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Citations for Qiagen :
1001 - 1021 of 1021 citations for Recombinant Human LAMP1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Functional pathway and network analysis of differentially expressed proteins were performed using Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City, CA, USA). The pathway and network score were based on the hypergeometric distribution and calculated with the right-tailed Fisher’s exact test.
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bioRxiv - Cell Biology 2023Quote: ... Cat# 28-9537- 66) was used and Streptavidin tagged proteins were purified using Strep-Tactin® Superflow® Plus cartridge (QIAGEN). Columns were attached to MINIPULS® 3 peristaltic pump (Gilson ...
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bioRxiv - Molecular Biology 2023Quote: ... 40% of the protein material of each fraction was analyzed by Western blot using an anti-his antibody (Penta-His antibody, Qiagen #34660). Detection was performed with a secondary antibody conjugated with horseradish peroxidase (Anti-Mouse IgG Peroxidase antibody ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... Frozen cells were thawed on ice and used for protein purification with nickel-nitrilotriacetic acid (Ni-NTA) Fast Start column as recommended by the manufacturer (Qiagen, Germany). The bound proteins were eluted from the column with elution buffer containing 50 mM NaH2PO4 ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was purified from soluble fraction under native conditions using Ni2+– NTA affinity chromatography (Ni2+ beads obtained from Qiagen, #30210), followed by a second step of purification involving gel filtration chromatography using Superdex 200 10/300 GL (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Zoology 2019Quote: ... frozen and stored in liquid nitrogen for extraction of total protein and diene conjugates or placed in QIAzol Lysis Reagent (Qiagen, Hilden, Germany) and frozen in liquid nitrogen for RNA isolation ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from cells treated for 48 h with 1 mM aspirin and 4 μM regorafenib using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen Cat# 80004) as per manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell and molecular functions and canonical pathways of these proteins were identified using Ingenuity® Pathway Analysis (IPA®) (Qiagen, Germany).
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in the plate using 1 mL of RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen, Hilden, Germany). Experiments were performed in triplicate.
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bioRxiv - Immunology 2023Quote: Total DNA and mRNA were isolated from the intestine samples collected during fish necropsy with the AllPrep DNA/RNA/Protein Kit (Qiagen, Hilden, Germany), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 80% of the cell suspension was lysed in 6M guanidine-HCl buffer and His6-tagged proteins were captured on Ni2+-NTA resin (Qiagen; cat. #30210). Pull-down eluates and inputs were separated on SDS-PAGE gels and analyzed by immunoblot ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma membrane proteins were purified from cultured astrocytes using a commercially available kit (Qproteome Cell Compartment Kit; Qiagen, Inc, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The left hippocampus was homogenized on ice in the lysis buffer from the AllPrep® DNA/RNA/Protein Mini Kit (QIAGEN, Hilden, Germany). A sample from the lysate was used to quantify total DNA and RNA content using the appropriate Qubit® Fluorometer kit (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... This construct was co-transfected with the pVSV-G retroviral coat protein expression vector into GP2-293 packaging cells using Effectene (Qiagen Sciences, Inc.; Germantown, MD). At 24 h and again at 48 h post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... RNA and protein were digested in 40 mM Tris-HCl pH 6 and 10 mM EDTA by adding first RNAse A (Qiagen; #19101, 1.5 hours, 37°C), followed by Proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were mechanically dissociated in lysis buffer and total RNA was extracted using the AllPrep DNA/RNA/protein mini Kit (Qiagen, USA, Cat. No. / ID: 80004) according to the manufacturer’s protocol ...