombin (Biopharm laboratories) ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were purified using Ni2+ affinity chromatography (Ni-NTA agarose, Qiagen) and then passed over an anion-exchange column (Hitrap Q HP ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was purified by affinity chromatography using Ni-NTA agarose (Qiagen) resin followed by his tag cleavage by TEV protease and gel filtration using Superdex 200 Increase 10/300 GL column (G.E ...
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bioRxiv - Neuroscience 2024Quote: ... The fusion protein was purified using Ni-NTA Resin Spin Columns (QIAGEN). Eurogentec (Belgium ...
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bioRxiv - Genomics 2021Quote: ... DNeasy Blood & Tissue Kit and QIAamp DNA FFPE Tissue Kit (Qiagen) were used for Genomic DNA extraction of paired FF and FFPE samples respectively.
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bioRxiv - Genomics 2021Quote: ... These kits included QIAamp Circulating Nucleic Acid Kit (Qiagen, Hilden, Germany), Plasma/Serum Cell-Free Circulating DNA Purification Mini Kit (Norgen Biotek ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plasmid Midiprep kit and Maxi kit were from QIAGEN (Valencia, CA). The 1,4-dioxane ...
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bioRxiv - Neuroscience 2023Quote: ... The cDNA was synthesized using the KIT Quantitec Transcription KIT (QIAGEN) as recommended by the manufacturer ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted with kits from QIAGEN (MagAttract HMW kit) and MACHEREY-NAGEL (Nucleospin Tissue Kit) ...
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bioRxiv - Microbiology 2024Quote: ... according to kit instructions (REPLI-g single cell kit, #150345, QIAGEN). Sequencing libraries (PE150 ...
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bioRxiv - Bioengineering 2024Quote: ... RNA extraction kit and reverse transcription kit was obtained from Qiagen.
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated for 45min at 42°C and the reaction purified with 15µl of MagAttract beads (Qiagen). The labelled DNA was eluted with TE-buffer (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... snap frozen (−80°C) for proteomics analysis or dissolved in 300 ul of RNAlater reagent (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... tissues were incubated overnight at 56 °C in 300 μL of ATL digestion buffer (Qiagen®) including proteinase K (Cf = 1 mg/ mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were washed once with PBS (37 °C) and 350 µl RNA lysis buffer (Qiagen, Buffer RLT
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bioRxiv - Molecular Biology 2023Quote: ... Samples were stored at -80°C with the addition of RNA protect reagent (Qiagen, MD, USA). DNA was extracted using DNeasy PowerSoil kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN) (1 h at 50°C and then overnight at 37°C) ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were cultured for 16 hr at 37°C and DNA was prepared by maxiprep (Qiagen). The entire homology arm sequence and the whole assembled sequence was verified (Genewiz).
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bioRxiv - Neuroscience 2023Quote: ... DNA was eluted two times with 50 μl of pre-heated (70°C) EB (Qiagen, 80204) through centrifugation at 8,500×g for 1 min as documented previously10 ...
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bioRxiv - Neuroscience 2019Quote: ... Only the neocortical primordium and the commissural plate were dissected and stored in RNA Later (Qiagen) at −20°C until RNA extraction ...
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bioRxiv - Systems Biology 2020Quote: ... Each well in the NAB plate was then washed once with 500 uL Buffer AW1 (QIAGEN) and once with 500 μL of Buffer AW2 (QIAGEN) ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates in N2B27-2i and transfected with FlexiTube siRNAs (Qiagen) using DharmaFECT 1 (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and placed in a well of 96-well plate containing 2,5µl of RLT plus buffer (Qiagen). At the end of cell collection ...
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bioRxiv - Systems Biology 2023Quote: ... Each well in the NAB plate was then washed once with 500 μL buffer AW1 (QIAGEN) and once with 500 μL of buffer AW2 (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... Sodium bisulfite treatment was carried out using commercially available bisulfite conversion kits (EpiTect Fast Bisulfite Kit and EpiTect Fast LyseAll Bisulfite Kit; Qiagen) according to the manufacturer’s instructions except for the DNA denaturation step and sodium bisulfite reaction time ...
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bioRxiv - Microbiology 2024Quote: ... and the genomic DNA/RNA extractions were conducted using commercial extraction kits (DNeasy Blood & Tissue Kit for DNA or RNeasy Mini Kit for RNA, both from Qiagen) and purified with GeneJET Genomic DNA Purification Kit (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... total RNA was isolated using RNeasy Mini Kit or Micro Kit (Qiagen). Reverse transcription was performed using M-MLV Reverse Transcriptase and random primers following manufacturer’s instructions (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was extracted using a commercial kit (RNseasy Plant Mini Kit, Qiagen), followed by cDNA synthesis (iSCRIPT ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using standard column-based purification kit (QIAGEN RNeasy Kit, Cat. No. 74004). DNase treatment was applied during the purification to remove any potential DNA contamination ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted using the RNeasy Mini Kit extraction kit (QIAGEN). RNA-less and reverse transcriptase-less reactions were used as controls ...
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bioRxiv - Cancer Biology 2021Quote: ... purified with QIAquick Gel Extraction kit or QIAquick PCR Purification kit (Qiagen), ligated using T4 DNA Ligase (New England BioLabs) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were prepared using silica-membrane based kit (Plasmid Miniprep Kit, Qiagen) following the manufacturer’s instructions and quantified using Nanodrop Spectrophotometer ...
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bioRxiv - Genetics 2020Quote: ... according to the DNeasy Blood & Tissue kit and DNeasy Micro kit (QIAGEN). Total RNA was extracted from pelleted cells using the Rneasy Mini kit (QIAGEN ...
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bioRxiv - Microbiology 2019Quote: ... using viral RNA isolation kit (QIAmp Viral RNA Mini Kit Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and extracted with Jetgene mini prep kit (QIAprep Spin Miniprep Kit, QIAGEN). The correctness of constructions was verified by sequencing (Eurofins) ...