Labshake search
Citations for Qiagen :
101 - 150 of 737 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
-
bioRxiv - Microbiology 2024Quote: ... GnTi expressed His-tagged gp140s and gp120s were purified from the harvested and clarified supernatant using Ni-NTA agarose beads (Qiagen) following manufacturer’s instructions.
-
bioRxiv - Microbiology 2024Quote: To determine bispecificity of bsAbs his-tagged HCV E2 (3 μg/mL in Tris-buffered saline (TBS)) was immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... Culture supernatant containing His-tagged NTD protein was harvested 4 days after transfection and was purified using Ni-NTA resin (Qiagen). Spike protein was further purified with a Superose 6 Increase 10/300 GL column equilibrated with 20 mM Tris-HCl (pH 8.0) ...
-
Biosynthetic characterization and combinatorial biocatalysis of the cysteine protease inhibitor E-64bioRxiv - Biochemistry 2024Quote: ... The recombinant hexa-His-tagged proteins were purified individually from corresponding soluble fractions by affinity chromatography with Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: Human CRM1 was expressed as His-tag fusion protein from a pQE60 plasmid (Qiagen) in E ...
-
bioRxiv - Plant Biology 2020Quote: ... His-tagged CLEL6 precursor was purified from bacterial extracts by metal chelate affinity chromatography on Ni-NTA Agarose (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
-
bioRxiv - Plant Biology 2020Quote: ... His-tagged PSK1 or RGF1 precursors were purified from bacterial extracts by metal chelate affinity chromatography on NiNTA Agarose (Qiagen, Hilden, Germany) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2021Quote: ... coli BL21 (DE3) and expression of the His-tagged Cas proteins was performed following the instruction of the protein purification kit (Qiagen, Valencia, CA, USA). Single colonies of transformed cells were cultivated overnight ...
-
bioRxiv - Immunology 2021Quote: ... and HRP-anti-His (Penta-His Ab, Qiagen) was used in the detection step ...
-
bioRxiv - Plant Biology 2024Quote: ... His-tag (Penta-His HRP Conjugate, ID 34460, Qiagen), Actin (A0480 ...
-
bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
-
bioRxiv - Cell Biology 2021Quote: ... penta-His (Qiagen). Membranes were then incubated with fluorophore-conjugated secondary antibodies diluted 1:20,000 in TBS-T and 5% BSA ...
-
bioRxiv - Microbiology 2022Quote: ... α-His antibody (Qiagen) was used at 1:5,000 dilution to detect the presence of rRH5 in Native-PAGE.
-
bioRxiv - Biophysics 2020Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used.
-
bioRxiv - Biochemistry 2022Quote: ... anti-His antibodies from the Penta-His HRP Conjugate Kit (Qiagen) were used ...
-
bioRxiv - Plant Biology 2022Quote: ... bound His-mSTIC2 protein was detected by anti His HRP conjugate (Qiagen) and ECL reaction (Pierce).
-
bioRxiv - Cell Biology 2020Quote: ... Penta-His from QIAGEN; CD63 (clone H5C6 ...
-
bioRxiv - Microbiology 2020Quote: ... His-Penta-Conjugate (Qiagen) was then used as the secondary antibody (1:5000) ...
-
bioRxiv - Microbiology 2024Quote: ... anti-penta-His (Qiagen), anti-PNPase (generated by Scrum ...
-
bioRxiv - Immunology 2020Quote: ... and detection was performed using anti-His antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse antibody labeled with AlexaFluor647 (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... His-EHD1 was purified by binding with Ni2+-NTA His-bind slurry (Qiagen, Germany) and eluting with imidazole as described previously (65) ...
-
bioRxiv - Biophysics 2021Quote: ... 2 g/ml Biotinylated Anti-His Antibody (Penta-His Biotin Conjugate; Qiagen; No. 34440) in T50 buffer was introduced into flow cells at 50 μl/min ...
-
bioRxiv - Cell Biology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... and detection was performed using anti-His primary antibody (Penta-His Antibody, #34660, Qiagen) followed by donkey-anti-mouse secondary antibody labeled with AlexaFluor647 (Invitrogen ...
-
bioRxiv - Biochemistry 2020Quote: ... anti-RGS-His (Qiagen, 34610), anti-Vinculin (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-penta-His (34660, Qiagen), anti-GFP-HRP (B-2 ...
-
bioRxiv - Biochemistry 2021Quote: ... and anti-His (Qiagen 34660) antibodies ...
-
bioRxiv - Biophysics 2024Quote: ... membranes were incubated under soft shaking with Penta-His antibody (Penta His HRP Conjugate; Qiagen) for 1 h with 0.5 % (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... His-NusA-FASP or His-NusA-Degron fusion proteins were purified using Ni-NTA resin (Qiagen) using standard approaches and His-TEV protease was added to cleave the His-NusA tag from the PER2 peptides on column at 4°C overnight ...
-
bioRxiv - Cancer Biology 2021Quote: ... HIS-tag (Qiagen 34610 1:100) and HER3 (R&D Systems AF4518 1:400) ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse anti-His (Qiagen, number 34660); Mouse anti-β-actin (Proteintech ...
-
bioRxiv - Biochemistry 2022Quote: ... A mouse monoclonal His-tetra (Qiagen) antibody (1:1000 dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... Anti-His Western blots were performed using Penta-His mouse monoclonal IgG1 as primary antibody (Qiagen, #34660) at 1:2000 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... Cleaved CK1δ ΔC was further purified away from His-GST and His-TEV using Ni-NTA resin (Qiagen) and subsequent size exclusion chromatography on a HiLoad 16/600 Superdex 75 prep grade column (GE Healthcare ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-His tag (1:2000; QIAGEN) and rabbit anti-GST (1:2000 ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse anti-His from Qiagen (1:10,000), home-made rabbit anti-VASH1 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... primary mouse anti-His antibody (QIAGEN, 34660), secondary goat anti-rabbit 800CW antibody (LI-COR ...
-
Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... the Penta-His HRP Conjugate Kit (Qiagen) was used following manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... the mouse monoclonal Penta-His antibody (Qiagen n°34660 stock at 200 µg/ml ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-RGS-His (Qiagen, 1:2000, 34610), anti-Myc (ChromoTek ...
-
bioRxiv - Biochemistry 2022Quote: ... Mouse anti-His (Qiagen, 34670; 1:200) and goat anti-mouse IgG-AF488 (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... The Penta-His HRP Conjugate Kit (Qiagen) was used for detection of His-tagged proteins according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and anti-His mAb (QIAGEN, Hilden, Germany) was added (1 µg/mL in PBS supplemented with 1% w/v BSA) ...
-
bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with mouse primary antibody α-His (1:5000, Monoclonal mouse Tetra-His antibody, Qiagen, #34670) in 2.5% BSA in PBS-T overnight at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... DNA was extracted from plant cells and Hi-C libraries were prepared using EpiTect Hi-C Kit (Qiagen, Germany) according to the manufacturer’s protocol ...
-
Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... LCAT expression in 293T cells was verified by western blot with an anti-His antibody (Qiagen Penta-His Antibody). LCAT was purified by Ni Sepharose excel (Cytiva ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing His-eIF4A1 or His-eIF4A2 was incubated with a 1.5 ml bed volume of Ni-NTA Superflow (Qiagen, 30430) or a 0.75 ml bed volume of Ni-NTA agarose (Qiagen ...