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Citations for Qiagen :
101 - 150 of 6275 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... PNGase F and the cleaved 10 × His tag were removed by passing the sample through Ni-NTA superflow resin (QIAGEN). The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore) ...
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bioRxiv - Biophysics 2021Quote: ... NF-κB molecules were immobilized through the 6xHis-tag on the C-terminus of RelA by penta-His antibody biotin conjugate (34440, Qiagen). For DNA-bound NF-κB experiments ...
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bioRxiv - Biophysics 2022Quote: ... Cell debris was cleared by centrifugation at 40,000 g for 45 min at 4°C and supernatant was applied to His-tag affinity purification using Ni-NTA agarose (beads or 5 ml column, Qiagen) in buffer A (50 mM TRIS ...
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bioRxiv - Microbiology 2023Quote: ... Pulled-down fractions were analysed by SDS-PAGE or Western blot using rabbit antibodies against KtrB or mouse against His-tag (Qiagen).
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bioRxiv - Neuroscience 2021Quote: ... and transiently transfected in S2 cells using Effectene (QIAGEN, catalog no. 301425). Cells were collected three days post-transfection ...
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bioRxiv - Cell Biology 2020Quote: Drosophila adult flies or Drosophila S2 cells were homogenised using QIAshredder (QIAGEN) and total RNA was extracted with RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... S2 cells were transfected with indicated constructs using Effectene transfection reagent (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... synthetic RNA spike-ins (UniSp2, UniSp4, UniSp5 RNA Spike-in mix; Qiagen) were added and used as quality controls for RNA isolation ...
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bioRxiv - Microbiology 2019Quote: ... Short CAP was expressed and His tag purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen, Hilden, Germany). The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatants containing TtgR and SmtB were purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The eluted fractions from the FPLC (for TetR ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Genomics 2019Quote: ... Insects were immediately dissected in RNAlater (Qiagen) to stabilize expression profiles and kept deeply frozen until RNA extractions ...
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bioRxiv - Biochemistry 2021Quote: ... S2 cells were transfected with pA-EB1-GFP using Effectene Transfection Reagent (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... pMT-pHluorin2-mCherry was transfected in S2 cells using Effectene transfection reagent (Qiagen) with a 1:10 ratio of DNA to Effectene Reagent.
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bioRxiv - Microbiology 2021Quote: ... into S2 cell lines using Attractene Transfection reagent following the manufacturer’s protocol (Qiagen). A “no miRNA” treatment with only psiCheck-2 plasmid and transfection reagent was also conducted ...
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bioRxiv - Developmental Biology 2021Quote: S2 cells were transfected with Btz-WT or Btz-HD using Effectene (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... The RBD wildtype and mutant proteins of SARS-CoV-2 spike with 10×his tag at N terminal were expressed in HEK 293 and purified with Ni-NTA affinity columns (Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed at indicated time points after warming and then processed for immuno-identification of HPK using an antibody that recognizes the polyhistidine tag (RGS-His antibody; Qiagen 1:100) and counterstained for nuclei using DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... His-tag fusions were purified by incubating the bacterial soluble fraction with pre-equilibrated Ni-NTA agarose bead slurry (Qiagen, Hilden, Germany) for 1 hour at 4°C and eluting with 500 mM imidazole ...
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bioRxiv - Immunology 2021Quote: ... and HRP-anti-His (Penta-His Ab, Qiagen) was used in the detection step ...
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bioRxiv - Molecular Biology 2023Quote: ... Insect cells were transfected with SMS/pMT/V5-HisB constructs using Effectene (Qiagen, Hilden, Germany) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) that spanned a wide range of concentrations were added to the recovered RNAs according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... A set of 52 RNA spike-ins (QIAseq miRNA Library QC Spike-Ins – Qiagen) spanning a wide range of molar concentrations were added to the recovered RNAs ...
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bioRxiv - Developmental Biology 2021Quote: ... S2 cells were pelleted 48 hours after transient transfection using Effectene Transfection Reagent (Qiagen) and washed in fresh medium ...
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bioRxiv - Genomics 2022Quote: Total RNA from S2 cells was isolated using the RNeasy Plus Mini Kit (Qiagen). Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Plasmids of 5 µg were transfected into 6x10[6] S2 cells using Effectene (Qiagen) and incubated for 3 days ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from S2 cells using the RNeasy Plus Mini Kit (Qiagen 74134). RNA was reverse transcribed using the Maxima H minus system (Thermo Fisher K1681).
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bioRxiv - Evolutionary Biology 2020Quote: ... The pAC5.1-miRNA (300 ng) was transfected into Drosophila S2 cells using Effectene (Qiagen) per the manufactures’ instructions ...
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bioRxiv - Genomics 2021Quote: ... Transient Transfections of S2 cells were performed using Qiagen Effectene Transfection reagent (Qiagen 30425) at a 1:25 DNA-Enhancer ratio ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted from S2 cells using the RNeasy Plus Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then stained by Coomassie-blue or immunodetected as described before [26] using primary polyclonal antibodies directed against His6 epitope-tag (Penta His, Qiagen, dilution 1:1000), V5 epitope-tag (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: Proteins were purified from Sf21 insect cells by affinity purification with Ni-NTA agarose (Qiagen, 30230) and ...
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bioRxiv - Genomics 2020Quote: ... S2 cells were transfected with 1 ug plasmid DNA using the Effectene reagent kit (Qiagen). The plasmid DNA which contains cDNA of FLAG-tagged ECDs are under the metallothionein promoter control ...
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bioRxiv - Molecular Biology 2020Quote: Drosophila S2 cells were transfected with 3μg of pAWM-dfr4 using Effectene transfection kit (Qiagen) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... S2 cells were transiently transfected with pAc-brm-FLAG-6xHIS using Effectene (Qiagen, Valencia, CA). Cells were harvested 48 hours after transfection and proteins were extracted using EB2 buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: S2 cell transfections were carried out in 6-well plates using Effectene transfection reagent (Qiagen). Each well was transfected with 1 μg of the pAc5.1-λN-HA:dFMRP and 1 μg pAFW-AGO1 or pAFW-GW182 plasmids ...
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bioRxiv - Molecular Biology 2023Quote: S2 cell transfections were carried out in 6-well plates using Effectene transfection reagent (Qiagen). Each well was transfected with 1 μg of the pAc5.1-EGFP:dFMRP and 1 μg of pAc5.1-mCherry plasmids containing DCR1 ...
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bioRxiv - Systems Biology 2023Quote: ... 1ul ExiSEQ NGS Spike-in (Qiagen) was added to the extracted RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... penta-HIS (QIAGEN) or α-RF1 E.coli (Zaher Lab ...
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bioRxiv - Cell Biology 2021Quote: ... penta-His (Qiagen). Membranes were then incubated with fluorophore-conjugated secondary antibodies diluted 1:20,000 in TBS-T and 5% BSA ...
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bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
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bioRxiv - Biochemistry 2022Quote: ... the lysate was supplemented with Ni-NTA agarose (Qiagen, 1.3 ml resin/ 1 l insect cell pellet). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
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bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting pMT-ball-FLAG vector was transfected into S2 cells using Effectene transfection reagent (Qiagen) and stable cell line was generated by following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Those included UniSpike 2 and 4 from the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN, Hilden, Germany), which were used to monitor RNA isolation efficacy ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...