Labshake search
Citations for Qiagen :
51 - 70 of 70 citations for u 78057 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... 100 μg of the isolated total RNA was treated with 30 U of DNase I (QIAGEN) and purified using RNeasy Mini Kit (QIAGEN) ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We removed remnant RNA by incubating the DNA with 14 U of RNase A (Qiagen, Germany) at room temperature for 4 min and inactivated the RNase by freezing at −20 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 20 μl containing Hot Start Taq (1 U, QIAGEN), 3.5 mM MgCl2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
-
bioRxiv - Immunology 2020Quote: ... 2 μL of cDNA was added to 19 μl PCR reaction mix so that the final reaction contained 0.2 μL (0.5 U) HotStarTaq Polymerase (Qiagen), 0.075 μL of 50 μM 3′ reverse primers ...
-
bioRxiv - Zoology 2020Quote: ... Two independent replicates were performed in 8 μl of volume containing 0.5 U of Hot Start Taq polymerase (Qiagen), 0.18 μM of each primer and 0.04 mg of Bovine Serum Albumin Fraction V (Roche) ...
-
bioRxiv - Genomics 2020Quote: ... first strand synthesis was performed by incubating the pucks in 200 μL of reverse transcription solution (Maxima 1x RT Buffer, 1 mM dNTPs, 2 U/μL Lucigen NxGen RNAse inhibitor, 2.5 μM template switch oligo with Qiagen #339414YCO0076714 ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1 µl NEB2 buffer and 0.1 U enzyme were incubated 15 min 30°C and purified from unincorporated nucleotides using Nucleotide Removal Kit (Qiagen). Binding reactions were carried out in a total volume of 10 µl ...
-
bioRxiv - Immunology 2022Quote: ... 2 μL of cDNA was added to 19 μL first round PCR reaction mix containing 0.5 U HotStarTaq Polymerase (Qiagen), 0.18 μM of each 3’ reverse primer ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2020Quote: ... Total RNA (2000 ng) was transcribed into cDNA in a volume of 20 μl using 4 U Omniscript reverse transcriptase (Qiagen). To determine the relative abundance of the mRNAs of interest ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were performed in a final volume of 50 µl using 2.5 U of HotStar HiFidelity DNA polymerase (Qiagen,Germany), 2.5 µl of 10 µM primers ...
-
bioRxiv - Microbiology 2022Quote: ... 2 µL of cDNA was added to 19 µl PCR reaction mix so that the final reaction contained 0.2 µL (0.5 U) HotStarTaq Polymerase (Qiagen, cat#203607), 0.075 µL of 50 µM 3′ reverse primers ...
-
bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... Total RNA from mock-treated or IFNα-treated cell lines (1000 U, 24 hour treatment) was extracted using an RNeasy Plus Mini Kit (Qiagen) as indicated in the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... and 20 U/mL RNasin) and resuspended in 100 µL of RLT buffer from the RNeasy Micro Kit (Qiagen 74004) supplemented with 1% β-mercaptoethanol ...
-
bioRxiv - Molecular Biology 2024Quote: ... The primer extension reaction was performed in a volume of 20 μL with a 32P-labeled primer (complementary to galM of the galactose operon mRNA; Table S1) and 1 U of Taq polymerase (Qiagen) (X et al ...
-
bioRxiv - Molecular Biology 2024Quote: ... was reverse transcribed at 37 °C for 2 h in a 20 μL reaction volume containing 4 U of Omniscript reverse transcriptase (Qiagen), 0.5 mM each dNTP ...
-
bioRxiv - Cell Biology 2022Quote: ... diluted 2-times and 1 μl was taken for each PCR reaction (20 ul) containing: 0.02 U HotStarTaq® DNA polymerase (Qiagen, Germany), 1 x PCR buffer ...
-
bioRxiv - Neuroscience 2023Quote: ... and 20 U/ml RNasin just before use) and resuspended in 100 μl RLT Buffer from the RNeasy Micro Kit (Qiagen #74004) supplemented with 1% β-mercaptoethanol ...