Labshake search
Citations for Qiagen :
101 - 150 of 205 citations for Adenovirus Type 40 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Evolutionary Biology 2023Quote: ... DNA and RNA were extracted from the B14804-/- and wild-type cells using AllPrep DNA/RNA Mini kit (Qiagen). cDNA was synthesized from extracted RNA using SuperScript™ III Reverse Transcriptase (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... rdr1;2;6 Cen5-ATHILA5 ddm1 (hp5) and their corresponding DDM1 wild-type siblings with the DNeasy Plant Pro Kit (Qiagen). From each sample ...
-
bioRxiv - Microbiology 2023Quote: ... oxydans wild-type cultures at OD600 0.3 units (Exp) or 3 units (Sta) using the RNeasy Mini Kit (Qiagen), following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... the circular DNA (40 ng) was subjected to rolling circle amplification (RCA) using Repli-G midi kit (Qiagen). RCA is a technique used for multiple amplification of circular DNA to obtain sufficient amplicons for next-generation nucleotide sequencing (NGS ...
-
bioRxiv - Plant Biology 2019Quote: ... DNA was extracted from approximately 40 mg of dry leaf material using the DNeasy Plant Mini Kit (Qiagen). Total DNA (0.2-1.0 μg ...
-
bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 40 ng of isolated RNA using the RT2 First strand Kit (Cat#330404 Qiagen). Pre-Amplification of cDNA was done using Single cell to CT kit (Cat#445 8237 Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... 30-40 mg of the sample were incubated in 100 μl of Extraction Buffer EXB Plus (Qiagen, Germany) containing 6% β-mercaptoethanol on ice for 5 min and mixed by vortexing ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was extracted from ∼40 mg of each sample using the DNeasy Plant Mini Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
-
bioRxiv - Cell Biology 2023Quote: ... and 40 ng of cDNA was used for each qPCR reaction with QuantiTect SYBR green reagent (QIAGEN #204143). PCR primers for specific poly(A ...
-
bioRxiv - Microbiology 2024Quote: Cells were grown to 40% confluence and then incubated with 50 nM siRNA and Attractene transfection reagent (QIAGEN) for 36 h according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2021Quote: ... allowing resolution of a 230-bp product for the wild-type allele and a 194-bp product for the mutant allele (DNeasy 96 Blood and Tissue Kit, Qiagen).
-
bioRxiv - Genomics 2022Quote: ... The genomic DNA of the wild-type and the mutant strains was isolated using a QIAamp DNA Mini Kit (QIAGEN). Libraries were prepared using the Nextera XT DNA Library preparation kit (Illumina) ...
-
bioRxiv - Genomics 2020Quote: Thirty-three RNA samples were extracted from three biological replicates of each of 11 diverse tissue types of A188 with RNeasy Plant Mini Kit (Qiagen) (Supplementary Table 10) ...
-
bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
-
bioRxiv - Developmental Biology 2020Quote: ... ∼100 ng of total RNA from each cell type was used as input to generate miRNA sequencing libraries using a QIAseq miRNA library kit (Qiagen). Single-end 100 bp reads (1×100 ...
-
bioRxiv - Molecular Biology 2020Quote: RNA was extracted from 14-day-old wild-type and acinus-2 pinin-1 seedlings using RNeasy mini kit (Qiagen) and treated with TURBO DNA-free Kit (Ambion ...
-
bioRxiv - Immunology 2021Quote: ... cDNA was quantified using 96-well PCR array analysis on a PAMM-150ZA plate (Cytokines & Chemokines) and PAMM-016ZA plate (Type I Interferon Response) (both Qiagen). Quantitative real time-PCR (QRT-PCR ...
-
bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from 5-day-old wild-type and Mpatg mutant thalli using the RNeasy Plant Mini Kit (Qiagen) and used as a template for reverse transcription using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2021Quote: ES2 human clear cell carcinoma cells present (wild type) or absent (siRNA) ARID1A were collected and extracted using an RNeasy Plus Micro Kit (Qiagen). Barcoded TruSeq RNA v2 libraries (Illumina ...
-
bioRxiv - Genetics 2022Quote: ... DNA was extracted from a wild type clone and a mutant using a QIAGEN Plasmid Midi Kit (Qiagen, Tokyo, Japan) and used as standard DNA to determine ratios of mosaicism ...
-
bioRxiv - Cancer Biology 2022Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA)(42) (Qiagen) and Genomic Regions Enrichment of Annotations Tool (GREAT)(43) ...
-
bioRxiv - Developmental Biology 2020Quote: Total RNA was extracted from 20 trunks of either E8.5 wild-type or Aldh1a2-/- embryos with the RNeasy Micro Kit (Qiagen #74004). Reverse transcription was performed with the High-Capacity cDNA RT Kit (Thermo Fisher Scientific #4368814) ...
-
bioRxiv - Genomics 2019Quote: ... and two control bulls that were homozygous for the wild type allele was extracted from testis tissues using the RNeasy Mini Kit (Qiagen) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2019Quote: ... total RNAs were extracted from wild-type and Cables2-deficient EpiLCs at 2 days post-induction (n = 3) using RNeasy Plus Mini Kit (Qiagen). RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan ...
-
bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
-
bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
-
bioRxiv - Microbiology 2021Quote: DNA extraction from cultured tprAko-SS14 or wild-type strains propagated in 6-well plates following the transformation procedure was performed using the QIAamp mini kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: E9.5 hindbrain spanning rhombomeres 1-6 were dissected from wild type and mutant embryos (n=3) to obtain total mRNA preparations (miRNeasy Micro Kit, Qiagen). Sequencing libraries were prepared following the SMART-Seq v4 Ultra Low Input RNA (TaKaRa ...
-
bioRxiv - Genetics 2021Quote: Genomic DNA was extracted from one wild-type control and three patient cell lines using DNeasy Blood and Tissue kit (Qiagen). Specific primers were designed to cover approximately 500 base pairs around the mutations and the regions were PCR-amplified using Qiagen Multiplex PCR kit (Qiagen) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... with tissue homogenization being performed using a rotor stator type tissue homogenizer (ProScientific Bio-Gen PRO200 Homogenizer; Multi-Gen 7XL Generator Probes) in RLT Buffer (Qiagen). RNA quality was assayed using a nanodrop ...
-
bioRxiv - Genetics 2022Quote: Total RNA was isolated from 50 to 100 μl of packed worms from wild type and brd-1(null) using the RNeasy Mini Kit (74104; Qiagen) and QIAshredder (79654 ...
-
bioRxiv - Neuroscience 2022Quote: Brain and spinal cord tissue from Prp-TDP43A315T (Tg) animals and wild type (Wt) littermates were homogenized using a TissueRuptor (Qiagen). 1 mL of TRIzol per 250 mg of tissue was used for homogenization and RNA was isolated as per the manufacturer’s instruction ...
-
bioRxiv - Plant Biology 2023Quote: Two-week-old Arabidopsis seedlings of Col-0 wild type and trb1/2/3 triple mutans were used for DNA extraction using DNeasy Plant Mini Kit (QIAGEN). A total of 500 ng DNA was sheared with Covaris S2 (Covaris ...
-
bioRxiv - Molecular Biology 2023Quote: ... cf-DNA quantified from various plasma or serum types using either MitoQuicLy or a silica-based membrane column DNA extraction kits (DNeasy, Qiagen). A total of 50 samples were analyzed (5 biofluids ...
-
bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from adult ovaries of transgenic or wild-type zebrafish using the RNeasy Mini kit (QIAGEN, 74134) and reverse-transcribed using SuperScript IV reverse transcriptase (ThermoFisher ...
-
bioRxiv - Biochemistry 2023Quote: ... A linearized PCR template was generated by digesting a wild-type Hrd1 plasmid with restriction enzyme and purified using a QIAquick PCR purification kit (Qiagen). For each region two separate PCR reactions were set up ...
-
bioRxiv - Evolutionary Biology 2023Quote: RNA from HeLa cells transfected with either wild-type PADI2 (WT) or mutant PADI2 (with L642A, or L642A/W161A, or L642A/W161A/T159A) was extracted using RNeasy (Qiagen) according to the manufactureŕs instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA) (Qiagen) (90).
-
bioRxiv - Molecular Biology 2024Quote: RNA isolation from leaf tissues of five-week-old non-transformed wild type and overexpressing transgenic lines (two replicates of wild type and two transgenic lines) were isolated as per manufacturer’s instruction (Qiagen, Germany). High quality RNA samples with 200 ng/μL concentration were outsourced for RNA-seq ...
-
bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from two-week-old Villersexel wild type and the nog1dis mutant using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Genomic DNA was isolated from 40 adult flies using a modified protocol for the QIAamp DNA mini kit (QIAGEN) (Simhadri et al. ...
-
bioRxiv - Genetics 2021Quote: ... The 10 μL reaction was carried out with 40 ng genomic DNA and 0.2 μM of each primer and 5 mM Multiplex PCR Kit (Qiagen). Cycling conditions were identical for both directions with 95°C 15:30 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... 40 min, 4°C) prior to incubation (1 h, 4°C) with 4.0 mL of Ni-NTA agarose (Qiagen) pre-equilibrated in the same buffer ...
-
bioRxiv - Molecular Biology 2020Quote: ... was added to the embryos and homogenized for 2 min at 40 Hz using a Tissue Lyser LT (Qiagen) and 5 mm stainless steel beads (Qiagen) ...
-
bioRxiv - Microbiology 2021Quote: ... and subjected to real-time RT-PCR for viral RNA quantitation [40] using QuantiTect Probe RT-PCR Kit (Qiagen) and QuantStudio 5 (Thermo Fisher Scientific) ...
-
bioRxiv - Bioengineering 2022Quote: Nuclei were isolated from differentiating white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 uL of Tris-Hydrochloride (Sigma ...
-
bioRxiv - Bioengineering 2021Quote: Nuclei were isolated from white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 ul of Tris-Hydrochloride (Sigma ...