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Citations for Qiagen :
851 - 900 of 2279 citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Samples were then homogenized with a tissue lyser (TissueLyser II, QIAGEN) for 15 minutes at 30 Hz ...
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bioRxiv - Genetics 2022Quote: ... Approximately 200 mg of contents were homogenized using TissueLyser II (QIAGEN) in 900 uL of 50 mM sodium acetate (pH 124 = 5.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cDNA was created using the miScript II RT kit (QIAGEN). Specifically ...
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bioRxiv - Microbiology 2019Quote: ... Initial conditions were obtained with the PEGs II Suite screen (Qiagen) by vapor diffusion in sitting drops at 18°C using a Mosquito robot (TTP Labtech) ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription was performed using the miScript II RT Kit (Qiagen) and qPCR was performed using the SyBr Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Plant Biology 2021Quote: ... Frozen tissue was ground using a TissueLyser II (Qiagen Inc., USA) with 3 rounds of shaking at 30 Hz for 30 s ...
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bioRxiv - Plant Biology 2021Quote: ... Frozen tissue was ground using a TissueLyser II (Qiagen Inc., USA) with 3 rounds of shaking at 30 Hz for 30 s ...
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bioRxiv - Immunology 2021Quote: ... Tissues were thawed and homogenized using the TissueLyser II system (Qiagen). Tissue homogenates were titrated on Vero E6 cell monolayers in 24-well plates to determine viral loads ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed using the miScript II RT Kit (Qiagen, 218161). Quantitative realtime PCR was performed using StepOnePlus real-time PCR instrument ...
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bioRxiv - Physiology 2021Quote: ... and 1% β-mercaptoethanol with a TissueLyser II (Qiagen, Manchester, UK) at maximum speed for 2 minutes ...
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bioRxiv - Plant Biology 2021Quote: ... frozen in liquid nitrogen and ground using the tissuelyzer II (Qiagen). After crude protein extraction samples were centrifugated twice at 12500 rpm for 10 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The samples were homogenized (TissueLyser II bead mill, Qiagen, Hilden, Germany) using metal beads at 6 m/s for 30 seconds ...
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bioRxiv - Microbiology 2021Quote: ... Root and soil samples were pulverized using a TissueLyser II (Qiagen) with cold blocks cooled in liquid nitrogen (2 minutes grinding ...
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bioRxiv - Microbiology 2021Quote: ... lung or tracheae were homogenized using TissueLyser II (Qiagen, Hilden, Germany) for 1 min at 26 sec-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Bead-beating homogenization by TissueLyser II (Qiagen Retsch GmbH, Hannover, Germany) was performed to mechanically disrupt fecal samples before DNA extraction ...
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bioRxiv - Immunology 2022Quote: ... 2 μg/ml TPCK-trypsin) using a Tissue Lyser II (Qiagen) with the following program ...
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bioRxiv - Plant Biology 2022Quote: ... samples were shaken for 1 min in a TissueLyser II (Qiagen) and then centrifuged at 4ºC for 5 min at maximum speed in a tabletop microfuge ...
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bioRxiv - Genomics 2022Quote: ... frozen leaf tissue was grinded and homogenized using TissueLyser II (QIAGEN), then genomic DNA was isolated using the Sodium Dodecyl Sulfate (SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... or with the QIAEX II Gel Extraction Kit (Qiagen, Hilden, Germany) for Cas12a templates ...
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bioRxiv - Microbiology 2022Quote: ... Processing of tissue samples included homogenization on a TissueLyser II (Qiagen) at 50hz for 5 minutes followed by centrifugation at 10,000 rcf to pellet debris ...
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bioRxiv - Neuroscience 2023Quote: ... tissue samples were simultaneously disrupted and homogenized using TissueRuptor II (Qiagen) for 20-30 s at full speed ...
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bioRxiv - Cancer Biology 2023Quote: ... eight-ten mg of tumor were homogenized with TissueLyser II (Qiagen) in buffer RLT and transferred in a column for RNA isolation and DNA digestion ...
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bioRxiv - Microbiology 2023Quote: Frozen samples were homogenised with a bead mill (TissueLyser II, Qiagen) for 2 min (frequency 30−1) ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted using TissueLyser II (Qiagen, Valencia, CA, USA) and NucleoSpin RNA (Macherey-Nagel ...
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bioRxiv - Immunology 2023Quote: ... miScript primers and miScript II RT Kit were purchased from Qiagen. 100 ng total RNA was reverse transcribed according to manufacturer’s instruction ...
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bioRxiv - Zoology 2024Quote: ... Sample homogenization was achieved using a TissueLyser II (Qiagen, Hilden, Germany) with 5mm stainless steel beads (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... with U6 primers included in the miScript II RT kit (QIAGEN) and Gapdh primers provided by Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was harvested from mouse ear skin using TissueLyser II (Qiagen) and TRIzol (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The mouse aorta was homogenized by TissueLyser II (Qiagen, Venlo, Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... the tissues were homogenized in RIPA lysis with TissueLyser II (Qiagen), sonicated with Bioruptor sonication device (Diagenode ...
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bioRxiv - Immunology 2023Quote: ... and MN/MCA1 tumor tissues were disrupted by TissueLyser II (QIAGEN) with stainless steel beads following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... frozen with liquid nitrogen and ground in a TissueLyser II (Qiagen). Genomic DNA was extracted using a 96-well plate column-based kit ...
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bioRxiv - Developmental Biology 2023Quote: MiRNAs were reverse-transcribed into cDNA using miScript II (Qiagen, 218160) and RT-qPCR was performed using the miScript SYBR Green PCR Kit (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA from the control human TSCs as well as GATA2-KD and GATA3-KD human TSCs were isolated using RNeasy Mini Kit (Qiagen, Germantown, MD) per the manufacturer’s protocol with on-column deoxyribonuclease digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Targeted knockdown was conducted with 100nM ON-TARGETplus smartpool human c-MYC (mixture of 4 individual siRNA) and/or human KLF6-SV1 siRNA (Dharmacon, Lafayette, CO) with HiPerfect (Qiagen, Valencia, CA).
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bioRxiv - Cell Biology 2020Quote: RNA FISH was performed using a FITC-labeled DNA oligo probe complementary to alpha satellite (Integrated DNA Technologies, Newark, NJ) or a biotinylated locked nucleic acid (LNA) oligo probe complementary to HSATII (QIAGEN, Hilden, Germany) (see Key Resources Table) ...
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bioRxiv - Cell Biology 2021Quote: Oligonucleotides for siRNA included: human βPix (synthesized by Qiagen), human myosin-18A (synthesized by Qiagen)
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bioRxiv - Microbiology 2019Quote: ... Short CAP was expressed and His tag purified using Ni-NTA affinity purification under native conditions using the standard manufacturer’s protocol (Qiagen, Hilden, Germany). The shortCAP recombinant protein was used to immunize female New Zealand white rabbits (Covalab ...
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bioRxiv - Cell Biology 2019Quote: ... Input (3% of total binding reaction) and bound samples (50% of binding reaction) were subjected to SDS-PAGE followed by immunoblot analysis with His (Qiagen 34660) and GST (BioLegend MMS-112P ...
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bioRxiv - Synthetic Biology 2021Quote: ... Clarified supernatants containing TtgR and SmtB were purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The eluted fractions from the FPLC (for TetR ...
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bioRxiv - Synthetic Biology 2020Quote: ... For SARS-CoV-2 antigen detection the blots were incubated with 1:2000 Anti-His mouse monoclonal primary antibody (Qiagen, #34660) and then incubated with 1:10000 peroxidase labelled anti-mouse IgG secondary antibody (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Clarified supernatant containing mRFP1 was then purified using His-tag affinity chromatography with a gravity column charged with Ni-NTA Agarose (Qiagen #30210). The elution from the gravity column was concentrated and buffer exchanged (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: Nunc MaxiSorp™ 96-well plates were coated with 75 ng/well of penta-His antibody in PBS solution (Qiagen, 34660) and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: Total RNA from 1,000 H3K27me3 HI/LO β-cells or from 50,000 sorted βHI and βLO cells was extracted using the miRNeasy FFPE Kit (QIAGEN, 217504), followed by the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (E6420L) ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated for 1 hour with monoclonal α-His antibodies conjugated to the horseradish peroxidase (1:5000 dilution; Qiagen). Blots were visualized with the addition of Lumina Forte Western HRP Substrate (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were clarified by additional centrifugation at 14,000 xg for 30 minutes and supernatants were incubated 1 hour at 4 °C with 5 mL of His-Pur™ Ni-NTA resin (Qiagen) previously washed 3 times with 25 mL of Lysis Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-fused p300 was expressed from pFastBac1 vector in High-Five cells and purified on Ni-NTA agarose beads (Qiagen, 30210) in BC buffer (20 mM HEPES pH7.9 ...
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bioRxiv - Cancer Biology 2022Quote: Whole tissue protein was extracted by Qproteome Mammalian Protein Prep Kit (Qiagen, 37901) according to the manufacture’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein were extracted using the AllPrep DNA/RNA/Protein kit (Qiagen, #47054). Sample concentrations were measured with Qubit high sensitivity dsDNA and RNA platform ...