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Citations for Qiagen :
101 - 150 of 637 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Purification of C-terminal His6-tag-labeled GluN1 and GluN1ΔM4 by Ni2+-NTA agarose (Qiagen) chromatography was performed as in (Madry et al. ...
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bioRxiv - Cell Biology 2020Quote: ... the supernatant was incubated for 1 hour at 4°C with Ni-NTA-agarose (Qiagen), washed several times with lysis buffer and the protein was eluted by incubation for 3 hours with lysis buffer containing 500 mM imidazol ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Neuroscience 2023Quote: ... The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen, 36111), which were subsequently collected ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by enzyme inactivation 20 minutes at 65°C and column-based purification (Qiagen, #74204). First strand cDNA synthesis was performed with the High-Capacity-RNA-to-cDNA Kit (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... Sorted Ly6G+ cells were stored at −80°C in RLT Plus Buffer (Cat# 1053393-Qiagen). Total RNA isolation was done using RNeasy Plus Mini Kit (Cat# 74134-Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, 28004) to produce tagmented DNA samples ...
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bioRxiv - Microbiology 2024Quote: ... steps were carried out at 4°C centrifuge as described previously using RNeasy kit (Qiagen) [84] ...
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bioRxiv - Microbiology 2024Quote: ... and stored at -20°C until extraction using the DNeasy Blood and Tissue kit (Qiagen). qPCR assays for polA and rabbit β-actin were performed as previously described (40 ...
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bioRxiv - Cancer Biology 2024Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, #28004) to produce tagmented DNA samples ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated for 2h at 56°C in ATL buffer and Proteinase K (Qiagen) to allow complete lyse ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Light organs were then transferred into 180 ul of pre-heated (98°C) ATL buffer (QIAGEN) and incubated at 98°C for 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated for 45min at 42°C and the reaction purified with 15µl of MagAttract beads (Qiagen). The labelled DNA was eluted with TE-buffer (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2020Quote: An RNA probe targeting c-myb was generated using one-step RT-PCR (Qiagen, Manchester, UK) using the following primers c=myb-F 5’-CCAAGTCAGGAAAACGCCACCTCG-3’ and c-myb-R 5’-GCTGTTGTTTAGCGGAGTTGGGCT-3’ and cloned into the dual promoter vector pCRII-TOPO (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... then spun at 14000K RPM for 10 minutes at 4°C to pellet debris (Qiagen, 37901). Protein concentration in the supernatant was quantified using BCA assay (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and total RNA was extracted from the supernatant using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... for 20 min at 37°C followed by purification with the QIAquick PCR purification kit (Qiagen). To quantify the RNA transcripts ...
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bioRxiv - Biophysics 2020Quote: ... Enzyme was inactivated (65°C, 15min) prior to PCR purification of the linearized product (Qiagen, Germany). 10μg of linearized plasmid was transformed into Pichia pastoris X-33 strain by electroporation as per the manufacturers protocol (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) and supernatant was transferred to tubes containing 30µl precleared Ni-NTA agarose beads (Qiagen) in the presence of 0.05% Tween-20 and 15mM imidazole ...
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bioRxiv - Genomics 2021Quote: Skeletal muscle previously stored at −80°C was lysed with the RLT buffer Plus buffer (Qiagen) and beta-mercaptoethanol using the TissueLyser II (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... for 20 min at 37°C and purification with the RNeasy MinElute Cleanup kit (Qiagen, #74204). RNA quality was checked with the Agilent 2100 Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: ... snap frozen (−80°C) for proteomics analysis or dissolved in 300 ul of RNAlater reagent (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... at 37°C for 30 min and further purified using an RNeasy Mini Kit (Qiagen, 74104) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 1 h at 55°C with 20 mg ml−1 proteinase K (Qiagen). Samples were treated for 10 min at 65 °C with 4 μl RNase A (100 mg ml−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Hypothalamic blocks were dissected and stored at − 80°C until RNA extraction using RNAeasy kits (Qiagen).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were stored at -80°C with the addition of RNA protect reagent (Qiagen, MD, USA). DNA was extracted using DNeasy PowerSoil kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN) (1 h at 50°C and then overnight at 37°C) ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was eluted two times with 50 μl of pre-heated (70°C) EB (Qiagen, 80204) through centrifugation at 8,500×g for 1 min as documented previously10 ...
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bioRxiv - Molecular Biology 2022Quote: ... Further these lysates were incubated overnight at 4°C with prewashed Ni-NTA beads (Qiagen, 30210).Sequential washes with wash buffer 1-4 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... 3 min in 37°C shaker) and processed with DNeasy Blood and Tissue Kit (QIAGEN #69504). Two hundred ng of genomic DNA was used as input in the DamID protocol that included an improved pool of AdR primers as described (de la Cruz Ruiz et al ...
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bioRxiv - Evolutionary Biology 2022Quote: ... tissues were incubated overnight at 56 °C in 300 μL of ATL digestion buffer (Qiagen®) including proteinase K (Cf = 1 mg/ mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were incubated at 37°C for 15 minutes following addition of 2µg RNase A (Qiagen). 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were washed once with PBS (37 °C) and 350 µl RNA lysis buffer (Qiagen, Buffer RLT
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bioRxiv - Molecular Biology 2023Quote: ... at 37°C for 30 min followed by clean-up using an RNeasy Mini kit (Qiagen) and the modified transcripts of SINEUP-GFP RNA were recovered.
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bioRxiv - Plant Biology 2024Quote: ... 65°C treatment with EDTA and cleaned with the RNeasy MinElute Cleanup Kit (Qiagen, Hilden, Germany). Depletion of ribosomal RNA (rRNA ...
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bioRxiv - Neuroscience 2023Quote: ... Colonies were cultured for 16 hr at 37°C and DNA was prepared by maxiprep (Qiagen). The entire homology arm sequence and the whole assembled sequence was verified (Genewiz).
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bioRxiv - Cell Biology 2023Quote: ... for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, Cat. #28004) to produce tagmented DNA samples ...
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bioRxiv - Immunology 2022Quote: ... at 50 °C for 1 hour and were purified with a MinElute PCR Purification Kit (QIAGEN).
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bioRxiv - Evolutionary Biology 2024Quote: ... Extractions were taken from whole blood samples using the Gentra Puregene Blood Core Kit C (QIAGEN), and libraries prepared using the Nextera DNA Flex Library Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... 4°C) and total RNA was extracted from the supernatant using the RNeasy Mini Kit (Qiagen) or the E.Z.N.A Total RNA kit I (Omega-Biotek) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were stored at -20°C until extraction using the DNeasy Blood and Tissue kit (Qiagen). qPCR assays for polA and rabbit β-actin were performed as previously described (40 ...
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bioRxiv - Cancer Biology 2024Quote: ... at 55 °C for 2 hr before recovered using a miniElute PCR purification kit from Qiagen. ChIP-qPCR was performed using primers listed in Supplementary Table 5 and PowerUp SYBR green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was incubated for 2 h at 4°C while rotating with Glutathione superflow beads (Qiagen) for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at 56°C for one hour then were homogenized using tungsten beads and a TissueLyser (Qiagen) for one minute at 30 cycles/sec ...
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bioRxiv - Genomics 2024Quote: ... at -20°C or column clean up using RNeasy MinElute Cleanup Kit (Qiagen, cat. no. 74204).
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bioRxiv - Biochemistry 2024Quote: ... for 4 h at 37 °C and purified with PCR purification kit (QIAGEN, catalog no. 28106) per 20 μL IVT reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were stored at −80°C according to the protocol of RNeasy Micro Kit (QIAGEN).
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bioRxiv - Microbiology 2020Quote: DNA was extracted from -80°C stored stool samples using the DNeasy Powersoil HTP 96 kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Microbiology 2020Quote: ... The soluble fraction was incubated for 1 hour at 4 °C using a Ni-NTA resin (Qiagen). The Ni-NTA resin was washed with 4 times resin volume (RV ...