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Citations for Qiagen :
1151 - 1200 of 1826 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Single cells were index-sorted using a FACS Aria into 96-well plates containing 5μL of lysis buffer (TCL buffer, QIAGEN 1031576) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Neuroscience 2020Quote: ... geniculate ganglion neuron were extracted and transferred to a PCR tube containing lysis buffer as part of the QIAseq FX Single Cell RNA Library Kit (Qiagen). Once in lysis buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The aqueous phase containing the RNA was transferred into a new tube and RNA extraction was performed by using RNeasy 96 kit (Qiagen). DENV titers were determined by quantitative PCR with reverse transcription assay using TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Genetics 2022Quote: ... an amplicon containing a SNP at the Cast allele was amplified by PCR from cDNA using Hot Start Taq (Qiagen) for 38 cycles ...
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bioRxiv - Genomics 2022Quote: ... 32 μl Master Mix containing 30 μl MDA reaction buffer and 2 μl Phi29 polymerase (REPLI-g UltraFast Mini Kit; Qiagen) were added ...
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bioRxiv - Microbiology 2022Quote: ... pH 8.0) containing 1 mg/mL of lysozyme and supplemented with 10 μL of Proteinase K (QIAGEN, cat. no. 19131). The mixture was incubated on a shaker at room temperature for 30 min with intermittent vortexing ...
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bioRxiv - Microbiology 2022Quote: ... at 17,000 G and the pellet containing the microorganisms was used to extract DNA with the DNeasy Blood & Tissue kit as recommended (Qiagen). DNA concentration was estimated by Qubit dsDNA HS kit (Thermo Fisher scientific ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Microbiology 2022Quote: ... Left lungs were harvested in 2 ml Eppendorf (Stevenage, UK) Safe-Lock micro test tubes containing 500 µl DMEM and a stainless-steel carbide bead (Qiagen), and snap frozen on dry ice until virus titration ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR product containing gfp-lcn was recovered from the agarose gel and purified using the gel extraction kit (Qiagen). The purified PCR product was digested with HindIII and BamHI and purified by the PCR purification kit (Qiagen) ...
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bioRxiv - Systems Biology 2022Quote: ... The digested DNA fragment containing the tapA promoter was recovered from the agarose gel and purified using the gel extraction kit (Qiagen). The GFP coding region was amplified by PCR with primers omf316 and om528 using pMF719 (thrC: ...
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bioRxiv - Pathology 2023Quote: Total RNA from HDLEC treated or not with conditioned media containing APLN were harvested 24h after treatment and isolated using the RNeasy mini kit (Qiagen). DNA digestion was performed using the RNase-Free DNase set (Qiagen) ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 100,000 cells were transferred to a tube containing 100 µl of RLT buffer (Qiagen, Hilden, Germany). Cells were stored at –80 °C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted into 96-well plates containing lysis buffer with proteinase K solution in PKD buffer (1:16) (Qiagen) and were stored at -80ºC ...
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bioRxiv - Biophysics 2023Quote: ... beads were discarded and the supernatant containing reconstituted into ND A2AAR was incubated for 4 h with 250 µL of Ni-NTA resin (Qiagen) for separating from empty ND ...
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50 μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Microbiology 2023Quote: ... a single GFP+ or GFP-J-Lat 11.1 cell was sorted directly into each well of a 96-well PCR plate containing the pre-amplification mastermix consisting of 1 μL One-step RT-PCR enzyme (QIAGEN), 5 μL 5× One-step RT-PCR buffer (QIAGEN) ...
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bioRxiv - Neuroscience 2023Quote: ... The aqueous phase containing RNA was transferred to a new tube for extraction and clean-up using the RNeasy Mini Kit (QIAGEN) with an on-column DNase digestion as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The cleared lysate was applied to an affinity chromatog-raphy column containing Ni-nitrilotriacetic acid (NTA) su-perflow resin (Qiagen) pre-equilibrated in lysis buffer ...
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bioRxiv - Genomics 2022Quote: ... We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50, MoBio – now QIAGEN PowerBead) using sterile tweezers ...
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bioRxiv - Genomics 2023Quote: ... The BD FACS Melody sorter device was used for sorting single cells into 96-well plates (FrameStar®, 4TI-0960/C) containing 2.5 μl RLT plus buffer (Qiagen). Plates were then spun down at 1,000g for 1 minute at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... calf muscle and pancreas were collected in 500 μl of PBS containing one (heart and pancreas) or two (calf muscle) 5 mm stainless steel beads (QIAGEN), homogenized with TissueLyser II (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... containing RNA derived from the entire cortical sample was collected and lysed with 350 µl of buffer RLT from Qiagen RNeasy Kit supplemented with 10μl/ml bME ...
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bioRxiv - Neuroscience 2023Quote: ... brain hemispheres were homogenized in freshly prepared lysis buffer (0.6 ml per 30 mg of brain tissue) containing 1% β-mercaptoethanol using a TissueRuptor homogenizer (Qiagen).
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bioRxiv - Immunology 2023Quote: ... The first round of PCR was carried out using the ImmunoSEQ proprietary PCR primer mix (32 μL per sample containing 25 uL of QIAGEN 2× Multiplex PCR Master Mix ...
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50 μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Neuroscience 2023Quote: ... the upper aqueous phase containing RNA was mixed with equal amounts of 100% ethanol and was then loaded onto an RNA spin column (Qiagen) and centrifuged for 30 seconds at 8000 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... and total RNAs containing SINEUP-GFP RNAs at the aqueous phase were extracted by using an RNeasy mini Kit (Qiagen). The RNA was then digested with 4 U of TURBO DNase (TURBO DNA-free kit ...
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bioRxiv - Microbiology 2023Quote: ... Triplicate PCRs were carried out in 15 µl reactions containing 1x UCP Multiplex PCR Master Mix (Qiagen, Venlo, The Netherlands), 0.3 μmol l−1 each of the forward and reverse primers ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted directly into 2-Mercaptoethanol-containing RLT buffer and RNA was extracted using a RNeasy Mini kit (Qiagen). The cell populations used for RNA sequencing are summarized in Table S1 ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Oligonucleotides were incubated with a PCR mix containing (100 µM dNTP, 1X thermopol Taq reaction buffer, 1X of Q solution (Qiagen) and 1 µM of each primer) ...
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bioRxiv - Microbiology 2023Quote: ... in vitro in L-lactate containing minimal media and total RNA was isolated by the TRIzol method and RNeasy minikit (Qiagen). After digestion with TURBO Dnase treatment ...
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bioRxiv - Synthetic Biology 2023Quote: Bacterial clones on selection plates were scraped and pooled to extract recombinant plasmids containing the recipient barcodes and DNA blocks (donor barcodes, oligonucleotides, and E. coli ORFs) using Plasmid Plus Mini Kit (QIAGEN). The pooling capacity is determined by the number of unique positioning barcodes ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted from 1/5th of the total volume of input WCEs and from 300 µl of each set of pooled fractions containing 80S or different polysomal species using QIAzol lysis Reagent (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... After chloroform incubation and centrifugation per manufacture instructions RNA-containing phase was diluted with 70% ethanol 1:1 and RNA isolation was performed using RNeasy Mini Kit (Qiagen). For tissues ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was isolated from spleens of reconstituted lymphoma bearing mice (containing malignant and normal hematopoietic cells) using DNeasy Blood and Tissue Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Two thousand to eight thousand HSCs or MPPs were sorted into tubes containing RLT buffer from the RNeasy Micro Kit (Qiagen). Total RNA was extracted according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The bits containing the powder was immediately transferred and rinsed in a 2 ml Eppendorf (DNA/RNA and DNase/RNase free tube) containing 1 ml of Tissue Lysis buffer ATL from the QIAamp DNA Micro Kit (Qiagen) and stored at 4°C until the DNA extraction process (i.e ...
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bioRxiv - Genetics 2024Quote: ... they were transferred to Eppendorf tubes containing either 180 µL buffer ATL and 20 µL Proteinase K (Qiagen, Hilden, Germany), or 350 µL of in-house ZB5d medium/antibiotics mixture as described in Ries et al ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria are harvested by centrifugation (5 minutes, 4°C) and washed with 1 ml cold PBS containing RNA stabilization reagents (RNAprotect, Qiagen) before flash freezing and storage at -80°C ...
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bioRxiv - Cell Biology 2024Quote: ... genomic DNA was extracted from cell pellets containing at least 1e6 cells/pellet using the DNeasy Blood and tissue kit (Qiagen), followed by bisulfite conversion using the Epitech Fast Bisulfite Conversion Kit (Qiagen) ...
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bioRxiv - Plant Biology 2024Quote: ... The fresh leaf discs were transferred to a 2 mL tube containing a 5 mm stainless steel bead and 500 µL buffer RLT (QIAGEN). The leaf discs were disrupted using a TissueLyser LT (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from MSCs cultured for 3 or 15 days under different experimental conditions using RNeasy Micro Kit (Qiagen). RNA quantity and integrity was measured with a NanoDrop 1000 (ThermoScientific) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from heparinised blood of SI and SH mice (n=3 per housing group) using the RNeasy Protect Animal Blood Kit (Qiagen). Extracted RNA was hybridised at UCL genomics following standard Affymetrix protocols ...
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bioRxiv - Genetics 2021Quote: ... and E11 (5’-AGGAAAAAGGAAATAAATTA-3’) primers on pDH373 as a template generated a smaller fragment that was cleaned up by QIAGEN MinElute PCR Purification Kit (#28004) ...
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bioRxiv - Developmental Biology 2021Quote: ... E18.5 RNA was extracted from cortices of 3 genotypic conditional knockouts and 4 controls using the RNeasy Micro Kit (Qiagen). RNA was reverse transcribed to cDNA using the SuperScript™ II Reverse Transcriptase Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... LRRCC1-si2 (target sequence: 5’- TTA GAT GAC CAA ATT CTA CAA - 3’) and control siRNA (AllStars Negative Control) were purchased from Qiagen. siRNAs were delivered into cells using Lipofectamine RNAiMAX diluted in OptiMEM medium (ThermoFisher Scientific) ...