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Citations for Qiagen :
951 - 1000 of 2787 citations for 1 tert Butoxy 4 methyl 2 nitrobenzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Primers for Jamaican fruit bat genes (Supplemental Table 2) and qPCR was performed (QuantiTech SYBR Green, Qiagen). Within sample gene normalization was performed on Rps18 (ΔCq) ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen) in a gravity flow column and then washed with 300 mL of lysis buffer D ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was then performed in a 20 μL reaction volume with 2×SYBR Green qPCR mix (Qiagen), 100 nM each of the sense and antisense primers (Table 1) ...
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bioRxiv - Physiology 2022Quote: ... Fifteen milligrams of liver were homogenized in 200 µL of a 3:3:2 solution of acetonitrile:isopropanol:water (MeCN:IPA:H2O) in ceramic bead tubes (1.4 mm, Qiagen #13113-50) using a TissueLyzer II (Qiagen #85300) ...
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bioRxiv - Pathology 2022Quote: ... HSP90AB1) and fold changes/regulation of gene expression were calculated using the 2^(−ΔΔCT) formula (GeneGlobe, QIAGEN). Differential expression (up and down regulation ...
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bioRxiv - Cell Biology 2022Quote: Amplicons were separated on 2% agarose-TAE gels and purified using the QIAquick Gel Extraction Kit (Qiagen). Amplicons were sequenced at Quintarabio ...
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bioRxiv - Microbiology 2024Quote: ... 2 g of soil were used for RNA extraction using the RNeasy PowerSoil Kit (Qiagen, Venlo, Netherlands) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from 2-5 million flash frozen cells using the RNeasy plus mini kit (Qiagen). Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New Englands Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was collected and incubated with 2 mL of Ni-NTA Superflow affinity resin (Qiagen), previously equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from pools of 2-3 organoids using RNeasy Plus Mini Kit (Qiagen, #74134) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2022Quote: ... first the filter-harvested cells were resuspended in 2 mL RNAprotect Bacterial Reagent (Qiagen Catalog no. 76506), then pelleted in a centrifuge ...
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bioRxiv - Immunology 2022Quote: ... the frozen lung specimens were weighed before homogenization in PBS/2% FBS solution using the TissueLyser (Qiagen), as previously described (Tseng et al. ...
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bioRxiv - Genomics 2022Quote: ... Samples were incubated overnight at 55°C before adding 2 µl of RNaseA (100 mg/ml, Qiagen) and incubated for 15 min at 45°C ...
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bioRxiv - Neuroscience 2023Quote: Total mRNA was extracted from the PBMC obtained from 2 alpacas using standard RNA extraction kit (Qiagen). Recovered mRNA was retrotranscribed to cDNA by using Supercript IV (ThermoFischer ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from 2 biological replicates of fecal pellets using DNeasy PowerLyzer PowerSoil kit (Qiagen). Concentration of Pc and Bt DNA was assessed using species-specific qPCR primers (Key Resources Table) ...
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bioRxiv - Genetics 2024Quote: ... the final pool was purified from a 2% agarose gel (QIAEX II Gel Extraction Kit, Qiagen #20021).
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bioRxiv - Immunology 2024Quote: mTEC subpopulations were sorted and lysed in 20µl Lysis Buffer (0.02% 2-ME in 2x TCL (Qiagen)) using BD FACSAria TM III cell sorter (BD) ...
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bioRxiv - Microbiology 2024Quote: ... then homogenised with a steel ball for 2 minutes at 25 Hz using a Tissue-Lyser (Qiagen). CFU was quantified by plating to MacConkey agar containing 50 ug / ml streptomycin.
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted at 2 or 8 hpi with DNeasy Blood/Tissue DNA mini kit (Qiagen) and quantitated by a nanodrop spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... Each extraction step was carried out for 2 h in a TissueLyser II (Qiagen AB, Sollentuna, Sweden) at 6 s-1 at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged (2 minutes 12,000 RPM) before DNA-containing supernatant (2µL) was added to PCR master mix (18µL, Qiagen 201203 ...
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bioRxiv - Bioengineering 2024Quote: ... lysis buffer and homogenized at 1000 rpm for 2 min with 5 mm Stainless Steel Beads (Qiagen) using 1600 MiniG Tissue Homogenizer (SPEX SamplePrep) ...
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bioRxiv - Bioengineering 2024Quote: ... plasmids were purified from the 2-mL culture and eluted using 50 µL EB buffer from QIAGEN. The concentration usually was 50 ng/µL ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 hours at 37°C and subsequently purified using the QIAquick PCR Purification Kit (Qiagen, 28106). The modified lentiGuide-puro vector (Addgene Plasmid #52963 ...
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bioRxiv - Cell Biology 2024Quote: ... with 2-mercaptoethanol and stored at -80 °C until purification with a RNeasy Plus Micro Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... each mock community (Supplementary Table 2) was extracted with EZ1 DNA Tissue Kit (Qiagen; 953034; EZ1 method) and a separate aliquot with TRI Reagent® (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
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bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
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bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
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bioRxiv - Biophysics 2021Quote: ... The capture probes contained an 11- to 12-nt target-capturing sequence with 4 LNA residues (Tm = ~70°C, estimated using Qiagen web application) for high affinity and kinetically stable capturing of nucleic acid targets on the surface ...
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bioRxiv - Genomics 2020Quote: RNA was prepared from the 4-dpi flash-frozen spleen tissues of each of the above 20 chicks after homogenization in Qiazol reagent (Qiagen, Manchester, UK), and subsequent preparation using RNeasy RNA isolation kit (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from TG and NTG mice (n=4/group) at 6 months of age using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from whole fecal pellets or 50 μL of culture with a DNeasy PowerSoil HTP 96 kit (Qiagen 12955-4). 16S rRNA amplicons were generated using Earth Microbiome Project-recommended 515F/806R primer pairs using the 5PRIME HotMasterMix (Quantabio 2200410 ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from 1g of soil from 4 different samples from each sampled plot using the DNeasy PowerSoil Kit (Qiagen, Antwerp, Belgium) according with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 4 nmole standard desalted Ultramer™ DNA oligo from IDT) and PCR products (purified using QIAquick® PCR Purification Kit from QIAGEN) served as donor templates for small (3xFLAG epitope tag ...
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bioRxiv - Cancer Biology 2022Quote: ... 1,000 T-cells/CAR T-cells were FACS-sorted in single PCR tubes containing 4 ul of QIAGEN lysis buffer from QIAseq FX Single Cell RNA Library Kit (QIAGEN, Hilden, Germany) and stored at −80°C ...
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bioRxiv - Microbiology 2022Quote: Isolation of total DNA from a total of 107 transfected MOLT-4 cells was done using DNeasy Blood & Tissue Kit (Qiagen catalog # 69504). Isolated DNA was digested with either NcoI-HF (New England Biolabs # R0193 ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was applied to a nickel-NTA affinity column containing a total of 4 ml bed volume of nickel-NTA superflow resin (Qiagen, Venlo, Netherlands). After washing with a washing buffer containing 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... spun down (400 g × 10’ at 4 °C) and the pellet was resuspended in 600μ of Qiazol Lysis Reagent (Qiagen, Cat.No. 79306) and kept at −80 °C before sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... step for 15 min at 4°C to remove insolubilized material and the supernatant was incubated with Ni-NTA agarose beads (Qiagen, cat: 30230) for 2 hrs ...
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bioRxiv - Genetics 2020Quote: ... DNA binding plate was incubated with 600 μL of liquid for 4 minutes at RT and centrifuged at 1,300 g for 5 minutes sequentially with PB buffer (Qiagen Cat. No. 19066), followed by PE buffer (Qiagen Cat ...
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bioRxiv - Physiology 2020Quote: ... Sections were then post-fixed in 4% PFA/PBS before washing with buffer containing Proteinase K (PK; Qiagen UK Ltd, Manchester, UK) (1 µg/ml PK ...
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bioRxiv - Cancer Biology 2021Quote: RNA was isolated from HFC (n = 3) and LFC (n = 4) tumor samples using the RNeasy Plus Universal Mini Kit (QIAGEN, Valencia, CA) and RNA quality was confirmed using an Advanced Analytical Fragment Analyzer ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was cleared by centrifugation at 18000 rpm at 4°C and the supernatant was loaded onto 5 mL Ni–NTA resin (Qiagen, Milan, Italy) equilibrated with binding buffer ...
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bioRxiv - Immunology 2021Quote: ... Samples were centrifuged at 14,000 g at 4°C for 10 mins and the supernatant collected for processing using a RNeasy mini-isolation kit (Qiagen Ltd, UK) according to the manufacturers’ protocol ...
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bioRxiv - Immunology 2020Quote: ... Vγ4+TCRγδ+CD3+CD44+CD27− and Vγ4−TCRγδ+CD3+CD44+CD27− populations from each mouse strain were sorted using a FACSAriaIII: populations into RNA protect (QIAGEN, Hilden, Germany). Total RNA was extracted from the sorted cells according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN) ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... striatal and retinal RNA from the same R6/1 mice was pooled (3-4 samples per genotype) and further processed using the clean-up protocol of the RNeasy Mini Kit (Qiagen, Hilden, Germany), which also included on-column DNase I treatment ...
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bioRxiv - Plant Biology 2023Quote: Total RNA were extracted from the basal stem section of mature Col-0 and msil2/4 mutant plants using the RNeasy Plant Mini Kit (Qiagen, Cat.#74904) and treated onto the column with the RNase-Free DNase Set (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 20,000 x g for 45 minutes at 4°C and incubated with Ni-NTA agarose resin (Qiagen, catalogue number: 30230) pre-equilibrated with lysis buffer for 2 hours at 4°C ...