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Citations for Qiagen :
1151 - 1200 of 2270 citations for 2 Chloro 4 3 fluoro 4 hydroxyphenyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Samples were incubated for 3 minutes at 56°C in a deparaffinization solution (Qiagen, 19093). A step of digestion with Proteinase K was performed followed by a treatment with DNAses ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was then incubated with 3 ml of Ni-NTA Superflow agarose beads (QIAGEN), which were preequilibrated with bacterial lysis buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Leaf samples were collected in the well containing a 3 mm tungsten carbide bead (Qiagen) and 100 µl extraction buffer (200 mM Tris-HCl ...
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bioRxiv - Physiology 2024Quote: ... 3 mM benzamidine) with a steel bead7,17 using the TissueLyser II bead mill (Qiagen, USA) for 1 min at 30 Hz.
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bioRxiv - Pathology 2024Quote: ... the stock 3’DIG-labelled probes (scrambled (included in the miRCURY LNA Control Set - Qiagen); miRNA-34a custom probe YD00618250-BEG ...
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bioRxiv - Cancer Biology 2024Quote: RNA library construction was conducted by using QIAseq UPX 3’ transcriptome kit (Qiagen, catalog# 333088). 7ng purified RNA for each sample (n = 4 per group ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acids (DNA/RNA) were isolated from flash-frozen tissues using the All Prep DNA/RNA Mini Kit (Qiagen, Germantown, MD) as previously described [127–129] ...
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bioRxiv - Neuroscience 2021Quote: ... double-digoxigenin locked nucleic acid probe (RNO-MIR-124-3P: CATTCACCGCGTGCCTTA, Tm: 84°C, 339111 YD00614870-BGC, miRCURY LNA™ miRNA Detection Probe, Qiagen) was used at a final concentration of 30 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed twice in PBS and incubated for 20 min at 54 °C (60 min at 52 °C for tissue sections) in mercury locked-nucleid acid (LNA) miRNA ISH Buffer 80 nM hybridiziation buffer (100 nM for tissue section) (Qiagen, 339450). Digoxigenin labeled miRCURY LNA probes (HSA-MIR-10B-5P ...
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bioRxiv - Microbiology 2021Quote: Samples were homogenized and nucleic acids were extracted using the MoBio RNA PowerSoil® Total RNA Isolation Kit (Qiagen, CA, USA) and the MoBio RNA PowerSoil® DNA Elution Accessory Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: Frozen tissues were weighed and homogenized in RLT and nucleic acids were extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN, #80204) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated using the RecoverAll Total Nucleic Acid Isolation Kit (Thermo #AM1975) and concentrated using the RNeasy MinElute Cleanup Kit (Qiagen #74204). Library preparation and sequencing were performed by the Genome Sequencing Facility at St ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was divided into two parts: one part was directly combined with nickel-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen, China); the other part was heated in a 65 °C water bath for 60 minutes and then centrifuged at 14000 rpm for 60 minutes ...
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bioRxiv - Immunology 2021Quote: ... The plasmid was transfected to 293T cells and the recombinant S protein trimers were purified by Ni-NTA (nickel-nitrilotriacetic acid) chromatography (QIAGEN, Germany), followed by size exclusion to further purify the trimers ...
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bioRxiv - Microbiology 2022Quote: ... nucleic acid extractions were performed by combining equal amounts of cell culture supernatants with RLT Lysis Buffer (Qiagen, Germantown, MA, USA), with 200 µL of the lysate used for magnetic bead-based extraction according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was adjusted to 500 mM NaCl and 20 mM imidazole before affinity purification by incubation with nickel-nitrilotriacetic acid (Ni-NTA) resin (Qiagen, 30210) for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Lysates of His-tagged α-catenin WT and Δmod were bound to Ni-NTA (Ni2+-nitrilotriacetic acid)-sepharose affinity chromatography (Qiagen), washed with 50 mM Tris pH 7.8 ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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First complete genome characterization of an Indian pigeon pox virus directly from a clinical samplebioRxiv - Evolutionary Biology 2023Quote: ... The extraction of nucleic acid from infected scab samples was performed by using DNeasy blood and tissue purification Kits (QIAGEN, USA) with some modification established by Sarker et al. ...
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bioRxiv - Neuroscience 2024Quote: Ribonucleic acid (RNA) was purified from the dissected ventral mesencephalon containing SNpr using RNeasy Microarray Tissue Kit (Qiagen, Copenhagen, Denmark, DK) with an on-column deoxyribonuclease (DNase ...
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bioRxiv - Cancer Biology 2024Quote: ... additional DNA from 1-5 mL of plasma was extracted using the QIAamp® Circulating Nucleic Acid Kit (Qiagen, catalogue # 55114). Illumina sequencing libraries were constructed using SRSLY® PicoPlus DNA NGS Library Preparation Kit (ClaretBio – Cat ...
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bioRxiv - Biochemistry 2024Quote: ... the EcNhaA triple mutant was extracted from membranes with n-Dodecyl β-D-maltoside (DDM; Glycon) and purified by Ni-nitrilotriacetic acid (Ni-NTA; Qiagen) affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... cells in each well were transfected with 50 μM of each ASO locked nucleic acid GapmeR1 or GapmeR2 (Qiagen, custom design) with Negative Control GapmeR A (30300019-2 ...
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bioRxiv - Microbiology 2024Quote: ... Viral nucleic acids were then extracted from swab filtrates and tissue homogenate supernatants using the QIAamp Viral RNA Mini Kit (Qiagen, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... Purification was carried out by Nickel affinity chromatography using a Ni-NTA resin (Ni2+-nitrilotriacetic acid resin, Qiagen, Valencia, CA, USA) following the manufacturer’s recommendations under denaturing conditions.
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bioRxiv - Developmental Biology 2024Quote: ... Cell pellets were immediately used for insulin isolation using the acid ethanol extraction method 52 and genomic DNA isolation using the DNeasy Blood & Tissue kit (Qiagen, 69504). The human C-peptide enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Genomics 2021Quote: ... and Vero E6 cells infected with 2 patient virus isolates was converted to cDNA using reverse transcriptase from qiaseq SARS-CoV-2 primer pool (Qiagen kit, cat no. 333896). APOBEC3b (sense ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2 min at 40 Hz using TissueLyser LT (Qiagen). 500 µl PBS-tween (0.01% ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL of nickel-charged resin (Qiagen, Ni-NTA Agarose) was added to a column (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... using the QIAstat-Dx Respiratory SARS-CoV-2 Panel (Qiagen). Viral stocks were produced infecting at a multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2022Quote: ... in a 2 mL cryovial using a tissue lyser (Qiagen) and 0.2mm ceramic beads ...
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bioRxiv - Microbiology 2021Quote: ... or using the QIAseq SARS-CoV-2 Primer Panel (Qiagen) (for organoid supernatants) ...
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bioRxiv - Cell Biology 2022Quote: ... with 1% 2-mercaptoethanol then passed through Qiashredder tubes (Qiagen). RNA was extracted using the RNeasy Isolation Kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... QIAseq SARS-CoV-2 Primer Panel (Qiagen, cat. no. 333895) and QIAseq FX DNA Library Kit were used in order to prepare amplicon libraries for viral genome sequencing ...
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bioRxiv - Immunology 2020Quote: ... and extension by 2 × multiplex PCR master mix (QIAGEN, USA) at 72 °C for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... loaded with 1-2 ml Ni-NTA agarose resin (Qiagen) pre-equilibrated with binding buffer ...
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bioRxiv - Genomics 2022Quote: ... and ‘Sy-2’ plants using Genomic Tip (Qiagen, Hilden, Germany), and high-molecular-weight DNA (fragment length > 40 kb ...
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bioRxiv - Neuroscience 2022Quote: ... and IRS solution (Qiagen Cat No./ID: 26000-50-2) for a custom protocol ...
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bioRxiv - Microbiology 2022Quote: ... for 2×2min at 30Hz in a TissueLyser II (Qiagen). After homogenization ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Pellets were thawed in 2 mL RLT lysis buffer (Qiagen) containing 10 μL mL-1 of 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips were treated with 2 mg/ml RNase A (QIAGEN) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... 2 μL of HiPerfect transfection reagent (cat. no. 301705, Qiagen) were mixed with 100 μL NeuroBasal medium without supplements ...
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bioRxiv - Developmental Biology 2023Quote: ... and RNU6-2 (Hs_RNU6-2_11 miScript Primer Assay, Qiagen, MS00033740) as housekeeping miRNA.
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bioRxiv - Biochemistry 2023Quote: ... then loaded on to 2 mL Ni-NTA column (Qiagen) pre-equilibrated with 10 column volumes of 50 mM Tris pH 7.5 containing 300 mM NaCl ...
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bioRxiv - Genomics 2023Quote: ... using a TissueLyzer II (QIAGEN, 2min, 25 Hz, 2 times). The samples were incubated at room temperature for 5min ...
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bioRxiv - Immunology 2023Quote: ... following a 2-step VDJ amplification using HotStarTaq Plus (Qiagen). PCR products were enzymatically cleaned again and illumina adapters and indexes were introduced via PCR ...
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bioRxiv - Cell Biology 2023Quote: ... equipped with a 48-sample holder (Tissue Lyser 2, Qiagen). The samples were treated with 1 ml pre-cooled (-20°C ...
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bioRxiv - Microbiology 2023Quote: ... fitted with a 2 ml tube holder (Qiagen, Germantown, MD). RNA was purified with the ZymoBIOMICS RNA Miniprep kit (R2001 ...
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bioRxiv - Immunology 2024Quote: ... 2 million PBMC were lysed using QIAzol Lysis Reagent (Qiagen). Samples were stored at -80°C until RNA extraction ...
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bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...