Labshake search
Citations for Qiagen :
9951 - 10000 of 10000+ citations for C Reactive Protein CRP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the homogenate was loaded into a RNeasy Mini kit (Qiagen) column and RNA purification proceeded according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using the miRNeasy Mini kit (Qiagen, 217004) and subsequently reverse transcribed using the mScript II RT kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: RNA was isolated using the RNeasy Micro Plus Kit (Qiagen) following the manufacturer’s guidelines in Appendix D of the Qiagen RNeasy handbook ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNeasy Mini Kit (74104) was purchased from QIAGEN (Germantown, MD). 10X TAE Buffer and RiboRuler High Range RNA Ladder were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Pathology 2024Quote: Total RNA was purified using a RNeasy micro kit (Qiagen) and used for the preparation of RNA-seq libraries by an Ovation SoLo RNA-Seq Library Preparation Kit (Tecan) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using QIAamp Viral RNA Mini Kit (Qiagen), and cDNA was produced using a ProtoScript® II Reverse Transcriptase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was isolated using the miRNeasy mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Total RNA was extracted using miRNeasy Kit (Qiagen, Cat # 217004). Total RNA quality was assessed with the use of Bioanalyzer ...
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bioRxiv - Genomics 2024Quote: ... purified using the RNeasy Mini Kit (Qiagen, Chatsworth, CA, USA), and used to construct RNA-Seq (transcriptome ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted using the DNeasy Blood & Tissue kit (QIAGEN), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were purified using PCR clean-up kits (Qiagen). The inserts and backbones were assembled by NEBuilder HiFi DNA Assembly Cloning Kit in a 10ul reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA pellets were washed 3X with 70% ethanol and hydrated in pre-warmed (37°C) Elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5. Cat. 19086) and incubated on HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA for experiments was obtained using endotoxin-free plasmid purification kits (NucleoBond Xtra Midi EF, Takara, cat. 740420.10 or Qiagen EndoFree Plasmid Maxi Kit, cat. 12362); concentration and purity were assessed with spectrophotometry and agarose electrophoresis.
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bioRxiv - Evolutionary Biology 2020Quote: ... Genomic DNA was extracted for whole-genome sequencing from samples using the MO BIO Ultraclean 96 Microbial DNA kit (now sold as QIAGEN DNeasy UltraClean 96 Microbial kit), following the manufacturer’s recommended protocol ...
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bioRxiv - Microbiology 2024Quote: ... the soil and sediment samples were extracted using the RNeasy® PowerSoil® Total RNA Kit and RNeasy® PowerSoil® DNA Elution Kit (QIAGEN, Germany), while the marine and freshwater samples were extracted using the DNeasy® PowerWater® Kit and RNeasy® PowerWater® Kit (QIAGEN ...
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bioRxiv - Genetics 2020Quote: ... placed in 2ml Eppendorf (Hamburg, Germany) tubes and disrupted with a pre-cooled Stainless-Steel Beads (5 mm) in the TissueLyzer (QIAGEN, Venlo, Netherlands). The genomic DNA (gDNA ...
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bioRxiv - Microbiology 2022Quote: ... Organs were homogenized by high-speed shaking in 2 mL microcentrifuge tubes filled with sterile PBS and 5/7 mm stainless steel beads using TissueLyser LT (Qiagen, Hilden, Germany).
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bioRxiv - Microbiology 2019Quote: ... A total of 25 cycles of PCRs were performed and the PCR products (∼600 bp for 5’-RACR PCR or ∼500 bp for gene-specific PCR) were gel purified (Qiagen, Valencia, CA). A degenerate primer (SAGGTGCAGCTGGTGCAGTCTGG ...
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bioRxiv - Neuroscience 2020Quote: ... from APP/PS1 and NTG mice treated with vehicle of CP2 for 14 months (n = 5 per group) were lysed in QIAzol (Qiagen cat. # 79306) followed by RNA isolation using miRNeasy (Qiagen cat ...
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bioRxiv - Microbiology 2020Quote: ... Tissues were homogenized in 1 ml cell culture medium (see above) and a 5 mm steel bead in a TissueLyser (Qiagen, Hilden, Germany). Fecal samples were vortexed in sterile NaCl and the supernatant was sterile filtered (22µm ...
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bioRxiv - Genetics 2022Quote: ... and extracted and amplified from vitrified blastocysts by thawing in phosphate-buffered saline supplemented with 5% bovine serum albumin and processing with REPLI-g whole genome amplification methods (Qiagen, Cat. #150343). Informed consent was obtained from all participants (custodians of the blastocysts ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis of 100 ng total RNA was performed with miRCURY LNA RT Kit (10 µl volume reaction) which adds a 5’ universal tag of a poly(A) tail to mature miRNA templates (QIAGEN, Germantown, MD). cDNA template was diluted 1:10 ...
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bioRxiv - Neuroscience 2021Quote: ... tMCAO mice at 24 h and 48 h post reperfusion were used for total mRNA isolation according to the protocols of the Isol-RNA Lysis Reagent (5 PRIME, Gaithersburg, USA) and the bead-milling TissueLyser system (Qiagen, Hilden, Germany). QuantiTect® Reverse Transcription Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: Ticks were homogenised in a 2 ml reaction tube with two 5 mm steel beads and 500 μl PBS using a Tissue Lyser II (Qiagen, Hilden, Germany) for 3 min at 30 Hz ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Genetics 2020Quote: ... 30 mg of snap frozen tissues were processed adding 1 ml of QIAzol reagent in presence of one 5 mm stainless steel bead (Qiagen, Hilden, Germany). Total RNA isolation from homogenized tissues was performed using Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... The remaining lysate was then transferred to 15 ml tubes containing 5 ml lysis buffer and 150 μl Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) and incubated for 4 h at room temperature under constant mild agitation ...
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bioRxiv - Neuroscience 2022Quote: ... and 18S rRNA (5’-GAG CGA AAG CAT TTG CCA AG-3’ and 5’-GGC ATC GTT TAT GGT CGG AA-3’) on a Roter-Gene Q (Qiagen, Hilden, Germany) with 2x AmpiGene® qPCR Green Mix Lo-ROX (Enzo Biochem ...
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bioRxiv - Microbiology 2023Quote: ... a 1 mL aliquot was sterile filtered and kept to test conditioned media for IL-10 induction (as above)) and replaced with 5 mL of RNA-protect (Qiagen, Cat. # 1018390), mixed by pipetting ...
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bioRxiv - Immunology 2023Quote: ... Tissue RNA was isolated from preserved samples using 30 minutes of automated homogenization at 30 Hz with 5 mm ball-bearings in RLT Plus Lysis Buffer (Qiagen TissueLyser II), RNA was then isolated using a RNeasy Plus Micro Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... we adapted the same conditions used in Dip-C40 and lysed each nucleus in 150 nL of lysis buffer containing 20 mM Tris pH8/20 mM NaCl/25 mM DTT/0.15% Triton X-100/1 mM EDTA/5 µg/mL Qiagen Protease (Qiagen, cat. no. 19157). After dispensing ...
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bioRxiv - Neuroscience 2024Quote: ... snap-frozen tissue was processed by adding 500 µl of QIAzol reagent in the presence of one 5-mm stainless steel bead (Qiagen, Hilden, Germany). Total RNA isolation from homogenized tissues was performed using a Qiagen RNeasy kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Mosquitoes were homogenised in pools of ≤ ten in 300 µl tissue culture medium using the Qiagen TissueLyser II with 5 mm stainless steel beads (both Qiagen, Manchester, UK) and centrifuged (10,000 rpm/10 min) ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria were then mechanically disrupted using a bead beater homogenizer (PowerLyzer 24, Qiagen; 2 cycles of 45s 5000 rpm / 5 min ice). After an additional 5 min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... His-6X-tagged proteins (30 μg/10 μl beads) in 400 μl of PBS were incubated with Ni-NTA Magnetic Agarose Beads (QIAGEN, Hilden, Germany) at room temperature (RT ...
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bioRxiv - Zoology 2019Quote: ... frozen and stored in liquid nitrogen for extraction of total protein and diene conjugates or placed in QIAzol Lysis Reagent (Qiagen, Hilden, Germany) and frozen in liquid nitrogen for RNA isolation ...
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bioRxiv - Biochemistry 2021Quote: ... The labeled protein in the soluble fraction was purified using Immobilized Metal Affinity Chromatography (IMAC) using standard methods (QIagen Ni-NTA resin). The purified protein was then concentrated to 2 mL and purified by FPLC sizeexclusion chromatography using a Superdex 75 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... coli as an N-terminal histidine-tagged protein isolated from cell lysate after passage through Ni-NTA agarose (Qiagen, Cat. No. 30210). Bound TTRL55P was then eluted by competition with imidazole ...
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bioRxiv - Microbiology 2022Quote: The recombinant His-TvFACPα full-length protein was produced and purified by a standard protocol as suggested by the supplier (QIAGEN, Hilden, Germany) (48 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cell and molecular functions and canonical pathways of these proteins were identified using Ingenuity® Pathway Analysis (IPA®) (Qiagen, Germany).
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bioRxiv - Genetics 2021Quote: ... The final constructs were transformed into BL21 (DE3) for expression and recombinant Fur/mFur proteins were purified using Ni-NTA agarose (Qiagen, California, USA). The proteins induced by the addition of 1 mM IPTG at 16°C for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining 80% of the cell suspension was lysed in 6M guanidine-HCl buffer and His6-tagged proteins were captured on Ni2+-NTA resin (Qiagen; cat. #30210). Pull-down eluates and inputs were separated on SDS-PAGE gels and analyzed by immunoblot ...
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bioRxiv - Plant Biology 2022Quote: ... 5’ and 3’ untranslated regions and open reading frames) for the paralogs were made using the CLC Main workbench 7 (QIAGEN® Aarhus A/S, Aarhus C, Denmark). Sequence alignments were generated for the genomic DNA ...
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bioRxiv - Genomics 2020Quote: ... ~20 ng genomic DNA was amplified using a whole-genome amplification (WGA) kit according to the manufacturer’s instructions (Qiagen REPLI-g Mini Kit, Qiagen, Valencia, CA). The REPLI-g Kit is developed based on multiple displacement amplification (MDA) ...
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bioRxiv - Genomics 2020Quote: ... DNA was extracted using the Zymo Quick-DNA Universal Solid Tissue Prep Kit (No. D4069) following the manufacturer’s protocol (DNA from a few samples was also extracted with the Qiagen DNeasy Blood and Tissue Kit, No. 69504). DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies ...
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bioRxiv - Genomics 2019Quote: ... using the Zymo Quick-DNA Universal Solid Tissue Prep Kit (No. D4069) following the manufacturer’s protocol (DNA from a few samples was also extracted with the Qiagen DNeasy Blood and Tissue Kit, No. 69504). DNA quality was assessed by electrophoresis on 3% agarose gels and DNA concentration was determined by dsDNA HS Qubit Assay (Molecular Probes by Life Technologies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... dispar we used 5 mm pieces of fin clip from each individual sample for 28-29 individuals per site (at 11 sites) to extract DNA with 96-well DNA extraction kits from Qiagen (DNeasy 96 Blood and Tissue Kit) or Macherey-Nagel (NucleoSpin 96 Tissue) ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA of cells co-transfected with a plasmid expressing sgRNA and another expressing SpCas9 was isolated and purified through a commercially available kit (Qiagen, QIAamp DNA Mini Kit Cat# 51304) as per the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of each female was extracted using standard protocols (DNA used for ONT sequencing: Macherey-Nagel NucleoBond HMW DNA kit, DNA used for Illumina sequencing: Qiagen DNeasy 96 Blood & Tissue kit). The long-read sequencing library was prepared using the SQK-LSK109 Ligation Sequencing Kit (ONT ...