Labshake search
Citations for Qiagen :
1201 - 1250 of 1840 citations for 5 Isoxazolol 4 methyl 3 trifluoromethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: Total RNA was harvested at 4 and 24 hours in biological triplicates using the RNeasy plant mini kit (Qiagen). Purified RNA was verified for intactness on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genomics 2019Quote: ... while the manual plate samples were processed using the Qiagen DNeasy PowerSoil HTP 96 kit (Qiagen, Cat# 12955-4).
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bioRxiv - Microbiology 2019Quote: ... 700 μL of cold methanol and 140 μL of EDTA 0.1M were added and vigorously mixed (4 x 45 s) in a mini-bead-beater-8 (Biospec Products, Qiagen). By this way ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were clarified by centrifugation for 1 h at 4°C at 10,000 rcf before being subjected to Ni-NTA (Qiagen) affinity purification following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... The solution was further cleared at 20,000 × g at 4 °C and the supernatant was incubated with Ni-NTA resin (Qiagen) for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Frozen samples were homogenized with zirconia beads YTZ-4 (AS-ONE, Osaka, Japan) using TissueLyser II (Qiagen, Hilden, Germany), and total RNA was then extracted using the Maxwell 16 LEV Plant RNA Kit (Promega ...
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bioRxiv - Immunology 2020Quote: ... DNA was extracted using the Qiagen MagAttract Power Microbiome kit DNA/RNA kit (Qiagen, catalog no. 27500-4-EP) and used for rRNA sequencing and Helicobacter spp ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from multiples of 4 million resting CD4+ T cells according to the manufacturer’s instructions (QIAamp DNA Mini and Blood Mini Kit, QIAGEN). Amplification of NFL genomes was performed by limiting dilution ...
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bioRxiv - Microbiology 2022Quote: ... The aqueous phase was separated by centrifugation at 13,000 g at 4°C and RNA was purified using the RNeasy Mini Kit (Qiagen) with on-column DNAse digestion ...
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bioRxiv - Immunology 2022Quote: ... Lysate was cleared from debris by centrifugation at 30,000 g and 4°C for 30 min before being loaded onto a Ni-NTA Superflow cartridge (Qiagen) using an Äkta Explorer chromatography system (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... The supernatant was harvested at day 4 after transfection and incubated with Ni-nitrilotriacetic acid (Ni-NTA) agarose (Qiagen). The purification was carried out using gravity flow column and eluted with imidazole-containing buffer as previously described57,58 ...
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bioRxiv - Genomics 2024Quote: ... after the lysis step (step 4 in handbook protocol) and (ii) adding 700 mL of buffer RWT (Qiagen 1067933) instead of the provided RW1 (step 6 in handbook protocol).
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bioRxiv - Microbiology 2024Quote: ... Flag-SHOC2 was cleaved from 6X-His-MBP by incubating 37 mg purified protein with 0.65 mg TEV protease at 4°C overnight followed by affinity purification using Nickel affinity resin (Qiagen) where cleaved Flag-SHOC2 was collected in the flow-through.
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bioRxiv - Microbiology 2024Quote: ... Lysates were clarified by centrifugation at 21,000× g at 4°C and loaded onto gravity flow Ni-NTA agarose columns (Qiagen), followed by washing with 50 mM HEPES pH 7.5 ...
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bioRxiv - Pathology 2023Quote: ... Genomic DNA was extracted from these colonies and from the organs with DNeasy UltraClearn microbial kit (QIAGEN, 10196-4). After performing qPCR ...
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bioRxiv - Microbiology 2023Quote: ... or from perfused organs homogenized (Fisher Bead Mill 4, 2.4 mm metal beads) prior to extraction (DNA Maxi Kit; Qiagen). For STEVOR assay ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2023Quote: ... samples were incubated an additional 30 minutes with 20 uL Proteinase K and 4 uL RNase A (Qiagen 19101) and subsequently eluted for DNA using DNeasy Blood and Tissue kit.
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (3,000 g, 15 min, 4°C) and total RNA was isolated using the miRNeasy Mini Kit (QIAGEN) in combination with the RNase-Free DNase Set (QIAGEN) ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysate was cleared by centrifugation at 100,000 × g for 30 min at 4 ⁰C and then incubated with Ni2+-NTA agarose beads (Qiagen) for 30 min at 4⁰C ...
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bioRxiv - Cell Biology 2023Quote: ... 4°C) and the supernatant incubated 1 hr at 4°C with 1.5 ml packed Ni-NTA Agarose beads (Qiagen). Beads were washed with buffer H adjusted to 50 mM imidazole ...
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bioRxiv - Systems Biology 2023Quote: ... and spleen) (4 samples per treatment and time combination) was isolated with the QIAGEN miRNeasy mini extraction kit (QIAGEN) and cDNA was synthesized with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 2-4 ml of cells were harvested at each timepoint and incubated with 2 volumes of RNAprotect (Qiagen) at room temperature for 5 minutes prior to centrifugation for 5 minutes at 4000xg 4°C ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from pellets of 300 µL culture using a DNeasy UltraClean 96 Microbial Kit (Qiagen 10196-4) or from whole fecal pellets using a DNeasy PowerSoil HTP 96 kit (Qiagen 12955-4) ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from stool swabs stored in RNAlater using the DNeasy PowerSoil HTP 96 Kit (Qiagen 12955-4). To avoid cross-contamination that could lead to erroneous inferences of strain sharing81 ...
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bioRxiv - Microbiology 2023Quote: ... The soluble lysates obtained after two rounds of centrifugation at 18,000g and 4°C were incubated with glutathione agarose beads (Pierce) or Ni2+-NTA beads (QIAGEN). Recombinant proteins were eluted from the beads using 10 mM reduced glutathione in a Tris (50 mM Tris-HCl ...
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bioRxiv - Neuroscience 2024Quote: ... Those included UniSpike 2 and 4 from the QIAGEN RNA Spike-In Kit (Cat. No.: 339390, QIAGEN, Hilden, Germany), which were used to monitor RNA isolation efficacy ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was cleared by centrifugation at 10,000g for 30 min at 4 °C and applied to 15-mL equilibrated Ni-NTA beads (Qiagen). The Ni-NTA column was washed with 150 mL of buffer B (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble lysate obtained after centrifugation at 13,000 rpm for 30 minutes at 4°C was incubated with Ni-NTA agarose resin (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA was then purified by mixing with beads at a 1:0.6 DNA/beads ratio followed by 3 washes with 70% ethanol and eluted with 30 μl of elution buffer (Qiagen Cat# 19086). Whole-exome sequencing was performed using the Mouse_Exome_Targets baitset from the Wellcome Sanger Institute pipeline ...
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bioRxiv - Cell Biology 2020Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts isolated from fresh and cryopreserved tissue from 3 different donors in technical replicates using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... RNA from each Xenopus embryo (n=2-3/condition) was separately extracted and purified using the RNeasy Micro Kit (Qiagen; Venlo, Netherlands) and microarray measurements were performed using the GeneChip Xenopus laevis Genome 2.0 Array (Affymetrix ...
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bioRxiv - Microbiology 2020Quote: ... and each amoeba-adapted and non-adapted population derived from days 3 and 42 using the QIAamp DNA mini kit (Qiagen, Venlo, Netherlands) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from each sample (n=3 WT and n = 3 Fgf9null) using Trizol/chloroform and spin-column assembly with on-column DNase treatment (RNeasy mini kits; Qiagen Germantown, MD). We assessed the quality of RNA using fragment analysis to obtain RNA integrity number (RIN ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from control and treated samples at 3 and 24 hr with three biological replicates (Figure S1) using Qiagen RNeasy Plant Mini kit (Qiagen, Hilden, Germany), with an on-column DNase treatment ...
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bioRxiv - Genomics 2020Quote: ... Sachinoka plantlets grown in vermiculite under 12 h light/12 h dark conditions at 22°C were isolated using the improved 3% CTAB3 method (Yu et al. 2012) followed by treatment with RNase-free DNase (QIAGEN, Venlo, Netherlands) and clean up with RNeasy spin columns (QIAGEN ...
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bioRxiv - Genomics 2020Quote: ... 3 μl of the PCR products was mixed and purified using the QIAquick PCR Purification Kit (Catalog No. 28104, Qiagen, Hilden, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... the pCMV-Neo-Bam p53 R248W vector containing the ampr gene was purified from the transfected SKOV-3 cells using the Qiaprep Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, and PSEN1A246E (replicates, n = 3) human iPSC-derived neurons was prepared using miRNeasy Micro Kit (Qiagen Cat. 217084), based on manufacturer’s procedures ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from magnetically purified NDC, PSEN1M146L, PSEN1A246E, and PSEN1H163R (replicates, n = 3) human iPSC-derived neurons was prepared using RNeasy Plus Micro Kit (Qiagen, Cat. 74034) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 3 ml of each raw milk sample using the PowerFood™ Microbial DNA Isolation Kit (MoBio Laboratories, Qiagen, USA) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: Tocochromanols in 50.3 ± 0.4 mg DW of ground seed powder were extracted in 750 μL of icecold heptane, using two 3-mm diameter glass beads (Carl Roth GmbH+Co, Karlsruhe, Germany) and a Tissue-Lyser (Qiagen, Hilden, Germany) at 25 Hz for 2 min ...
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bioRxiv - Plant Biology 2021Quote: ... 3 leaf discs per sample from different plants were collected in microfuge tubes and ground with a tissue lyser (Qiagen, Düsseldorf, Germany). Serial dilutions were plated on LB agar before counting colonies ...
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bioRxiv - Plant Biology 2022Quote: ... Tissue was ground to a fine powder at −80°C using 3-mm stainless steel beads at 50 Hz for 2*30 seconds in a TissueLyser LT (Qiagen, Germantown, USA). Ground samples were extracted with 1 mL of cold methanol containing [phenyl 13C6]-IAA (0.1 nmol/mL ...
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bioRxiv - Neuroscience 2022Quote: mRNA samples from ANS4-GFP and ANS4-GFP cells co-cultured with bEnd.3 (for 72 hours) were isolated using RNeasy Plus Micro Kit (QIAGEN, cat# 74034). Next-generation whole transcriptome Illumina sequencing (HiSeq4000 ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from 2×105 primary human lung fibroblasts harvested in passage 3 using QIAamp Micro Kit (Qiagen, Hilden, Germany) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Liquid cultures or swabs from agar plates from selected bacterial strains (Supplementary Data 3) were used to extract DNA using the QiAmp Micro DNA kit (Qiagen, Hilden, Germany). The extracted DNA was treated with RNase ...
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bioRxiv - Microbiology 2019Quote: ... and then amplified using 40 cycles of 95°C for 3 s and 60°C for 30 s using either the Rotor-Gene Q or Rotor-Gene 6000 platform (Qiagen, Hilden, Germany).