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Citations for Qiagen :
901 - 950 of 3601 citations for 6 Bromo N tert butyl 2 4 chlorophenyl imidazo 1 2 a pyridin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Button valves were opened and biotinylated anti-pentaHis antibody (Qiagen, 1:4 dilution in HEPES) was flowed for 30 minutes ...
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bioRxiv - Biophysics 2021Quote: ... and the bindingPCA plasmid containing the ligand GAB2 or CRIPT fused to DHFR1,2 (pGJJ006 and pGJJ066 respectively) were digested with the same enzymes and purified using the QIAquick Gel Extraction Kit (QIAGEN).
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... TRAF3 KO1 and TRAF3/p100 double KO cells from 2 experimental repeats was isolated using the RNeasy Mini Kit (Qiagen). RNA quality control was performed using a 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Genetics 2020Quote: ... in a 2 ml Eppendorf (Hamburg, Germany) tube using Stainless Steel Beads (5 mm) and the TissueLyzer (QIAGEN, Venlo, Netherlands). After an incubation time of 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... the DNA was extracted from 100 mg mouse fecal samples (9-week-old, 2 weeks after final oral gavage treatment) using QIAamp PowerFecal Pro DNA kit (Qiagen) following the manufacturer’s instruction ...
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bioRxiv - Biophysics 2022Quote: ... Purified protein was diluted in PBS buffer (pH = 7.2) and the fluorescence intensity was recorded at 60 °C in the Rotor-Gene 6600 real-time PCR cycler (Qiagen) for 18 h ...
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bioRxiv - Genomics 2020Quote: ... total RNA was extracted from cells grown in five different salinity treatments (0, 2, 12, 24, 36 parts per thousand) using the RNeasy Plant Kit (Qiagen), and 15 Illumina libraries were prepared using the Kapa mRNA HyperPrep kit and sequenced on the Illumina HiSeq2000 platform at the Beijing Genomics Institute.
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bioRxiv - Genomics 2019Quote: ... Purified products were loaded onto a 2% E-gel and gel extracted using a QiaQuick Gel Extraction kit (Qiagen 28704). The molarity of the gel-extracted PCR product was quantified using KAPA library quant (KK4824 ...
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bioRxiv - Genomics 2020Quote: The commonly up- or downregulated genes in cluster 1 and 2 compared to cluster 0 of untreated MDA-MB-231 cells were subjected to the Ingenuity Pathway Analysis (IPA) (QIAGEN) (Kramer ...
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bioRxiv - Cancer Biology 2019Quote: Mouse muscles was pulverized in liquid nitrogen and homogenized 2 × 0.5 minutes at 30 Hz using a TissueLyser II bead mill (Qiagen, USA) in ice-cold homogenization buffer ...
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bioRxiv - Genetics 2019Quote: ... purified using an E-gel electrophoresis system (Agarose 2%) followed by column purification with a MinElute PCR Purification Kit (Qiagen). In order to introduce the doped sequence in the full-length TDP-43 sequence the purified oligonucleotide was cloned into 100 ug of linearized pRS416 Gal TDP-43 by a Gibson approach (Supplementary Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... medium at 37°C and was made competent with rubidium chloride according to the method provided in the QIAexpressionist manual protocol 2 (Qiagen). When antibiotic selection was required ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from IEC isolations by adding a volume containing 2 x 106 cells in PBS to QIAshredder spin columns (QIAGEN) followed by centrifugation at 9,300 g for 1 min ...
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bioRxiv - Systems Biology 2020Quote: ... Cells in cold methanol were transferred to 2 ml polypropylene tubes and metabolite extraction was carried out with a TissueLyser II (Qiagen), using tube holders pre-cooled at -20 °C and adding the same volume of acid-washed glass beads (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from 2 mL of overnight growth in BHI broth using the DNeasy Blood and Tissue kit (Qiagen). Genome sequencing of the two isolates was performed on a Miseq platform using Illumina technology ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 to 10 g slices of liver tissue were homogenized in cell lysis solution and proteinase K (Qiagen, Germantown, USA). DNA was extracted from the liver homogenates and resuspended in buffer ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was extracted from 2 g of frozen Annona muricata seed powder with the DNEasy Mericon Food Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Alterations to the protocol included additional lysozyme treatment and bead beating with stainless steel beads for 2 min/20 Hz in a tissue lyser (Qiagen). In each sample including the blank control ...
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bioRxiv - Genomics 2020Quote: ... SARS-CoV-2 nucleic acids were isolated from 300 µl viral transport media using the QIAamp Viral RNA Mini kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Total viral RNA was extracted from patient specimens and tested for the presence of SARS-CoV-2 with an EZ1 virus extraction kit and EZ1 Advanced XL instrument with the Virus card (Qiagen), or QIASymphony DSP Virus kit and QIASymphony instrument (Qiagen).
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bioRxiv - Immunology 2021Quote: ... and distal colon (the final 3.5 cm adjacent to the rectum) and the single tumors were immediately preserved in 2 ml of RNAlater stabilization solution (Qiagen) according to manufacturer instructions and stored at −20°C ...
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bioRxiv - Microbiology 2021Quote: The cells were lysed by high speed vortexing for 2 minutes using lysing matrix E tubes (MP Biomedical) in buffer RLT (Qiagen) amended with 1% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondria were purified from approximately 2*106 cultured HeLa cells continuously expressing the mitochondrial matrix marker su9-mCherry using the Qproteome Mitochondria Isolation Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The elution supernatants were combined (a 20 μL elute sample was taken and combined with 20 μL 2 × SDS loading buffer) and RNAs were extracted using a miRNeasy kit (QIAGEN) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Nested PCR was then performed with 2 μl of cDNA in 25 μl reactions with the HotStar Taq Plus Kit (QIAGEN), using 5’ leader sequence–specific and 3’ IgG-specific primers (64) ...
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bioRxiv - Bioengineering 2021Quote: The changes in mRNA levels were compared by Relative Expression Software Tool (REST® 2009 v2.013) using 2-ΔΔCT relative quantitative analysis and also confirmed by GeneGlobe Data Analysis Center (Qiagen). Student t test has been used throughout the study ...
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bioRxiv - Bioengineering 2021Quote: ... dsDNA product was run on a 2% agarose gel and extracted with the Qiaex II Gel Extraction Kit (Qiagen, 20021). dsDNA was transcribed to RNA using 5 reactions of the Durascribe transcription kit as described above ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were harvested 2 days post-transfection and genomic DNA (gDNA) was purified with the QIAamp DNA Blood Mini Kit (Qiagen). The genomic region surrounding the pegRNA target site was then amplified by PCR with the following primers ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR fragments were incubated with DpnI for 2 h at 37°C to degrade the template plasmids before being purified using a PCR purification kit (Qiagen). The purified PCR fragments were digested overnight at 37°C using NheI and XhoI and ligated overnight at 16°C to backbone vector pYZ125 ...
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bioRxiv - Microbiology 2021Quote: The supernatant was carefully removed to a 50-mL sterile tube and mixed with 2 mL volume of 50% Ni-NTA resin (Qiagen) which was pre-equilibrated in the buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were run on a 2% agarose gel and gel bands were manually extracted and purified using QIAquick PCR & Gel Cleanup Kit (QIAGEN) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Second PCR products were pooled and gel purified from a 2% agarose gel using the MinElute Gel Extraction kit (QIAGEN). Purified product concentration was measured using a Qubit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: Tumour tissue were collected into 2 ml tubes with pre-chilled steel beads (Qiagen Stainless Steel Beads, 5 mm, 69989) and stored at −80 °C freezer ...
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bioRxiv - Microbiology 2020Quote: ... Purified extracellular WT and Δtgif2k-b tachyzoites were treated with vehicle or 5 μM sodium arsenite for 2 h at 37°C and total RNA was extracted using RNeasy (Qiagen). The RNA concentration for each sample was measured using Nanodrop One (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... the supernatant was added to 2 ml of pre-equilibrated Nickel-nitrilotriacetic acid (Ni-NTA) agarose (QIAGEN, Cat. No. 30210) 50% slurry and rotated at 4°C for 2 h ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 RNA from cell culture supernatant samples was isolated using AVL buffer and the QIAamp Viral RNA Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: For viral genome copy measurements RNA was extracted from 2 µl sucrose purified virus using the RNeasy mini kit (QIAgen). The RNA was then treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... total DNA from 2×106 of Burkitt cells was extracted by the Blood & Cell culture DNA mini kit (Qiagen #13362). For extracellular viral DNA extraction ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana seedling material was ground in a 2 ml reaction tube with the aid of a pre-cooled tissue lyser (TissuelyserII, Qiagen). RNA from ground tissue was extracted with an RNA purification Kit (Roboklon) ...
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bioRxiv - Molecular Biology 2021Quote: ... The legs and cephalothorax were separated from flash-frozen spider specimens and then homogenized with a BioMasher II (Funakoshi) and mixed with 2 mL of Buffer G2 (QIAGEN) containing 200 µg/mL RNase A ...
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bioRxiv - Synthetic Biology 2021Quote: ... ∼2.5×108 cells (0.5 ml of OD600 0.5) were mixed with 2 volumes of RNAprotect Bacteria Reagent (Qiagen, Hilden, Germany) and pelleted ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was isolated from ∼0.5 to 2×106 cells per sample using the RNeasy Plus Mini Kit (Qiagen, 74134) with RNase-free DNase treatment (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: Amplification was verified by running the PCR product on a 2% agarose gel and the PCR products pooled and cleaned up with QIAquick Nucleotide Removal Kit (Qiagen). The DNA content was quantified with Quant-iT PicoGreen dsDNA Assay Kit.
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bioRxiv - Genomics 2022Quote: ... The six samples of each technical replicate were pooled together equimolarly and the final product was purified from a 2% agarose gel with 20 ul silica beads (QIAEX II Gel Extraction Kit, Qiagen).
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bioRxiv - Microbiology 2022Quote: ... 2 throat swabs were collected with eNAT™ swabs for microbial DNA extraction using the PowerFecal DNA isolation kit (Qiagen), and in PBS for lactobacilli cultivation on MRS agar ...
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bioRxiv - Developmental Biology 2020Quote: ... run on a 2% agarose gel and a band of 300bp approximately was cut and gel purified using QIAquick Gel Extraction Kit (QIAGEN). Library concentration was determined with KAPA Library Quantification Kit for Illumina Platforms (Kapa Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates (2 × 104 cells/well) and reverse-transfected with 100 ng/well of Cignal ISRE-Luc reporter (Qiagen) using Lipofectamine 3000 ...