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Citations for Qiagen :
851 - 900 of 5692 citations for WD Repeat Containing Planar Cell Polarity Effector WDPCP Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cells were disrupted using Tissue Lyser (Qiagen) 1min 25 Hz ...
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bioRxiv - Cancer Biology 2023Quote: ... and primary cells using Rneasy Kit (Qiagen) according to the manufacturer’s instructions ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Cells were lysed in RLT buffer (Qiagen) supplemented with 1% ß-mercaptoethanol (β-ME ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected using Polyfect (QIAGEN), while U2OS cells were transfected using TransIT-LT1 Transfection Reagent (Mirus Bio) ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed with TCL buffer (Qiagen) with 1% of ß-mercaptoethanol and stored at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested in Buffer RLT (Qiagen), and gene expression was measured by quantitative PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended in Qiazol (Qiagen) and stored at − 80 °C before total RNA extraction with the miRNAeasy (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed in RLT buffer (QIAGEN) plus 1% ꞵ-mercaptoethanol (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: Cells were lysed using Buffer RLT (Qiagen) and homogenized using either syringes and needles or QIAshredder (Qiagen) ...
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bioRxiv - Biochemistry 2023Quote: Mouse EL4 genomic DNA was isolated by QIAGEN Blood & Cell Culture DNA Kit (QIAGEN, #13343). The purified genomic DNA was then fragmented to 100∼200 bp using DNase I (ZYMO RESEARCH ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected using Effectene (Qiagen #301427), incubated for 44–48 hours at 28°C ...
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bioRxiv - Microbiology 2023Quote: Infected cells were lysed in RLT (Qiagen) supplemented with 0.1% beta-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were lysed in RLT buffer (QIAGEN) supplemented with 38 mM DTT ...
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bioRxiv - Synthetic Biology 2021Quote: Total RNA was isolated from infected Hela cells (experiment) and HeLa cells co-cultured with non-engineered E.coli (control) using RNeasy Mini Kit (Qiagen) and then reverse transcribed using QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA from 3×107 sorted GFP+ cells was extracted using the Blood & Cell Culture DNA Maxi Kit (Qiagen). Amplification of sgRNA regions from the extracted genome and the original sgRNA plasmid library ...
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bioRxiv - Microbiology 2021Quote: ... 10,000 positive cells and 10,000 negative cells were sorted from three independent biological replicates directly into RLT Lysis buffer (Qiagen).
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bioRxiv - Cell Biology 2021Quote: ... cells were harvested and genomic DNA (gDNA) was isolated from surviving cells using a QIAmp DNA Mini Kit (QIAGEN) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Genomic DNA (gDNA) was extracted from each transduced cell culture using a blood & cell culture DNA mini kit (Qiagen) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA from 1-5 million total CD4+ T cells was isolated using the Gentra Puregene Cell Kit (Qiagen) or phenol-chloroform and the DNA concentration was measured by Qubit High Sensitivity Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was isolated from cells in 96-well plates using the Qiagen FastLane Cell Probe Kit (QIAGEN, 216413), according to the manufacturer’s instructions to a final volume of 40 μL per well ...
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bioRxiv - Microbiology 2019Quote: miRNA isolation from hNS1 cells and foetal neural stem cells were performed using miRNeasy mini kit (Qiagen, CA, USA) following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: Approximately 2 × 106 undifferentiated MØP cells or M-CSF differentiated MØP cells were lysed in accordance with the Qiagen RNeasy Mini kit (Qiagen). The concentration of RNA was measured using either a Nanodrop™ 2000 (Thermofisher Scientific ...
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bioRxiv - Genomics 2021Quote: Genomic DNA (gDNA) was isolated using the Blood & Cell Culture DNA Midi (5e6–3e07 cells) (Qiagen, cat. no. 13343), or Mini (<5e6 cells ...
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bioRxiv - Genetics 2021Quote: ... We extracted DNA from approximately 1-2 million cells of actively growing culture by first pelleting the cells and resuspending them in 1.0 mL Cell Lysis Solution (Qiagen). The samples were incubated with RNase A solution at 37°C for 40 min ...
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bioRxiv - Cancer Biology 2020Quote: ... SCID GFP positive FACS-sorted cells and the mT3-2D-GFP cell line using the RNeasy Micro Kit (Qiagen). RNA concentration and quality were assessed using an Agilent BioAnalyzer ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells suspended at 0.8 × 105 cells/ml were transfected with 50 nM siRNA using HiPerfect transfection reagent (Qiagen) according to the manufacturer’s protocol and analysed after 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... FACS-sorted cells (≈ 98% tdTomato+ cells) were harvested in RLT and RNA extracted with RNeasy Micro kit (Cat. # Qiagen). Around 100 000 tdTomato+ cells were sorted from 10 electroporated-brains for each sample to sequence ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were then seeded in 6-well dishes (6x105 cells/well) and reverse transfected using Polyfect (Qiagen, 301105) following the manufacturers protocol ...
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bioRxiv - Microbiology 2023Quote: ... Human DNA from whole cell extracts of A549 cells was purified using the DNeasy Blood and Tissue kit (Qiagen). RNAseA (Thermo ...
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bioRxiv - Developmental Biology 2023Quote: ... genomic DNA from sorted cell pellets was extracted using the QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (Thermo-Scientific ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions from SPL and BM were subjected to red blood cell lysis (erythrocyte lysis buffer, EL; Qiagen). Monoclonal antibodies (mAbs ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were lysed in five times the amount of red blood cell lysis solution (Qiagen, Hilden, Germany), followed by 15 min of incubation at room temperature (24°C) ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA (gDNA) was extracted from J-Lat cells 10.6 and HIV-1 negative CD4+ T cells with the QIAcube (Qiagen), using the QiaAmp DNA mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from isolated cells (microglia or non-microglia cells) with RNeasy RNA Isolation kit (Qiagen 74104) and used to generate cDNA with a High-Capacity cDNA Reverse Transcription Kit (Thermo 4368814) ...
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bioRxiv - Genetics 2023Quote: ... genomic DNA from sorted cell pellets was extracted using the QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (ThermoScientific ...
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bioRxiv - Genomics 2023Quote: Genomic DNA from each cell was amplified using the Repli-G Advanced DNA Single-Cell kit and protocol (QIAGEN) for amplifying purified genomic DNA ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 5-10×106 cells using the Gentra Puregene Cell Kit (Qiagen, cat no. 158046) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted from sorted cells (1,3×107 cells per channel) using the QIAamp DNA Mini kit (Qiagen). Gene-trap insertion site recovery and sequencing libraries were generated as previously described 60.
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from cell pellets using a genomic DNA isolation kit (Blood & Cell Culture Midi kit (Qiagen)) ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were transferred to nitrocellulose membranes before being revealed with anti-Histidine monoclonal antibodies (Qiagen). Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... Signal was detected using mouse anti-His antibody from Qiagen (Cat No./ID: 34660 1:500). Anti-mouse HRP was used for colorimetric development (Cat No./ID-A8924 1:1000) ...
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bioRxiv - Cell Biology 2023Quote: ... His-tagged DmMIC10b was detected using an anti-His antibody (Qiagen N.V., Venlo, The Netherlands, 34660).
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bioRxiv - Microbiology 2020Quote: ... and then cell lysates were collected by washing cells twice with PBS and adding 100ul of RNA lysis buffer (Qiagen) to each well.
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested by centrifugation at 4°C followed by cell lysis/RNA extraction using RNEasy plus mini kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: One colony per clone corresponding to ∼2×104 cells and 2×103 primary cells of each individual were lysed in RLT Plus (Qiagen) and stored at −80°C until processing ...
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bioRxiv - Immunology 2021Quote: ... and CD4 memory T cells were sorted on a FACSAria III cell sorter (Becton Dickinson) into QIAzol Lysis Reagent (QIAGEN). A total of 100,000 cells was sorted per sample ...
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bioRxiv - Genetics 2021Quote: ... were processed for DNA extraction using the Genomic-tip 20/G (mini prep, starting with approx. 108 cells) or 100/G (midi prep, 109 cells) kit (QIAGEN), according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2020Quote: WGA of single cells was performed by multiple displacement amplification using the REPLI-g Single Cell kit (Qiagen, Valencia, CA) as per the manufacturer’s instructions but at one quarter of the recommended volumes ...
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bioRxiv - Molecular Biology 2021Quote: ... MDA was done directly on cells (Supplementary figure 9) using the REPLI-g Single Cell Kit from Qiagen (Cat #150343) following kit-specific protocol ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from MTX-resistant HT-29 cells and control samples using the Gentra Puregene Cell kit (Qiagen) and the library for PacBio ...