Labshake search
Citations for Qiagen :
1101 - 1150 of 3908 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The supernatant was added to a 50 mL Falcon tube containing 5 ml Ni-NTA suspension (Qiagen) resuspended in 2 mL solution A and left to settle on a tilting table for 1 hour at RT ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from 5 × 106 T cells using the RNeasy® Mini Kit (Qiagen, Cat. 74106). RNA extraction was performed following the instructions from the “Purification of Total RNA from Animal Cells Using Spin Technology” protocol given in the RNeasy® Mini Handbook ...
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bioRxiv - Microbiology 2024Quote: ... NGS libraries were constructed using Enzymatics 5× WGS fragmentation mix and WGS ligase reagents (Qiagen, Hilden, Germany), and then libraries were sequenced on HiSeq X Five platform in Macrogen Japan (Tokyo ...
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bioRxiv - Molecular Biology 2024Quote: Total RNAs were extracted from 5×105 cultured cells using an RNA isolation kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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Microbial iCLIP2: Enhanced mapping of RNA-Protein interaction by promoting protein and RNA stabilitybioRxiv - Molecular Biology 2024Quote: ... one steel bead (5 mm diameter) was added and placed into a pre-cooled TissueLyser Adapter (Qiagen). The samples underwent two rounds of cell lysis ...
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bioRxiv - Immunology 2024Quote: ... The digested mixture was then loaded onto a pre-equilibrated 5 mL Ni-NTA Superflow column (Qiagen) to remove the cleaved His-tag ...
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bioRxiv - Neuroscience 2024Quote: ... The pellet was resuspended in 340μl of lysis buffer containing 5 mM EDTA and proteinase K (Qiagen) and incubated at 55°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 mm punch biopsies were excised from trunk nodules and immersed in 2.5 ml RNALater Reagent (Qiagen). Incisions were treated with iodine and oxytetracycline ...
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bioRxiv - Bioengineering 2024Quote: ... Gibco) with 5% (w/v) glucose (Fisher ChemicalTM) pH 7.4 and nuclease-free water (NF water, Qiagen). Then ...
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bioRxiv - Immunology 2020Quote: ... Pellets were lysed by vortexing for 1 minute in 350uL cold supplemented RLT buffer (RLT + β-MeOH) at 4°C and lysates homogenized using QIAshredder columns (Qiagen). RNA was then extracted from these samples using the RNeasy Plus Micro kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... after which we select the top 250 genes for which the correlation coefficient was positive and the top 250 genes for which the correlation coefficient was negative. The final step of the pipeline (Fig. 1 (4)) analyzes these genes by using Ingenuity Pathway Analysis (IPA, QIAGEN, [27]) to interpret the canonical pathways.
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bioRxiv - Cancer Biology 2021Quote: ... After 2 hours we extracted the total RNA (RNeasy mini kit, Qiagen, cat. n. 74104) from 40 animals for each of the 3 experimental replicates (total ...
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bioRxiv - Cell Biology 2020Quote: RNA from 2×107 cells was isolated using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), cDNA was generated by reverse transcription on CFX96 real-time system (BioRad ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from 2 μl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 2 minutes (40 oscillation per second) on a bead shaker (Qiagen Tissue lyser)-centrifuged at 1000g for 5 minutes at 4 °C to remove debris ...
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bioRxiv - Molecular Biology 2020Quote: ... and gonad were collected and stored separately in 2-mL tubes containing RNA later (QIAGEN) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... coli* culture from each growth condition were stabilised in RNAProtect Bacteria Reagent (2 mL; Qiagen) and cells pellets were frozen at −80°C prior to processing ...
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bioRxiv - Bioengineering 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4 °C for 2 h with rotation.
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bioRxiv - Biochemistry 2021Quote: ... the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210, QIAGEN), incubated at 4°C for 2 h with rotation ...
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bioRxiv - Microbiology 2020Quote: RNA was isolated from U-2 OS cells with the RNeasy minikit (Qiagen, Hilden, Germany). 0.5µg RNA was reverse transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara ...
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AMPK is elevated in human cachectic muscle and prevents cancer-induced metabolic dysfunction in micebioRxiv - Molecular Biology 2022Quote: ... the muscles were homogenized (2 × 30 sec, 30 Hz, TissueLyser II bead mill, Qiagen, USA) in a modified GSK3-buffer (10% glycerol ...
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bioRxiv - Genetics 2022Quote: ... RNA was collected from 2 x 105 pelleted cells using the RNeasy mini kit (Qiagen). qPCR using primers specific for ERAP1 or TNFSF12 were used to validate knockdown relative to 18S rRNA.
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bioRxiv - Microbiology 2021Quote: ... The samples were then homogenized using a Vortex-Genie 2 and vortex adapter (QIAGEN®) at the highest setting for 10 minutes.
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bioRxiv - Genomics 2022Quote: ... Lysate was spun down and supernatant was mixed with 2 ml Ni-NTA resin (Qiagen) per 50 ml sample and incubated for 1h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 RNA was quantified by RT-qPCR with the QuantiTect Kit (Qiagen, 204443) with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: RNA was isolated from 2 x 106 cells with the RNeasy Plus Mini kit (Qiagen) and reversely transcribed with SuperScript III (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and collected into a PCR tube containing 7 µL of 2×TCL lysis buffer (Qiagen) with 2% v/v 2-mercaptoethanol (Sigma) ...
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bioRxiv - Genetics 2021Quote: ... Other ventriculus sinister sections were pestled by a grinding apparatus (Schwingmühle TissueLyser 2, Qiagen, Germany) and stored at −80°C for detection ...
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bioRxiv - Neuroscience 2023Quote: ... PCR primers were designed using the PyroMark Assay Design 2.0 software (Qiagen, Supplementary Table 2). The assay for MBP was tested for its sensitivity using the EpiTect PCR Control DNA Set (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... siRNA-transfection reagent complexes were generated using 2 μL HiPerfect transfection reagent (Qiagen, Germantown, MD) and 25-50 nM siRNA (non-specific control siRNA (sc-37007 ...
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bioRxiv - Plant Biology 2023Quote: ... Christ) and ground to powder at 30 Hz for 2 × 45 s (TissueLyzer II, Qiagen). DNA was extracted according to the manufacturers instructions ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was run on 2% agarose gel and extracted using QIAquick Gel Extraction Kit (QIAGEN). Fragments and backbone were ligated using T4 DNA ligase (Cat# M0202 ...
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bioRxiv - Molecular Biology 2023Quote: ... The 15 µl reactions each contained 2 × reaction mix from the Multiplex PCR Kit (Qiagen), 5 µg Bovine Serum Albumin ...
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bioRxiv - Microbiology 2023Quote: ... then 50 mg transferred to 2 ml Plant Pro tissue disruption tubes (Qiagen Ltd, U.K.). DNA was extracted following manufacturers protocol ...
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bioRxiv - Microbiology 2024Quote: ... run using the QIAcuity One Digital PCR System (Qiagen, 2-plex Device, Cat. No. 911001). The following conditions were used for the one-step cycling dPCR program ...
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bioRxiv - Microbiology 2024Quote: ... or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0, Qiagen, Toronto, ON, Canada). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... clusters (0-2) were predicted with Ingenuity Pathway Analysis (IPA; version 90348151, Ingenuity Systems, Qiagen), using the cluster marker gene lists (adj ...
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bioRxiv - Immunology 2024Quote: ... using primers (Supplementary Table 2) and purified the products using QIAquick PCR Purification Kit (Qiagen). PCR-products were Sanger sequenced and knockout scores were determined using the ICE analysis tool (https://ice ...
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bioRxiv - Microbiology 2024Quote: ... or AZD5582 in the presence or absence of KL-2 using an RNeasy kit (Qiagen), with the optional on-column deoxyribonuclease I digestion step ...
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bioRxiv - Immunology 2024Quote: ... Sorted cells (2-4x104 per sample) were resuspended in 350 µl RNeasy Lysis Buffer (QIAGEN), and RNA was extracted using the QIAGEN RNeasy Mini kit (QIAGEN) ...
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bioRxiv - Genomics 2023Quote: ... Cell-free RNA was isolated from between 2-8 mL following manufacturer’s instructions (Qiagen, 55114). Isolated RNA was DNase-treated (Lucigen ...
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bioRxiv - Genetics 2023Quote: ... we extracted genomic DNA from 2 million cells using DNeasy Blood & Tissue Kit (69504, Qiagen) and performed droplet digital PCR (ddPCR ...
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bioRxiv - Plant Biology 2023Quote: Genomic DNA was extracted from 2-week old seedlings with DNeasy Plant Mini Kit (Qiagen) and further purified with Isolate II Gel and PCR Clean-up Kit (Bioline) ...
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bioRxiv - Microbiology 2024Quote: ... with 200 µl of the resulting supernatant transferred into 2 ml EZ1 sample tubes (Qiagen). 12 µl of Proteinase K was added to the supernatant ...
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bioRxiv - Immunology 2023Quote: ... DNA was isolated for each fraction using EZ1&2 DNA Blood 350µL kits (Qiagen, Hilden) and the EZ1 Advanced XL automated system (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... The initial homogenization was performed mechanically (2 metal beads/sample) using a TissueLyserLT (#85600, Qiagen) homogenizer (50 oscillations/s for 10 min) ...
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bioRxiv - Microbiology 2023Quote: ... MAYV samples were homogenized at 30 Hz for 2 min in a TissueLyser II (Qiagen) and centrifuged for 30 sec at 11,000 rpm ...
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bioRxiv - Genomics 2023Quote: ... RNAse A treatment was conducted by adding 2 uL of RNAse A 100ug/mL (Qiagen) and incubating the samples at 37°C for 15 min ...
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bioRxiv - Plant Biology 2024Quote: ... all samples were ground for 2 minutes at 25 Hz (TissueLyser II, QIAGEN, Hilden, Germany). A dilution series was performed on this mixture with MgCl2 (10 mM ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified from 2 % agarose-gels using QIAquick Gel Extraction Kit (QIAGEN, 28706) for Sanger sequencing (Microsynth).