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Citations for Qiagen :
951 - 1000 of 1020 citations for Recombinant Human LEP Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were pelleted at low speed (~1000 RPM) and lysed with one mL RTL buffer from an AllPrep DNA/RNA/Protein isolation kit (Qiagen).
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bioRxiv - Biophysics 2020Quote: ... 45 min at 4 °C) and supernatant containing His-tagged proteins were purified by nickel-nitrilotriacetic acid (Ni-NTA) purification (Qiagen). Protein was eluted in a final elution buffer of 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was supplemented with 10 mM imidazole and His-tagged proteins were purified by affinity chromatography on NiNTA column (Qiagen) at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... 250,000 cells were lysed in RLT-plus buffer and RNA was purified with AllPrep DNA/RNA/Protein Mini Kit (#80004, Qiagen) according to the manufacturer protocol ...
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bioRxiv - Biophysics 2022Quote: ... membranes were solubilized in 1.2% N-decyl-β-maltoside (DM) and the protein was purified on a Ni-NTA affinity column (Qiagen). Following His-tag removal ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumour samples were fresh-frozen in liquid nitrogen and stored at -80°C for protein isolation or preserved in RNAlater solution (Qiagen) for RNA isolation ...
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bioRxiv - Systems Biology 2022Quote: The co-immunoprecipitated RNA samples were submitted to protein digestion using proteinase K (Fermentas) and purified using the MinElute Reaction Cleanup Kit (QIAGEN) with a DNase treatment step ...
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bioRxiv - Bioengineering 2024Quote: ... The output of differentially expressed proteins was further investigated using Ingenuity Pathway Analysis® (IPA; Qiagen®, Redwood City, USA). Data was also independently analysed using PEAKS studio (Bioinformatics Solutions® Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected by double centrifugation and 6x His-tagged target protein in the supernatant was purified using the medium by Ni-NTA (Qiagen) affinity chromatography ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from cell lysates of bovine granulocytes and sorted PBMC subsets of three to seven Holstein Friesian cattle using the AllPrep RNA/Protein kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: The libraries midi-preps were digested with HindIII and NheI restriction enzymes and the insert containing the mutated protein was gel purified (MinElute Gel Extraction Kit, QIAGEN) to be later cloned into the AbundancePCA plasmid and BindingPCA plasmids by temperature-cycle ligation ...
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bioRxiv - Microbiology 2023Quote: All proteins from previously frozen samples (tumors and healthy parts of murine colon) were extracted using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). Protein concentrations were estimated using NanoDrop A280 absorbance ...
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bioRxiv - Microbiology 2023Quote: ... Cell debris was removed by centrifugation and His-tagged ClpC1 proteins were purified from supernatants with Ni-NTA agarose beads (Qiagen). After washing in binding buffer supplemented with 20 mM imidazole ...
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bioRxiv - Bioengineering 2023Quote: ... The bands representing the protein hybrid libraries were excised and DNA was purified using the QIAquick Gel Extraction Kit (Qiagen). The DNA concentration was then measured with the Quant-iT dsDNA (HS ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and proteins were extracted from fresh or snap-frozen FACS-sorted 2-5 million splenic B cells using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentrations of all the collected samples were adjusted to 400 μg mL−1 using the Qubit protein broad range assay kits (Qiagen). The Cyclic AMP XP® kit ELISA plates were prepared according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... This clarified extract was used for the purification of the His-tagged proteins using Ni-NTA agarose resin (Qiagen, USA). The agarose resin was washed twice by resuspending in lysis buffer followed by centrifugation at 3000 rpm and removing the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... and the purity of the purified proteins was checked by SDS-PAGE and western blot using anti-His antibody (1:1000, Qiagen) as primary antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleaved sP36 protein was afterwards purified by a second step of affinity using Ni-NTA agarose (Qiagen, Hilden, Germany) in phosphate buffer A.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc ...
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bioRxiv - Biochemistry 2022Quote: Approximately 25 mg of frozen tissues were transferred to 2 mL Eppendorf Protein Lobind tubes containing one 5 mm stainless steel bead (cat# 69989, Qiagen) and 500 µL of lysis buffer consisting of 5% SDS in 50 mM TEAB with protease inhibitors cocktail (cat# A32953 ...
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bioRxiv - Cell Biology 2023Quote: ... significantly regulated human proteins (t-test, p-value ≤ 0.01; described above) were characterized by core analysis in IPA® software (version 68752261, QIAGEN Inc. ...
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bioRxiv - Biophysics 2023Quote: ... Cleaved PCNA was separated from His-SUMO and uncleaved His-SUMO-PCNA by incubating the protein solution with 5 ml Ni-NTA suspension (Qiagen) resuspended in solution D on a tilting table for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Tris–HCl 20 mM (pH 7.5)) by sonication and the His-tagged proteins were purified on a Ni-NTA column (Qiagen Inc.), eluted with imidazole in buffer A but complemented by ATP 1 mM + MgCl2 3 mM in the case of the Vibrio cholerae helicase ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins harvested after 6 days post transfection were purified from the supernatant on a Ni-NTA Superflow column (#30430, Qiagen). Immediately after the elution ...
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bioRxiv - Biophysics 2024Quote: ... and 20 mM imidazole.[50,51] The cells were disrupted via probe sonication (Brandson) and the protein was purified with Ni-NTA affinity chromatography (Qiagen). The nanopores were eluted from the beads with 3× 250 µL fractions of elution buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... The protein was purified under denaturing conditions by nickel-nitrilotriacetic acid (NTA)-agarose affinity chromatography as described by the manufacturer (Qiagen). A polyclonal rabbit antiserum was raised by Labcorp Early Development Laboratories Inc.
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bioRxiv - Genomics 2020Quote: ... for 4 hours to inhibit reporter transgene expression (t4) prior to isolation of DNA and RNA using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen# 80004) according to the manufacturers protocol ...
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bioRxiv - Genetics 2019Quote: ... protein altering inframe InDels (n=28) and missense variants (n=557) were uploaded to Ingenuity pathway Analysis (Qiagen, Venlo, The Netherlands). Additionally ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Immunology 2020Quote: ... Proteins (as well as DNA and RNA) were then extracted from the lysate using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germany) following manufacturers instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cancer samples were snap-frozen in liquid nitrogen and DNA extracted using the AllPrep DNA/RNA/Protein mini kit (QIAGEN, Germany) as previously reported 18 ...
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bioRxiv - Biochemistry 2020Quote: ... Canonical pathways associated with differentially expressed proteins were identified by Ingenuity Pathway Analysis (IPA, Ingenuity Systems, Qiagen, Redwood, CA, USA; www.ingenuity.com). This program is based on an algorithm that uses curated connectivity information from the literature to determine the network of interactions among the differentially expressed proteins and canonical pathways in which they are involved (Calvano et al. ...
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bioRxiv - Plant Biology 2021Quote: ... the cultured bacteria cells were lysed by using a high-pressure cell crusher and the fusion proteins were purified with Ni-NTA resin (Qiagen, www.qiagen.com) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein in the supernatant was purified to 90% purity by a single-step chromatography on Ni++ - metal chelate affinity (Qiagen, UK) columns and eluting at 0.5 M imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... Matrix crystallization screens were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals were obtained in 20% PEG 3350 at 20 °C ...
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bioRxiv - Neuroscience 2020Quote: ... For the UCP4 antibody protein samples were extracted from the paraformaldehyde fixed tissue using the Qproteome FFPE Tissue Kit (Qiagen, Germany). The tissue blocks analyzed here were taken from the anterior cingulate and occipital cortex (as described above ...
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bioRxiv - Immunology 2022Quote: Initial matrix crystallization trials against 576 conditions were performed at room temperature using the sitting drop vapor diffusion method by mixing equal volumes of protein sample and reservoir using a TTP LabTech Mosquito robot and commercially-available screens (Hampton Research and Qiagen). Initial hits were optimized and crystals for unliganded BG24S60A were obtained in 0.25 M potassium chloride ...
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bioRxiv - Biochemistry 2019Quote: ... Bound STAT3 proteins were then labeled with Alexa488-conjugated anti-6 × His antibodies (1 hr incubation, 1:20 dilution, Qiagen 35310). The array was washed and scanned using a GenePix 4400A scanner (Molecular Devices ...
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bioRxiv - Biochemistry 2019Quote: ... The soluble 6His-tagged proteins were purified from the supernatant by affinity chromatography using Ni2+-NTA agarose resin (Qiagen, Hilden, Germany). After several washing steps ...
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bioRxiv - Synthetic Biology 2020Quote: ... and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN, Germany) equilibrated with purification buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... assay targeting the paramyxovirus L protein gene was adapted using primers developed by Tong et al (36) and the Qiagen AllTaq PCR Core kit (QIAGEN, Australia). Amplicons corresponding to the expected size (584 bp ...
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bioRxiv - Cell Biology 2021Quote: ... Single cell clones were grown for immunoblot analysis and those showing loss of ATF4 protein were selected for sequence analysis involving the isolation of genomic DNA (Qiagen, 69504), PCR amplification using KOD Xtreme™ Hot Start DNA Polymerase (Millipore ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from biopsies of normal colonic tissues with the Allprep DNA/RNA/Protein Mini kit (Qiagen, Germantown, MD) and reverse-transcribed into cDNA with the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... bacteria were pelleted at 3000 rcf and purified following the QiaExpress Ni-NTA Fast Start protein purification protocol (Qiagen, Catalog # 30600) under native conditions.
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bioRxiv - Neuroscience 2022Quote: ... Total RNA from cultured mouse neuro 2a cells was extracted using AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen, Hilden, Germany) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Halo protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9 wash buffer I (50 mM NaxPO4 pH 7.0 and 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... dCas9-Cys protein was purified from the supernatant by incubating for 2 h at 4 °C with Ni-NTA agarose (Qiagen #30210). The beads were washed 10 times with 5 ml dCas9-Cys wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Cat# 28-9537- 66) was used and Streptavidin tagged proteins were purified using Strep-Tactin® Superflow® Plus cartridge (QIAGEN). Columns were attached to MINIPULS® 3 peristaltic pump (Gilson ...
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bioRxiv - Immunology 2023Quote: ... Functional pathway and network analysis of differentially expressed proteins were performed using Ingenuity Pathway Analysis (IPA) (Qiagen, Redwood City, CA, USA). The pathway and network score were based on the hypergeometric distribution and calculated with the right-tailed Fisher’s exact test.