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Citations for Qiagen :
101 - 150 of 159 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: ... The products of restriction enzyme digests were extracted and purified by preparative gel electrophoresis followed by QIAquick gel extraction kit (Qiagen). Restriction endonucleases ...
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bioRxiv - Biochemistry 2021Quote: ... The CYP168A1 optimized cDNA insert was removed from the plasmid using NdeI and HindIII restriction enzymes and isolated using agarose gel electrophoresis and the Qiagen Gel Extraction Kit (Qiagen) for ligation into a similarly digested and isolated pCWOri+ CYP expression vector (113) ...
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bioRxiv - Genetics 2022Quote: ... Reactions were purified using agarose gel electrophoresis and target fragments were excised and purified using QIAquick and MinElute Gel Extraction Kits (Qiagen) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... The restriction digestion efficiency was validated using 1% 1× TBE agarose gel electrophoresis and purified with a QIAquick PCR Purification Kit (Qiagen).
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bioRxiv - Genetics 2020Quote: U8 DNA templates containing a T7 consensus sequence were PCR amplified from human or zebrafish genomic DNA (see Supplementary Table 2 for primer sequences) and purified after agarose gel electrophoresis using a QIAEX II kit (Qiagen). Human and zebrafish U8 snoRNAs were generated using 400-1000ng of template DNA and a mMESSAGE mMACHINE T7 kit (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... Correct and unique amplification of the target regions was verified by agarose gel electrophoresis before purifying PCR products using the QIAquick PCR Purification Kit (Qiagen). For analysis by TIDE ...
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bioRxiv - Microbiology 2020Quote: ... The amplified PCR products were confirmed by agarose gel electrophoresis and purified from the agarose gel using the QIAquick gel extraction kit (QIAGEN) and the Expin™ Gel SV kit (GeneAll ...
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bioRxiv - Immunology 2020Quote: ... The resulting ~440 bp long PCR product was subjected to electrophoresis using an agarose gel and extracted using Gel Extraction Kit (Qiagen).
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bioRxiv - Immunology 2020Quote: ... The resulting ~500 bp long PCR product was subjected to electrophoresis in agarose gels and purified using Gel Extraction Kit (Qiagen). The samples were mixed in equimolar ratios to obtain 30 μl of a final concentration of 10-nM and sequenced by Illumina MiSeq (v3 kit ...
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bioRxiv - Genetics 2019Quote: ... A 5 ul sample was analyzed by electrophoresis on 2% agarose gel and appropriately-digested samples were first purified by PCR purification kit (Qiagen), eluted with 50 ul of 1x NEB Buffer 3 and treated with 10 units of CIP (NEB ...
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bioRxiv - Neuroscience 2022Quote: ... The heavy-chain specific amplicon was isolated using electrophoresis with low-melting point agarose extraction with the QIAquick Gel Extraction kit (Qiagen). A secondary amplification was performed using a modification of the primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was confirmed for correct size by agarose gel electrophoresis and purified using a PCR purification kit (Qiagen). The vector pBOMBL12CRia(topA)::L2 was digested with Fast-digest SalI and treated with alkaline phosphatase as described above ...
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bioRxiv - Microbiology 2022Quote: ... The expected size of PCR products was confirmed by agarose gel electrophoresis and PCR products were purified using QIAquick PCR purification kit (Qiagen). Purified PCR products were sequentially digested with the appropriate restriction endonuclease (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Amplicons were resolved on 1% agarose gel by gel electrophoresis and purified using the QiaQuick Gel Extraction Kit (QIAGEN #28706). Purified PCR product was cloned into the TOPO TA Vector and transformed into OneShot TopTen Chemically Competent E ...
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bioRxiv - Microbiology 2023Quote: Amplified products were assessed for quality using 2% w/v agarose gel electrophoresis in 1X Tris/Borate/EDTA (TBE) buffer and purified using a QIAquick PCR purification kit (Qiagen). The purified libraries were pooled equimolarly and subsequently purified using 0.5X Agencourt AMPure XP beads (A63882 ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run on an agarose electrophoresis gel and the strongest bands excised for purification using a QIAquick Gel Extraction Kit (Qiagen). The purified PCR products were sequenced directly using Sanger sequencing by Genewiz ...
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bioRxiv - Bioengineering 2023Quote: ... the amplified VH and Vκ/Vλ genes were subjected to electrophoresis on a 1.5% agarose gel and purified using a QIAquick gel extraction kit (QIAGEN Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were subjected to electrophoresis on a 1.5% agarose gel and purified using a QIAquick gel extraction kit (QIAGEN Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... the amplified genes were subjected to electrophoresis on a 1.5% agarose gel and purified using a QIAquick gel extraction kit (QIAGEN Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... All reactions from each screen were pooled and the amplified PCR product (~240–250 bp) was purified by agarose gel electrophoresis using the QIAquick gel extraction kit (Qiagen). Purified PCR products were quantified by fluorescence using the Qubit dsDNA high sensitivity assay kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... The amplified library (88 bp) was purified by agarose gel electrophoresis and extracted using the QIAquick gel extraction kit (Qiagen). Purified PCR products were digested with BlpI and BstXI and the digested fragment was purified on a 20% polyacrylamide Tris–borate–EDTA (TBE ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were resolved by agarose gel electrophoresis and JH4 intron sequences with size of 1.2 kb were extracted by QIAquick Gel Extraction Kit (Qiagen; 28706X4). DNA of JH4 sequences was ligated into the pCR-Blunt II-TOPO vector (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... and RNAI were amplified by PCR (primer sequences are listed Supplementary Table 8) and PCR products were analysed by 2 % agarose gel electrophoresis and purified using the QIAquick PCR purification kit (QIAGEN). 5’-triphosphate (PPP ...
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bioRxiv - Genomics 2023Quote: ... Next the correct sizes of the linearized backbones were isolated using gel electrophoresis and later purified using QIAquick Gel Extraction Kit (QIAGEN). For the library of 7 domains ...
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bioRxiv - Molecular Biology 2023Quote: Size of PCR fragments was verified using gel electrophoresis and DNA was purified using the QIAquick PCR Purification Kit (Cat# 28104, Qiagen). Concentration was measured using a Quantus™ Fluorometer (Promega ...
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bioRxiv - Genomics 2023Quote: ... The amplicon library pools were isolated based on size by gel electrophoresis using a 2% agarose gel and then purified using QIAEX II Gel Extraction Kit (QIAGEN) and using 30uL of QIAEX II beads for each sample ...
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA fragment was separated by electrophoresis using a 1% agarose gel and purified using QIAquick Gel Extraction Kit (QIAGEN). The genome sequence of drp-1 was determined by Sanger sequencing using primers listed in Supplementary Table S2.
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bioRxiv - Genetics 2024Quote: ... and proteinase K was inactivated at 95°C for five minutes.39 PCR was performed on isolated genomic DNA followed by analysis on a QIAxcel Advanced System instrument using capillary electrophoresis (Qiagen). Primer3plus45 was used to design primers and oligonucleotides obtained from IDT (Supplementary Table 1).
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bioRxiv - Synthetic Biology 2021Quote: ... PCR products were analysed by gel electrophoresis using 1% agarose gel and purified using QIAquick gel extraction kit (Qiagen, Hilden, Germany). The DNA concentrations of the gel-purified samples were quantified with Nanodrop OneC (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... between 264 bp and 464 bp were re-separated and purified from the gel band in electrophoresis by QIA quick gel extraction kit (Qiagen, Hilden Qiagen, Germany); and the paired obtained sequences (terminal 125 bp ...
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bioRxiv - Zoology 2020Quote: 5µL of each PCR product were size-fractioned by gel electrophoresis and the remaining 45µL of the PCR product were cleaned up with a QIAquick PCR Purification Kit (QIAgen, Hilden, Germany) if size estimation and expected sequence length matched ...
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bioRxiv - Neuroscience 2019Quote: ... a 92kb Egr1-FRT-ERT2-Cre-ERT2-FRT BAC fragment generated from PmeI and PacI double digestion and a 61kb Snap25-Flpe BAC fragment generated from SbfI and PmeI double digestion was purified using pulsed field gel electrophoresis followed by gel extraction (Qiagen #20021). The pronuclei of fertilized one-cell eggs of C57BL/6j mice were co-injected with a mixture of equimolar amounts of the two fragments by the Department of Pathology Knockout ...
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bioRxiv - Bioengineering 2021Quote: ... The 319 bp DNA size of PCR products were clarified by 1% agarose gel electrophoresis using 5 μL PCR products and remained DNA were purified by QIAquick PCR Purification Kit (Qiagen, 28104). The purified PCR products were sequenced by Sanger Sequencing approach (GeneWiz ...
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bioRxiv - Plant Biology 2021Quote: ... After agarose gel electrophoresis the PCR products were extracted from the gel with the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) and inserted into a pENTR vector (pENTR™/D-TOPO™ Cloning Kit ...
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bioRxiv - Genetics 2019Quote: ... Insertion and deletion mutations were detected by PCR and resolved by agarose gel electrophoresis or using a Qiaxcel (Qiagen, Hilden, Germany). Primers were designed using Primer343 and oligonucleotides ordered from Integrated DNA Technologies (Table 1).
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bioRxiv - Microbiology 2020Quote: ... and amplicon length was measured by comparison against QX DNA Size Marker using the Qiaxcel Automatic electrophoresis system (QIAGEN, Hilden, Germany). Samples were sequenced by the Center for Translational Genomics and BioInformatics (Milan ...
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bioRxiv - Biochemistry 2022Quote: ... All PCR products and digested plasmid were separated by agarose gel electrophoresis and extracted from the gel with QIAquick® Gel Extraction Kit (Qiagen). After assembly ...
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bioRxiv - Biochemistry 2022Quote: ... All PCR products and digested plasmid were separated by agarose gel electrophoresis and extracted from the gel with QIAquick® Gel Extraction Kit (Qiagen). After assembly ...
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bioRxiv - Microbiology 2022Quote: ... PCR products of approximately 440 bp were visualised by gel electrophoresis and gel-purified using the QIAEX II Gel Extraction Kit® (QIAGEN). The purified barcoded amplicons were quantified by Qubit™ dsDNA BR Assay Kit (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... amplicons were subjected to agarose gel electrophoresis for product size selection and purified with QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Ligation was performed as previously described [10] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we performed agarose gel electrophoresis and excised DNA fragments of 200 to 400 bp that were purified using MinElute columns (Qiagen, 28604). For DMC1-SSDS ...
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bioRxiv - Microbiology 2019Quote: ... gel electrophoresis of the remaining amplicon was followed by purification of the excised band using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Dye terminator sequencing was performed on the CEQ8000 Genetic Analysis System (SCIEX ...
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bioRxiv - Genetics 2021Quote: ... and 200-500 bp fragments were extracted from a 2% agarose gel after electrophoresis and purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany). The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Target bands were cut from electrophoresis gels and the PCR products were extracted using a QIAquick gel extraction kit (QIAGEN, Hilden, Germany). Extracted products were amplified by PCR using a forward primer (Primer for sequencing ...
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bioRxiv - Microbiology 2022Quote: ... triplicate reactions were re-pooled using the epMotion and DNA concentrations were quantified using a QIAxcel Advanced capillary electrophoresis system (Qiagen, Hilden, Germany). Samples with concentrations > 0.5 ng/µl were pooled using equal DNA masses to create the final sequencing libraries ...
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bioRxiv - Microbiology 2021Quote: ... triplicate reactions were re-pooled using the epMotion and DNA concentrations were quantified using a QIAxcel Advanced capillary electrophoresis system (QIAgen, Hilden, Germany). Samples that had concentrations > 0.5 ng/μl were pooled using equal weights of DNA to create the final sequencing libraries ...
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bioRxiv - Microbiology 2019Quote: ... gel electrophoresis of the remaining amplicon was followed by purification of the excised band using QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). Dye terminator sequencing was performed on the CEQ8000 Genetic Analysis System (SCIEX ...
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bioRxiv - Microbiology 2020Quote: ... PCR product sizes and quality were controlled by running gel electrophoresis and DNA fragments were purified using a QIAquick PCR Purification Kit (Qiagen, Hilden, Germany).
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bioRxiv - Genetics 2021Quote: ... in length were extracted from a 2% agarose gel after electrophoresis and purified using a Qiagen Gel Extraction Kit (Qiagen, Hilden, Germany). The purified DNA was PCR amplified using GoTaq Colorless Master Mix (Promega ...
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bioRxiv - Biophysics 2021Quote: ... they were concentrated and purified using gel electrophoresis and separated from the gel using the Gel Extraction Kit from Qiagen (Hilden, DE). DNA concentration was then determined using NanoDrop Spectrophotometer (ND-1000 ...