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Citations for Qiagen :
1301 - 1350 of 2200 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from 5-10 snap-frozen larvae with the RNeasy Mini kit (Qiagen cat no. 74104) and reverse-transcribed using QuantiTect Reverse Transcription kit (Qiagen cat no 205311 ...
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bioRxiv - Immunology 2022Quote: Total RNA from ~ 5 × 105 neutrophils (CD45+Lin-CD11b+Ly6G+) was isolated with the RNeasy micro kit (Qiagen) and RNA quality was checked with the Agilent 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... Sequences with an E-value lower than 1e-5 were used for multiple sequence alignment using CLC v 21.0.5 sequence manager (Qiagen). After multiple rounds of alignments and manual removing non-nAChR sequences a set of 2047 proteins were obtained ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... High speed supernatant (HSS) was then batch bound to 5 mL of Ni-NTA Agarose (Qiagen, Cat# 30230) resin at 4ºC for 2 hours stirring in a beaker ...
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bioRxiv - Developmental Biology 2023Quote: ... Plates were then placed at 4 0C before adding the reverse transcription mix containing 5’-biotinylated TSO (Qiagen). PCR products were cleaned up using 0.8:1 Ampure XP beads (Beckman Coulter ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Single blastocysts were collected from the M2 drop in 5 µL of 1x TCL lysis buffer (Qiagen #1031586) containing 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... Each of the wells that the nuclei were sorted into contained 5 uL of EB buffer (Qiagen, 19086), 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... with ∼5-10 mg of each organ homogenized into 300 μL homogenization buffer using stainless steel beads (Qiagen) in a TissueLyser II tissue homogenizer (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Total marrow cells were pelleted at 400g for 5 minutes and resuspended and vortexed in RLT buffer (Qiagen) supplemented with 1% beta-mercaptoethanol prior to storage at −80°C ...
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bioRxiv - Cell Biology 2024Quote: ... Pellets were then incubated for 3 h at 37°C with 2ml of 5 mg/ml SDS in 75 mM Tris pH 7.5 with 0.33 mg/ml proteinase K (Qiagen). At this point ...
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bioRxiv - Cell Biology 2020Quote: All muscles were homogenized 2 × 30 sec at 30 Hz using a Tissuelyser II (Qiagen, USA) in ice-cold homogenization buffer (10% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μg total RNA was used for cDNA synthesis using an Omniscript Reverse Transcription Kit (Qiagen). For the quantitative real-time PCR ...
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bioRxiv - Microbiology 2021Quote: ... except that 2 mL prefilled PowerBead tubes (glass beads, 0.1 mm; Cat no. 13118-50, Qiagen) were used for the bead beating ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein purification was performed using Ni+2-NTA agarose affinity chromatography according to standard protocol (Qiagen).
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... counted and 2×104 were lysed in 100 µL of lysis buffer (RLT Plus (Qiagen, 1053393) and 1% ß-mercaptoethanol (Sigma-Aldrich,M3148)
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bioRxiv - Microbiology 2021Quote: ... Tissues were homogenized at 30 Hz for 2 min using TissueLyser II (Qiagen GmbH, Hilden, Germany) and centrifuged for 30 sec at 11000 rpm ...
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bioRxiv - Bioengineering 2020Quote: ... tissue clay and control hydrogels were homogenized for 2 minutes (TissueRuptor) in QIAzol lysis Reagant (Qiagen) and chloroform was added to precipitate and remove proteins ...
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bioRxiv - Bioengineering 2021Quote: ... PCR 2 products were purified by 1% agarose gel using a QIAquick Gel Extraction Kit (Qiagen), eluting with 15 μL of Elution Buffer ...
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bioRxiv - Plant Biology 2021Quote: ... and the resultant supernatant was mixed with 2 ml of Ni-NTA agarose resin suspension (Qiagen) and incubated on a rotary shaker for 60 min at 4°C before transfer to a Poly-Prep Chromatography Column (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen tissue was thawed and immediately homogenized in 2 ml of RTL buffer + 2mM DTT (Qiagen). mRNA was extracted from 300 ul of homogenate using the RNeasy Kit (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was bound to a 2 mL slurry of Strep-Tactin Superflow plus (Qiagen) overnight at 4°C using the batch method ...
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bioRxiv - Physiology 2021Quote: ... Pooled libraries were diluted to 2 nM in EB buffer (Qiagen, Hilden, Germany, Cat. No. 19086) and then denatured using the Illumina protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Tissues were homogenized in a mixture of chloroform and methanol (2:1) using TissueLyser II (Qiagen) and dried in a Vacufuge (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extract was subjected to IMAC by incubation with 2 ml Ni-NTA resin (Qiagen, Germany) per 50 ml of extract ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 15 mg/mL of lysozyme and 2 mg/mL of proteinase K (Qiagen), and incubated at RT for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Each 25 µL qPCR reaction contained 12.5 µL of 2× QuantiTect Probe PCR Master Mix (QIAGEN), 600nM of each primer ...
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bioRxiv - Neuroscience 2022Quote: ... CD11b+ CD45+ cells from one animal (2 retinas) were sorted directly into RLT buffer (QIAGEN 79216) and stored at -80°C ...
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bioRxiv - Genomics 2022Quote: ... Purified PCR products (400 ng) were mixed with 2 μL 10X Taq polymerase PCR buffer (QIAGEN) and nuclease free water to a final volume of 20 μL ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 106 cells were then transfected with 2 μg of DNA using Effecten® transfection reagent (Qiagen) according to the manufacturer’s instructions at a 1:10 DNA/Effecten® ratio ...
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bioRxiv - Immunology 2021Quote: DNA from 1-2 stool pellets was extracted using the QIAamp DNA Stool Mini Kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cultures samples were then mixed 1:2 with RNA Protect Bacteria Reagent (QIAGEN, Germantown, Maryland, USA), vortexed immediately for 5 seconds and incubated for 5 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and gel purified on a 2% agarose gel using Qiagen Gel Purification Kit (Qiagen, Frederick, MD). After quality check using 2100 Bioanalyzer and DNA 1000 chip (Agilent Technologies ...
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bioRxiv - Systems Biology 2022Quote: ... 2 mL cultures were immediately added to centrifuge tubes containing 4 mL RNAprotect Bacteria Reagent (Qiagen), vortexed for 5 seconds and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from the 2 µm filters using the DNeasy PowerWater kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR of cDNA samples was performed using 2× Rotor-Gene SYBR Green PCR Mastermix (Qiagen) and Rotor-Gene Q real-time PCR cycler (Qiagen).
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bioRxiv - Molecular Biology 2020Quote: Cells from 1.0 ml of culture were mixed with 2 ml of RNA protect reagent (Qiagen). The mixture was pelleted at 4000 rpm for 10 minutes and then discard the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was then incubated with 2 ml Ni-NTA Superflow resins (Cat No.30430, QIAGEN) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA was extracted from 2 × 106 cells per duplicate using DNeasy Blood & Tissue Kits (QIAGEN) and sheared to 100-300 bp with a sonicator ...
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bioRxiv - Genomics 2021Quote: cfDNA was extracted from 2-4 mL of plasma using the QIAsymphony liquid handling robot (Qiagen). cfDNA concentration was determined using Qubit double-strand molecular probes kit (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenate was then centrifuged at full speed for 2 min in QIAshredder column (Qiagen, 79656). RNA was extracted from the flow-through using the RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: ... Viral RNA was extracted from VSV-SARS-CoV-2 mutant viruses using RNeasy Mini kit (Qiagen), and S was amplified using OneStep RT-PCR Kit (Qiagen) ...
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bioRxiv - Genomics 2021Quote: ... PCR products were run on a 2 % gel and purified using QIAquick Gel extraction kit (Qiagen).
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bioRxiv - Bioengineering 2021Quote: ... size selected on a 2% agarose gel and purified with a QIAquick Gel Extraction Kit (Qiagen) as per manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... for 1h at 37C and purified from a 2% agarose gel (QIAquick Gel Extraction Kit, Qiagen). In parallel ...
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bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
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bioRxiv - Microbiology 2023Quote: ... each cell cultures (4ml) are treated with 2 volumes of RNA Protect Bacteria reagent (Qiagen, Switzerland) according to the manufacturer’s protocol ...