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Citations for Qiagen :
1351 - 1400 of 2106 citations for Enolase 1 Alpha enolase Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... Each PCR reaction was extracted from a 1% agarose gel and column purified using QG buffer (Qiagen), following manufacture instructions ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from cell pellets (1 million cells) using Allprep DNA/RNA mini kit (Qiagen). Whole genome libraries were sequenced 150x2 bp on a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA synthesis was carried out with 1 µg of RNA using the QuantiTect Reverse Transcription Kit (Qiagen) and subsequently diluted 1:25 in dH2O ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA (gDNA) was extracted from 1×106 PBMCs using the QIAamp DNA Blood Mini Kit (Qiagen) per manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared from 1 μg of total RNA using a QuantiTect™ Reverse Transcription kit (Qiagen) from random hexamer primers ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of 1 μg of RNA were reverse transcribed using a QuantiTect Reverse Transcription kit (Qiagen, 205314), and qPCR was performed with iTaq SYBR Green (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were confirmed with a 1% agarose gel and PCR purified (QIAquick PCR Purification kit, QIAGEN). The dsRNA was synthesized using a T7 reverse transcription reaction kit (MEGAscript ...
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bioRxiv - Genetics 2024Quote: DNA was isolated using 1 ml from whole blood using the QiAamp DNA blood Midi kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... The entire HTT exon 1 coding sequence was amplified using UltraRun® LongRange PCR Kit (QIAGEN, 206442) with 10% DMSO using the following PCR conditions ...
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bioRxiv - Biophysics 2020Quote: ... reaction mix (for PCR program details see Supplementary Table 1) and purified with QIAquick PCR Purification Kit (QIAGEN). The Fps1-mGFP strain was created by transforming the BY4741 background strain with 100 µL of the purified mGFP-HIS3 fragment with standard LiAc protocol to allow for homologous recombination 34 ...
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bioRxiv - Microbiology 2021Quote: ... This was purified by 1% agarose gel electrophoresis and gel extracted according to QIAquick gel extraction kit (QIAGEN) standard protocol ...
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bioRxiv - Genomics 2020Quote: Total RNA was purified from eleven samples Table 1 using the QiaAmp viral RNA mini kit (Qiagen – 52906) following viral inactivation at the MRCG at LSHTM containment level 3 facility ...
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bioRxiv - Microbiology 2022Quote: ... beat-beading tubes were filled 1/3 with beads and soaked overnight in 500 μl buffer RLT (Qiagen). Tubes were spun at full speed and excess buffer removed ...
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bioRxiv - Genomics 2019Quote: Total DNA was extracted from 1 ml of blood sample using DNeasy Blood and Tissue Kit (Qiagen, USA). The concentration of DNA ranged from 2.2 ng/ul to 9.7 ng/ul ...
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bioRxiv - Molecular Biology 2019Quote: ... or from 1 mL plasma using QIAamp Circulating Nucleic Acid Kit (QIAGEN, Cat. No.: 55114, QC for short) following the manufacturer’s guide ...
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bioRxiv - Molecular Biology 2019Quote: ... The peak fractions containing Esco2 were pooled and applied onto a 1 ml Ni-NTA Superflow column (Qiagen), equilibrated with 20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified from a 1% agarose gel using the QIAquick gel extraction kit (Qiagen, Inc.) and cloned in NdeI and XhoI sites of pET-22b (+ ...
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bioRxiv - Cell Biology 2019Quote: ... 1 ml of culture was harvested for RNA extraction using an RNeasy® Mini Kit (Qiagen, Hilden, Germany). DNA digestion was done to remove contaminating DNA in the solution before RNA clean up ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg total RNA was then used for first strand cDNA synthesis using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative real-time PCR was performed using iQ SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... containing Protease inhibitor cocktail (PIC, Complete, 1:25) and lysed at 4°C using a TissueLyser LT (Qiagen) on 50 Hz for 2 min ...
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bioRxiv - Neuroscience 2020Quote: ... 12,000 GFP+ nuclei were collected into cold Buffer RLT Plus supplemented 1:100 with β-mercaptoethanol (Qiagen, 74034) using the Sony SH800S Cell Sorter (purity mode ...
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bioRxiv - Cancer Biology 2020Quote: ... the first complementary strand was made from 1 µg total RNA using QuantiTect® reverse transcription kit (Qiagen). RT-PCR for the for gene expression analysis was carried out using the CFX-96 thermocycler (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... PCR products were purified and extracted from a 1% agarose gel using the QIAquick Gel Extraction Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total cellular RNA was extracted from Jurkat cells infected with HIV-1 using the RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... was homogenized in 1 ml phosphate-buffered saline (PBS) containing 0.1% Triton X-100 (Thermo) and 0.1% RNase A (Qiagen) using a BioMasher (Nippi ...
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bioRxiv - Plant Biology 2021Quote: ... frozen in liquid nitrogen and ground to fine powder (1 min at 30 Hz in a TissueLyzerII, Qiagen). Total protein was extracted by incubation with extraction buffer (6 µL/leaf disc ...
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bioRxiv - Biochemistry 2020Quote: ... For total RNA isolation a minimum of 1×107 PBMCs were processed using a RNeasy Mini Kit (Qiagen). First-strand synthesis of complimentary DNA from the resulting total RNA was prepared using the SuperScript™ IV First-Strand Synthesis kit (#18091050 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed with 1 µg of the isolated RNA using the QuantiTect Reverse Transcription Kit (Qiagen). mRNA expression levels of ARTD3 ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Bioengineering 2020Quote: ... RT-PCR amplifications were carried out using a Power SYBR Green RNA-to-CT 1 step kit (Qiagen) on a ViiATM Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2021Quote: We extracted DNA from avian tissue specimens (Supplementary Table 1) using the DNeasy Blood & Tissue Kit (QIAGEN, CA) and generated ddRADtags following the protocol of Peterson et al ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by phenol/chloroform extraction and 1 μg of RNA was reverse transcribed with Quantitect RT kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... First strand cDNA synthesis from 1 µg of total RNA was performed with QuantiTect Reverse Transcription Kit (Qiagen). Quantitative RT-PCR experiments were performed with Rotor-Gene-Q machine (Qiagen ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from cell pellets (<1×107 cells) using the RNeasy Mini kit (QIAGEN, cat. 74134). Cells were lysed with RLT buffer (with no ß-ME ...
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bioRxiv - Developmental Biology 2022Quote: ... miR-1 miRCURY LNA miRNA Power Inhibitor and miRCURY LNA miRNA mimic were obtained from Qiagen (Germantown, MD). miR-1 inhibitor (Hsa-miR-1-3p ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from Myc-CaPWT or Myc-CaPΔRb cells cultured in vehicle or JQ-1 (RNeasy, Qiagen). cDNA was synthesized using the iScript cDNA synthesis kit (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Fragments were extended with homology arms (Table 1) (1000ul NEBnext, Ta = 65, 30 cycles) and purified (Qiagen minelute) to prepare for electroporation ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA from MCF-7 cells (1×106 cells/well) was isolated using RNeasy Mini kit (74106; Qiagen), and 500ng cDNA was synthesized with random hexamers by reverse transcription (SuperScript III ...
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bioRxiv - Microbiology 2021Quote: ... the cell lysate was loaded by gravity flow onto a column containing 1 ml Ni-NTA resin (Qiagen) equilibrated with buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was synthesized using 1 µg of RNA per sample according to manufacturer’s instructions (Omniscript RT kit, Qiagen). Taqman assay probes (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... there were 1×106 ESCs culturing in 2iLIF condition for genomic isolation with QIAamp DNA Mini Kit (Qiagen). 500 ng of gDNA was used for bisulfite conversion followed by the instructions of EZ DNA Methylation-Gold Kit (Zymo) ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet, Qiagen) and incubated for 30 mins at 4 °C on a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
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bioRxiv - Genetics 2019Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... 1 million cultured 12878 cells using the Gentra Puregene Blood Kit following manufacturer’s instructions (Qiagen, Cat. No 158467). To generate longer DNA fragments (WμFL=150kb and longer ...
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bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
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bioRxiv - Cell Biology 2020Quote: ... collected in 1 mM EDTA in PBS and total RNAs were isolated using RNeasy Mini Kit (Qiagen, 74106) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... 120 µL of elution buffer of elution buffer II (10 mM Tris pH 8.0, 1 mM EDTA, 0.67% SDS) and 7 µL Proteinase K (Qiagen) was added ...
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...