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Citations for Qiagen :
1451 - 1500 of 1510 citations for 1 Triisopropylsilyloxy cyclopropylmethanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... were weighed and homogenized in 1-10 µL/mg DMEM with a sterile glass bead at 30 Hz for 4 minutes using a TissueLyser (Qiagen, Germantown, MD) automated homogenizer ...
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bioRxiv - Cancer Biology 2023Quote: ... After incubations cells were pickup in buffer RLT with 1% of β-mercaptoethanol and total RNA purification proceeded following the instructions of RNeasy Plus mini kit (Qiagen, USA). Quality control of total RNA was carried out by spectrophotometric readings of optic density (OD ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from 1 mL of each human plasma sample using the DNeasy Blood and Tissue kit (Qiagen, 69504, Hilden, Germany) according to the manufacturer protocol and recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA sequencing on the pancreatic xenografts in the orthotopic mouse model using PDAC cells (AsPC-1 ± SERPINB3) was also performed following isolation of RNA with the RNeasy Plus Mini Kit (QIAGEN, Venlo, Netherlands). Sequencing was performed on NovaSeq 6000 SP (Illumina ...
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bioRxiv - Genomics 2024Quote: ... Total RNA was extracted from approximately 1 g of fresh gametophytic tissue using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA, USA).
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bioRxiv - Systems Biology 2024Quote: ... were transfected with one of three different commercial siRNA sequences designed to target the EGFR or an Allstars negative control siRNA sequence (final concentration 1 nM; all Qiagen Ltd., UK) using INTERFERin transfection reagent according to the manufacturer’s instructions (Polyplus Transfection ...
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bioRxiv - Biochemistry 2024Quote: ... Hippocampal lysates were prepared by the addition of ice-cold PBS (200 µL PBS/10 mg hippocampal wet weight) and homogenized for 1 min in a TissueLyser II instrument (Qiagen, Hilden, Germany). One fourth of the obtained volume of each lysate was used for the analysis of total PLP concentrations as described below ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred into the glovebox and loaded onto a gravity column in with 1 mL nickel-nitrilotriacetic acid resin (Qiagen, Hilden, Germany) that was preequilibrated with wash buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2024Quote: ... Frozen tissue samples were homogenised by adding 600 uL RLT containing 1% β-Mercaptoethanol and a 5mm stainless steel bead (Qiagen # 69989) and processing for 40 seconds at 15 Hz with the TissueLyser II ...
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bioRxiv - Neuroscience 2024Quote: Basal ganglia tissues were collected during necropsy in 1 cm3 cubes and processed for total RNA extraction using Qiazol (Qiagen Inc, CA). A detailed protocol is provided in the Supplemental methods ...
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bioRxiv - Molecular Biology 2024Quote: ... Products were resolved on a 1% (w/v) agarose gel and gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products was resolved in 1% agarose gel and the target band was gel purified using a QIAquick Gel Extraction kit (Qiagen, Hilden, Germany). The PCR products were subsequently subjected to direct Sanger sequencing using G680V-F to for the adjacent G680V – A684G SNPs and V528L Seq2 for the V528L SNP ...
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bioRxiv - Neuroscience 2024Quote: Eye tissue was suspended in Buffer RLT/eye (1 eye/mouse; 10-18 mice/genotype) and prepared using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was assessed by the 18S/28S rRNA bands in capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Bioengineering 2024Quote: ... cells were lysed in RLT buffer plus β-mercaptoethanol (1:100) and RNA was extracted using an RNeasy plus mini kit (Qiagen 74134). Reverse transcription into cDNA was performed according to iScript manufacturer instructions (iScript cDNA synthesis kit ...
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bioRxiv - Immunology 2024Quote: ... represents 104 sorted H-2Db-GP33-41-specific CD8+ T cells in RLT buffer with 1% ß-mercaptoethanol and processed with RNAeasy Micro Kit (Qiagen, 74004) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Fresh frozen tissue homogenates were prepared by thawing frozen tissue and placing 200 mg (± 50 mg) of minced tissue in a tube containing 1 ml DMEM culture medium and a steel bead (Qiagen, Germantown, MD, USA). Homogenization was performed with the TissueLyser LT (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of the template was used in a 25 μL reaction using the QuantiFast Probe RT-PCR Kit (Qiagen, Inc., Valencia, CA) with 0.4 μM of each primer and 0.2 μM probe ...
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bioRxiv - Genetics 2021Quote: Gene Ontology (GO) enrichment and network analysis of RNAseq results were performed using both Ingenuity Pathway Analysis (IPA) software package (Qiagen version 1-16) and manually developed excel pipeline workflows ...
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bioRxiv - Developmental Biology 2021Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaws were resuspended in 600 μl of RTL plus buffer supplemented with 1% β-mercaptoethanol (M3148-100ML, MilliporeSigma, Burlington, MA, USA) and Reagent DX (19088, Qiagen, Hilden, Germany). HH31 and HH34 lower jaws were processed in a Bead Mill 24 Homogenizer (15-340-163 ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were checked on 1% agarose gels before being purified using a QiaQuick gel extraction kit (Qiagen, Inc., Valencia, California, USA) and directly sequenced in both directions using the amplification primers on an ABI 3730 automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from the supernatant of Vero E6 cells after 1 passage using the QIAamp Viral RNA Mini kit (Qiagen, https://www.qiagen.com/us/) without addition of carrier RNA ...
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bioRxiv - Cell Biology 2021Quote: ... This was incubated in a 37 °C shaker for 1 hour before the addition of Proteinase K (10 µl, QIAGEN, >600 mAU / ml) and left at 55 °C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Lung homogenates were prepared by homogenizing 200 mg of tissue in a tube containing 1 mL DMEM and a steel bead with a TissueLyser LT (Qiagen, Germantown, MD, USA) for 30 s at 30 hertz ...
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bioRxiv - Genetics 2022Quote: ... The tissue powder was then transferred to a 1.5 mL centrifuge tube where 400 μL of 1% PVP-40 Buffer AP1 solution and 4 μL of RNase A were added (Qiagen DNeasy Plant Kit Qiagen Inc, Hilden, Germany). The tube was initially vortexed to homogenize the solution before incubation at 65 °C for 30 minutes with a short vortex every 5 minutes.
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Microbiology 2021Quote: ... approximately 1×106 Jurkat cells were washed twice in PBS and total DNA was extracted with the Qiamp® DNA Minikit (Qiagen, Manchester, UK). Quantitative PCR reactions were carried out as previously described using 0.3pmol each primer and 0.15 pmol of the probe in 25 μL volume containing 100-300ng total DNA using an ABI Prism® 7000 Sequence Detection System (SDS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 1 μg of total RNA was used for cDNA conversion using the RT2 first strand kit (Cat# 330404, Qiagen, Valencia, CA, USA). The prepared cDNA was diluted and used to determine the expression of genes of interest using specific primers ...
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bioRxiv - Biochemistry 2021Quote: ... Medium was stirred for 1 hour at 4°C in the presence of 5 ml Ni-NTA Agarose beads (Qiagen, Venlo, The Netherlands) washed with 25 mM Hepes-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA from untreated or RSL3-treated (with or without 2 hr pre-treatment with 2 µM Ferrostatin-1) MM1S and MM1R cells was extracted using the RNeasy Mini Kit (Qiagen, Venlo, the Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from GFP-empty vector-transfected and GFP-Δ(1-240) JPh1-transfected C2C12 myoblasts using the RNeasy plus mini kit (Qiagen, Hilden, Germany), based on manufacturer instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cancer Biology 2023Quote: Cells were harvested (1×106) by centrifugation (200 x g for 5 min at 25°C. Whole RNA was isolated using the Qiagen RNeasy kit (Qiagen) according to the manufacturer’s description ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time quantitative PCR was performed on 40 pg cDNA using specific primers (Supplementary Table 1) and miRCURY® LNA® miRNA SYBR® Green PCR (Qiagen). Values were normalized to miRNA quantity and expressed as relative expression to control WS using formulae 2−ΔCT.
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were washed once with 1×PBS after which DNA was isolated using the QIAamp DNA Mini Kit (Qiagen, Venlo, The Netherlands) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We verified the PCR products by 1% agarose electrophoresis with EtBr staining and purified using the QiaQuick PCR Purification Kit (Qiagen, Hilden, Germany, 28104) following manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: Genomic DNA (gDNA) was extracted from 1-month-old Arabidopsis thaliana rosette leaves with the DNeasy plant mini kit (Qiagen, cat. no. 69104) per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and 1:10 dilution of cDNA was used to quantify genes using SYBR Green I (QuantiFast® SYBR® Green PCR Kit, Qiagen, USA). ADP-Ribosylation Factor 1 (AB050957.1 ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were weighted using an analytical scale and homogenized in 1 ml of deionized water using TissueRuptor II electric homogenizer by Qiagen (Germantown, MA, USA). RO5256390 was extracted using acetonitrile/water 50%/50% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... The DNA was separated on a 1% agarose gel and the appropriate band was excised and isolated using a gel extraction kit (Qiagen, Valencia, CA, USA). In-Fusion HD enzyme premix was added to the linearized PCR product and transformed into the Stellar™ Competent Cells (Takara ...
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bioRxiv - Genomics 2024Quote: We extracted gDNA from 27 to 100 single-sex worms per pool (Table 1) with the DNeasy Blood & Tissue Kit (Qiagen, Germantown, MD, USA), following the manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Differentially expressed genes (FDR ≤ 0.05, |log2FC|≥ 1) were subsequently filtered considering both gene ontology and IPA annotation (Qiagen Ingenuity Pathway Analysis, v01-13) in order to define receptome and secretome genes lists ...
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bioRxiv - Genomics 2021Quote: ... These leftover materials were collected by LCM and placed into 350 μl of RLT lysis buffer (RNeasy, Qiagen, including 1%β-Mercaptoethanol (β-Me)) followed by RNA extraction and examination with the 2100 Bioanalyzer.
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bioRxiv - Plant Biology 2020Quote: ... Frozen cells were disrupted by bead beating at 30 h/z for 1 min before purifying total RNA using an RNeasy® plant mini kit (Qiagen™; ref. 74904) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 20–30 mg of tissue was taken and placed into a 2 mL tube with 600 µL of RLT buffer with 1% β–mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Hilden, Germany: #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Physiology 2020Quote: ... Samples were subjected to dry pulverization before being homogenized in buffer (350 μL of Qiagen RLT buffer with 1% β-mercaptoethanol; Qiagen, Germantown, MD, USA). The homogenate was transferred to an RNase-free 1.5 mL centrifuge tube ...
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bioRxiv - Pathology 2021Quote: ... 20 – 30 mg of tissue were placed into a 2 ml tube with 600 µl of RLT buffer with 1% β-mercaptoethanol and a 5 mm stainless steel bead (Qiagen, Valencia, CA; #69989). Tissues were then dissociated using a Qiagen TissueLyser II (Qiagen ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from MLEC and lung tissue by lysis in RLT buffer containing 1% β-mercaptoethanol for RNA isolation and subsequently purified using the RNeasy Mini Kit (Qiagen, 74106, Hilden, Germany) according to manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... we ran the digest products on 1% Tris-acetate EDTA gels and extracted and purified the correctly sized products (Qiagen QIAQuick Gel Extraction Kit). In parallel ...