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Citations for Qiagen :
851 - 900 of 1196 citations for Mouse Anti Hantavirus Nucleocapsid Protein Antibody 4957 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Viral RNA was isolated from 30 µl of mouse sera using the QIAmp Viral RNA Mini Kit (Qiagen) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was extracted from HKC-8 or mouse kidneys using the miRNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Xenograft and HCC mouse samples were homogenized in RIPA buffer using metal beads and the LT tissuelyzer (Qiagen). All samples were sonicated (ultrasound probe ...
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bioRxiv - Microbiology 2021Quote: Mouse lung tissues were homogenized in 1 ml of MEM-free media using TissueRuptor (Qiagen, Chadstone, Victoria, Australia). The homogenate was obtained by centrifugation at 6,000 × g for 5 min at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: Microdissected mouse and human brain tissue was flash frozen and homogenized in Trizol using a TissueLyser II (Qiagen). Phenol chloroform extraction was then used together with the RNeasy mini kit (QIAGEN ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from the podocytes or mouse glomeruli using the RNeasy Plus Mini Kit (Qiagen, #74134). 1 μg of RNA was reverse transcribed using Oligo dT primers and a high-capacity cDNA Synthesis Kit according to the manufacturer’s instructions (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... mouse and macaque retina or RPE/choroid tissue were isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). Briefly ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted from mouse cecal contents or feces using the QIAmp PowerFecal DNA kit (QIAGEN, CA, USA). A multiplexed amplicon library converting the 16S rDNA V4 region was generated from the DNA samples ...
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bioRxiv - Systems Biology 2023Quote: ... RNAs were extracted from isolated mouse LV or SAN with RNeasy Mini Kit (Qiagen, Valencia, CA, United States) and DNAse ...
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bioRxiv - Molecular Biology 2023Quote: We also used the RT2 Profiler PCR array mouse fibrosis (cat. #PAMM 120-ZE-4) kit from Qiagen to detect the expression of 84 fibrosis-related genes ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from a KI mouse brain hemisphere using the RNeasy Plus Universal Mini Kit (Qiagen, 73404), 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche ...
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bioRxiv - Cancer Biology 2023Quote: Approximately 1 cm of the mouse distal ileum was cut into small pieces and stored in RNAlater (Qiagen). RNA was prepared using a combination of Trizol (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... The cDNA was analyzed with the Qiagen RT2 Mouse Cytokines and Chemokines array (PAMM-150Z, Qiagen, Hilden, Germany). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133 ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was conducted with the Mouse Inflammasomes (96-well format) RT2 Profiler PCR Array (Cat. # PAMM-097Z, Qiagen) according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: The RNA was isolated from cultured cell lines or mouse tissues using the RNeasy Mini Kit from QIAGEN. This kit allows for efficient isolation of high-quality RNA from various sources.
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from mouse LV using the Qiagen RNeasy Mini Kit for mRNA analyses (Qiagen, 74106). List of oligos is accessible and details can be found in the Supplemental Material.
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from PGK12.1 mouse embryonic stem cells using the DNeasy blood and tissue kit (Qiagen), following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: Bulk RNA was isolated from mouse hearts (normal, ischemic, or non-ischemic) using the miRNeasy Mini Kit (Qiagen). Total RNA samples (100-500 ng ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from primary mouse endothelial cells using a commercially available kit (Qiagen, Valencia, CA, USA) followed by DNase I treatment ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant both N-and C-terminally His-tagged proteins were purified using a Ni-NTA Superflow cartridge (1ml, Qiagen) and the Fast Protein Liquid Chromatography (FPLC ...
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bioRxiv - Cell Biology 2019Quote: ... The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose, Qiagen) following the purification hybrid method from the ProBond purification system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA expression levels were normalized to the housekeeping human TATA-binding protein (TBP) mRNA (RT2 qPCR Primer Assays, Qiagen) in the GAL4 assay and to the human housekeeping hypoxanthine guanine phosphoribosyltransferase (HPRT ...
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bioRxiv - Microbiology 2021Quote: ... Cln1-His6 and its associated proteins were co-purified following the Ni-NTA Spin Kit procedure (Qiagen, Valencia, CA). Cell lysate from the CLN1-His6 strain was used as a negative control ...
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bioRxiv - Biophysics 2021Quote: ... The target protein was eluted by 0.2 mg/ml FLAG peptide and load to Ni-NTA affinity gel (Qiagen). After washed by Lysis Buffer supplied with 0.02% GDN and 0.004% CHS ...
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bioRxiv - Biochemistry 2020Quote: ... and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen) equilibrated with 10 mM imidazole in the lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: The genes encoding protein groups significantly altered in epilepsy and control were analyzed using Ingenuity Pathway Analysis (IPA, Qiagen). We analyzed all differentially expressed proteins in the hippocampus (n=777) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed and recombinant proteins were purified under native conditions using the Ni-NTA Fast Start Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The pulldown protocol was based on the principle of immobilization of recombinant protein using Ni-NTA agarose resin (Qiagen). The experiments were performed in biological triplicates and designed to contain 4 control samples (resin Ni-NTA with i ...
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bioRxiv - Microbiology 2021Quote: ... and H2A.Z (fused with a maltose-binding protein -MBP) purification were performed by affinity chromatography using Ni-NTA agarose (Qiagen) and amylose resin (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was clarified by centrifugation at 13,000 rpm and protein was purified using Ni- NTA superflow resin (Qiagen), according to the manufacturer’s recommendations for a centrifugation-based protocol ...
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bioRxiv - Microbiology 2022Quote: ... Headless HA stalk proteins were expressed in 293F cells and purified using nickel-nitrilotriacetic acid agarose (no. 1018244, Qiagen) in 5-ml polypropylene columns (no ...
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bioRxiv - Biochemistry 2021Quote: ... was transformed with an N-terminally His-tagged fusion protein of cytHPPK/DHPS (At1g69190) in the vector pQE30 (Qiagen). Overnight cultures grown at 30°C in LB broth supplemented with 35 µg/mL kanamycin and 100 µg/mL ampicillin were sub-cultured to an OD600 of 0.6 - 0.7 and induced overnight at 16°C with 100 mM isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Biochemistry 2021Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Microbiology 2022Quote: ... Tissue lysate was further used for DNA extraction with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). Total DNA extraction of seawater filters was done using the entire filter ...
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bioRxiv - Microbiology 2022Quote: ... N-terminal His-tagged SlPR1 and SlChi3 proteins were purified using Ni-nitrilotriacetic acid (Ni-NTA) affinity resin (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The solubilized protein was incubated for 1 hour with washed and pre-equilibrated Strep-Tactin Superflow plus resin (Qiagen). The resin was then loaded into an Econo-Column Chromatography Column (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... and 8 M urea [pH 8.0]) and His-tagged Tax proteins were precipitated with Ni-nitrilotriacetic acid (NTA) agarose (Qiagen). After washing in buffer C (100 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2020Quote: ... proteins with log fold change >1 and Z-score > 2 were further analyzed in Ingenuity Pathway Analysis (IPA, Qiagen) to determine pathways enriched by the bait proteins ...
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bioRxiv - Synthetic Biology 2021Quote: ... After spinning down the insolubilized protein fraction the supernatant was loaded onto an equilibrated mini NiNTA spin column (Qiagen) and the flow-through was reloaded twice to maximize protein binding ...
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bioRxiv - Genomics 2021Quote: ... DNA gets separated from proteins and cellular debris using proteinase K (20mg mL-1; Qiagen, Inc., Valencia, CA, USA), precipitated with ethanol and cleaned up ...
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bioRxiv - Genomics 2022Quote: ... DNA and RNA was extracted from fresh-frozen tissue samples using the AllPrep DNA/RNA/Protein minikit (Qiagen, USA) as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant SlERF.D7-6XHIS protein was purified by Ni2+gravity flow chromatography according to the manufactureer’s protocol (Ni-NTA Agarose, Qiagen) as described ...
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bioRxiv - Plant Biology 2022Quote: ... Recombinant RIN-6XHIS protein was purified by Ni2+ gravity flow chromatography according to the manufacturer’s protocol (Ni-NTA Agarose, Qiagen). For the EMSA assay ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from FACS-purified astrocytes from a single mouse brain using the QIAshredder and the All-prep DNA/RNA/protein Mini Kit according to the manufacturer’s protocol (Qiagen). Total RNA concentration was measured using a NanoVue PlusTM spectrophotometer (Biochrom) ...
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bioRxiv - Biochemistry 2022Quote: ... Culture medium was harvested 72 h post-transfection and secreted His-tagged proteins purified using Ni-NTA agarose (Qiagen) in batch mode ...
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bioRxiv - Genetics 2023Quote: ... Precipitated proteins were resuspended in 6M guanidine hydrochloride and incubated with 25 µL of Ni-NTA agarose beads (Qiagen) overnight ...
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bioRxiv - Immunology 2023Quote: ... The supernatant containing target proteins was passed through a polypropylene column containing 2 ml Ni-NTA resin from Qiagen. After the target protein bound to the Ni-NTA resin ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were analyzed by NTA and then processed with AllPrep DNA/RNA/Protein Mini Kit (cat. no. 80004, Qiagen).
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bioRxiv - Microbiology 2023Quote: ... Total RNA was isolated from these samples according to the manufacturer’s instruction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen). RNA was quantified using a Nanodrop-1000 spectrophotometer (Thermo-Scientific ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from hippocampus and pre-frontal cortex was purified with AllPrep DNA/RNA/Protein Mini Kit (QIAGEN, 80004) and DNAseI digested (QIAGEN ...