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Citations for Qiagen :
901 - 950 of 1346 citations for Mouse Anti Chikungunya Virus E2 Protein 18H01 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Total RNA was isolated from rat or mouse hippocampal cultures using the RNeasy Mini Kit (Qiagen, #74104). DNA contamination was removed by digestion with DNase I (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... and with a mouse antibody against the His-tag (Tetra·His Antibody, QIAGEN-Cat. No.34670, 1:2,000) in blocking solution on a rocker ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from the mouse lenses using the RNeasy Plus Micro Kit (QIAGEN, Venlo, Netherlands) per the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: 500ng mouse or 300ng sheep RNA was reverse transcribed using QuantiTect Reverse transcription kit (Qiagen, Manchester, UK) and gDNA wipe-out (to remove genomic DNA) ...
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bioRxiv - Physiology 2021Quote: ... Mouse C2C12 myotubes: Total RNA was extracted using TRIzol and purified using RNeasy Mini spin columns (QIAGEN). Reverse transcription was performed using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Neuroscience 2022Quote: RNA purification from both IPSC and primary mouse cortical cultures were done using RNeasy 96 Kit (Qiagen) according to the manufacturer protocol ...
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bioRxiv - Pathology 2022Quote: ... and total RNA of mouse mesenteric fat was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen). The isolated RNA was transcribed to complementary DNA via Superscript IV VILO Master Mix (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from transfected 293T cells and frozen mouse testes using RNeasy Mini kit (QIAGEN). Extracted RNA was used for cDNA synthesis using iScript cDNA Synthesis kit (BIO-RAD ...
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bioRxiv - Biochemistry 2023Quote: Genomic DNA (i.e., gDNA) was isolated from mouse liver tissue using the DNeasy Blood & Tissue Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from one quarter of a mouse kidney using RNeasy Plus Mini Kit (Qiagen, 74134). For quantitative real-time PCR ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from mouse cecal contents and in vitro cultures using the RNeasy PowerMicrobiome Kit (Qiagen). Ribosomal RNA depletion was performed using the RiboMinus™ Transcriptome Isolation Kit (Invitrogen) ...
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bioRxiv - Physiology 2024Quote: Total RNA from mouse hearts (normal, ischemic, or non-ischemic) was isolated using RNeasy Mini kits (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was done on mouse kidney lysate using the RNeasy Plus Universal Midi kit (Qiagen). RNase digestion was then performed on the RNA extract using the following RNases ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from de-identified tubes of mouse tissues using the RNeasy Kit (QIAGEN; Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and the pellet was lysed using RLT buffer (mouse cells) or QIAZol (human cells) (Qiagen, Cat. 79306). RNA was extracted using the Qiagen RNeasy micro kit.
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bioRxiv - Microbiology 2023Quote: Mouse cDNA was amplified with gene-specific primers (see below) and a HotStarTaq Master Mix Kit (Qiagen). Amplicons were cloned and inserted into a pGEM-T vector (Promega ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary hippocampal neurons grown on coverslips were transiently transfected at DIV11 with Alexa 488-labeled siRNA either specifically targeting mouse Kcc2b (Mm_Slc12a5_1 FlexiTube siRNA, GeneGlobe ID: SI01419243, GenBank accession no: NM_020333; Qiagen) or Ank3 mRNA (Mm_Ank3_3 FlexiTube siRNA ...
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bioRxiv - Cell Biology 2023Quote: ... and reads were aligned to the mouse reference genome Refseq GRCm39.105 from the Biomedical Genomics Analysis Plugin 20.0.1 (Qiagen). Normalization of RNA-seq data was performed using trimmed mean of M-values ...
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bioRxiv - Microbiology 2024Quote: Eukaryotic RNA was isolated from mouse tissue samples (stomach and colon) stored in RNAprotect Tissue Reagent (Qiagen) at ™70 °C before extraction using a GeneJET RNA Purification Kit (Cat ...
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bioRxiv - Genomics 2024Quote: RNA was extracted from mouse tissue samples using an AllPrep DNA/RNA Mini Kit (Qiagen, Valencia, CA). Homogenization buffer for RNA purification was made by adding 1:100 beta-mercaptoethanol to Buffer RLT (Qiagen ...
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bioRxiv - Biophysics 2020Quote: ... proteins were expressed in Escherichia coli strain BL21 (DE3) and purified via nickel-nitrilotriacetic acid affinity chromatography (Qiagen) followed by ion exchange chromatography on an Äkta system (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: Purified recombinant poly-histidine protein was obtained by FPLC under native conditions using a Ni-NTA column (Qiagen), and a Biologic DUO-FLOW BIO-RAD chromatography system (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... microdissected human brain tissue was flash frozen and lysed to extract proteins using a TissueLyser II system (QIAGEN) and total protein concentration determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific 23225) ...
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bioRxiv - Molecular Biology 2021Quote: ... The expressed EcoCas3-tag fusion proteins were purified using nickel affinity resin (Ni-NTA, Qiagen, Hilden, Düsseldorf, Germany). To remove tags ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells grown at 37°C by addition of 0.2 mM IPTG and native protein purified using Nickel affinity chromatography according to the manufacturer’s protocol (Qiagen). For N-terminal His-tag removal ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were purified from the cell lysate by affinity chromatography using a Nickel-NTA column (Qiagen, Valencia, CA). A solution containing 20mM HEPES pH7.2 ...
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bioRxiv - Biochemistry 2020Quote: Initial hits of Nup851-564-Seh1-VHH-SAN2 were obtained at 18 °C in 1 day in a 96-well sitting drop tray with a reservoir containing 1M ammonium sulfate and 0.1 M sodium acetate pH 5.0 (Protein Complex suite, Qiagen). Crystals significantly improved by changing the buffer to di-sodium succinate pH 5.5 and the addition of 4% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted using the Qiagen AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany; #80004). The standard manufacturer’s protocol was followed to extract DNA and RNA from each cell pellet ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was isolated from dissected tissues using an AllPrep DNA/RNA/Protein Mini kit (Qiagen, Valencia, CA). The concentration and purity were determined using a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then purified on Ni2+ -NTA affinity resin following a modified version of the manufacturer’s instructions (QIAGEN) [25] ...
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bioRxiv - Microbiology 2020Quote: ... The 6 × his-tagged proteins were purified using a nickel-nitrilotriacetic acid (Ni-NTA) agarose matrix (30250, Qiagen). Cell-free extracts were loaded on 0.5 ml Ni-NTA matrixes and incubated on a roller shaker for 2 h at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein lysates were separated on NuPage 5-12% Bis-Tris or 3-8% Tris-Acetate gels (Novex, Qiagen) using MES running buffer (Novex ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged proteins were purified by Ni-affinity gravity-flow chromatography using the Ni-NTA agarose (Qiagen) following the product manual.
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bioRxiv - Molecular Biology 2020Quote: ... His-tagged ubiquitin-conjugated proteins were purified by adding 100 µL of 50 % Ni-NTA agarose beads (Qiagen) equilibrated in A2 buffer to 1 mL of cleared extracts and incubated with rotation for 2-4 hr at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the tags and protease were removed from the protein by passage through a Ni-NTA superflow cartridge (QIAGEN). The protein was further purified by size exclusion chromatography on a Superdex 200 Increase (Cytiva ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His protein was purified from cell culture supernatant using a Ni-NTA (Qiagen) affinity column ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed on the eluted DNA samples at 50°C for 30 minutes (Qiagen Protease mix). ChIP DNA was purified using Sera-Mag beads (Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from the frozen placental tissue using the Protein and RNA Isolation (PARIS) kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The phage protein capsid was digested with Proteinase K and the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) was used to purify the DNA ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysates were clarified by centrifugation and proteins in the supernatant were purified using Ni-NTA chromatography (Qiagen) for His-tagged proteins or Glutathione Sepharose 4 Fast Flow beads (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The protein prokaryotic expression and purification was according to the instruction of Ni-NTA Agarose (Cat.No.30210, QIAGEN) and Glutathione Sepharose (GE).
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bioRxiv - Bioengineering 2020Quote: Initial small test purifications were performed using Ni-NTA Spin Column for His-Tagged proteins (Qiagen, Hilden, Germany) following the protocol for 6xHis-Tagged Proteins under Native Conditions from E ...
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bioRxiv - Biochemistry 2020Quote: ... The Ni-NTA agarose resin for purification of his-tagged proteins was purchased from Qiagen (Germantown, MD, USA). Superdex 75 16/600 and Superdex 200 Increase 10/300 size exclusion chromatography columns were purchased from GE Healthcare (Uppsala ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was isolated from 5 × 105 cells with the AllPrep© DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted from samples using the AllPrep DNA/RNA/Protein Mini Kit (80004, Qiagen Sciences, Inc.) and 0.25 μg of total RNA was reverse transcribed by SuperScript™ IV First-Strand cDNA Synthesis System (18091050 ...
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bioRxiv - Immunology 2021Quote: ... to provide Uniprot entries and further processed in Perseus (v1.6.6.1).18–20 Significantly regulated proteins were investigated in Ingenuity Pathway Analysis (v47547484, Qiagen, USA)
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bioRxiv - Developmental Biology 2021Quote: Log fold-change values of the top 5% proteins were used as input for Ingenuity Pathway Analysis (Qiagen: https://digitalinsights.qiagen.com/products/features/) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were disrupted by sonication and N-terminus His6-tagged proteins were purified on Ni-NTA columns (Qiagen) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins NHis6-Fnr1 and Fnr3-CHis6 in the supernatant were purified on Ni2-NTA resin (Qiagen, Germany) according to the manufacturer’s protocol ...