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Citations for Qiagen :
101 - 150 of 682 citations for Adenovirus Type 3 Particles Wild type since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: DNA from both types of samples was extracted using a commercially available kit (DNeasy Blood & Tissue Kit, QIAGEN) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
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bioRxiv - Immunology 2021Quote: The indicated cell types were harvested and stored at −80°C until RNA was isolated using RNeasy kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Thirty-three RNA samples were extracted from three biological replicates of each of 11 diverse tissue types of A188 with RNeasy Plant Mini Kit (Qiagen) (Supplementary Table 10) ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying the response elements for type I (ISRE - #CLS-008L-1) or type II (GAS - #CLS-009L-1) upstream of firefly luciferase was purchased from Qiagen. Twenty-four hours post plating ...
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bioRxiv - Developmental Biology 2020Quote: ... ∼100 ng of total RNA from each cell type was used as input to generate miRNA sequencing libraries using a QIAseq miRNA library kit (Qiagen). Single-end 100 bp reads (1×100 ...
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bioRxiv - Immunology 2021Quote: ... cDNA was quantified using 96-well PCR array analysis on a PAMM-150ZA plate (Cytokines & Chemokines) and PAMM-016ZA plate (Type I Interferon Response) (both Qiagen). Quantitative real time-PCR (QRT-PCR ...
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bioRxiv - Cancer Biology 2022Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA)(42) (Qiagen) and Genomic Regions Enrichment of Annotations Tool (GREAT)(43) ...
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bioRxiv - Molecular Biology 2020Quote: DNA extraction was done from 1 ml of the different sample types using QIAamp DNA mini kit (Qiagen, Hilden, Germany), as per the vendor’s instructions with slight modifications ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... with tissue homogenization being performed using a rotor stator type tissue homogenizer (ProScientific Bio-Gen PRO200 Homogenizer; Multi-Gen 7XL Generator Probes) in RLT Buffer (Qiagen). RNA quality was assayed using a nanodrop ...
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bioRxiv - Molecular Biology 2023Quote: ... cf-DNA quantified from various plasma or serum types using either MitoQuicLy or a silica-based membrane column DNA extraction kits (DNeasy, Qiagen). A total of 50 samples were analyzed (5 biofluids ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA from HeLa cells transfected with either wild-type PADI2 (WT) or mutant PADI2 (with L642A, or L642A/W161A, or L642A/W161A/T159A) was extracted using RNeasy (Qiagen) according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Pathway analysis of genes adjacent to identified tissue and cell-type specific methylation blocks was performed using Ingenuity Pathway Analysis (IPA) (Qiagen) (90).
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Microbiology 2019Quote: ... was used to amplify species specific 16S rRNA genes in order to identify and to quantify the relative abundance of 45 types of gut microbiota according to the manufacturer’s instructions (Qiagen, Hilden Germany) (Supplementary table 1) ...
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bioRxiv - Genetics 2023Quote: ... TPOX and FGA) and the Amelogenin (AMEL) gender-identification locus (24,25) using the Type-it Microsatellite PCR kit (Qiagen Gmbh, Hilden). The three multiplex PCRs and their respective primer pairs are given in Supplementary Table 1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The complete set of 15 loci were amplified in four separate 15 μL multiplex-PCR reactions containing 7.5 μL of 2X Master Mix (Type-It Microsatellite PCR kit, Qiagen, Venlo, Netherlands), 0.015-0.15 μL of unlabeled forward primer with tail (10 μM) ...
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bioRxiv - Biochemistry 2022Quote: Total RNA from cardiomyocytes was obtained on day 6 post adenovirus infection using the RNeasy kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: Genomic DNA from wild type and exconjugants from the indicated strains were isolated from liquid cultures using the Blood and Tissue DNeasy kit (Qiagen, Hilden, Germany) after pretreating the cells with 20 mg/mL lysozyme for 0.5–1 h at 30 °C ...
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bioRxiv - Microbiology 2021Quote: ... All amplifications of the rps10 locus were carried out using the QIAGEN Type-it Mutation Detect PCR Kit (QIAGEN, 206343, Valencia, CA). Multiplex PCR reactions were performed in 35 µL with 14 ng template DNA and 1 X final buffer concentration ...
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bioRxiv - Genetics 2023Quote: ... amplifications were performed in reaction volumes of 25 μL using a reaction mix containing 1x Type-it Multiplex PCR Master Mix (Qiagen, Gmbh, Hilden), 0.2 μM of each forward and reverse primer pair ...
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bioRxiv - Developmental Biology 2022Quote: ... Lentiviral particles were produced using Attractene (301005, Qiagen) in human embryonic kidney (HEK ...
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bioRxiv - Molecular Biology 2020Quote: DNA was extracted from each sample type using three different approaches: 1) Qiagen (QIAamp® DNA minikit or QIAamp® DNA Stool Mini Kit, Qiagen Inc., Germantown, MD, USA); 2 ...
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bioRxiv - Genomics 2021Quote: ... HMW gDNA was captured by magnetic particles (Qiagen MagAttract Suspension G), and then the magnetic particles with HMW gDNA was washed in wash buffer and eluted in EB Buffer (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... or Cignal Lenti Negative Control (CLS-NCL) lentiviral particles purchased from Qiagen. An equal volume of Bright-Glo™ Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2019Quote: Viral RNA was isolated from virus particles with RNeasy-Kit (Qiagen, Valencia, CA). Access RT-PCR kit (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... #3: 5’-AAGCATCGATAGGTAAGTTGA-3’ (SI03130008, Qiagen); #4 ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP36-3 (5’-CCGCAUCGAGAUGCCAUGCAU-3’; Qiagen), si-USP36-4 (5’-UUCCUUGUGAGUAGCUCUCAA-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... si-USP10-3 (5’-AACACAGCUUCUGUUGACUCU-3’, Qiagen), si-USP10-4 (5’-AAGAACUAGUUCUUACUUCAA-3’ ...
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bioRxiv - Microbiology 2020Quote: ... 107 viral particles/ml) was extracted using the DNeasy PowerSoil Kit (Qiagen, Germantown, MD). As the samples were liquid ...
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bioRxiv - Microbiology 2021Quote: ... Third shoot leaves from wild and mesocosm plants were placed in DNeasy PowerSoil C1 solution (Qiagen) and sonicated for 3 minutes at 1.2 kHz in a sonicator bath ...
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bioRxiv - Cell Biology 2021Quote: ... E2D2/3 (5’-AACAGUAAUGGCAGCAUUUGU-3’) and E2D4 siRNAs (5’ – CCGAAUGACAGUCCUUACCAA-3’) all from Qiagen, USA(83).
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bioRxiv - Neuroscience 2023Quote: ... fluorescently labeled GC particles were collected in RLT (Allprep DNA/RNA Micro Kit #80284, Qiagen) supplemented with 10% b-Mercaptoethanol.
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bioRxiv - Cancer Biology 2023Quote: ... HGF (5’-CTCACACCCGCTGGGAGTAC-3’, 5’-TCCTTGACCTTGGATGCATTC-3’), STAT3 (5’-CTTTGAGACCGAGGTGTATCACC-3’, 5’-GGTCAGCATGTTGTACCACAGG-3’) and B-Actin Primer Set (Qiagen, QT00095431). After preparing master mixes samples were prepared in quadruplicate in a 96-well Reaction Microplates (Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of gDNA was extracted from WILD-seq transduced cells (Blood&Cell Culture DNA Kit, Qiagen) and the barcode amplified by PCR using primers containing Illumina-compatible adapters (gDNAWhitelist_Fwd/Rev) ...
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bioRxiv - Microbiology 2023Quote: ... Viral RNA was isolated from the particles using QIAamp DSP Viral RNA Mini Kit (Qiagen, USA), and treated with RNAse-free DNAse I (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: The MS2 RNA was purified from the phage particles using QIAamp Circulating Nucleic Acid Kit (Qiagen, Germany) accordingly to the manufacturer’s protocol and stored at −80°C.
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bioRxiv - Pathology 2022Quote: DNA was extracted from the 200 μl concentrated particles solutions using the DNeasy plant mini kit (Qiagen). Two 3-mm and twenty 2-mm glass beads were added to the particles solutions together with 400 μL of lysis buffer and 4 μl RNase ...
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bioRxiv - Microbiology 2022Quote: ... viral particles were lysed and total RNA was extracted using QIAamp viral RNA mini kit (Qiagen, Switzerland) following manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... seeds and stems of wild castor and extracted total RNA using the QIAGEN RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany). In addition ...
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bioRxiv - Evolutionary Biology 2022Quote: We collected blood samples non-destructively from mistnetted wild birds (Table S6) and extracted DNA using DNeasy Tissue Extraction Kits (Qiagen) or standard phenol-chloroform protocol45.
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bioRxiv - Genomics 2019Quote: ... high molecular weight DNA was extracted from the tissue of a wild caught individual from Scotland with the Dneasy Blood and Tissue Kit (Qiagen) following the manufacturer’s protocol with additional Riboshedder and phenol-chloroform clean-up ...
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bioRxiv - Cell Biology 2019Quote: ... KIF4A siRNA 5’-CAGGTCCAGACTACTACTC-3’ against the 3’-UTR was obtained from QIAgen, and an optimised siRNA pool for KIF22 (KID ...
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bioRxiv - Physiology 2020Quote: ... Erythrocytes were lysed for 3 minutes with 3 ml of EL buffer (Qiagen). After Fc blocking for 20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... 3’mRNA-seq libraries were prepared using QIAseq UPX 3’ Transcriptome Kit (QIAGEN). In brief ...
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bioRxiv - Biophysics 2021Quote: The SARS-CoV-2 RNA was isolated from virus particles with the QIAmp viral RNA mini kit (Qiagen - USA) and reversely transcribed to cDNA with the High-Capacity Reverse Transcription Kit (Thermo - USA) ...
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bioRxiv - Microbiology 2022Quote: ... viral RNA was extracted from the suspension (200 μl) of concentrated particles using RNeasy Plant Mini Kit (Qiagen, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: We extracted genomic DNA from individual wild-caught adult Aedes in Colombia using Genetra Puregene Tissue Kits (Qiagen, Valencia, CA, USA), constructed barcoded libraries for sequencing using KAPA HyperPrep kits (Roche Sequencing ...
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bioRxiv - Cell Biology 2019Quote: siRNAs used were as follows: Kif5b#3 (target sequence 5′-CAGCAAGAAGTAGACCGGATA-3′; Qiagen SI00176050), Kif5b#4 (target sequence 5′-CACGAGCTCACGGTTATGCAA-3′ ...
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bioRxiv - Cell Biology 2021Quote: ... siMad2 (5’-CUGAAAGUAACUCAUAAUCUA -3’) (Qiagen). For transfection of the scFv or scFvC plasmids ...