Labshake search
Citations for Qiagen :
51 - 100 of 1550 citations for Tubulin inhibitor 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... Inhibitor removal and sample clean-up is performed with Solution C2 and C3 (Qiagen). The supernatant and pellet fractions are recombined and DNA purification performed according to the manual protocol of the NucliSENS Magnetic Extraction Reagents kit (BioMérieux).
-
bioRxiv - Molecular Biology 2020Quote: ... and complete mini EDTA free protease inhibitor followed by mechanical homogenation by Tissue Ruptor (Qiagen). The samples were centrifuged at 13000 rpm for 15 mins and protein concentration calculated by BCA ...
-
bioRxiv - Molecular Biology 2022Quote: ... 0.1 % SDS) containing protease inhibitors and 0.1mM of 1,10- phenanthroline using a TissueLyser II (QIAGEN). The samples were then quantified ...
-
bioRxiv - Molecular Biology 2021Quote: ... commercially obtained hsa-let-7a-5p or hsa-miR-205-5p miRCURY LNA miRNA Inhibitor (Qiagen, cat# YI04101776 and YI04101508 ...
-
bioRxiv - Molecular Biology 2021Quote: ... miRCURY LNATM miRNA inhibitor for anti-miR20a-5p and anti-miR149-5p were bought from Qiagen. Total RNAs were extracted 24 hours or 96 hours (for anti-miR test ...
-
bioRxiv - Microbiology 2019Quote: ... protease and phosphatase inhibitors) or RLT buffer supplemented with “- mercaptoethanol (RNeasy mini kit, Qiagen, Hilden, Germany) for western blot analysis and total RNA extraction ...
-
bioRxiv - Microbiology 2020Quote: ... library preparation was repeated after DNA inhibitor removal using the DNEasy PowerClean Pro Cleanup Kit (Qiagen). 50 ng DNA was used to prepare the library ...
-
bioRxiv - Genomics 2022Quote: ... DE cells were treated with 500 nM LNA miR-182 inhibitor (Qiagen, Hilden, Germany; #4101243-101) or scrambled control (Power Negative Control A ...
-
bioRxiv - Neuroscience 2022Quote: ... miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P, Qiagen #339130 YI04101229-DCA).
-
bioRxiv - Neuroscience 2022Quote: ... miR-329 pLNA (miRCURY LNA miRNA Power Inhibitor RNO-MIR-329-3P, Qiagen # 339130 YI04101481-DCA), miR-495 pLNA (miRCURY LNA miRNA Power Inhibitor HSA-MIR-495-3P ...
-
bioRxiv - Neuroscience 2022Quote: pLNAs used were: Control pLNA (miRCURY LNA miRNA Power Inhibitor Negative control A, Qiagen #339135 YI00199006-DCA), miR-329 pLNA (miRCURY LNA miRNA Power Inhibitor RNO-MIR-329-3P ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.5% deoxycholic acid with a protease inhibitor) and pre-cooled tissue homogenizer (TissueLyser LT, Qiagen GmbH, Hilden, Germany) for 2-4 min at 45 Hz ...
-
bioRxiv - Microbiology 2022Quote: ... Synthetic miRNA mimics (miRCURY LNA miRNA mimics) and LNAs (miRCURY LNA miRNA power inhibitors) were obtained from QIAGEN. Synthetic antagomir were obtained from Ribobio ...
-
bioRxiv - Genetics 2022Quote: ... DNA extracts were then purified to remove environmental inhibitors using the DNeasy PowerClean Pro Cleanup Kit (Qiagen®), as per the usual procedure of eDNA water sample analysis (Villacorta-Rath et al. ...
-
bioRxiv - Molecular Biology 2023Quote: Inhibitors (antimiRs) against miR26b-5p and miR200a/b/c-3p were purchased from Qiagen (339130 and 339160 respectively). AntimiRs in injection media (1mM Tris-HCL pH 7.5 and 0.5 mM EDTA in embryo grade water ...
-
bioRxiv - Developmental Biology 2022Quote: ... we treated rat AFSCs at 60% confluence with miR-17-5p and - 20a power inhibitors (25 μM) following the manufacturer’s protocol (Qiagen YI04100215-DDA ...
-
bioRxiv - Cancer Biology 2022Quote: ... Tissue was homogenized in 150uL of RIPA buffer with protease and phosphatase inhibitors by bead homogenizer (Tissue LyserII, Qiagen) for 3 mins at a frequency of 30 hz/s and immediately cooled for 5 mins on ice ...
-
bioRxiv - Pathology 2020Quote: 200 microgram of kidney tissue were homogenized and lysed by 8M urea in 20mM HEPES (pH=8.0) buffer with protease and phosphatase inhibitors using Tissue Lyser II (QIAGEN). Samples were reduced and alkylated followed by MS-grade trypsin digestion ...
-
bioRxiv - Physiology 2021Quote: Lyophilized mouse hearts were homogenized in RIPA buffer supplemented with protease and phosphatase inhibitors (Pierce) using a TissueLyser II apparatus (Qiagen), followed by sonication and centrifugation (10 min at 12,000 rpm at 4°C) ...
-
bioRxiv - Immunology 2019Quote: ... SLE mice were retro-orbitally injected with 10 mg/kg of LNA-miR-22-3p (LNA-22) or scrambled control (LNA-Scr) (miRCURY LNA Inhibitor probe in-vivo, Qiagen) every 2 weeks beginning at 10-12 weeks of age ...
-
bioRxiv - Cell Biology 2021Quote: ... Muscle samples were next lysed in ice-cold radioimmunoprecipitation assay (RIPA) buffer (400 μL/muscle) with protease and phosphatase inhibitor cocktail (Cell Signalling) using TissueLyser II (QIAGEN). Half of the lysate was mixed with scintillation cocktail for scintillation counting using Liquid Scintillation Analyzer (PerkinElmer) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells incubated for 24 hours in a 37 °C incubator and transfected with hsa-miR-24-3p miRCURY LNA miRNA Power Inhibitor (Qiagen, Germantown ...
-
bioRxiv - Immunology 2020Quote: ... 50 mM Tris-HCl pH 8.0) with 0.1 μL Protease Inhibitor Cocktail VI (Astral Scientific No. P-1515) using the TissueLyser II (Qiagen No. 85300) at 25 Hz for 2 min twice ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Brain and DRG samples from up to four donors per species were homogenized in Tissue Protein Extraction Reagent (T-PER) supplemented with HALT protease inhibitor and benzonase endonuclease using a Tissue Lyser Mixer Mill Grinder (Qiagen), while serum and CSF were directly diluted in supplemented T-PER ...
-
bioRxiv - Genomics 2019Quote: Total RNA was isolated from Arabidopsis seedlings grown for 10 days and exposed to DMSO or splicing inhibitors for 6 or 24 hours with RNeasy Plant Mini Kit (Qiagen) according to manufacturers’ instructions ...
-
bioRxiv - Pathology 2023Quote: ... tissue and cells were lysed in RIPA lysis buffer supplemented with orthovanadate and a cocktail of protease inhibitors at 4°C (using a TissueLyser (Qiagen) to homogenize liver tissue ...
-
bioRxiv - Neuroscience 2024Quote: ... with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and TissueRuptor II (Qiagen) homogenization ...
-
bioRxiv - Neuroscience 2024Quote: ... 100 mg of frozen brain was taken per mouse sample and lysed with 0.9 x tissue mass of lysis buffer (1x RIPA buffer with 2% SDS and 2x protease inhibitor cocktail) and was homogenised using a TissueRuptor II (Qiagen). Zebrafish larvae were similarly extracted ...
-
bioRxiv - Neuroscience 2023Quote: ... primary hippocampal neurons were transfected in duplicates or triplicates using 150ng of a plasmid carrying enhanced GFP-gene in combination with 5nmol Power Lock-Nucleic Acid inhibitors (Qiagen) against miR-218-5p ...
-
bioRxiv - Genomics 2019Quote: KARPAS-422 cells treated with DMSO or EZH2 inhibitor were harvested and total RNA was isolated with Qiagen RNeasy Plus Mini kit (Qiagen 74136). Downstream sample processing ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were centrifuged for 5 minutes at 300g and resuspended in 1mL PBS + RNase Inhibitors (Invitrogen AM2694, Qiagen Beverly Inc Y9240L) and fixed as previously described for split-pool ligation-based RNAseq ...
-
bioRxiv - Genetics 2020Quote: ... 1 µl 1 mg/ml Carrier RNA (QIAGEN), 1 µl 10% SDS ...
-
bioRxiv - Neuroscience 2021Quote: ... Hsa-miR-124-3p Locked Nucleic Acid (LNA) power inhibitor and has-miR-124- 3p miRCURY LNA miRNA mimic (Qiagen, Germantown, MD) were resuspended with RNase-free water to 100 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... protease and phosphatase inhibitors (PMSF, Aprotinin, Pepstatin, Leupeptin) and homogenised with autoclaved metal beads in a TissueLyser II instrument (Qiagen, Hilden, Germany), followed by ultrasonication (8 sec ...
-
bioRxiv - Genomics 2020Quote: ... 1 mM EDTA and 1% freshly added Proteinase K (Qiagen)) was added to the cell suspension ...
-
bioRxiv - Cell Biology 2019Quote: ... CLASP-1 (Qiagen) – GCCAUUAUGCCAACUAUCUdTdT (Mimori-Kiyosue et al. ...
-
bioRxiv - Cell Biology 2019Quote: ... Kid-1 (Qiagen) – CAAGCUCACUCGCCUAUUGTT (Wandke et al. ...
-
bioRxiv - Cell Biology 2021Quote: ... DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup ...
-
bioRxiv - Developmental Biology 2022Quote: ... SPACA6 concentrated to 7 mg mL−1 in Buffer B was mixed in a 1:1 volumetric ratio (0.3:0.3 mL) with JCSG+ (Qiagen), Cryos (Qiagen) ...
-
bioRxiv - Genomics 2019Quote: ... and 1 μL of 1 AU/mL protease (Qiagen cat # 19157) to each well followed by a 60 min incubation at 55 °C ...
-
bioRxiv - Biophysics 2020Quote: ... or DNAse (200 Units well−1, 1 hr, 37°C, Qiagen). DAPI was stained at 0.5 μM (5 mins ...
-
bioRxiv - Immunology 2022Quote: ... Virus was inactivated by mixing 1:1 with Buffer ATL (Qiagen) prior to viral RNA extraction from NP swabs ...
-
bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected with 1:1:2 μg of packaging plasmids versus shRNA hairpins on the pLKO.1 vector using Effectene transfection reagent (Qiagen) 48 h prior to harvesting supernatants ...
-
bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 μg DNA ratio using Effectene transfection reagent (Qiagen). Recombinant luciferase-expressing viruses capable of a single round of replication were released into the cell medium and were harvested 48 h later ...
-
bioRxiv - Microbiology 2023Quote: ... the pCMVΔP1Δenv HIV-1 Gag-Pol packaging construct and the firefly luciferase-expressing HIV-1 vector at a 1:1:3 µg DNA ratio using effectene transfection reagent (Qiagen). Recombinant ...
-
bioRxiv - Microbiology 2023Quote: ... 5.0 x105 293T cells were co-transfected with HIV-1 Env-expressing and HIV-1 Tat-expressing plasmids at a ratio of 1:6 using Effectene (Qiagen) and incubated for 48 hours ...
-
bioRxiv - Genomics 2020Quote: ... 1 ul (1U/ul) UDG and 1 ul RNaseA (100 mg/ml, Qiagen) and reactions were incubated at 37°C for 3 hrs ...
-
bioRxiv - Genomics 2023Quote: ... gracilis (1 male and 1 female) using the Rneasy Mini Plant Kit (Qiagen) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... purified nucleic acids were treated with 1-5 μl 1 mg/ml RNaseA (Qiagen) for 45 minutes at 37 °C to degrade RNA ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 30 uL/well 1 X lysis buffer (1x Qiagen TCL, 1% beta-mercaptoethanol) was added ...