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Citations for Qiagen :
1401 - 1450 of 10000+ citations for Mouse T Cell Surface Glycoprotein CD8 Beta Chain CD8B ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Genomic DNA from CRISPR-on HEK293T cells and control cells were purified using a AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Then ...
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bioRxiv - Microbiology 2020Quote: ... total DNA from 2×106 of Burkitt cells was extracted by the Blood & Cell culture DNA mini kit (Qiagen #13362). For extracellular viral DNA extraction ...
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bioRxiv - Cell Biology 2021Quote: ... After the last irradiation cells were given 24 hours to recover and gDNA was isolated using the Blood & Cell Culture DNA Midi Kit (Qiagen) according the manufacturers protocol (DNA content of MRC-5 cells was estimated at 10 pg per cell ...
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bioRxiv - Cell Biology 2021Quote: Total mRNA was isolated from HEK293 (2×106) and Jurkat cells (4×106) parental and MCU-KO cell lines using the RNeasy Mini Kit (Qiagen). Isolated RNA was then analyzed using a NanoDrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from tissues (CC) or cells (microglial GFP+ cells sorted by FACS) using the RNeasy Mini Kit (Qiagen). After reverse transcription using Superscript First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... shRNA expressing cells were isolated by fluorescence activated cell sorting at passage doubling 1 and 15 followed by genomic DNA isolation with Gentra Puregene cell kit (Qiagen). Sequencing libraries were prepared by subjecting 18 μg (1.2 μg x 15 reactions ...
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bioRxiv - Immunology 2022Quote: Clonal naïve GMP and neutrophil total RNA was extracted from cells at 5×106 cells/ml using the Qiagen RNeasy Plus Mini Kit (Qiagen) with DNaseI treatment (RNase free DNase Set ...
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bioRxiv - Immunology 2019Quote: Total RNA was extracted from sputum cell pellets or bronchial epithelial cell lysates stored in RLT buffer (RNeasy kit, Qiagen) and 2μg was used for cDNA synthesis (Omniscript RT kit) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then harvested and subjected for genomic DNA isolation using “Blood & Cell Culture DNA Mini Kit” (Qiagen, Hilden, Germany). Reactivation of LCLs was determined by real-time quantitative PCR (qPCR ...
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bioRxiv - Immunology 2020Quote: RNA from isolated fresh B cells and B cells activated with LPS/IL-4 was extracted using a RNeasy kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from wild-type and ΔTMPRSS2 293T cell lines using the Puregene cell kit according to the manufacturer’s instructions (Qiagen, #158388). Co-transfection of pJC144 (guide 1 ...
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bioRxiv - Microbiology 2020Quote: ... or experimental siRNA 72 h after transfection (approximate cell concentration was 1.6×105 cells/ well) were harvested and lyzed using the QIAshredder Kit (Qiagen; 79656). mRNA was harvested using the RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Molecular Biology 2020Quote: ... was extracted from the top (5.61 million cells) and bottom (4.87 million cells) fractions using the QIAamp DNA mini kit (QIAGEN cat.#51304). Fixed cells were decrosslinked by overnight incubation in 200 µL of PBS with added Proteinase K and 200 µL buffer AL (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: RNAs were prepared from purified D0 cells and from D7 to D14 populations isolated by flow cytometric cell sorting using an RNeasy Plus kit (Qiagen), and then depleted of rRNAs and processed for RNA-Seq library construction using a TruSeq Stranded mRNA Library Prep Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: Total RNA (5×106 cells) was extracted from FEA or CRFK cell lines by using the RNeasy Plus Mini Kit (Qiagen). RNA from FEA ...
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bioRxiv - Immunology 2021Quote: Human B cells harvested post gene-editing or cell sorting were lysed for RNA extraction by the RNeasy Micro Kit (74004, Qiagen). Primers used for reverse transcription and library amplification were modified from previous29 ...
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bioRxiv - Genomics 2021Quote: ... DNA was isolated from thawed APOE cell lines by Bioneer A/S using the Blood & Cell Culture DNA Mini Kit (Qiagen). For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) isolation from bulk transfected cells and clonal cell lines was performed using the DNeasy Blood and Tissue kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNA was isolated from approximately 107 cells grown to a density of 106 cells/ml using the RNAeasy Mini Kit (Qiagen), including an on-column DNase I treatment (Qiagen) ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from 5 million cells per cell line using the Qiagen AllPrep DNA/RNA extraction kits (Qiagen, 80004). Nucleic acid quantification and quality detection were performed and RNA sequencing libraries were generated via Illumina Stranded mRNA prep ...
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bioRxiv - Microbiology 2022Quote: DNA was purified for sequencing from cells infected in six well plates using a blood and cell culture DNA mini kit (Qiagen). Samples were sequenced at the Northwestern University Genomics Core Facility using NextGen Illumina HiSeq SR500 sequencing ...
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bioRxiv - Immunology 2023Quote: ... Genomic DNA was extracted from puromycin selected SUDHL4 cells and untreated WT cells using the QIAamp DNA Blood Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the cell culture supernatants were collected and cells were lysed using RLT Buffer from the RNeasy Mini Kit (#74106; Qiagen).
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from 1 million cells of each cell line using the QIAamp®DNA Micro Kit (QIAGEN) following the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondria isolation from HEK293T cells was performed after 24 hours of cell transfection using the Q proteome Mitochondria Isolation Kit (QIAGEN). Cell lysis and mitochondria homogenisation were conducted as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... we used 6 mL of 55 °C Cell Lysis Solution from The Gentra Puregene® Cell and Tissue Kit (Qiagen) with 0.1 mg/mL proteinase K and 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: Bulk-RNA free of genomic DNA for RT-qPCR analysis was extracted from cell pellets (≤ 2 x 106 cells) using the RNeasy plus spin-column purification kit (Qiagen) and performed using the Qiacube liquid handler (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from Library-Diverse was isolated by Blood & Cell Culture DNA Maxi Kit (Qiagen; 30 million cells per condition). Genomic DNA from the TRIP library editing experiments was isolated by DNeasy Blood & Tissue Kit (4 million cells per condition) ...
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bioRxiv - Bioengineering 2023Quote: ... phenol-free total RNA was extracted from cells directly in cell culture wells using RNeasy Plus Micro Kit with gDNA eliminator columns (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Cells collected from either 3D culture organoids or sorted cells were lysed in RLT buffer from RNeasy kit (Qiagen, 74106). RNA samples were isolated using the same kit following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA Extraction and Purification: Genomic DNA was extracted from 5 M cells using the Gentra Puregene Cell Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Genomic DNA from freshly sorted single cells was extracted and amplified using the REPLI-g Advanced Single Cell kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The DNA was extracted from the FACS sorted cells and whole genomes were amplified using multiple displacement amplification technique (MDA) with the REPLI-g Single Cell kit (Qiagen) by following the manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cell pellets containing at least 1e6 cells/pellet using the DNeasy Blood and tissue kit (Qiagen), followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... Samples were then centrifuged and plasma isolated and stored at -20°C prior to testing in the QuantiFERON IFNγ ELISA (Qiagen). Acceptance criteria for the assay specified by manufacturers were always met.
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bioRxiv - Immunology 2020Quote: SARS-CoV-2 ELISA was developed in-house using His tagged proteins bound on Ni-NTA HisSorb Strips or Plates (Qiagen). ELISA assay on mouse sera were performed with His-tagged SARS-CoV-2 full length Spike protein (produced in baculovirus ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: DNA was isolated from tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valenica, CA, USA). Genomic DNA was digested with BamHI-HF (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2021Quote: ... Total genomic DNA was extracted from each ked lysate and blood (camel, mouse, and rabbit) using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from the tail tip of a wild-type C57BL/6J mouse using the DNEasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from blood-free cranial lobes of the right mouse lung using the RNeasy Tissue Mini Kit (Qiagen, Hombrechtikon, Switzerland). Isolated RNA was reverse-transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... Parasite genomic DNA was purified from the blood of a transfer mouse using the Qiagen QIAamp DNA Blood Kit (Qiagen, Cat# 51106), and genotyping PCR was performed to assess integration into the target locus ...
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bioRxiv - Immunology 2022Quote: ... Three hundred cells from three to six mouse per condition were sorted directly into 25 μl TCL buffer (Qiagen, 1031576) supplemented with 1%β-mercaptoethanol at single cell precision using a BD FACS Aria II instrument ...