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Citations for Qiagen :
1151 - 1200 of 2259 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: Approximately 3-4 million cells were solubilized with 1 mL QIAzol (Qiagen, 79306) and 0.2 mL chloroform in 5PRIME Phase-Lock Gel heavy tubes (QuantaBio ...
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bioRxiv - Genetics 2024Quote: ... the cells were lysed in 1 mL of RLT buffer obtained from Qiagen, and for Mass spectrometry ...
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bioRxiv - Molecular Biology 2024Quote: ... castellanii cells at a concentration of 1 μg using Effectene transfection reagent (QIAGEN). Cells were infected with Mimivirus (MOI-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 μg plasmid DNA (1 μg/μL; prepared by QIAGEN Plasmid Midi Kit) in 30 μL MMG solution was used to transform 200 μL protoplasts by gently mixing with 230μL 40% PEG transformation buffer ...
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Reduction of chromosomal instability and inflammation is a common aspect of adaptation to aneuploidybioRxiv - Cell Biology 2023Quote: RPE-1 p53KD cells (parental and clones) were harvested in buffer RLT (Qiagen). Strand-specific libraries were generated using the TruSeq PolyA Stranded mRNA sample preparation kit (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... 1 h 45 min] and resuspended in 360 µL of buffer ATL (Qiagen). DNA was extracted in duplicate with the QIAamp DNA Mini Kit (Qiagen ...
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bioRxiv - Neuroscience 2022Quote: ... 1% of input was saved in RLTplus buffer (Qiagen RNeasy Micro Kit #74034) supplemented with 2-Mercaptoethanol before 20µl or 15µl of HA-magnetic beads (Pierce ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was incubated with 1 mL of Ni-NTA beads (Qiagen, USA) while rotating for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The worms were then suspended in 1 ml RLT buffer (Qiagen RNeasy kit) containing β-mercaptoethanol and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was added to a 1-mL Ni-NTA agarose column (QIAGEN). The column-bound HigB2–HigA2 complex was disrupted by washing the column with buffer containing 5 M guanidine-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... + 1% β-mercapto-ethanol and RNA was purified using the RNeasy kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... This was then added to 1 ml of Ni-NTA Agarose (Qiagen, 30310) and left on a rolling platform at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was incubated for 1 hr with Ni-NTA agarose beads (Qiagen) equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... using specific primers (Supplemental Table 1) in a Rotor-Gene Q machine (Qiagen). Standard curves were achieved by dilutions of one cDNA sample ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with 1 ml of Ni-NTA agarose beads (Qiagen), equilibrated in the lysis buffer for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a 1 ml Ni-NTA Superflow cartridge (QIAGEN). The column was washed with 10 column volumes (CVs ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA from 1×106 cell was first extracted with RNeasy Plus kit (Qiagen), per manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... RNA (1 μg) was reverse transcribed using the QuantiTect Reverse Transcription Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... with zirconia/silica beads in 1 mL RLT buffer (Qiagen, Germantown, MD, USA). RNA was extracted using the Zymo Direct-zol RNA Miniprep Plus kit as recommended by the manufacturer ...
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bioRxiv - Immunology 2024Quote: A total of 1 x 106 cells were lysed using RLT buffer (QIAGEN) and total RNA was extracted using RNeasy isolation kit plus (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... lymphophilum API-1 were extracted using the QIAamp PowerFecal Pro DNA kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of purified RNA was reverse transcribed using RT2 First Strand Kit (Qiagen) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: NHEKs were transfected at 50-70 % confluency with 1 µL HiPerfect transfection reagent (Qiagen) and 5 nM of either AhR-specific “SilencerSelect” siRNA (s1199 ...
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated from 1×107 cells with the RNeasy® Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: RNA was extracted from MDM or THP-1 cells using RNAeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The qPCR mix (10 μL) contained 1× Rotor-Gene SYBR green PCR mix (Qiagen), 500 nM each primer ...
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bioRxiv - Immunology 2021Quote: ... Periplasmic fraction was incubated with 1 mL pre-washed Ni-NTA agarose (30230, Qiagen) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA was extracted using the RNeasy kit and treated with DNase 1(Qiagen). Three donor samples of human RPE/choroid were processed for RNAseq ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA was extracted from 1-2 million cells using RNeasy Mini Kit (QIAGEN) using QIAcube (QIAGEN) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µL of bisulfite-converted DNA was amplified with HotStar Taq DNA polymerase (QIAGEN) in a 25 µL reaction using the primers designed with MethPrimer [107] and listed in Supplementary Table S2 ...
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bioRxiv - Microbiology 2019Quote: Total DNA was isolated from 1×108 cells using Puregene Core Kit A (Qiagen). Free minicircles and total kDNA content were analyzed as previously described (Bruhn ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h at 37 °C and purified using QIAquick PCR Purification Kit (Qiagen). The pLVX-Puro lentiviral expression vector (Clontech ...
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bioRxiv - Genomics 2021Quote: ... DNA was extracted from 1 million mESCs using DNeasy Blood & Tissue Kit (Qiagen, 69506) and RNA was extracted from 450,000 HEK293T cells using the Direct-zol RNA MicroPrep Kit (Zymo ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 ml of the slurry using a commercial kit (Qiagen).
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bioRxiv - Neuroscience 2020Quote: ... or its mutants (200 ng) and GCaMP6 (1 μg) using the Effectene reagent (Qiagen). The human orthologue of the mouse splice variant TRPM3α2 in the pCDNA3.1(+ ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of RNA was retrotranscribed in cDNA with QuantiTect Reverse Transcription Kit (Qiagen), followed by a PCR amplification of the subsequent transcripts ...
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bioRxiv - Cell Biology 2021Quote: ... The crude PCR product was gel purified from 1% agarose by spin column (Qiagen), and 200 ng of the purified amplicon were end-labeled using 10 µCi of γ-32P ATP (Amersham ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg RNA using a Quantitect Reverse Transcription kit (Qiagen) and diluted 1:20 in DEPC-treated water ...
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bioRxiv - Plant Biology 2021Quote: ... swc6-1 and pfd3,5 seedlings (three biological replicates) using RNeasy Plant Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed with Quantitect Reverse Transcription Kit (Qiagen) using oligo(dT) ...
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bioRxiv - Microbiology 2020Quote: ... served as hosts for derivatives of plasmids pGEX-6P-1 (Cytiva) and pQE30 (Qiagen), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Each PCR contained 25 µL of 1 × Taq PCR Mastermix (Qiagen GmbH, Hilden, Germany), final concentration of 0.2 µM of each primer ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of template DNA and the Qiagen PCR multiplex PCR kit (Qiagen, France) on a T100TM Thermal cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: rhGAA secreted from D532 was purified using 1 mL Ni-NTA agarose resin (Qiagen) according to the manufacturer’s instructions and as described previously [27] with slight modification ...
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bioRxiv - Genomics 2022Quote: ... The product was purified from a 1% agarose gel (QIAquick Gel Extraction Kit, Qiagen).
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was supplemented with 1 mL pre-equilibrated Ni-NTA resin (Qiagen) and 10 mM imidazole and stirred for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and homogenized for 1 cycle with a Bead Beater TissueLyser II (QIAGEN, Germantown, MD) at 25 Hz for 1.5 min ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was added to 1 mL of washed Ni-NTA bead slurry (Qiagen) and incubated for 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Cells were sorted into PBS/1% FBS before resuspension in Buffer RLT (QIAGEN, USA) or directly into Buffer RLT with 1% 2-β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... The Ni-NTA matrix was loaded on a prepared 1 ml Polypropylene column (Qiagen) and a filtered P ...