Labshake search
Citations for Qiagen :
1351 - 1400 of 1933 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The homogenate was then centrifuged at full speed for 2 min in QIAshredder column (Qiagen, 79656). RNA was extracted from the flow-through using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genetics 2019Quote: ... DNA was separated by 2% agarose gel electrophoresis and mononucleosome bands were gel-extracted (Qiagen MinElute) and converted to genomic libraries using the Ovation Ultralow Library System V2 (NuGEN ...
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bioRxiv - Genomics 2021Quote: ... PCR products were run on a 2 % gel and purified using QIAquick Gel extraction kit (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... and 5 h time points were immediately mixed with 2 x volume of RNAProtect® (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... size selected on a 2% agarose gel and purified with a QIAquick Gel Extraction Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... for 1h at 37C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). In parallel ...
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bioRxiv - Immunology 2022Quote: ... Viral RNA was extracted from VSV-SARS- CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and the S gene was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... siRNAs used were RAPH1 siRNA #2 (Hs_RAPH1_2, SI00698642) and RAPH1 siRNA #5 (Hs_RAPH1_5, SI04300982) provided by Qiagen.
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bioRxiv - Immunology 2022Quote: ... genomic DNA from 2×107 cells was extracted by DNeasy blood & tissue kit (Qiagen, Cat.69504) and sgRNA cassettes were PCR amplified for illumina NGS 53.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 2-ml column of Ni-NTA-agarose (Qiagen, Hilden, DE) at a flow rate of 1 ml min−1 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
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bioRxiv - Microbiology 2023Quote: ... each cell cultures (4ml) are treated with 2 volumes of RNA Protect Bacteria reagent (Qiagen, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then treated with DpnI for 1-2 hr and then purified (QiaQuick, Qiagen) before use in library construction.
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bioRxiv - Genetics 2023Quote: ... PCR amplicons were resolved by 2% agarose gel electrophoresis and gel-purified (QIAGEN Gel Extraction Kit). Libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Cell Biology 2023Quote: ... Take 2 μg of template DNA and bisulfite treat according to the EpiTect Bisulfite Kit (QIAGEN) manual ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of purified genomic DNA was then bisulfite converted using the EpiTect Bisulfite Kit (Qiagen). PCR was performed on bisulfite-treated gDNA using HotStarTaq DNA polymerase (Qiagen) ...
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bioRxiv - Genomics 2023Quote: ... PCR 2 products were purified via gel extraction using the Qiaquick Gel Extraction Kit (Qiagen #28706X4) and prepared for sequencing as described in Illumina’s library denaturation and dilution manual ...
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bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Each qPCR reaction consisted of 5 μL of 2× QuantiNOVA SYBR Green PCR master mix (Qiagen), 0.5 μL of 10 μM forward and reverse primers each ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
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bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... Around 2 micrograms of DNA was used for DNA bisulfate conversion by EpiTect Bisulfate kit (QIAGEN). The converted DNA served as a template for amplification ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from iPSC-derived microglia (Supplementary Table 2) was extracted using RNeasy Plus kit (Qiagen). Library construction and sequencing were performed by Novogene ...
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bioRxiv - Biochemistry 2023Quote: PCR products were purified from 2% agarose gel and extracted by QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the Caco-2 cell lines using a RNeasy Mini Kit (QIAGEN SA) with a DNase I step to eliminate DNA contaminants ...
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bioRxiv - Immunology 2023Quote: DE genes between clusters 2 and 4 were fed to Ingenuity Pathway Analysis software from Qiagen. Enrichment pathways were sourced from Ingenuity core enrichment pathways.
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bioRxiv - Molecular Biology 2019Quote: ... The miRNAs were modified by polyadenylation at the 3’ end and then reverse transcribed into the cDNA of miRNA using the miScript PCR Starter Kit (#218193, Qiagen, Valencia, CA, USA) with an oligo dT primer (with a universal tag) ...
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bioRxiv - Developmental Biology 2020Quote: ... rhesus fibroblast cell line (n=1), pri-CTB (n=1, rh090419) and TSCs (n=3 rh121118, rh052318, cy091318) using a FlexiGene DNA Kit (Qiagen, cat no: 51206) and quantified using a Nanodrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
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bioRxiv - Microbiology 2022Quote: ... we added 500 μL of TRIzol and one 3-mm glass bead to the microtubes with mosquitoes and then homogenized the samples in a TissueLyser® (QIAGEN, Hilden, Germany) at 30 Hz for 3 min ...
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bioRxiv - Plant Biology 2021Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen; catalog no. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Microbiology 2022Quote: Total mouse lung RNA was extracted at days 0 and 3 post-challenge using the RNeasy Mini Kit as described above (QIAGEN, Cat. No 74004) and purified with the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was isolated from whole brain tissue from 3 biological replicates per strain for 45 strains of the HRDP using QIAzol (Qiagen, Valencia, CA, USA). The brain was split sagitally and half of the brain was used for RNA sequencing ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant backbone without the Puromycin gene was purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506. A DNA block (Table 3) containing the Blasticidin resistance gene sequence was synthetised (Integrated DNA Technologies ...
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bioRxiv - Bioengineering 2020Quote: ... Approximately 150 mg FW of frozen plant material was disrupted with a glass and a metal bead (Ø 3 mm; QIAGEN GmbH, Hilden, Germany) in a TissueLyser (MM400 ...
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bioRxiv - Genomics 2019Quote: DNA from EndoC-βH1 cells exposed or not to IL-1β and IFN-γ for 48h as described above (3 replicates per condition) was extracted using QIAamp DNA Mini kit (Qiagen, Venlo, The Netherlands). 1 μg DNA aliquots (n=3 ...
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bioRxiv - Plant Biology 2022Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen, Germany; Cat. No. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated microvascular cells were stained for flow cytometric analysis or were additionally processed with myelin removal beads (Miltenyi 130-069-731) and 3 sequential positive selections with CD31 microbeads (Miltenyi 130-097-418) for RNA isolation (Qiagen RNeasy Micro Kit 74004). cDNA library preparation used Oligo-dT at 60 million clusters/sample (University of Chicago Genomics Facility).
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bioRxiv - Cell Biology 2020Quote: ... His6-SUMO-WASHC2C-5-SNAP was purified using lysis buffer #2 and Ni-NTA-agarose beads (Qiagen). The protein was incubated with Ni-NTA beads and washed with 50 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in sphere-forming cultures for 2 weeks prior to RNA collection (RNAeasy Kit, Qiagen). TGFB1 was replenished when placing mOSE cells in sphere-forming conditions.
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bioRxiv - Cell Biology 2019Quote: ... At least 2×102 cells were lysed and RNA was extracted with the RNAeasy Mini Kit (Qiagen) following manufacturer specifications ...
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Microbiology 2022Quote: ... 2×109 bacteria were pelleted and DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen). The optional RNase step in the protocol was performed to remove contaminating RNA ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted twice using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Gene expression was determined by qPCR in a 2-gene custom RT2 Profiler PCR array system (Qiagen) according to manufacturer’s protocol ...