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Citations for Qiagen :
1201 - 1250 of 1363 citations for Mouse Anti Hepatitis C Virus NS4b Antibody 1858 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: Total RNA was isolated from mouse liver tissues according to the manufacturer’s instructions (RNeasy® Mini Kit, 74106, Qiagen). RNA samples were incubated with DNase I (79254 ...
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bioRxiv - Systems Biology 2020Quote: ... was prepared by centrifuging a cell culture at 5525xg for 2 minutes at 4°C then resuspending cells in 1mL of PBS-RNAprotect (333 μL RNAprotect Bacteria Reagent [76506, Qiagen, Hilden, Germany], 666 μL PBS). For immediate RNA stabilization by RNAprotect (Fig ...
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bioRxiv - Cell Biology 2020Quote: ... The relative expression of mRNAs of c-Myc regulated genes identified in RT2 Profiler™ PCR Array Human MYC Targets (Qiagen, Cat. no. PAHS-177Z) was determined by real-time quantitative RT-PCR ...
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bioRxiv - Genomics 2022Quote: An aliquot of 150 μL of plasma per sample was thawed on ice and centrifuged at 3000 × g for 5 min at 4 °C and smallRNA was extracted using miRNeasy Serum/Plasma Kits (Qiagen, Cat. No. 217184, Milano, Italy) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from homogenates after overnight incubation with 20 μl of proteinase K at 56°C using the QIAamp DNA Micro Kit (Qiagen, Hilden, Germany, cat. No. 56304) following the manufacturer’s protocol for tissue samples ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The remaining cells were pelleted by centrifugation (4000rpm, 30min, 4C) and stored at –20°C for bulk EEV extraction by endotoxin-free maxiprep (Qiagen EndoFree Maxi Kit, Cat. 12362).
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bioRxiv - Biochemistry 2019Quote: ... proteins were blotted onto PVDF membrane and examined with an RGS-His4-antibody (Qiagen) and a 1:2500 dilution of the fluorophore-conjugated secondary antibody Cy3 (ECL Plex goat-α-mouse IgG-Cy3 ...
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bioRxiv - Microbiology 2021Quote: ... tularensis IM protein SecY (Huntley, 2007) or the Penta-His HRP conjugate antibody (Qiagen).
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bioRxiv - Biochemistry 2021Quote: ... Blots were probed with antibodies against His6 (penta-His Qiagen catalog #34460; 1:10,000), FLAG2 (Sigma catalog #A8592 ...
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bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
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bioRxiv - Physiology 2021Quote: 150-200 islets from each mouse islet preparation were washed with PBS and used to generate RNA samples via the RNeasy Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: DNA for 16S rRNA gene sequencing was extracted from 10-50 mg fecal pellet from each mouse using the DNeasy Powersoil HTP 96 Kit (Qiagen) and an EpMotion 5075 automated pipetting system (Eppendorf) ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from frozen mouse liver (20-25 mg) and HepG2 cells (5-6 million) using the AllPrep DNA/RNA Mini Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse tissue disruption and homogenization was performed in RIPA buffer supplemented with 1:100 protease using Tissue Lyser LT (Qiagen), for 5 min at 50 Hz ...
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bioRxiv - Neuroscience 2020Quote: Total RNA from mouse hippocampi was extracted with the RNeasy mini kit according to the manufacturer’s instructions (Qiagen, Courtaboeuf, France). RNA quality and concentration were assessed by spectrophotometry with the NanodropTM apparatus (Thermoscientific ...
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bioRxiv - Neuroscience 2020Quote: ... including miRNA was isolated from brains of E11 mouse fetuses (3 biological replicates) at 2 h after irradiation using the miRNeasy Mini Kit (Qiagen). RNA was subsequently processed for hybridization to GeneChip miRNA 4.0 microarrays (Affymetrix ...
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bioRxiv - Bioengineering 2020Quote: ... The prepared cDNA was preamplified using the RT2 PreAMP Primer Mix for Human and Mouse PCR Array (Qiagen, PBH-181Z). cDNA was analyzed by RT-qPCR using a Qiagen RT2 profiler custom panel (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... RNA-sea FASTQ data were processed and mapped to the mouse reference genome (GRCm38) using CLC Genomics Workbench 20 (Qiagen). Differential gene expression was performed using the DESeq2 package in R43 ...
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bioRxiv - Bioengineering 2019Quote: RT-PCR was carried out as described previously.27 Total RNA was purified from the mouse retinas using the miRNeasy plus mini kit (Qiagen) and reverse-transcribed with SuperScript III (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... RNA-seq FASTQ data were processed and mapped to the mouse reference genome (GRCm38) using CLC Genomics Workbench 20 (Qiagen). Differential gene expression was performed using the DESeq2 package in R34 ...
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bioRxiv - Microbiology 2022Quote: RNA extracted from mouse lungs as described above was cleaned up using On-Column DNAse using RNAse-Free DNase Set (Qiagen) and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... was used for human ISG15 and ISG56 and mouse genes including Gapdh as the housekeeping gene on the Rotor-Gene Q 5plex (Qiagen). RT-qPCR primers and probes are listed in the supplementary table 2.
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bioRxiv - Bioengineering 2022Quote: ... The mouse genomic DNA was extracted and purified from the whole brain from C57BL/6N mouse by using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2021Quote: ... DRG neurons were transfected in serum free DMEM solution with predesigned fluorescently labelled siRNA directly against mouse TMEM120A (TACAN) from Qiagen-FlexiTube (cat ...
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bioRxiv - Cell Biology 2021Quote: Gene expression studies were conducted on RNA extracted from PTEC cell pellets or from 30-50mg of mouse kidney cortex using the RNAeasy Mini Kit (Qiagen) (see Supplemental Methods) ...
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bioRxiv - Neuroscience 2020Quote: ... Fold enrichment for each primer set was normalized to input and reported relative to a negative control gene desert region (mouse Igx1a (Qiagen). Primers ...
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bioRxiv - Immunology 2021Quote: ... 150 ng DNA per reaction was amplified in duplicate using primers and probes specific to γHV68 Orf50 and mouse Ptger2 (see table) and 2× QuantiNova Probe Mastermix (Qiagen). Standard curves were obtained by serial dilutions of Orf50 and Ptger2 gBlocks (ORF50 ...
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ZMYM2 is essential for methylation of germline genes and active transposons in embryonic developmentbioRxiv - Molecular Biology 2022Quote: ... Isolated genomic DNA (500 ng) of E8.5 mouse embryos (QIAamp DNA mini kit, 51304 or AllPrep DNA/RNA Micro Kit, Qiagen, 82084) and spike-in lambda DNA (1.25 ng ...
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bioRxiv - Systems Biology 2019Quote: ... rRNA depletion of the poly(A)-tailed RNA isolated from yeast cells and mouse tissues was subsequently performed using a GeneRead rRNA Depletion kit (Qiagen), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: ... Briefly, mouse ESCs treated with non-targeting control siRNA (Dharmacon, D-001810-02-50)or NF-YA siRNA (Qiagen, SI01327193) were collected 48 hours post-transfection in cold PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... 4 – 4.5 week-old neurons co-cultured with mouse astrocytes were lysed and total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... were premixed and added to each well of RT² Profiler™ PCR Array Mouse NFκB Signaling Pathway (Qiagen, PAMM-025Z) for target gene qPCR ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative RT-PCR analysis of the samples was carried out using the mouse antiviral response RT2 Profiler PCR array (Qiagen) on a 7900HT Fast Real-Time PCR system (Thermo Fisher ...
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bioRxiv - Cancer Biology 2021Quote: ... gDNA was isolated from at oncogene-negative mouse cell lines or FACS-sorted Tomatopositive cancer cells using either the AllPrep DNA/RNA(Qiagen) or the DNeasy Blood and Tissue Kit ...
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bioRxiv - Neuroscience 2020Quote: ... PCR amplicons of exon 5 of the mouse Nprl3 gene were purified using a PCR purifications Kit (Qiagen, Hilden, Germany) and sent to the Massachusetts General Hospital Center for Computational and Integrative Biology DNA Core for whole amplicon next-generation sequencing (NGS ...
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bioRxiv - Genomics 2021Quote: ... For analysis of Pax8-CreERT2 insert high molecular DNA was prepared from mouse ears using MagAttract HMW DNA Kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted from sorted and unsorted mouse fecal-derived bacterial cells using the AllPrep PowerFecal DNA/RNA kit (Qiagen) as per manufacturer instructions ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: Total RNA from WT and ΔIg3-MuSK mouse soleus and TA was extracted using a RNeasy Fibrous Tissue mini kit (Qiagen) for non-stranded RNA library preparation and sequencing (Genewiz).
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bioRxiv - Immunology 2022Quote: ... Three hundred cells from three to six mouse per condition were sorted directly into 25 μl TCL buffer (Qiagen, 1031576) supplemented with 1%β-mercaptoethanol at single cell precision using a BD FACS Aria II instrument ...
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bioRxiv - Immunology 2022Quote: ... For this analysis bacterial DNA was isolated either from feces or from 1 cm of mouse terminal ileum using QIAmp Fast DNA Stool kit (QIAGEN). qPCR was performed using SYBR Green I qPCR Master mix on LC480II Light cycler (Both Roche) ...
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bioRxiv - Microbiology 2022Quote: Bacterial RNA from mouse GI contents (approximately 250 mg) was extracted using RNeasy® PowerMicrobiome® kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... human THP-1 macrophages (MACs) and acutely isolated mouse microglia was extracted using the RNeasy Plus Mini kit (Qiagen, 74136) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Microglia cultures from mouse pup spinal cords were lysed for total RNA extractions using RNeasy Plus Mini Kit (74134, Qiagen). cDNA generation was performed using High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from the mouse whole hypothalamus and epididymal white adipose tissue were extracted using the RNeasy Universal Plus Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: RNA from human microglia differentiated in vitro and human microglia extracted from human-mouse chimeras was extracted using the RNeasy Plus Micro Kit (Qiagen). The RNA from 3 independent in vitro microglia differentiations per cell line were pooled at equal weight to obtain six samples ...
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bioRxiv - Bioengineering 2022Quote: ... for aliquoting into a 384-well RT2 Profiler PCR Array with genes from the Mouse Inflammatory Response and Autoimmunity set (Qiagen). Plates were analyzed with the Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA of E8.5-E9.5 mouse embryos and HFF-hiPS cells (used as a positive control) were extracted using DNeasy Blood and Tissue Kit (Qiagen) following the manufacturer’s instructions ...