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Citations for Qiagen :
51 - 100 of 3037 citations for AS 1517499 CAS 919486 40 1 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A 100 mg portion was taken from each fecal sample to extract genomic DNA using the DNeasy PowerSoil Pro Kit from Qiagen (Qiagen, Palo Alto, CA, USA). Subsequently ...
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bioRxiv - Pathology 2022Quote: ... RNA was isolated from maternal and fetal tissues (Table 1) using QIAamp cador pathogen mini kit (Qiagen, Valencia, CA). ZIKV RNA was quantitated by one-step quantitative real time reverse transcription PCR using QuantiTect probe RT-PCR kit (Qiagen ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was extracted from about 1 × 106 cells from all groups using RNeasy Mini Kit (Qiagen, Valencia, CA), then using the random hexamer primer and M-MuLV reverse transcriptase kit (Fermentas ...
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bioRxiv - Genomics 2024Quote: ... Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen, Valencia, CA) and kept on ice until use ...
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bioRxiv - Genetics 2021Quote: ... 40 out of 349 samples underwent DNA extraction using DNeasy Blood and Tissue Extraction Kit (Qiagen) and resulting DNA was subsequently genotyped on the Affymetrix 670K equine SNP array (Schaefer et al ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells were transfected with 40 nM specific human RBM10-siRNA5 or control AllStar siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were eluted in 40 ml of elution buffer (Qiagen, 10 mM Tris-Cl, pH 8.5) with optional 15 mins of incubation at 37°C to recover long molecules ...
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bioRxiv - Physiology 2024Quote: ... The 40 µl reaction mix consisted of 10 µl of 4x QIAcuity Probe PCR Kit (QIAGEN), 2 µl of the target assay ...
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bioRxiv - Biochemistry 2023Quote: ... cells were washed twice with 40 µL/well flow buffer and stained with streptavidin-PE (Qiagen) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... with SYBR Green (QIAGEN, Valencia, CA) as the detection method ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and RNeasy Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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A New Gene Set Identifies Senescent Cells and Predicts Senescence-Associated Pathways Across TissuesbioRxiv - Cell Biology 2021Quote: ... using SYBR green (Qiagen, Valencia, CA). The mouse forward primer sequence (5’-3’ ...
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bioRxiv - Plant Biology 2021Quote: ... PCR purification kit (Qiagen, Valencia, CA) was used for DNA purification ...
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bioRxiv - Physiology 2021Quote: ... using SYBR green (Qiagen, Valencia, CA). The mouse primer sequences ...
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bioRxiv - Pathology 2022Quote: ... The N gene-specific primers were used to amplify 97 bp of SARS-CoV-2 N gene by conventional PCR and purified by Qiagen gel extraction kit (Qiagen, CA, USA). The purified N gene PCR products were used in real-time PCR to prepare a standard curve and determine the viral copy numbers in the lung samples.
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bioRxiv - Immunology 2023Quote: ... AllPrep column purification (Qiagen, Valencia, CA) was used to isolate RNA ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA-free DNase (Qiagen, CA, USA) was added to the column to digest any DNA present in the sample ...
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bioRxiv - Physiology 2024Quote: ... using SYBR green (Qiagen, Valencia, CA). The mouse primer sequences ...
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bioRxiv - Physiology 2024Quote: ... with Trizol reagent (Qiagen, Carlsbad, CA) added to the resulting bone powder ...
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bioRxiv - Neuroscience 2024Quote: ... RNeasy Lipid Tissue kit (Qiagen, CA) was used with on-column DNase digestion as detailed in the kit user guide ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2021Quote: DNA extraction from 1 mL anaerobic digester sludge was performed using the DNeasy PowerSoil DNA extraction kit (Qiagen, Valencia, CA). DNA from Defo and Diel cultures was extracted from 5 mL culture suspensions collected onto 0.22 µm Durapore membrane filters (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were then analyzed on 1% agarose gel and purified using the QIAquick PCR Purification Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: Infected whole eyes were homogenized in 1 mL of sterile PBS using a Retsch Mixer Mill MM300 (Qiagen, Valencia, CA) at 33 Hz for 4 min ...
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bioRxiv - Biochemistry 2021Quote: ... rRNAs from pre-40S intermediates were phenol chloroform extracted and further cleaned using RNeasy Mini columns (Qiagen). 1ug of rRNA was used for each reverse transcription reaction using a primer that starts at 1241 of 18S rRNA (sequence listed in Table S4) ...
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bioRxiv - Microbiology 2021Quote: ... After amplicon purification with the QIAquick PCR purification kit (final elution 40 μL; Qiagen; Germantown, MD, USA), we used Sanger sequencing (Macrogen ...
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bioRxiv - Microbiology 2023Quote: ... followed by rigorous shaking for 40s at 5.5 m/s in a Tissuelyser II (Qiagen, Hilden, Germany). Serial dilutions of these samples were then plated on agar-solidified culture media (Fig ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from the 40 marine sediment samples using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germany) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... with 1 min rest on ice in between each round of bashing) on a TissueLyser II beadmill (Qiagen, Valencia, CA, USA), then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter ...
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bioRxiv - Genomics 2020Quote: ... total genomic DNA of FAFU-HL-1 was extracted using a Qiagen DNeasy Plant Mini kit following the manufacturer’s instructions (Qiagen, Valencia, CA). For ONT sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... the circular DNA (40 ng) was subjected to rolling circle amplification (RCA) using Repli-G midi kit (Qiagen). RCA is a technique used for multiple amplification of circular DNA to obtain sufficient amplicons for next-generation nucleotide sequencing (NGS ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthesized from 40 ng of isolated RNA using the RT2 First strand Kit (Cat#330404 Qiagen). Pre-Amplification of cDNA was done using Single cell to CT kit (Cat#445 8237 Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 30-40 mg of the sample were incubated in 100 μl of Extraction Buffer EXB Plus (Qiagen, Germany) containing 6% β-mercaptoethanol on ice for 5 min and mixed by vortexing ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.28 M sucrose, 40 mM Tris-Cl, pH 7.5, 20 mM MgCl2, and 4% Triton X-100; QIAGEN) and then digested by digestion buffer (QIAGEN buffer G2 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed (1.28 M sucrose, 40 mM Tris-HCl [pH 7.5], 20 mM MgCl2, and 4% Triton X-100; Qiagen) and digested (800 mM guanidine–HCl ...
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bioRxiv - Cell Biology 2023Quote: ... and 40 ng of cDNA was used for each qPCR reaction with QuantiTect SYBR green reagent (QIAGEN #204143). PCR primers for specific poly(A ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted from ∼40 mg of each sample using the DNeasy Plant Mini Kit (QIAGEN, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Cells were grown to 40% confluence and then incubated with 50 nM siRNA and Attractene transfection reagent (QIAGEN) for 36 h according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... HiPerFect reagent was from Qiagen (Valencia CA).
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bioRxiv - Molecular Biology 2021Quote: ... The Effectene transfection reagent (Qiagen, Valencia, CA) was used following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... placed into RNA later (Qiagen Valencia, CA) and frozen in liquid nitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and nickel affinity resin (Qiagen, Valencia, CA), respectively ...
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bioRxiv - Biophysics 2020Quote: ... and after plasmid amplification (QIAGEN, Valencia, CA). All constructs were in pcDNA3.1(+ ...
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bioRxiv - Microbiology 2022Quote: Endotoxin-free maxi preps (Qiagen, Valencia, CA) were prepared for all pRMΔ shuttle vectors as per manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: The RNeasy Mini Kit (Qiagen, Valencia, CA) and the SuperScript II cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and an RNasy column (Qiagen, Valencia, CA). Flies of four genotypes were used ...
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bioRxiv - Genomics 2020Quote: ... The RNeasy mini kit (Qiagen, CA, USA) was used according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... except a TissueLyser (Qiagen, Valencia, CA, USA) was used to disrupt mycelia and DNA was eluted into ultrapure water instead of AE buffer.