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Citations for Qiagen :
1351 - 1400 of 2030 citations for 1 Bromo 3 difluoromethoxy benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from frozen Hepa 1-6 cell pellets using the RNeasy Mini kit (74106, QIAGEN) following the manufacturer’s instructions with an on-column DNase digestion step (79254 ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with 1 µg of template RNA using the Qiagen QuantiTect reverse transcription kit (Qiagen) in a Bio-Rad T100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was then purified from 500,000 to 1 million cells using the miRNeasy Micro kit (Qiagen GmbH) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a minimum of 1 x106 cells per experimental group were processed using the RNeasy Mini kit (#74104; QIAGEN). The mRNA levels were measured with the NanoDrop (DS-11 ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were washed with 1× PBS and RNA were extracted using the RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then disrupted by sonication and the supernatant was mixed with 1 mL Ni-NTA agarose (QIAGEN) and incubated for 1 h at 4 °C with gentle shaking ...
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bioRxiv - Immunology 2023Quote: ... Total DNA was obtained from the lysed inoculated THP-1 macrophages using DNeasy Blood & Tissue Kit (Qiagen, Germany). The amplification reaction was performed in a 20μl final volume containing 1μl of template DNA ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of culture was withdrawn and chromosomal DNA extracted using the Dneasy blood and tissue kit (Qiagen). The samples were normalized according to DNA concentrations and diluted 1:5 ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μg of RNA per condition was used to retrotranscribe into cDNA QuantiTect® Reverse Transcription Kit (Qiagen). qPCR was performed in 386 wells plates in QuantStudio™ Real-Time PCR System (Applied Biosystems™ ...
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bioRxiv - Biochemistry 2023Quote: ... Negatively supercoiled pHOT-1 DNA was purified from DH5α utilizing a Qiagen Plasmid Midi Kit (Qiagen, Hilden, Germany).
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bioRxiv - Systems Biology 2023Quote: ... Amplicons were gel-purifed from a 1 % Agarose gel using the QIAquick Gel Extraction Kit (QIAGEN, Düren, Germany). After determining the concentration with Qubit (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was carried out using one of the following methods: 1) QIAmp DNA Mini Kit (Qiagen, Germany); 2 ...
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bioRxiv - Microbiology 2023Quote: ... All treatment groups were supplemented with 1 % FCS and RNA was extracted in RNAeasy Plus lysis buffer (QIAGEN) 6 h post treatment.
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bioRxiv - Evolutionary Biology 2023Quote: ... Genomic DNA was isolated from 1 mL of overnight culture of each genotype (DNeasy Blood & Tissue Kit; Qiagen). Paired-end ...
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bioRxiv - Cell Biology 2023Quote: ... cells (1 x 106 cells/well) were washed three times with PBS and lysed in Buffer RLT (Qiagen). Lysates were transferred to Eppendorf tubes on ice and stored at -80°C until ready for RNA purification ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 1 ug of RNA was converted into cDNA using either the Quantitect Reverse Transcription kit (QIAGEN) or the Superscript IV transcription kit (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... V6.5 mESCs and K562 genomic DNA was purified from 1 million cells using the QIAamp micro kit (QIAGEN), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from 1 x 108 trypanosome cells using the Qiagen RNeasy Mini Kit (Qiagen, Netherlands) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then resuspended in 1 mL of lysis buffer from the RNeasy Fibrous Tissue Mini Kit (Qiagen) and processed as per the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: Fragments were purified by adding 300µL phenol:chloroform:isoamyl alcohol (25:24:1 v/v) and sample to a phase lock tube (Qiagen #129046), and spun down at 16,000g for 3 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... or Disp-/- cells were seeded into six-well plates and transfected with 1 µg Shh constructs together with 0.5 µg Scube2 or empty cDNA3.1 by using Polyfect (Qiagen). Where indicated ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of the isolated total RNA was transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). The quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of total DNase-treated RNA using the QuantiTect Reverse Transcription Kit (Qiagen) in 20 µL final volume according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Primers targeting the Mor-1 promotor were designed using the PyroMark Assay Design 2.0 software (Qiagen, Hilden, Germany) and were based on the Ensembl mRatBN7.2 genome build (Table S1) ...
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bioRxiv - Cancer Biology 2024Quote: ... The amplified product was diluted with a ratio of 1 part cDNA to 20 parts EB (Qiagen, 1014609), and concentrations were measured with a dye-fluorescence assay (Quant-iT dsDNA High Sensitivity kit ...
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bioRxiv - Immunology 2024Quote: Total RNA was purified from 0.5-1 million γδ T cells using the RNeasy Plus mini kit (Qiagen) according to the manufacturer’s instructions and the TCR library was prepared using iRepertoire arm-PCR platform-RNA-iR-Complete Dual Index Primer Kit - (iRepertoire) ...
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bioRxiv - Plant Biology 2024Quote: ... TAW1 and its homologues with their annotation IDs are listed in Supplementary Table 1. Amino acid sequences were aligned using CLC Workbench (v. 24.0.1) (Qiagen). Unrooted phylogenetic trees were generated by the neighbour-joining method with 1000 bootstrap replicates in CLC Workbench.
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from approximately 1 million cells using the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) and following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1×106 mNPCs were collected on two separate days and resuspended in 600 µl RLT buffer (#79216, Qiagen). RNA was isolated using RNeasy column purification (#74104 ...
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bioRxiv - Genomics 2024Quote: ... the isolated footprinted DNA (1 µg per sample) was bisulfite converted with the Epitect bisulfite conversion kit (QIAGEN), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... We plotted the change in fluorescence versus temperature (dF/T) using Rotor-Gene Q software (version 2.3.5, build 1; QIAGEN) and compared HRM peaks of amplified samples to bulk genomic DNA and plasmid controls.
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 0.08 µg of pCMVd1-NLS-Gal4-iLight-VP16 (or empty pcDNA3.1+ plasmid) and 0.32 µg of pAAV2-G12-AkaLuc (0.4 µg per well) using Effectene transfection reagent (Qiagen). Culture media was changed 8 h after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA of PDOs grown in ‘Type 1’ culture medium was isolated according to the manufacturer’s protocol using the RNeasy Mini Kit (QIAGEN). Quality and quantity of the RNA samples and the libraries were measured with Agilent’s Bioanalyzer2100 and Invitrogen™ Qubit™ 3.0 Fluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed once with 1 % BSA and incubated with a solution containing 200 μg/ml RNase A (Qiagen) and 50 μg/ml Propodium Iodide (Sigma ...
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bioRxiv - Genetics 2021Quote: ... PCR 2 products were purified by electrophoresis with a 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen) and eluting with 30 μL water ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 μL of primer mix (with a fluorescently labelled forward primer) at 0.2 μM and 1 μL QIAGEN Multiplex PCR Master Mix (QIAGEN). PCR cycling conditions were 95 °C for 10 min followed by 35 cycles of 95 °C for 30 s ...
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bioRxiv - Pathology 2022Quote: ... RNA was isolated from maternal and fetal tissues (Table 1) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Physiology 2022Quote: ... The tissue was homogenized for 1 min at 28.5 Hz in a TissueLyser (Qiagen TissueLyser II, Retsch GmbH, Germany) before samples were rotated end over end for 1 hour at 4°C and centrifuged at 18320g for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were then resuspended in 500 μL of supernatant and stabilized with 1 mL of RNAprotect Bacteria Reagent (Qiagen). The RNeasy® Protect Bacteria Mini Kit (Qiagen ...