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Citations for Qiagen :
1401 - 1450 of 1985 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Synthetic Biology 2023Quote: ... The organs were weighed and lysed in 1 mL Triton X-100 solution (0.1% in PBS) using a TissueLyser II (QIAGEN, Germany) according to the manufacturer protocol ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... 45 cycles at 95° for 15 sec and 60° for 1 min were performed in the Rotor-Gene Q real-time PCR cycler (Qiagen). The numbers of copies of the RdRP gene in the samples were calculated using an RdRP cDNA standard curve.
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... we added 1 µl of the spike-in mix provided in the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN) to the tube ...
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bioRxiv - Pathology 2024Quote: ... liver was homogenized in 500uL of 3:1:6 isopropanol:water:ethyl acetate containing internal standard in ceramic bead tubes (Qiagen #13113-50) using the TissueLyzer II (Qiagen #9244420) ...
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bioRxiv - Microbiology 2024Quote: ... The DNA of the 20 Jan and 3 Mar 2021 samples (1/3 pellet) was extracted with the DNeasy UltraClean Microbial Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and homogenized via sonication at 20 kHz for 1 min before RNA isolation was performed using a RNeasy Mini Kit (Qiagen). For qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was subjected to immobilized metal affinity chromatography using a 1 mL Ni-NTA agarose column (Qiagen, 30210) equilibrated with binding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were incubated for 15 min at 60°C and then terminated by adding 1 μL 5 M NaOH to degrade RNA and heating at 95 °C for 5 min followed by neutralization with 1 μL 5 M HCl and one round of MinElute column clean-up (Qiagen). The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... The solubilized supernatant was collected by another round of ultracentrifugation (200,000 × g, 1 h) and applied to a Strep-Tactin resin column (Qiagen, USA) equilibrated with buffer A containing 0.025% (w/v ...
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bioRxiv - Immunology 2024Quote: Genomic DNA samples were prepared from heparinized peripheral blood or 1×106 Jurkat cells following the instructions of the QIAamp DNA Blood Mini kit (#51104; QIAGEN). Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights) ...
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bioRxiv - Plant Biology 2024Quote: ... Fragments between 200 and 400 bp were extracted from a 1% agarose gel with QIAquick Gel Extraction Kit (Qiagen, Netherlands). The final library was sequenced as 85 bp long single-end reads on a NextSeq™ 550 or NextSeq™ 2000 (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were then analyzed on 1% agarose gel and purified using the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The primer extension reaction was performed in a volume of 20 μL with a 32P-labeled primer (complementary to galM of the galactose operon mRNA; Table S1) and 1 U of Taq polymerase (Qiagen) (X et al ...
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bioRxiv - Microbiology 2024Quote: ... The footpad was ground in 1 mL of DMEM containing 2% FBS with steel beads using a Tissue-Lyser II (Qiagen) and debris was clarified by centrifugation at 8,000 x g for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Illumina sequencing-compatible Unique Dual Index (UDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2x250bp reads.
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bioRxiv - Microbiology 2024Quote: ... The resulting cells were resuspended in 1 ml of PBS and genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). Genomic DNA (∼10 µg ...
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bioRxiv - Microbiology 2024Quote: We harvested SARS-CoV-2 RNA by collecting 25 μL of cell culture medium at various time points post infection and stored in viral lysis buffer with RNA supplements (AVL buffer supplemented with 1% carrier RNA; Qiagen). We extracted RNA using a QIAamp viral RNA mini kit (Qiagen) ...
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bioRxiv - Immunology 2024Quote: ... and approximately 1 µg of isolated RNA was converted to cDNA using a RT2 First Strand Kit (Qiagen, Hilden, Germany). qRT-PCR was performed using a SYBR Select Master Mix (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 1 ml of this culture using the Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany), adhering to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were titered and 1 mL was allocated for DNA extraction using DNeasy Blood & Tissue Kit (Qiagen catalog number 69504). qPCR was performed in triplicate on 1:10 dilutions of the extracted DNA with TaqMan Real-Time PCR Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... The effect of phage on the key QSS was quantified in real-time using 1 µL of the cDNA with QuantiTect SYBR Green PCR Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Faeces was collected in pre-weighed tubes containing 1 ml PBS and homogenised with a steel ball for 2 minutes at 25 Hz using a Tissue-Lyser (Qiagen). Mice were euthanised at indicated time-points ...
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bioRxiv - Microbiology 2024Quote: ... Cell lysis was performed by bead beating at 50 Hz for 1 min using a TissueLyser LT (QIAGEN, Hilden, Germany). Sequencing was performed by Macrogen Inc ...
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bioRxiv - Immunology 2024Quote: ... Illumina sequencing-compatible Combinatorial Dual Index (CDI) adapters were ligated onto the pools using the QIAseq 1-step amplicon library kit (Qiagen). Library QC was performed using Qubit and Tapestation and sequenced on Illumina MiSeq platform to generate 2×250bp paired-end reads.
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from FACs-sorted PFA-fixed cells (populations as shown in Supplementary Fig. 1) using the miRNAeasy FFPE kit for microRNA extraction (Qiagen). Total RNA integrity was assessed using the Agilent Bioanalyzer ...
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bioRxiv - Genomics 2024Quote: ... Bxb1 integrase-edited Rep 1 and Rep 2 K562attB gDNA genomic DNA was extracted using DNeasy blood and tissue kit (Qiagen) and samples library preparation and Illumina short read sequencing with a target of 60x genomic coverage ...
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bioRxiv - Immunology 2024Quote: Cell-free HIV-1 RNA was isolated from 50 μl plasma using a QIAamp Viral RNA Mini Kit (QIAGEN, 52906) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR product was treated with 40U Dpn I for 1 hour and was purified by QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... The reaction mixture was incubated at 37°C for 1 hour followed by purification using QIAquick PCR Purification Kit (QIAGEN). The purified DNA was quantified by Qubit dsDNA BR kit (Invitrogen).
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bioRxiv - Developmental Biology 2024Quote: ... 5 µl of each PCR product was run in a 1% agarose gel and the remaining 35 µls were purified using the QIAquick PCR Purification Kit (Qiagen) before being sent for sequencing (sequencing primers ...
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bioRxiv - Microbiology 2024Quote: ... A 1 μg of total RNA of each sample was reverse-transcribed to cDNA using RT2 first strand kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... semimature SP thymocytes were isolated on BD FACSAria II and BD FACSAria Fusion cell sorters directly into RLT + 1% beta-mercaptoethanol (BME) buffer (Qiagen). Libraries were generated by the Emory Integrated Genomics Core (EIGC ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were separated on a 1% agarose gel and bands were excised using a QIAquick gel extraction kit (Qiagen). The purified PCR bands were cloned using the NEB PCR cloning kit following manufacturer’s instructions and transformed colonies were screened using colony PCR ...
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bioRxiv - Microbiology 2024Quote: ... and 0.5 mm (0.3 g) Zirconia beads and 1 mL of InhibitEx buffer from the QIAmp Fast DNA Stool Mini Kit (QIAGEN #51604) in a 2 mL tube ...
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bioRxiv - Molecular Biology 2024Quote: ... and HET(2)) and four biological replicates of PA-1 (WT and HET) cells was extracted using miRNeasy Mini Kit (Qiagen) followed by on-column DNAse digestion ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed twice with 1× PBS and total DNA was extracted at 6 hpi with a DNeasy Blood/Tissue DNA mini kit (Qiagen) and total DNA yield was measured by a nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription of 1–2 μg of RNA for cDNA synthesis was carried out using the Omniscript RT Kit (Qiagen).
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bioRxiv - Neuroscience 2024Quote: Total RNA from isolated astrocytes or newborn neurons and MSP-1 differentiation induced astrocytes was isolated using RNeasy Mini Kit (Qiagen).1ng (isolated astrocytes and newborn neurons ...
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bioRxiv - Physiology 2024Quote: ... The digest was run on an 1% Agarose gel (TAE buffer, 0.25 µg/ml EtBr) and the the QIAquick Gel extraction kit (Qiagen, 28704) was used to extract the pLV6 backbone according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... Cells were briefly vortexed for 1 minute and homogenized by loading the lysate into a QIAshredder spin column (QIAGEN, #79656) and centrifuging for 2 minutes at full speed ...
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bioRxiv - Neuroscience 2024Quote: ... After combining the two aqueous layers 1 equal volume 70% EtOH was added and the solution was added to an RNAeasy column (QIAgen). The RNA was washed with RW1 buffer ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 ml of phenol:chloroform:isoamyl alcohol (25:24:1) was added and samples were bead beaten using a TissueLyser LT (Qiagen, Hilden, Germany) for 10 minutes at 25 Hz ...
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bioRxiv - Cancer Biology 2024Quote: ... we isolated genomic DNA from 2x106 cells (ensuring an average of 1 000x representation for each gRNA) with the AllPrep DNA/RNA Mini Kit (Qiagen), followed by PCR amplification of the gRNAs using forward (5’-CGATACAAGGCTGTTAGAGAGATA-3’ ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from root tissue 1 cm above the root tip using the RNeasy plant mini kit (Qiagen). Extracted RNA was DNase treated and converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs) ...
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bioRxiv - Biochemistry 2024Quote: ... Nanobody library DNA fragments were digested with the restriction enzymes FseI and Nsil at 37°C for 1 hour and purified using gel extraction (Qiagen). Similarly ...
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bioRxiv - Genomics 2024Quote: ... and the samples from the Democratic Republic of Congo (n = 1) were processed using the QIAsymphony DSP Virus/Pathogen Kit (QIAGEN) with modifications ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was extracted from input and IP samples by incubation with 1 mL Trizol at room temperature for 5 minutes and then RNeasy Lipid Tissue Mini Kit (Qiagen) via the manufacturer’s protocol with on-column DNase treatment (Roche) ...