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Citations for Qiagen :
1251 - 1300 of 3750 citations for 6 Chloro 2 3 4 9 tetrahydro 1H pyrido 3 4 b indol 1 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... One microgram of mRNA was converted to cDNA using Quantitect reverse transcriptase (Qiagen), and then diluted 1:10 for subsequent qRT-PCR analysis using SYBR Green JumpStart Taq (Sigma ...
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bioRxiv - Microbiology 2019Quote: One µg of cDNA was synthesised using the QuantiTect reverse transcription kit (Qiagen) with the initial genomic wipe-out step included ...
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bioRxiv - Genomics 2019Quote: ... RNA was extracted from one PBMC sample using the RNeasy mini kit (Qiagen). DNA was extracted from the other PBMC sample using the MagAttract HMW DNA kit (Qiagen).
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bioRxiv - Genomics 2021Quote: ... One million enriched mESCs were lysed and RNA extracted using the RNeasy (QIAGEN) RNA extraction kit ...
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bioRxiv - Neuroscience 2020Quote: ... One metal bead (Peqlab Biotechnologie GmbH, Germany) and 350 μl RLT buffer (Qiagen) including 1% β-mercaptoethanol was added to the frozen tissue parts ...
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bioRxiv - Microbiology 2021Quote: ... the QIAcuity One-Step Viral RT-PCR Kit (Cat No. 1123145, Qiagen, Germany) and the 24-well 26K Nanoplates (Cat No ...
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bioRxiv - Plant Biology 2020Quote: ... Initial amplification was carried out using a One-Step RT-PCR Kit (QIAGEN) in a BIO-RAD T100 Thermal Cycler ...
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bioRxiv - Microbiology 2021Quote: ... The RT-PCR was performed using Qiagen One-Step RT-PCR kit (Qiagen) master mix ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was performed using a One-Step RT-PCR kit (Qiagen, #210210) using the following primers Forward ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram RNA was reverse transcribed using QuantiTect reverse transcription kit (QIAgen, UK). Ten nanograms of diluted cDNA in qPCR water (Roche ...
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bioRxiv - Immunology 2021Quote: One million cells were resuspended in 600 μl of RLT Buffer (Qiagen; 79216) containing 1% 2-BME and snap frozen in a dry ice – ethanol bath for RNA isolation ...
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bioRxiv - Microbiology 2020Quote: ... and RT-PCR carried out using the One-Step RT-PCR kit (Qiagen) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2022Quote: ... Primer extension reaction was performed with one unit of Taq polymerase (QIAGEN, Germany), 0.2 mM dNTP mixture ...
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bioRxiv - Immunology 2022Quote: ... One-step qPCR was done with the QuantiNova SYBR Green qPCR Kit (QIAGEN) by using 20 ng RNA from each donor ...
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA was isolated one day later with Gentra Puregene Cell Kit (Qiagen). Amplicons for long-range sequencing were generated with Q5 High-Fidelity polymerase in 100 uL reactions (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... One µl of cDNA was added to SYBR Green PCR Master Mix (Qiagen) and subjected to PCR amplification (one cycle at 95°C for 20 seconds ...
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bioRxiv - Microbiology 2023Quote: ... containing 500 µl PBS and one 5 mm steel bead (Qiagen, Hilden, Germany) prior to bead-beating for 20 seconds to disrupt the cuticle ...
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bioRxiv - Immunology 2024Quote: ... Digital PCR reactions were run using the QIAcuity One Digital PCR System (Qiagen) in a 96-well 8.5k partitions nanoplate ...
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bioRxiv - Immunology 2023Quote: ... 120 mg l− 1 sodium pyruvate and 1% penicillin–streptomycin) at 300 Hz for 2 minutes using a TissueLyser II (Qiagen, Germany).
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bioRxiv - Genomics 2022Quote: ... 400 nL of protease mix (6 μg protease (Qiagen, 19155), 6.25x NEBuffer 4 (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... For MUS81 interference it was used FlexiTube HsMUS81 6 (Qiagen). SMARCAL1 was depleted using the MISSION esiRNA HUMAN SMARCAL1 (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... 6 million PBMCs were lysed using QIAzol Lysis Reagent (Qiagen). Samples were stored at -80°C until RNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg C19orf43 (purified from E. coli BL21 through Qiagen Ni-TA agarose using standard procedures ...
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bioRxiv - Biochemistry 2023Quote: ... 6 mL of Ni2+-NTA slurry (Qiagen, Venlo, LI, Netherlands) were equilibrated in a gravity flow column with Wash Buffer (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... UniSpike 6 (included in the QIAGEN miRCURY LNA RT Kit) to verify the efficacy of reverse transcription (RT ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted from 1–2 million cells using the AllPrep Mini kit (QIAGEN) according to the manufacturer’s instructions and 1 μg of total RNA was used to prepare each RNA-seq library ...
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bioRxiv - Microbiology 2021Quote: ... and 1% penicillin–streptomycin) at 300 Hz for 2 min using a Tissuelyser II (Qiagen) and centrifuged to clarify the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... and 1% penicillin–streptomycin) at 300 Hz for 2 min using a Tissuelyser II (Qiagen) and were then centrifuged to clarify the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of sample culture was immediately transferred into 2 mL RNA protect bacteria (QIAGEN) and vortexed ...
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bioRxiv - Microbiology 2023Quote: ... we placed filters in 2 mL tubes with beads and 1 mL TE buffer (Qiagen plasmid isolation kit ...
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bioRxiv - Biochemistry 2023Quote: Total RNA was extracted from 1 – 2 x 108 cells using the RNeasy kit (Qiagen). DNA was removed using Turbo DNase (Applichem ...
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bioRxiv - Genetics 2021Quote: ... Electroporated bacteria was split across six tubes and each recovered in 2 mL of SOC for 1 h at 37 °C then added to 500 mL of LB with 100 μg/mL of carbenicillin and grown for 9 h at 37 °C prior to plasmid purification (Qiagen, 12991). The plasmid prep was then normalized to 1 μg/μL to generate the final mpra:minP:gfp library used for transfection delivery.
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bioRxiv - Genomics 2022Quote: ... equal volumes of the clones were combined together and the pool expanded in 20 mL of LB with 100 μg/mL carbenicillin for 9 hr followed by plasmid purification (Qiagen, 12943). Approximately 250 colonies in total were harvested.
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bioRxiv - Biochemistry 2021Quote: Purified CrFBA3 was tested for crystallization at two concentration of 9 mg/ml and 4.5 mg/ml on commercial sparse-screening conditions (Qiagen, Hilden Germany) based on the work of Jancarik and Kim (Jancarik ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 9 AAA and 10 PAD patients’ serum using the miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany), following in principle the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The lysates from the EmulsiFlex B-15 homogenizer were added to Ni-NTA agarose (Qiagen) and incubated on the nutator at 4 °C for 1 h ...
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bioRxiv - Immunology 2020Quote: ... total mRNA was isolated from mouse and human B cells by RNeasy Micro kit (Qiagen), reverse transcribed from mRNA to cDNA for subsequent real-time PCR analysis ...
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bioRxiv - Immunology 2022Quote: Sorted B cells were lysed for RNA extraction by the RNeasy Micro Kit (Qiagen, 74004). Primers used for reverse transcription and library amplification are provided in Table S2 ...
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bioRxiv - Physiology 2022Quote: ... The washed pellet was resuspended in Buffer B with 300 μg/ml DNase I (QIAGEN) and 5 mM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells from the inserts were lysed and collected into RLT buffer containing B-mercaptoethnaol (QIAGEN) for downstream molecular analyses by RT-qPCR.
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bioRxiv - Plant Biology 2024Quote: ... CcAP1 and CcFULLike-A and CcFULLike-B with CLC Genomics Workbench v21.0.3 (QIAGEN, Aarhus, Denmark) (Supplementary Table S5) ...
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bioRxiv - Bioengineering 2024Quote: Sorted B cells were lysed for RNA extraction by the RNeasy Micro Kit (Qiagen, 74004). First-strand cDNA synthesis was performed on 8 μl of total RNA using 5 pmol of IgM and IgG specific primers in a 20 μl total reaction with SuperScript III (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested by the addition of one volume of RNAprotect bacterial reagent (Qiagen) followed by centrifugation at 4000 x g for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... followed by a heteroduplex removal using a one cycle PCR and MinElute Purification (Qiagen) clean up ...
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bioRxiv - Genomics 2020Quote: ... End-point RT-PCR was performed using the one-step RT-PCR kit (QIAGEN) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... One-step qRT-PCR reactions were performed in triplicate using QuantiTech RT mix (QIAGEN) on the Bio-Rad C1000 Thermocycler and CFX96 system (Bio-Rad Laboratories ...
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TrkB induced metastatic potential of cancer by suppression of BMP mediated tumor inhibitory activitybioRxiv - Cancer Biology 2020Quote: ... and RT-PCR analysis was performed using a One-Step RT-PCR kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... one each for RNAseq by freezing in Qiazol (Qiagen, Germantown, MD, USA, Cat #217004), mass spectrometry by freezing in PBS (FisherScientific ...
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bioRxiv - Plant Biology 2020Quote: ... and ground for one minute at 20 m/s in a TissueLyser II (QIAGEN). Then ...
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bioRxiv - Microbiology 2020Quote: ... One half was used for extraction using the DNeasy Blood and Tissue kit (Qiagen) or the GenElute Bacterial DNA extraction kit (#NA2110 ...