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Citations for Qiagen :
1101 - 1150 of 1696 citations for 2 Chloro 3 6 chlorohexanoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... genomic DNA from 2×107 cells was extracted by DNeasy blood & tissue kit (Qiagen, Cat.69504) and sgRNA cassettes were PCR amplified for illumina NGS 53.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was applied to a 2-ml column of Ni-NTA-agarose (Qiagen, Hilden, DE) at a flow rate of 1 ml min−1 ...
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bioRxiv - Molecular Biology 2022Quote: ... rotated at 4°C for 1.5-2 hr in 0.75 mL equilibrated Ni-NTA Agarose (Qiagen), followed by packing pre-equilibrated Poly-Prep Chromatography Columns (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted from the Caco-2 cell lines using a RNeasy Mini Kit (QIAGEN SA) with a DNase I step to eliminate DNA contaminants ...
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bioRxiv - Neuroscience 2024Quote: Total RNA from iPSC-derived microglia (Supplementary Table 2) was extracted using RNeasy Plus kit (Qiagen). Library construction and sequencing were performed by Novogene ...
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bioRxiv - Immunology 2023Quote: DE genes between clusters 2 and 4 were fed to Ingenuity Pathway Analysis software from Qiagen. Enrichment pathways were sourced from Ingenuity core enrichment pathways.
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bioRxiv - Genetics 2023Quote: ... PCR amplicons were resolved by 2% agarose gel electrophoresis and gel-purified (QIAGEN Gel Extraction Kit). Libraries were quantified by qPCR using the NEBNext Library Quant Kit (NEB) ...
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bioRxiv - Pathology 2023Quote: ... at 35 Hz for 2 minutes with a sterile stainless steel 0.5 mm metal bead (Qiagen). Homogenized samples underwent depletion of host and bacterial ribosomal RNA using a modified previously published protocol (40) ...
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bioRxiv - Microbiology 2023Quote: ... each cell cultures (4ml) are treated with 2 volumes of RNA Protect Bacteria reagent (Qiagen, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were then treated with DpnI for 1-2 hr and then purified (QiaQuick, Qiagen) before use in library construction.
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of purified genomic DNA was then bisulfite converted using the EpiTect Bisulfite Kit (Qiagen). PCR was performed on bisulfite-treated gDNA using HotStarTaq DNA polymerase (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... each spot was collected into a 1:2 mix of LBS and RNAprotect Bacteria reagent (Qiagen), incubated at RT for 5 minutes ...
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bioRxiv - Genomics 2023Quote: ... PCR 2 products were purified via gel extraction using the Qiaquick Gel Extraction Kit (Qiagen #28706X4) and prepared for sequencing as described in Illumina’s library denaturation and dilution manual ...
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bioRxiv - Cancer Biology 2023Quote: Each qPCR reaction consisted of 5 μL of 2× QuantiNOVA SYBR Green PCR master mix (Qiagen), 0.5 μL of 10 μM forward and reverse primers each ...
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bioRxiv - Cell Biology 2023Quote: ... The cells sediment was added to 2 mL Red Blood Cells Lysis Solution (Qiagen, Duesseldorf, Germany) at room temperature for 5 min to remove red blood cells ...
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bioRxiv - Systems Biology 2023Quote: ... About 1 OD/mL cells were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, 76506), and the cell pellet was collected by centrifuging at 2500 g for 8 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Take 2 μg of template DNA and bisulfite treat according to the EpiTect Bisulfite Kit (QIAGEN) manual ...
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bioRxiv - Plant Biology 2023Quote: ... Around 2 micrograms of DNA was used for DNA bisulfate conversion by EpiTect Bisulfate kit (QIAGEN). The converted DNA served as a template for amplification ...
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bioRxiv - Biochemistry 2023Quote: PCR products were purified from 2% agarose gel and extracted by QIAquick Gel Extraction Kit (Qiagen) following the manufacturer’s protocol.
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bioRxiv - Genomics 2024Quote: ... DNA was extracted from 1 or 2 bumblebee legs using the DNeasy Blood & Tissue kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... at 55 °C for 2 hr before recovered using a miniElute PCR purification kit from Qiagen. ChIP-qPCR was performed using primers listed in Supplementary Table 5 and PowerUp SYBR green master mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were transferred to a 2 ml tube with 0.5 mm glass beads (Qiagen, Germantown, Maryland) and bead beat for 10 min at maximum speed on a Vortex-Genie 2 (Scientific Industries ...
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bioRxiv - Synthetic Biology 2024Quote: ... The soluble fraction was loaded onto a column containing 2 mL of Ni-NTA resin (Qiagen) previously equilibrated with 20 mL lysis buffer ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in 2 volumes (g pellet:ml volume) of RNA protect Tissue (Qiagen, Germany) and homogenised by passing 3 times through a needle (26G) ...
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bioRxiv - Evolutionary Biology 2024Quote: Total SARS-CoV-2 RNA was extracted by using the Analytik Jena Kit (QIAGEN, Hilden, Germany), followed by reverse transcription into cDNA with a high capacity cDNA reverse transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... and dact1/2-/-fish were collected and RNA extractions were performed using RNeasy Mini Kit (Qiagen). cDNA was generated using High Capacity cDNA Reverse Transcription Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was incubated for 2 h at 4°C while rotating with Glutathione superflow beads (Qiagen) for GST-tagged CMK-1 variants or nickel-nitrilotriacetic acid beads (Ni-NTA ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from about 2-4×106 cells using the RNeasy Mini Kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... KCl (to a final concentration of 0.3 M) and 2 ml of Ni-NTA beads (Qiagen) were added and incubated at 40 °C for 1.5 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 2% Triton-X 100) and lysed with 200 μL of AL lysis buffer (Qiagen, 19075). DNA was precipitated by adding 1 mL of 100% ethanol supplemented with 100 mM of sodium acetate ...
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bioRxiv - Genomics 2021Quote: ... PI-negative fraction was sorted in each well of a 96 well plate containing 3 μL of PBS (Qiagen, REPLI-g SC Kit) with 0.2% Tween 20 ...
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bioRxiv - Developmental Biology 2020Quote: ... rhesus fibroblast cell line (n=1), pri-CTB (n=1, rh090419) and TSCs (n=3 rh121118, rh052318, cy091318) using a FlexiGene DNA Kit (Qiagen, cat no: 51206) and quantified using a Nanodrop™ One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... and sunflower_KTI _R (5’ – CTACTCAGATTGAACAGAAGCCAC- 3’were designed and used in a PCR reaction with total DNA extracted (DNeasy Plant Mini Kit, Qiagen, Valencia CA USA) from sunflower leaf tissue ...
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bioRxiv - Microbiology 2022Quote: ... we added 500 μL of TRIzol and one 3-mm glass bead to the microtubes with mosquitoes and then homogenized the samples in a TissueLyser® (QIAGEN, Hilden, Germany) at 30 Hz for 3 min ...
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bioRxiv - Plant Biology 2021Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen; catalog no. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Microbiology 2022Quote: Total mouse lung RNA was extracted at days 0 and 3 post-challenge using the RNeasy Mini Kit as described above (QIAGEN, Cat. No 74004) and purified with the QIAquick PCR Purification Kit (QIAGEN ...
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bioRxiv - Systems Biology 2022Quote: Total RNA was isolated from whole brain tissue from 3 biological replicates per strain for 45 strains of the HRDP using QIAzol (Qiagen, Valencia, CA, USA). The brain was split sagitally and half of the brain was used for RNA sequencing ...
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bioRxiv - Immunology 2023Quote: Whole livers were harvested at indicated timepoints and placed into a 7 ml homogenizer tube (Omni International Cat. No. 19-651) containing 3 mL sterile PBS and one 5 mm stainless steel bead (QIAGEN Cat. No. 69989). Spleens and single granulomas were harvested at indicated timepoints and placed into a 1 ml homogenizer tube (Fisher Brand Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5μl of diluted cDNA (1:3 - nuclease-free water) was used as template in 10μl real-time PCR reactions (Qiagen: ‘SYBR Green PCR kit’) to assay specific transcripts (BioRad ...
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bioRxiv - Physiology 2023Quote: ... Ganglia in 1 mL of TRIzol reagent were homogenized using a TissueLyzer II bead mill (one 5 mm stainless steel bead per tube, 30 s-1 frequency for 3 minutes; Qiagen Inc. Hilden, Germany). The homogenates were incubated with 200 µL chloroform and centrifuged for 15 minutes at 12000 x g to isolate the protein-containing organic phase ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant backbone without the Puromycin gene was purified using the QIAquick PCR & Gel Cleanup Kit (Qiagen 28506. A DNA block (Table 3) containing the Blasticidin resistance gene sequence was synthetised (Integrated DNA Technologies ...
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bioRxiv - Genetics 2024Quote: ... comprising 1x reaction buffer QIAGEN Multiplex PCR Master Mix (containing dNTPs, HotStartTaq DNA polymerase and 3 mM MgCl2 as final concentration; QIAGEN, Venlo, the Netherlands), 0.05 µM tailed forward primer (see Suppl ...
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bioRxiv - Bioengineering 2020Quote: ... Approximately 150 mg FW of frozen plant material was disrupted with a glass and a metal bead (Ø 3 mm; QIAGEN GmbH, Hilden, Germany) in a TissueLyser (MM400 ...
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bioRxiv - Plant Biology 2022Quote: RNA samples were isolated from seedlings at each time point with 3 biological replicates using Qiagen Plant RNA extraction kit (Qiagen, Germany; Cat. No. 74904). A total of 18 cDNA libraries were prepared following the standard BGISEQ-500 RNA sample preparation protocol and sequenced by the DNBseq platform (BGI ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated microvascular cells were stained for flow cytometric analysis or were additionally processed with myelin removal beads (Miltenyi 130-069-731) and 3 sequential positive selections with CD31 microbeads (Miltenyi 130-097-418) for RNA isolation (Qiagen RNeasy Micro Kit 74004). cDNA library preparation used Oligo-dT at 60 million clusters/sample (University of Chicago Genomics Facility).
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bioRxiv - Cell Biology 2020Quote: ... His6-SUMO-WASHC2C-5-SNAP was purified using lysis buffer #2 and Ni-NTA-agarose beads (Qiagen). The protein was incubated with Ni-NTA beads and washed with 50 mM imidazole ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were maintained in sphere-forming cultures for 2 weeks prior to RNA collection (RNAeasy Kit, Qiagen). TGFB1 was replenished when placing mOSE cells in sphere-forming conditions.
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bioRxiv - Molecular Biology 2022Quote: ... Each reaction of the HRM assay included 6.25µl of Type-IT 2 x HRM buffer (QIAGEN, Germany), primers specific for the uidA E.coli housekeeping gene and molecular grade water was added to make a final volume of 12.5µl ...
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bioRxiv - Microbiology 2022Quote: ... 2×109 bacteria were pelleted and DNA was extracted using a DNeasy Blood and Tissue kit (Qiagen). The optional RNase step in the protocol was performed to remove contaminating RNA ...
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bioRxiv - Systems Biology 2020Quote: ... Cell pellets were transferred to 2 ml polypropylene tubes and disrupted twice using a TissueLyser II (Qiagen), adding the same volume of acid-washed glass beads (Sigma-Aldrich ...