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Citations for Qiagen :
851 - 886 of 886 citations for Recombinant Mouse Acy1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: PCR with forward and reverse primers (see Table 1) was performed on mouse hypothalamic cDNA (isolated with miRNeasy Mini Kit, Qiagen, Hilden, Germany). PCR products were ligated into pGEMT.easy (Promega ...
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bioRxiv - Genomics 2020Quote: ... and AH (20 weeks) old C57/Bl6/J mouse hearts (n= 4-6/group) using the RNeasy Mini Kit (Qiagen, Cat.74106) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted and purified from 20 mg (2 pellets) of stool from each mouse using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germantown, MD). The concentration of DNA in samples was determined by spectrophotometry ...
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bioRxiv - Cancer Biology 2022Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: DNA was isolated from tail biopsies using the Gentra Puregene Mouse Tail Kit according to the manufacturer’s instructions (Qiagen, Valenica, CA, USA). Genomic DNA was digested with BamHI-HF (New England Biolabs ...
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bioRxiv - Neuroscience 2019Quote: ... Total RNA and miRNA fractions were isolated from the mouse frozen tissue with the miRNeasy Micro Kit protocol (Qiagen, Toronto, ON, Canada). All RNA samples were determined to have 260/280 and 260/230 values ≥1.8 ...
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bioRxiv - Microbiology 2021Quote: ... Total genomic DNA was extracted from each ked lysate and blood (camel, mouse, and rabbit) using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Vγ4+TCRγδ+CD3+CD44+CD27− and Vγ4−TCRγδ+CD3+CD44+CD27− populations from each mouse strain were sorted using a FACSAriaIII: populations into RNA protect (QIAGEN, Hilden, Germany). Total RNA was extracted from the sorted cells according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN) ...
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bioRxiv - Microbiology 2021Quote: ... for subsequent RNA extraction and analysis using the Qiagen RT2 Profiler Mouse Innate and Adaptive Immune Response PCR Array (Qiagen, Hilden, Germany). The lungs were placed in a tissue cassette ...
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bioRxiv - Immunology 2022Quote: ... and the cDNA was loaded onto the “Mouse Toll-Like Receptor Signaling Pathway” RT2 Profiler PCR Array according to the manufacturer instructions (Qiagen, Valencia, CA). Expression of the target genes were normalized to the geometric mean of Ct values of the two housekeeping genes Gusb and Hsp90a using the ddCt method (n = 3) ...
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bioRxiv - Microbiology 2024Quote: ... Parasite genomic DNA was purified from the blood of a transfer mouse using the Qiagen QIAamp DNA Blood Kit (Qiagen, Cat# 51106), and genotyping PCR was performed to assess integration into the target locus ...
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bioRxiv - Pathology 2022Quote: ... was performed using the Qiagen Mouse Inflammatory Response and Autoimmunity (PAMM-077Z) and Mouse Extracellular Matrix and Adhesion Molecules (PAMM-013Z) RT2 Profiler PCR arrays (Qiagen, Hilden, Germany) to evaluate relative gene/mRNA expression in WT compared to mdx and treated compared to untreated muscles ...
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MK2 deficiency decreases mortality during the inflammatory phase after myocardial infarction in micebioRxiv - Physiology 2023Quote: ... First strand cDNA was synthesized from 0.5 mg of total RNA using QIAGEN RT2 First Strand Kits and transcripts for mouse cytokines and chemokines were quantified using qPCR microarrays comprising 96-well plates precoated with primers (QIAGEN PAMM-150Z). Each 96-well plate also contained primers for 5 housekeeping genes as well as positive and negative controls ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was isolated from the tail tip of a wild-type C57BL/6J mouse using the DNEasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs) ...
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bioRxiv - Immunology 2023Quote: Total cellular RNA was extracted from mouse placental tissues or HTR8 cells using a RNeasy Plus Mini Kit (Qiagen, Germantown, MD, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNAs from mouse tumors were extracted and purified using the RNeasy Mini Kit and RNase-free DNase Set (QIAGEN, Valencia, CA) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: Mouse liver RNA was extracted from 10 mg (± 2 mg) of preserved tissue using RNEasy kits and QIAshredders (QIAGEN; Germantown, MD, USA). Aliquots of RNA from each sample were assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from blood-free cranial lobes of the right mouse lung using the RNeasy Tissue Mini Kit (Qiagen, Hombrechtikon, Switzerland). Isolated RNA was reverse-transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche Diagnostics ...
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bioRxiv - Genetics 2024Quote: DNA was prepared from tail biopsies obtained at approximately 2-weeks of age (Gentra Puregene Mouse Tail Kit, Qiagen, Valencia, CA, USA). Scn1a genotype was determined as previously described (Kearney et al ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNAs and genomic DNA from lavage fluids and mouse tissue homogenate were extracted using AllPrep® DNA/RNA Micro Kit (Qiagen, #80284). The tissue homogenate was prepared by disrupting frozen tissue in pre-chilled Lysing Matrix D 2 mL tube (MP Biomedicals ...
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bioRxiv - Neuroscience 2024Quote: Vector genome copy number was quantified by qPCR analysis of total DNA extracted from mouse tissues using Qiagen DNeasy Blood and Tissue kit and Tissue Lyser II (Qiagen, Hilden, Germany). Genomic RNA was eliminated by RNase digestion (Qiagen ...
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bioRxiv - Neuroscience 2024Quote: Eye tissue was suspended in Buffer RLT/eye (1 eye/mouse; 10-18 mice/genotype) and prepared using the RNeasy kit (Qiagen, Germantown, MD). RNA quality was assessed by the 18S/28S rRNA bands in capillary electrophoresis (Bioanalyzer ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from the indicated mouse tissues and cultured cells using RNeasy® Lipid Tissue Mini Kit (Qiagen, Valencia, CA, USA). Reverse transcription reactions containing 1 μg of total RNA were performed using PrimeScript™ (Takara ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from donor cecal contents and day 0 conventionalized mouse feces using the Qiagen PowerFecal DNA Kit (Qiagen, Hilden, Germany; 12830) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: PON2 level was quantified in the mouse whole brain homogenate in 100 mg per 1 ml of QIAzol® lysis reagent (Qiagen, Hilden, Germany). mRNA 500 ng was used for cDNA synthesis in 10 μl volume according to the manufacturer’s instructions of PrimeScriptTM RT-PCR kit (Takara Bio Inc. ...
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bioRxiv - Genetics 2020Quote: ... eyes were enucleated and retinas were immediately dissected and total RNA was extracted from mouse retinal tissue using Qiagen RNeasy Mini Kit as per manufacturers protocol (Qiagen, Valencia, CA, USA) and reverse-transcribed using iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Physiology 2021Quote: RNA was extracted from mouse islets (∼300 islets per sample, pooled from ∼10 mice of the same genotype) using RNAeasy Micro Kit (Qiagen, Valencia CA, USA) as per manufactures instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: Genomic DNA was extracted and purified from mouse tails by a sodium hydroxide method or by proteinase K digestion followed by high-salt precipitation (Gentra/Qiagen, Valencia, CA, USA). In total ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from the mouse cells/tissues and human cell lines using the RNeasy® Mini Kit (Qiagen Inc., Germantown, MD). For human platelets and MEG-01 cells ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 400 ng an RNA with RNA integrity number (RIN) greater than 8 from each sample was processed with QIAseq Targeted RNA Mouse Cancer Transcriptome Panel (Qiagen, Germany, RMM-003Z). This panel consists of 416 genes selected for the association with angiogenesis ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA and RNA were extracted from male and female monkey and male mouse NAcC samples using the All Prep DNA/RNA/miRNA Universal kit (QIAGEN Sciences Inc, Germantown, MD) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8 ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA in the mouse fecal samples and GI content (200 mg) was extracted using QIAamp® PowerFecal® Pro DNA kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA from mouse forelimb buds and bamboo shark pectoral fin buds was extracted with the RNeasy Micro and Mini plus kit (QIAGEN, Cat. No. 74034 and 74134). Genomic DNA was removed with gDNA Eliminator columns included with this kit ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI ...