Labshake search
Citations for Qiagen :
1351 - 1400 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or DNeasy Blood & Tissue Kit (Qiagen). RNA integrity was assessed by the Agilent 2100 Bioanalyzer and quantified by NanoDrop ND-1000 spectrophotometer (Thermo-Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... AllPrep DNA/RNA Mini Kit (Qiagen) or the Gentra Puregene Blood Kit (Qiagen) ...
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Cited4a limits cardiomyocyte dedifferentiation and proliferation during zebrafish heart regenerationbioRxiv - Developmental Biology 2024Quote: ... and RNeasy micro kit (Qiagen #74004). For qPCR ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by RNeasy Mini kit (Qiagen) clean-up following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... a DNA extraction kit from Qiagen (DNeasy Blood & Tissue Kits, Qiagen, USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... a DNA extraction kit from Qiagen (DNeasy Blood & Tissue Kits ...
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bioRxiv - Evolutionary Biology 2024Quote: ... AllPrep DNA/RNA Micro kit (Qiagen) kit was used to extract genomic DNA and RNA from them according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... RNeasy Lipid Tissue kit (Qiagen, CA) was used with on-column DNase digestion as detailed in the kit user guide ...
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bioRxiv - Neuroscience 2024Quote: ... and a RNeasy mini kit (Qiagen). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... or RNeasy Mini Kit (Qiagen, #74104) and 1000 ng of total RNA reverse transcribed using RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... RNeasy Mini Kit (Qiagen, Hilden, Germany) was used and RNA samples were converted to cDNA with RevertAid H Minus Reverse Transcriptase kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was then amplified using qPCRBio SyGreen Mix (PCR Biosystems, Wayne, PA, USA) and QuantiTect Primer Assays (Qiagen, Hilden, Germany) for ACE2 (Hs_ACE2_1_SG ...
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bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
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bioRxiv - Cell Biology 2020Quote: ... qPCR reactions were prepared with RT2 SYBR master mixes and RT2lncRNA qPCR assays for DR5-AS (Qiagen Cat., LPH15855A-200), CAMTA-AS (Qiagen Cat. ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time qPCR reactions were run using the MESA Green qPCR MasterMix Plus for SYBR Green assay (Eurogenetec, RT-SY2X-03+WOU) on the Rotor-Gen Q device (Qiagen). Each sample was tested in triplicate ...
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bioRxiv - Systems Biology 2022Quote: ... Real-time qPCR reactions were run using the MESA Green pPCR MasterMix Plus for SYBR Green assay (Eurogenetec, RT-SY2X-03+WOU) on the Rotor-Gen Q device (Qiagen). Each sample was tested in triplicate ...
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bioRxiv - Neuroscience 2021Quote: ... and rplp0 (all QuantiTect Primer Assays: 18s Cat. No. QT02448082, gapdh Cat. No. QT01658692, hprt1 Cat. No. QT00166768, rplp0 Cat. No. QT00249375; Qiagen). We analyzed the relative expression of the following genes of interest ...
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bioRxiv - Immunology 2020Quote: ... “Healthy controls” defined as HIV-negative (negative HIV ELISA and undetectable HIV viral load) and with latent TB infection (LTBI) as indicated by a positive QuantiFERON-TB Gold Plus (QFT-Plus) assay (Qiagen) (IFN-γ > 0.35 IU/mL after subtracting background) ...
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bioRxiv - Molecular Biology 2020Quote: ... catalysis was measured using a previously described assay (22) in which hexahistidine-tagged PARP-1 was immobilized on Ni(II)-NTA-coated plates (Qiagen) in the presence of NAD+/biotinylated-NAD+ (Trevigen) ...
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bioRxiv - Pathology 2020Quote: ... and the RT product was used for detection of miR-574-5p/3p and control RNA Snord68 using the miScript Primer Assay (Qiagen).
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bioRxiv - Immunology 2020Quote: ... the miScript Universal Primer was used alongside miR specific miScript primer assays (miR-206 cat. no. MS00001869 and U6 cat. no. MS00033740; Qiagen).
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bioRxiv - Cell Biology 2022Quote: ... hsa-miR-486 and hsa-Let-7g were quantified by relative quantification using Qiagen LNA based SYBR green detection method (miRCURY LNA miRNA PCR assay-Qiagen). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA generated after performing reverse transcription of RNA product was subjected to quantitative PCR (qPCR) analysis using gene-specific predesigned primers (RT2 qPCR Primer Assay, Qiagen), listed in Supplementary Table S4 ...
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bioRxiv - Microbiology 2024Quote: ... with ReadyMade PrimeTime primers for SPI1 (Integrated DNA Technologies Inc, USA) and RT2 qPCR Primer Assay for Human GAPDH (cat# PPH00150F-200, Qiagen). Expression was quantified using ABI Sequence Detection software compared to serial dilutions of an SPI1 or GAPDH synthetic sequence gBlock (Integrated DNA Technologies Inc ...
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bioRxiv - Cell Biology 2024Quote: ... Actin was used as a housekeeping gene to normalize the amount of viral RNA by using the qPCRBIO SyGreen mix HI-ROX (PCR Biosystems) together with QuantiTect primer assay (QT01680476, Qiagen). All Quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... Adequate sensitivity of the assays was assessed as described earlier using (un)methylated DNA standards from the EpiTect PCR Control DNA Set (Qiagen).
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bioRxiv - Neuroscience 2023Quote: ... Adequate sensitivity of the assay was initially confirmed using methylated and unmethylated DNA standards from the EpiTect PCR Control DNA Set (Qiagen).
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bioRxiv - Genomics 2023Quote: ... The bead-bound gDNA was isothermally amplified for 3 hours at 30 °C then 10 minutes at 65 °C using a miniaturised (1/5 vols) Repli-g Single-Cell assay (Qiagen). The amplified gDNA was cleaned up with 0.8 × vols Ampure XP and 80 % ethanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Amplification and sequencing primers for pyrosequencing analysis are listed in Extended Data Table 14 and were designed using PyroMark Assay Design Software (Qiagen). PCR reaction consisted of 1.5 µl of BST samples or control DNA ...
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bioRxiv - Neuroscience 2024Quote: ... The expression levels of the endogenous miRNAs and spike-ins were examined using the miRCURY LNA miRNA PCR Assay (QIAGEN), using primers designed for the Homo sapiens genome and validated for the Mus musculus genome (Table 4) ...
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bioRxiv - Microbiology 2024Quote: ... Mouse GAPDH or human actin mRNAs were used as a reference and detected by QuantiTect primer assay (GAPDH; QT01658692, Actin; QT01680476, Qiagen) and the qPCRBIO SyGreen mix Hi-ROX (PCR Biosystems) ...
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bioRxiv - Immunology 2024Quote: ... the cDNA of 5 animals in each experimental group were pooled and mixed with the PCR master mix buffer (Rt2 Syber Green ROX qPCR Primer Assay, Qiagen) and analyzed on specific array plates (RT2 Profiler™ PCR Array Mouse Th1 & Th2 Responses-GeneGlobe ID - PAMM-034Z) ...
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bioRxiv - Biochemistry 2024Quote: ... Bace1 primers for PCR amplification and sequencing were generated according to PyroMark Assay Design software version 2.0 (Qiagen, Hilden, Germany) to analyze 5 CpG sites within the gene regulatory region ...
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bioRxiv - Cancer Biology 2024Quote: ... The target genes were selected based on their association with these transcription factors as identified through chromatin immunoprecipitation (ChIP) assays and from QIAGEN IPA databases ...
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bioRxiv - Molecular Biology 2021Quote: ... Total genomic DNA extraction was performed using a commercial genomic DNA kit (DNeasy Blood & Tissue Kit, Qiagen). Photoproducts were quantified using a pre-optimized procedure (Thierry Douki ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated using the RNeasy Mini plus Kit or the RNeasy Micro plus Kit (Qiagen). After determining RNA concentrations by spectrophotometry ...
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bioRxiv - Microbiology 2021Quote: ... blank DNA extraction kits (N=14)) controls using the DNeasy PowerLyzer Powersoil kit (Qiagen, Germamtown, MD, USA), with minor modifications to the manufacturer’s protocols as previously described [109 ...
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bioRxiv - Physiology 2020Quote: ... DNA was isolated using Qiagen MagAttract PowerMicrobiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) on the EpMotion 5075 (Eppendorf ...
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bioRxiv - Microbiology 2020Quote: ... RNA from Trizol samples was purified using a commercial kit (RNeasy® Mini Kit, Qiagen no. 74104) with additional on-column DNAse treatment (Qiagen no ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated using an RNeasy Mini Kit with the addition of RNase-free DNase kit (Qiagen) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cellular RNA was extracted using the QIAshredder kit and the RNeasy Mini kit (79654 and 74106, Qiagen). The cDNA was generated using High Capacity cDNA reverse transcript kit (4368814 ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated using an AllPrep DNA/RNA Mini kit or RNeasy Plus Micro kit (Qiagen). cDNA was prepared using the SuperScript III Reverse transcription Kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The RNA extraction process was referred to the instructions of the kit (RNeasy Mini kit, QIAGEN, Germany). The extracted RNA was detected by ultra-fine ultraviolet spectrophotometer (NanoDrop ...
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bioRxiv - Genetics 2021Quote: ... Genomic DNA was purified using the Xtreme DNA isolation kit (Isohelix) or DNeasy Blood & Tissue Kit (Qiagen). These kits were used to purify DNA from crude lysates or from tissue samples preserved in RNAlater solution (Sigma).
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bioRxiv - Microbiology 2021Quote: ... and viral DNA was extracted and purified using a commercial kit (DNeasy Blood and Tissue Kit, Qiagen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Amplified scFv PCR fragments were purified with Gel Extraction kit (Qiaquick Gel Extraction Kit, Cat# 28706, QIAGEN), and subjected to SfiI (Fast Digest SfiI ...
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bioRxiv - Microbiology 2022Quote: gDNA was purified from Bacteroidales using a DNeasy UltraClean Microbial Kit or DNeasy Blood & Tissue Kit (Qiagen). Up to 2 μg in 200 μL TE buffer was sheared by ultrasonication to an average of 3–5 kb with a Covaris S220 focused ultrasonicator using red miniTUBEs ...
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bioRxiv - Microbiology 2024Quote: ... an EZ1 extraction was performed using a Qiagen kit with EZ1 DNA Tissue Kit (Qiagen, Courtaboeuf, France) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested and total RNA isolated using an RNA isolation kit (RNeasy Mini kit, Qiagen, 74106). 8 µg of total RNA were treated with DNaseI and purified according to the manufacturer’s protocol (TURBO DNA-free kit ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using DNAeasy Power Soil Pro kit or DNAeasy PowerMax Soil kit (Qiagen, Kista, Sweden) for 47 mm and 142 mm filters ...