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Citations for Qiagen :
51 - 100 of 279 citations for Eukaryotic Peptide Chain Release Factor GTP Binding Subunit ERF3A GSPT1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Recombinant protein was purified by binding to Ni-NTA beads (Qiagen) and eluted in the same buffer supplemented with 300 mM imidazole ...
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bioRxiv - Physiology 2020Quote: ... The polymerase chain reaction (PCR) were carried out using Rotor Gene Q (Qiagen, Hilden, Germany) with initial incubation at 95 °C for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction was conducted using miRCURY LNA miRNA SYBR PCR kit (Qiagen #339345) as per manufacturer’s instruction on BioRad CFX96™ System in 96-well plates in 3-6 repeats ...
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bioRxiv - Molecular Biology 2021Quote: Polymerase chain reaction (PCR) products were gel purified using Qiagen QlAquick gel purification columns (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Networks involving miRNAs and mRNAs were designed with the Path Designer tool from IPA (Winter Release December 2023, QIAGEN, Aarhus, Denmark).
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bioRxiv - Molecular Biology 2020Quote: ... After 14 h binding to 4 mL Ni-NTA agarose beads (Qiagen), the beads were first washed with 15 mL Ni-NTA binding buffer and then with 15 mL Ni-NTA wash buffer (1 M NaCl ...
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bioRxiv - Immunology 2021Quote: ... 30 μl Ni2+ beads equilibrated in binding buffer (Qiagen Ni-NTA superflow) was added ...
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bioRxiv - Immunology 2020Quote: ... had positive Interferon Gamma Release Assay (IGRA) (performed by the local hospital laboratories, using the QuantiFERON-TB Gold In-tube assay [Qiagen, Manchester, UK]), with known exposure to an index person with isoniazid and rifampicin susceptible pulmonary TB (unconfirmed for three individuals ...
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bioRxiv - Developmental Biology 2024Quote: The list of differentially expressed genes for various conditions was imported into Ingenuity Pathway Analysis (IPA, Fall 2023 release version, QIAGEN Digital Insights) to analyze upregulated and downregulated biological pathways ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time polymerase chain reactions were performed using the QuantiFast SYBR Green PCR Kit (QIAGEN) and the CFX ConnectTM Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Neuroscience 2022Quote: ... Transgenic animals were identified by polymerase chain reaction of tail DNA (DNeasy kit, Qiagen, Hilden, Germany) using primers for Cre (Schönig et al. ...
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bioRxiv - Cell Biology 2022Quote: ... in a Rotor-Gene® Q real-time polymerase chain reaction machine (Qiagen Inc., Germantown, MD). The PCR primers were designed with Primer Premier 6 (Premier Biosoft Int ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of recipient females using standard protocols (Qiagen DNeasy 96 Blood & Tissue kit). For each sample ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction was performed using miRCURY LNA SYBR Green PCR Kit (Qiagen) following recommended instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time polymerase chain reactions were performed on a Corbett Rotor-Gene 6000 Detection System (Qiagen) using a QuantiTect SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... Digital polymerase chain reaction (dPCR) was performed using the QIAcuity Digital PCR System (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... along with the human NaV β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.X:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transiently co-transfected with NaV1.2 and the accessory β1 and β2 subunits (2 μg total plasmid DNA was transfected with a cDNA ratio of 10:1:1 for NaV1.2:β1:β2 subunits) using Qiagen SuperFect reagent (Qiagen, Valencia, CA, U.S.A.). Human β1 and β2 cDNAs were cloned into plasmids encoding the CD8 receptor (CD8-IRES-hβ1 ...
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bioRxiv - Biophysics 2021Quote: ... Supernatant was collected for 2.5 h binding with Ni-nitrilotriacetic acid resins (Qiagen) at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... according to the manufacturers’ instructions and purified through a DNA-binding column (Qiagen). The quality and quantity of cDNA was measured using a Nanodrop spectrophotometer (Nanodrop Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting supernatant was subject to binding with Ni-NTA agarose resin (Qiagen) for 30 min at 4 °C with gentle rotation and loaded into the glass chromatography columns (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Bead binding was performed by adding washed beads (Ni-NTA agarose beads (Qiagen) for His-tagged proteins and GSH agarose beads (ABT ...
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bioRxiv - Neuroscience 2020Quote: ... Extracted DNA was used for genotyping by polymerase chain reaction (PCR) (Taq DNA polymerase, Qiagen, Hilden, Germay), using primers flanking the endogenous CAG repeat in exon 10 of the murine Atxn3 gene (primer sequences ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was amplified by polymerase chain reaction (PCR) with QuantiTect Sybr Green PCR Kit (Qiagen, Germantown, MD) on an Applied Biosystems QuantStudio 7 Flex real-time quantitative PCR instrument ...
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bioRxiv - Cancer Biology 2022Quote: A ready-to-transduce transcription factor-responsive lentiviral reporter system (CCS-1022L, QIAGEN) was used to generate a stable cell line ...
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bioRxiv - Cell Biology 2022Quote: ... His-EHD1 was purified by binding with Ni2+-NTA His-bind slurry (Qiagen, Germany) and eluting with imidazole as described previously (65) ...
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bioRxiv - Bioengineering 2022Quote: ... 0.01M Tris-Cl (pH 8.0) and passed through a Ni-NTA binding column (Qiagen). The loaded column was washed with 8M urea ...
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bioRxiv - Biochemistry 2022Quote: ... The detection of binding was carried out by the RGS His6 HRP conjugate (QIAGEN) that detects the RGS His6 motif present in the affitins N-terminal ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Functional enrichment analysis of genes proximal to African taurine and Asian zebu local ancestry peaks was performed using Ingenuity® Pathway Analysis—IPA® (version 1.1, summer 2021 release; Qiagen, Redwood City, CA, USA) (Kramer et al ...
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Novel RNA-binding protein YebC enhances translation of proline-rich amino acid stretches in bacteriabioRxiv - Molecular Biology 2024Quote: ... The RNA-peptide conjugates were purified with RNeasy Midi kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... or the QuantiTect PCR Kit with self-designed primers for neuronal factors (Qiagen, Germany) (Supplementary Table 3) ...
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bioRxiv - Cell Biology 2022Quote: ... The mRNA binding to beads was eluted and purified with RNeasy MinElute Spin columns (Qiagen), followed by cDNA synthesis ...
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bioRxiv - Genomics 2023Quote: ... Reactions were stopped by adding the appropriate volume of Binding Buffer (Qiagen MinElute PCR Kit) and the DNA was purified using the Qiagen MinElute PCR Kit according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer) to digestive mixture ...
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bioRxiv - Microbiology 2024Quote: ... and resuspended in 120 µl of water and 600 µl of binding buffer PB (Qiagen). GBS cell wall was lysed by bead beating (0.1 µM beads ...
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bioRxiv - Immunology 2021Quote: ... was then added to a polymerase chain reaction (PCR) mix containing 12.5 μl of 2× Multiplex PCR mix (Qiagen), 9 μl of H2O ...
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bioRxiv - Microbiology 2020Quote: ... Insertion of the dcas9/lacI cassette at the desired chromosomal location in strain CJW_Bb362 was confirmed by polymerase-chain reaction (PCR) analysis of isolated total genomic DNA (DNeasy Blood & Tissue Kit, Qiagen), using the following primer pairs ...
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bioRxiv - Genetics 2020Quote: ... Multiplex polymerase chain reaction (PCR) was performed using three primer pairs and the Multiplex PCR Kit (QIAGEN, Hilden, Germany). Each reaction contained 2× QIAGEN multiplex PCR master mix ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments that were generated by the polymerase chain reaction (PCR) were purified using the PCR Purification Kit (Qiagen), Monarch® PCR & DNA Cleanup Kit (NEB) ...
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bioRxiv - Immunology 2024Quote: ... Full-length heavy chain sequences were amplified using equimolar VH and JH primer cocktails and Multiplex PCR Kits (Qiagen), as previously described (Rosenfeld et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out using a QuantiFast SYBR Green PCR kit (QIAGEN, 204056) on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Genetics 2021Quote: Relative telomere length of MDS patients was measured by quantitative polymerase chain reaction (qPCR) as previously reported.8 K562 cell DNA was extracted using the QIAamp Blood Mini kit (Qiagen). Telomere length was measured by two orthogonal methods ...
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bioRxiv - Microbiology 2020Quote: ... One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) was then performed on extracted RNA samples using a QuantiTect Probe RT-PCR kit (Qiagen) following the standard protocol for a QuantStudio 7 Flex RT-PCR system ...
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bioRxiv - Biochemistry 2020Quote: ... low melting point agarose gel and the approximately 700 base pair band corresponding to the heavy chain only immunoglobulin was extracted and purified using QIAquick Gel Extraction Kit (Qiagen). A secondary amplification of the VHH domain was performed using modified primers (VHH-Esp-For ...
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bioRxiv - Microbiology 2020Quote: ... Gene expression was assessed using SYBR GreenER based-quantitative polymerase chain reaction (qPCR) or RT2 profiler arrays (Qiagen Ltd, UK). For SYBR GreenER based-qPCR analyses ...
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bioRxiv - Cell Biology 2022Quote: ... Drosophila S2 cells were transfected with mNeonGreen-tagged kinesin-1 full-length heavy chain (KHC) and Halo-tagged PLP584-1811 (Effectene, QIAGEN). The lysates were harvested for TIRF motility assays at ∼48 h post-transfection ...
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bioRxiv - Microbiology 2020Quote: Quantitative polymerase chain reaction (qPCR) analyses were performed on the new DNA extracts using PowerSoil DNA Isolation Kit (12888-50, QIAGEN) to estimate the abundance of bacteria and archaea at different depths in the sediment core ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgK PCR reaction was used for the 2nd round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... The same cDNA reaction was also used for the 1st round PCR of IgK chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... This 1st round IgG PCR reaction was used for the 2nd round PCR of IgG chains using a commercially available Hotstart DNA polymerase kit (Qiagen). The 10 μL reaction mixture consisted of 1X PCR buffer ...