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Citations for Qiagen :
1251 - 1300 of 1336 citations for Lassa Fever Virus GP1 Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... After incubation microstructures were washed with PBS and RNA was isolated with Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen # 80004). cDNA was synthesized using the SuperScript VILO cDNA synthesis kit (ThermoFisher #11755250) ...
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bioRxiv - Synthetic Biology 2023Quote: ... followed by incubations with a PBS-based protein binding mixture for 1 h and with Alexa488-conjugated anti-His antibody (1:20 dilution, Qiagen 35310) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested 4 days (96hr) after treatment and RNA extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, USA). Concentrations of RNA samples were analysed using Qubit fluorometric assays (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... The induction and purification of the recombinant protein was carried out under native conditions according to the QIAexpressionist manual (“Qiagen”, Germany). For the purpose of fusion protein purification from bacterial cells 1000 ml LB growth medium was inoculated by 25 ml of overnight E ...
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bioRxiv - Bioengineering 2020Quote: ... A Qiagen RT2 SYBR Green master mix with validated qPCR human primers (for HUVECs and BeWo) or mouse primers (for N27 cells) from Qiagen (Frederick) were used to determine relative magnitudes of gene-expression levels using RT-PCR ...
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bioRxiv - Molecular Biology 2021Quote: Total cellular RNA was extracted from mouse placental tissue or HTR cells with an RNeasy Plus Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: LINE1 and SINEB1 probes were similarly generated except using mouse genomic DNA template extracted from E14 mESCs with DNeasy Blood & Tissue Kits (Qiagen 69504) for PCR ...
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bioRxiv - Cell Biology 2021Quote: Whole DNA was extracted from 15-25 mg of mouse tissue using the DNeasy Blood and Tissue Kit (QIAGEN, cat# 69504) according to the kit’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted from mouse retinas using the RNeasy Mini and Micro Kits (Cat No: 74104, 74004, Qiagen, Hilden, Germany). RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: RNA samples from WT and Rlip+/- mouse liver and brain were prepared using the RNeasy mini kit from Qiagen (Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cell lines or kidney and liver of mouse using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Neuroscience 2019Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). Approximately 250 ng of DNA was digested with BAMH1-HF (R3136 ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was isolated from dissected CD31+ BECs from the mouse brain using an RNA extraction kit (#74104, Qiagen, MD, USA). The extracted RNA was stored in RNAse-free water at -80°C until transfer to the Boston University School of Medicine Microarray Core Facility ...
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bioRxiv - Immunology 2020Quote: ... was extracted from two and ten week mouse heart samples stored in RNA Later with the RNeasy fibrous tissue kit (74704, Qiagen, UK). RNA was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: DNA was isolated from P14 tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valencia, CA). For Gabra2 ...
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bioRxiv - Immunology 2022Quote: ... the livers were perfused 45 hr post mosquito-bite challenge with 1× PBS and total RNA was extracted from the lower right caudate process of the caudate lobe of the liver from each mouse using miRNeasy kit (Qiagen: 217004). The complementary DNA (cDNA ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from fibroblasts obtained from each individual mouse from each of the five non-CC inbred strains using AllPrep DNA/RNA Mini kit (Qiagen, #80204). The concentration and purity of extracted DNA was determined using a NanoDrop 2000 UV-vis spectrophotometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... hACE2 expression or SARS-CoV-2 nucleocapsid (N) gene in individual mouse organs was determined using QuantiNova SYBR Green PCR kit (Qiagen #208052) in combination of 500 nM of hACE2 gene specific primer set (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from mouse cortices (~0.25 mg per sample) and infected neuronal cultures (5w2d post transduction) using RNeasy kits (Qiagen, 73304&73404) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from the proteinase K-digested tail tips of mouse or peripheral blood mononuclear cell (PBMC) of human donors using DNeasy blood tissue kit (Qiagen, #69506). 25 ng of genomic DNA was subjected to bisulfite conversion using a commercial kit (Zymo Research ...
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bioRxiv - Biochemistry 2022Quote: ... Memorial Sloan Kettering Cancer Center) at a dilution of 1:5,000) and His (mouse anti-penta-His antibody (Qiagen, cat. #34660) at a dilution of 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from patient tissue samples and flash frozen mouse xenograft tumor samples (n = 4) using the RNeasy kit (Qiagen; #74104) and converted to cDNA using the qScript cDNA Synthesis kit (Quantabio ...
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bioRxiv - Bioengineering 2023Quote: Total RNA from cultured cells and mouse Achilles tendons were isolated with TRIzol reagent and RNeasy Mini Spin Column (Qiagen Sciences) and reversely transcribed into cDNAs using a SuperScript IV VILO Master Mix (Life Technologies).22,30 The relative abundances of genes of interest were determined by TaqMan PCR using primers and probes purchased from Applied Biosystems TaqMan Gene Expression Assays ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was performed using Real-time PCR for RT2 Profile PCR Array Mouse Cytokine and Chemokines using RT2 SYBR® Green Mastermixes (Qiagen). Using the RT2 qPCR Array Data Analysis spreadsheet ...
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bioRxiv - Physiology 2024Quote: 50 mg of mouse spleens were homogenized in 500 μl of the same lysis buffer using the TissueLyser LT (Qiagen, #85600) (50 sec for 2 min ...
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bioRxiv - Neuroscience 2024Quote: ... L4 and L5 DRGs of each mouse were collected and total RNA was extracted using RNeasy Mini Kit (QIAGEN, Cat# 74104). For cDNA synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... approximately 15-20 mg of mouse intestine tissue were used for RNA purification using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: HBEC cells (5e5) from different treatments were mixed mouse RPE1 cells (5e5) for harvesting total RNA using RNeasy kits (Qiagen #74104) for reverse transcription with iScriptTM reverse transcription supermix (Bio-Rad #1708840 ...
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bioRxiv - Cell Biology 2024Quote: ... all samples were diluted to the same RNA concentration and 250 ng of RNA from each sample spiked with 100 ng mouse RNA to control reaction efficiency were reverse transcribed using the miScript II RT kit (Qiagen, 218161) with HiFlex Buffer according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Zoology 2019Quote: ... frozen and stored in liquid nitrogen for extraction of total protein and diene conjugates or placed in QIAzol Lysis Reagent (Qiagen, Hilden, Germany) and frozen in liquid nitrogen for RNA isolation ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell and molecular functions and canonical pathways of these proteins were identified using Ingenuity® Pathway Analysis (IPA®) (Qiagen, Germany).
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bioRxiv - Genetics 2021Quote: ... The final constructs were transformed into BL21 (DE3) for expression and recombinant Fur/mFur proteins were purified using Ni-NTA agarose (Qiagen, California, USA). The proteins induced by the addition of 1 mM IPTG at 16°C for 12 h ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Cell Biology 2023Quote: ... The remaining 80% of the cell suspension was lysed in 6M guanidine-HCl buffer and His6-tagged proteins were captured on Ni2+-NTA resin (Qiagen; cat. #30210). Pull-down eluates and inputs were separated on SDS-PAGE gels and analyzed by immunoblot ...
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bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Membranes were blocked with 5% milk in PBST (Phosphate buffer saline, 0.05% Tween 20) and incubated with monoclonal mouse anti-His antibody (Penta His, Qiagen, dilution 1:1000) according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...