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Citations for Qiagen :
951 - 1000 of 1421 citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Viral RNA was purified from 140 mL of cell culture supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue samples were homogenized in 3 mL sterile PBS for 20 seconds on ice using a TissueRuptor homogenizer (Qiagen) with sterile tips ...
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bioRxiv - Bioengineering 2022Quote: ... protein yields of the CNA35 probe were synthesized using E.Coli bacteria before purification using ÄKTApurifier (Cytiva) and a 5 ml Ni-NTA Superflow Cartridge (Qiagen), dialysis with SnakeSkinTM Dialysis tubing with 10 kDa MWCO ...
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bioRxiv - Systems Biology 2022Quote: ... Ten millilitres of culture were pelleted by immediate centrifugation (5,000 × g for 3 min at 4°C) and resuspended in 5 mL of RNAlater (76106; Qiagen). Samples were stored at 4°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was washed three times with ice-cold PBS and suspended in 1 ml TRIzol™ reagent (QIAGEN). Instead ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining recovery was grown overnight in 150 mL LB + chloramphenicol and plasmid DNA was isolated by MaxiPrep (Qiagen). The complexity of the SpCas9 catalytic and PAM domain libraries were estimated to be 77,400 and 292,600 respectively ...
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bioRxiv - Bioengineering 2022Quote: Nuclei were isolated from differentiating white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 uL of Tris-Hydrochloride (Sigma ...
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bioRxiv - Neuroscience 2021Quote: Tissue-gel samples were equilibrated in DNase buffer (750 ml) for 30 min and then incubated with RNase-free DNase1 (2.7 Kunitz units/µl, 50 µl) (Qiagen) in DNase buffer (450 µl ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled ORC were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer B with 10 mM imidazole ...
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bioRxiv - Molecular Biology 2021Quote: ... Peak fractions containing peptide-coupled Cdt1 were pooled and incubated with 0.3 mL of Ni-NTA Agarose Resin (Qiagen) pre-equilibrated in buffer D with 10 mM imidazole for 1.5 hr with rotation at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing SctV9 without higher order aggregates of SctV were selected and incubated with 1.5 mL of Ni2+- NTA resin (Qiagen) pre-equilibrated in Buffer F (6 h ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 20 min under constant rotation with 3,5 ml of pre-washed Ni-NTA superflow beads (Qiagen). Beads were collected by centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... Oocytes and embryos were then washed with D-PBS containing 1 mg/ml polyvinylpyrrolidone (PBS-PVP) and transferred into 50 μl droplets of 0.1% protease (Qiagen) to remove the zona pellucida ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was discarded and 3.5 ml of RLT buffer with B-mercaptoethanol (10 µl for every 350 µl of RLT buffer – Qiagen) was added ...
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bioRxiv - Microbiology 2022Quote: Pellets from 50 ml culture were processed for RNA extraction using the RNeasy PowerSoil Total RNA Kit (Qiagen, USA). A DNase kit (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The lysates were cleared by centrifugation at 10,000 xg for 30 min and added to 1 ml of Ni-NTA agarose (Qiagen) pre-equilibrated with the same lysis buffer ...
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bioRxiv - Microbiology 2019Quote: A total of 250 ng of Qst was incubated with 1 ml Ni-NTA Superflow beads (Qiagen, Hilden, DE) in an end-over-end shaker for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant (enriched with SGs) was transferred to a new 1.5 ml microcentrifuge tube and purified using the RNeasy Plant Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2019Quote: ... or lungs (pneumonia) were harvested and homogenized in 1 mL PBS + 0.1% Triton-X100 using a TissueLyser LT (Qiagen) before plating to LB agar + 5 μg/ml irgasan to enumerate bacterial burden.
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bioRxiv - Immunology 2020Quote: ... or IFNγ (10ng/mL) for 6 hours prior to harvesting and RNA extraction using RNeasy Mini Kit (Qiagen 74106). Next ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 30 min at 45,000 × g at 4°C and incubated with 1 ml of Ni-NTA agarose beads (Qiagen) per 1 l of expression culture for 2 h rotating at 4°C ...
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bioRxiv - Physiology 2021Quote: ... feces were homogenized in 1 mL 80% (v/v) methanol using metal beads (2.4 mm diameter, Askubal) in a tissue lyzer (Qiagen) for 1 min at 30 Hz ...
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bioRxiv - Physiology 2021Quote: ... the supernatant was harvested by centrifugation for 20 min at 10,000 g and then loaded onto 5 ml Immobilized Metal-ion Affinity chromatography (IMAC, Ni-NTA Agarose, Qiagen) pre-equilibrated in 20 mM Hepes pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein was normalized to 2 mg across all samples tested and incubated with 0.25 mL of 50% Ni-NTA resin (Qiagen) for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 12 000 rpm 45 min) and the supernatant was loaded onto a column containing NiNTA resin (1 mL, Qiagen) pre-equilibrated with buffer A (10 mL) ...
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bioRxiv - Microbiology 2020Quote: ... Total RNA was isolated in six biological replicates per strain from 0.5 mL of culture using the RNeasy Protect Bacteria Mini kit (Qiagen) on six consecutive days ...
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bioRxiv - Developmental Biology 2020Quote: ... Femoral vein blood collection (1.0-2.0 mL) was performed on each animal and blood was placed in to PAXgene blood collection tubes (Qiagen). Whole blood was frozen and stored at −80° C for shipment to UCLA for methylation analysis.
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bioRxiv - Bioengineering 2021Quote: Nuclei were isolated from white and brown preadipocytes using an NP-40 based lysis buffer: To 14.7 mL nuclease-free water (Qiagen), 150 ul of Tris-Hydrochloride (Sigma ...
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bioRxiv - Genetics 2021Quote: ... 10 mM imidazole (final concentration) was added to the sample and the solution was bound to 0.5 ml NiNTA resin (Qiagen). The resin was washed with NTA buffer A1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... cells were first washed and pelleted five times with 15 ml of PBS then lysed with QIAzol reagent (Qiagen), RNA was extracted according to manufactures directions ...
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bioRxiv - Immunology 2020Quote: Lung tissue from individual mice were homogenized in 1 mL of TRIsure reagent with TissueLyser II (Qiagen No. 853000) and as per the manufacturer’s instructions and samples were centrifuged for 15 min at 12,000 x g at 4°C to remove cellular debris ...
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bioRxiv - Biochemistry 2019Quote: ... The lysates were centrifuged for 30 min at 45,000 × g and incubated with 1 ml of Ni-NTA agarose (Qiagen) per 1 l expression culture ...
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bioRxiv - Biochemistry 2021Quote: ... The ankyrin repeat domains were purified over a Ni-nitrilotriacetic acid column (2.5 ml column volume) according to the manufacturer’s instructions (QIAgen, Germany). Up to 200 mg of highly soluble ankyrin repeat domains were purified from one liter of E ...
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bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
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bioRxiv - Biochemistry 2021Quote: The LRH-1-10CA-TIF2 complex was concentrated to 5 mg/mL and screened using the Classics screen (Qiagen) and a Phoenix Liquid Handler (Art Robbins Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... One half of clarified cell lysate (2 ml) was applied to 200 μl of Ni-NTA Superflow resin (Qiagen) equilibrated with Ni wash buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen pieces of tissue (~20 mg) were homogenized in 0.5 ml TRIzol in a TissueLyser II (Qiagen, Hilden, Germany) (2 × 2 min at 30 Hz ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were washed with PBS and the pellet was resuspended in 1 ml PBS containing RNAse A (Qiagen, 19101) at a final concentration of 0.2 mg/ml ...
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bioRxiv - Immunology 2021Quote: ... or ectodomain buffer (20 mM Tris, pH 8.5, 500 mM NaCl) and applied to 10 ml Ni-NTA-Sepharose (Qiagen) columns ...
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bioRxiv - Microbiology 2021Quote: ... Samples were spun at 5000 x g for 10 minutes and supernatant was discarded before resuspension in 1.5 mL QIAzol reagent (Qiagen). Screw-cap tubes were prepared for each sample by adding ∼250 µL of sterile beads and the QIAzol suspension mixture was added and incubated for 5 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed to a fresh 50 mL conical and further incubated with 2mL of Talon Beads (Qiagen) for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells suspended at 0.8 × 105 cells/ml were transfected with 50 nM siRNA using HiPerfect transfection reagent (Qiagen) according to the manufacturer’s protocol and analysed after 48 h ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from a 0.5 ml aliquot of the culture using the RNAeasy Protect Bacteria Mini Kit (74524, QIAGEN) with on-column DNAse digestion ...
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bioRxiv - Cancer Biology 2022Quote: ... The femurs were placed with knee-end down in a perforated 0.6 ml tube added 80 μl of RNAlater (Qiagen) and inserted in a 1.5 ml centrifuge tube ...
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bioRxiv - Microbiology 2023Quote: ... The aqueous phase was removed and transferred to MaxTract High Density 2 mL tubes (Qiagen Inc, Valencia, CA, USA). Samples were then extracted a second time as described above and the aqueous phase from the repeated extractions for each sample were combined ...
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bioRxiv - Microbiology 2022Quote: ... 1 to 2 mL of culture were mixed with the same volume RNAprotect™ Bacteria Reagent (Qiagen, Hilden, Germany) incubated for 10 min and centrifuged ...
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bioRxiv - Immunology 2022Quote: His-tagged RBD of SARS-CoV S was diluted to a concentration of 0.8 μg/mL in Tris-buffered saline (TBS) and immobilized on NiNTA 96-well plates (Qiagen) for 2h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... cfDNA was extracted from 0.5 ml of plasma or serum using the QIAmp DNA Mini Blood kit (Qiagen, CA), according to the ‘‘Blood and body fluid protocol’’ and our own detailed protocol [37] ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble fraction was recovered by centrifugation at 40 000 × g in a JLA25.50 rotor and put through a gravity flow column with 5 ml of NiNTA Agarose (Qiagen). Bound fraction was washed in Buffer B (300 mM NaCl ...