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Citations for Qiagen :
1001 - 1050 of 3324 citations for PI 3065 CAS 955977 50 1 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... and supplemented with 1X EDTA and 1X Halt Protease inhibitor cocktail (Thermo-Scientific, CAT No.:78440) and were lysed using bead beating (50 Hz for 3 five-minute cycles, TissueLyzer II (Qiagen) with 0.1 mm silica beads) ...
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bioRxiv - Cell Biology 2021Quote: Transgenic parasites were generated by electroporation of sorbitol-synchronized ring-stage parasites with 50-100 μg of purified plasmid DNA (Qiagen). To select for the plasmids pArl-PfCentrin1-GFP and p3-NLS-L3-mCherry ...
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bioRxiv - Neuroscience 2022Quote: Genomic DNA was isolated from 50 mg of GFM frozen brain tissue by using the Qiamp DNA mini kit (Qiagen) following the manufacturer protocol ...
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bioRxiv - Genomics 2022Quote: ... Amplified libraries were then purified using 1.5x ratio of SPRI beads to remove adapter dimers and fragments smaller than 90-100bp and eluted in 50 μL of EB (Qiagen) buffer after 10 minutes incubation at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... RNA extraction from 50 μl of nuclei suspension from FACS (containing 5000 sorted NeuN+ nuclei) was conducted using RNeasy Mini Kit (74104, Qiagen) and RNase-Free DNase Set (79254 ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA was obtained from pools of approximately 30-50 heart tubes of each genotype using an RNeasy Plus Micro Kit (QIAGEN) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
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bioRxiv - Cell Biology 2021Quote: ... Then approximately 50 ng of gDNA was harvested from each colony using the QIAamp DNA Micro Kit (Qiagen, no. 56304). The gDNA was PCR amplified in a 50 µl reaction targeting the STADT barcode ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR mixtures were assembled using 50 ng or 5 µl total RNA and QuantiTect Probe RT-PCR Kit (Qiagen). Amplification and analysis were performed using QuantStudio3 system (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluted pellets were de-crosslinked at 65°C overnight and purified in 50 μl Tris-EDTA buffer using the QIAquick PCR Purification Kit (Qiagen). Differences in the DNA content from every immunoprecipitation assay were determined by real-time PCR using the ABI 7700 sequence detection system and SYBR Green master mix protocol (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 50 µl using 2.5 U of HotStar HiFidelity DNA polymerase (Qiagen,Germany), 2.5 µl of 10 µM primers ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted and purified from pooled scrambled control and genotyped cldn7b F0 knockout larvae (n=50 per group) at 7dpf using the RNeasy Plus Micro Kit (QIAGEN), followed by cDNA synthesis with the SuperScript IV VILO Master Mix Kit (ThermoFisher) ...
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bioRxiv - Genomics 2020Quote: ... The ethanol was then poured out and the pellet was air-dried for 15 minutes before resuspension in 50 to 100 μL of DNA Hydration Solution (Qiagen). DNA was sequenced with paired end Illumina sequencing at 30x coverage.
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bioRxiv - Microbiology 2020Quote: ... pentatrifurcatum 38,39 cells in culture dishes (50 Petri dish cultures) using MagAttract high-molecular-weight (HMW) DNA kit (Qiagen, MD), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Genomic DNA was isolated from 2 clones of each strain corresponding to 50 OD600 units with the Genomic DNA Buffer Set and Genomic-tip 100/G (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The Bacmid DNA pellet was then dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Microbiology 2020Quote: ... The DNA template for standard curves was prepared by PCR performed in 50 μl reactions containing 25 μl Taq PCR Mastermix (Qiagen), 19 μl PCR-certified water ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant of the bacteria lysate was transferred into new 50 ml Falcon tubes and incubated with Ni-NTA (Qiagen) at 4 °C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was isolated from approximately 50 mg of liver tissue or snap frozen cells using DNeasy Blood & Tissue Kit (Qiagen) using the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... All plugs were incubated twice (2 hours incubation followed by an overnight incubation) at 50 °C with freshly prepared 167 μl Proteinase K (Qiagen) in 2.5 ml lysis buffer (BioNano Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... clones were isolated from a 5 ml High Salt (HS) Luria Bertani (LB) culture containing ampicillin (50 μg/ml) using the QIAprep Spin Miniprep Kit (Qiagen). Inserts in pGEM-T Easy were sequenced at Aberystwyth University’s Translational Genomics Facility and the sequence trace was analysed using FinchTV 1.4.0 software [45] ...
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bioRxiv - Molecular Biology 2020Quote: Remaining cell cultures were spun down at 4000 rpm for 5 min and the cell pellet resuspended in 50 μL nuclease-free water (Qiagen). Total RNA was extracted using a hot acidic phenol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... into parasites already ectopically expressing NLS-mCherry (previously provided by Markus Ganter (Simon et al., 2021a)) with 50–100 μg of purified plasmid DNA (QIAGEN). Plasmid precipitation was done prior to transfection as stated above ...
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bioRxiv - Microbiology 2023Quote: Total DNA for SCS_HM2 sediments (∼0.5 g) were extracted using the PowerSoil DNA Isolation Kit (Catalog No. 12888-50, Qiagen, Germantown, MD) following the user’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50 μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR reactions for each primer set were performed on RNA (50-75 ng/ul) from at least two biological replicates of the mutant allele and control (QIAGEN OneStep RT-PCR Kit ...
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bioRxiv - Biochemistry 2023Quote: ... were used as per manufacturer’s instructions to clean up the reacted plasmid DNA and eluted in a 50 μL volume in buffer EB (Qiagen).
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bioRxiv - Biochemistry 2023Quote: ... SUMO protease was added at a final concentration 1/10 and the mixture was dialyzed overnight at 4 °C against the buffer DB6 (50 mM Tris-HCl pH 8, 150 mM NaCl, 10 mM imidazole) and applied on a NiNTA column (Qiagen) equilibrated in the DB6 buffer ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from bacterial cells using the RNeasy mini kit (5 mL culture) and RNeasy maxi kit (50 mL culture) (Qiagen). RNA concentrations were measured using a Nanodrop spectrometer (ThermoScientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... resuspended in 50 ml of distilled water and the protocol was modified to include 0.1 mm glass beads (Qiagen, Germany) to increase DNA extraction by mechanical lysis ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from fecal pellets (100 mg, n=5 per group) by the QIAmp Power Fecal DNA kit (12850-50, Qiagen). The V4 region of 16S rRNA gene was amplified ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were collected continuously into 50 mL conicals containing 20 mL RNAlater (saturated ammonium sulfate, 20mM EDTA, pH 5.2; QIAGEN #76106) and a stirring magnetic stir disc (V&P Scientific #772DP-N42-5-2) ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Microbiology 2022Quote: ... and His-ZccR eluted with elution buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole) according to the QIAexpressionist protocol (Qiagen) for purification of native proteins with histidine tags ...
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bioRxiv - Genomics 2022Quote: ... We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50, MoBio – now QIAGEN PowerBead) using sterile tweezers ...
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bioRxiv - Cell Biology 2023Quote: ... and added to appropriate volumes of buffer RLT and homogenised at 50 Hz for 5 minutes using the TissueLyser LT (Qiagen), until all tissue was homogenised ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was then air dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Neuroscience 2023Quote: ... Individual microglial cells were cut and dropped into an inverted microcentrifuge cap containing 50 μl buffer RLT (RNeasy Micro Kit - Qiagen) and 1% β-Mercaptoethanol.
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bioRxiv - Cancer Biology 2024Quote: ... more than 50% of RNA fragments being greater than 200 nucleotides (DV200) after extraction with the AllPrep DNA/RNA Universal Kit (Qiagen) during bulk RNA-seq library preparation ...
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bioRxiv - Genomics 2024Quote: ... 50 ml of overnight culture were harvested to purify each random library with a midi prep (Plasmid MIDI Kit, Qiagen).
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bioRxiv - Immunology 2024Quote: GM-MØ (1 × 106 cells) were transfected with a human GSK3A-specific and/or GSK3B-specific siRNA (50 nM) (Dharmacon) using HiPerFect (Qiagen). Silencer Select Negative Control No ...
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bioRxiv - Microbiology 2024Quote: ... Reverse transcription and quantitative PCR were performed in the same closed tube with 50 ng of total RNA per reaction using the Quantitect RT-qPCR Kit (Qiagen). All primers were used at final concentrations of 4 μM ...
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bioRxiv - Microbiology 2024Quote: ... eggs and plant pellets were transferred into the lysis buffer in the Powerbead tubes and vortexed (10 min, 50 Hz) with TissueLyser LT (QIAGEN). DNA was eluted in 40 µL Nuclease Free Water (Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered using a 0.22 μm syringe filter and loaded onto a NiNTA 10/50 column (Qiagen, Germany) and proteins were eluted with an increasing gradient of imidazole ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... at 6 m/s for 50 sec and total RNA extracted with the RNeasy Lipid Tissue Mini Kit (#74804; Qiagen). For SCP (D18 ...
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bioRxiv - Molecular Biology 2024Quote: ... Parasite genomic DNA was extracted from 50 µL of infected blood mixed with 150 µL PBS using the DNeasy Blood and Tissue kit (Qiagen). For pooled transfections ...
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bioRxiv - Physiology 2024Quote: ... weighed and homogenized 1:180 (weight:volume, mg:µL) in homogenization buffer (50 mM Triethanolamine and l mM EDTA) with 2 tungsten beads (Qiagen, Cat. #69997) using a Tissue Lyser (Qiagen Cat ...
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bioRxiv - Biophysics 2024Quote: ... 50 ml of overnight culture were harvested to purify the library in the nicking plasmid pGJJ057 (QIArep Miniprep Kit, Qiagen).
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bioRxiv - Biochemistry 2024Quote: ... The lysate was then cleared by centrifugation at 42,000 ξ g for 50 min at 4°C before being applied to 2 mL of Ni-NTA resin (QIAGEN) in a gravity flow column ...