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Citations for Qiagen :
1101 - 1150 of 1827 citations for Methionine aminopeptidase 1 METAP1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... The clear supernatant was then gently mixed with 1 ml of Ni+2-NTA agarose beads (Qiagen, Germany) for 30 min and then added to the column with a flow rate of 0.5 ml/min ...
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bioRxiv - Genetics 2019Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2019Quote: ... 1 million cultured 12878 cells using the Gentra Puregene Blood Kit following manufacturer’s instructions (Qiagen, Cat. No 158467). To generate longer DNA fragments (WμFL=150kb and longer ...
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bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
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bioRxiv - Cell Biology 2020Quote: ... collected in 1 mM EDTA in PBS and total RNAs were isolated using RNeasy Mini Kit (Qiagen, 74106) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... 120 µL of elution buffer of elution buffer II (10 mM Tris pH 8.0, 1 mM EDTA, 0.67% SDS) and 7 µL Proteinase K (Qiagen) was added ...
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bioRxiv - Microbiology 2021Quote: ... prior to the addition of proteinase K and 5 μl of 100 mg ml−1 RNase A (Qiagen). The DNA concentration was determined using a Qubit fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... We then washed beads four times in 1 ml of Modified RLT buffer (RLT buffer supplied by Qiagen with added 10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2021Quote: Up to 1×106 culture cells were collected in 350 μl of RLT buffer (QIAGEN RNeasy Mini Kit). RNA was extracted following the QIAGEN RNeasy manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of total RNA was subjected to cDNA synthesis using the QuantiTect Reverse Transcription Kit (Qiagen, 205314). Quantitative real-time PCR reactions were performed with the SYBR Select Master Mix (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... at 37°C for 1 hour and cleaned up with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to manufacturer instructions ...
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bioRxiv - Clinical Trials 2019Quote: Genomic DNA from 1-10 Million CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen). In some experiments ...
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bioRxiv - Developmental Biology 2020Quote: ... see Supplemental Table 1 for more details) using either: PN3 ovaries - QIAzol Lysis Reagent (Qiagen, Chadstone, VIC, Australia) and Glycoblue coprecipitant (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Membranes were washed with PBS-T (PBS1x/0.1% Tween 20) and incubated with mouse antibody against 6xHis-tag (1:2,000; Qiagen), rabbit antibody against GST-tag (1:10,000 ...
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bioRxiv - Cell Biology 2019Quote: ... Chambers were washed with BRB80 and then were coated with mouse antibody against 6xHis-tag (1:50; Qiagen) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR amplifications were carried out using Power SYBR Green RNA-to-CT 1 step kit (Qiagen, UK) on a 384 well ViiA Real-Time PCR System (Applied Bio-systems ...
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bioRxiv - Biochemistry 2021Quote: Total RNAs were extracted from wild-type or DACR HAP-1 with the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of TIE-1 was isolated using the DNeasy Blood and Tissue kit (Qiagen, Valencia, CA, USA) and used as a template for PCR reactions ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was prepared from 1 μg of total RNA using the QuantiTect RT Kit (Qiagen, Venlo, the Netherlands). Quantitative RT-PCR was performed using TB Green Premix ExTaq II (Takarabio ...
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bioRxiv - Genomics 2021Quote: ... The reaction was stopped by adding 1 μL 0.5 M EDTA (pH = 8.0) then cleaned up with MinElute PCR Purification Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2022Quote: ... mycelia were harvested by filtering and immediately homogenized for 1 min at 30 Hz using a TissueLyzer (Qiagen). Then ...
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bioRxiv - Microbiology 2022Quote: ... and sgRNA-specific and universal primers (Table 1) and subsequently purified with the QIAquick PCR Purification Kit (Qiagen). sgRNAs mRNA was transcribed using AmpliScribe-T7 Flash Transcription kit (Epicentre) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from 1 ml of cells from each culture using a miRNeasy kit (Qiagen, Germany) with some modifications ...
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bioRxiv - Systems Biology 2022Quote: ... siRNA was transfected into RWPE-1 cells in a 24-well plate using HiPerFect Transfection Reagent (Qiagen, 301704). siRNA ...
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bioRxiv - Microbiology 2022Quote: Total community DNA was extracted from 1 g of crushed rocks using DNeasy PowerSoil Pro Kit (Qiagen, German), quality checked by electrophoresis using a 1.5% agarose gel and Nanodrop spectrophotometer (Thermofisher ...
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bioRxiv - Molecular Biology 2022Quote: Whole RNA was extracted from over 1 × 10^5 cells using the QIAGEN RNeasy Micro kit (QIAGEN, 74004) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... diluted in Milli-Q H2O 1:2 and the cells disrupted by mechanical lysis in the TissueLyser (Qiagen) for 2 minutes at 30 Hz and three freeze/thaw cycles (20° to -20°C).
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription reactions were performed with 1 μg of total RNA with the QuantiTect Reverse Transcription kit (Qiagen). Gene expression was measured using TaqMan™ master mix (Thermofisher ...
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bioRxiv - Physiology 2024Quote: ... using 350 microliter RLT buffer with 1% b-mercaptoethanol as a collection/lysis buffer (RNeasy micro kit, Qiagen). RNA was extracted following manufacturer’s instructions (RNeasy micro kit ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded using a peristaltic pump in 1 mL (bed volume) of Ni-NTA agarose (QIAGEN) packed in a Poly-Prep® Chromatography Column (Cat #731-1550 ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from frozen Hepa 1-6 cell pellets using the RNeasy Mini kit (74106, QIAGEN) following the manufacturer’s instructions with an on-column DNase digestion step (79254 ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription was performed with 1 µg of template RNA using the Qiagen QuantiTect reverse transcription kit (Qiagen) in a Bio-Rad T100TM Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was then purified from 500,000 to 1 million cells using the miRNeasy Micro kit (Qiagen GmbH) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a minimum of 1 x106 cells per experimental group were processed using the RNeasy Mini kit (#74104; QIAGEN). The mRNA levels were measured with the NanoDrop (DS-11 ...
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bioRxiv - Plant Biology 2023Quote: ... All protein sequences (for accession numbers see Supplementary Table 1) were aligned using CLC Genomics Workbench v20.0.4 (Qiagen) and the phylogenetic tree was constructed with the neighbor-joining method using 1000 bootstrap replicates ...
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bioRxiv - Genomics 2023Quote: ... Total RNA was isolated from 1 million cells per condition using the RNeasy Plus Mini Kit (Qiagen, 74104). mRNA was isolated using the Poly(A ...
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bioRxiv - Biochemistry 2023Quote: ... and grown in LB medium (ampicillin 50 μg mL−1) and plasmid purified (miniprep kit Qiagen, Hilden, Germany). Mutations were confirmed by DNA sequencing (Eurofins Genomics ...
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bioRxiv - Genomics 2023Quote: ... DNA fragments were run on a 1% agarose gel for imaging or purified on QIAquick columns (28115, Qiagen) for sequencing analysis ...
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bioRxiv - Genomics 2023Quote: ... 1 μg total RNA was subjected to reverse transcription with a QuantiTect reverse transcription kit (Qiagen, Germantown, MD). The resulting cDNA and Fast SYBR green master mix (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... An amount of 1 µg of RNA was reverse transcribed using Omniscript Reverse Transcription (RT) Kit (Qiagen, #205113) together with random oligonucleotides (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C for 1 h and purified by affinity chromatography using a 5 ml Ni-HP column (Qiagen). Flow through with pure AstaPo1 was collected and dialyzed overnight against the buffer containing 50 mM Tris pH 7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell pellets were washed with 1× PBS and RNA were extracted using the RNeasy plus mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted from 1 x 106 transduced HEK293T cells with the RNeasy Plus Mini Kit (Qiagen, #74134) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% CA630) and rotated at 4°C for 2 hours before addition of His-beads (Ni-NTA, QIAGEN). After continuous rotating for another 2 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μL of bisulfite treated DNA was amplified for the regions of interest using PyroMark PCR kit (Qiagen) with final primer concentration of 0.4 μM ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated by phenol:chloroform:isoamylalcohol (25:24:1 pH 8) extraction followed by Qiaquick PCR Purification (QIAGEN, Germany). Purified DNA was then analyzed on a 1.5% agarose gel and on Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then disrupted by sonication and the supernatant was mixed with 1 mL Ni-NTA agarose (QIAGEN) and incubated for 1 h at 4 °C with gentle shaking ...